##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574527 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03194412022092 33.0 31.0 34.0 30.0 34.0 2 32.14074701877277 34.0 31.0 34.0 30.0 34.0 3 32.19991633414604 34.0 31.0 34.0 30.0 34.0 4 35.63560102496498 37.0 35.0 37.0 35.0 37.0 5 35.67811330003531 37.0 35.0 37.0 35.0 37.0 6 35.75456345961802 37.0 36.0 37.0 35.0 37.0 7 35.672925939405566 37.0 36.0 37.0 33.0 37.0 8 35.66352265872527 37.0 36.0 37.0 33.0 37.0 9 37.34241901522105 39.0 38.0 39.0 34.0 39.0 10-11 37.25269826263232 39.0 37.0 39.0 34.0 39.0 12-13 37.155406604786045 39.0 37.0 39.0 34.0 39.0 14-15 38.473578253403446 40.0 38.0 41.0 34.0 41.0 16-17 38.400674958934204 40.0 38.0 41.0 34.0 41.0 18-19 38.31059045020697 40.0 38.0 41.0 33.5 41.0 20-21 38.20202099259398 40.0 38.0 41.0 33.5 41.0 22-23 38.12001311308835 40.0 38.0 41.0 33.0 41.0 24-25 38.168639909389185 40.0 38.0 41.0 33.0 41.0 26-27 38.08965841104016 40.0 38.0 41.0 33.0 41.0 28-29 37.96447211468359 40.0 38.0 41.0 33.0 41.0 30-31 37.803367764253395 40.0 38.0 41.0 33.0 41.0 32-33 37.604089994006664 40.0 37.5 41.0 32.5 41.0 34-35 37.29779450749594 40.0 37.0 41.0 31.5 41.0 36-37 37.04652505100937 40.0 37.0 41.0 31.0 41.0 38-39 36.81262985394987 40.0 36.5 41.0 30.0 41.0 40-41 36.66032459554707 40.0 36.0 41.0 30.0 41.0 42-43 36.47209856412459 40.0 36.0 41.0 30.0 41.0 44-45 36.235629107793386 39.5 36.0 41.0 29.5 41.0 46-47 35.91150083102643 39.0 35.0 41.0 28.0 41.0 48-49 35.91320580440601 39.0 35.5 41.0 28.0 41.0 50-51 34.934857742800915 38.0 34.0 40.0 26.0 40.5 52-53 35.094376559267005 38.5 35.0 40.0 26.5 40.5 54-55 35.598116081128694 39.0 35.0 41.0 27.0 41.0 56-57 35.477934976016954 39.0 35.0 41.0 27.0 41.0 58-59 35.28573442811049 39.0 35.0 41.0 26.5 41.0 60-61 34.99685689837395 38.5 35.0 41.0 26.0 41.0 62-63 34.63301511306737 38.0 34.5 40.0 26.0 41.0 64-65 34.26471192572461 37.5 34.0 40.0 25.0 41.0 66-67 33.87782920901587 37.0 34.0 40.0 23.5 41.0 68-69 33.47266060911383 36.0 33.5 39.0 22.5 41.0 70-71 33.06879321910394 36.0 33.0 39.0 22.0 41.0 72-73 32.62132869455243 35.0 33.0 38.5 20.5 40.0 74-75 32.167135555385514 35.0 32.0 37.5 19.5 39.5 76-77 31.73005235524817 35.0 32.0 37.0 18.0 39.0 78-79 31.30545397271033 35.0 31.5 36.5 16.0 39.0 80-81 30.884922162401097 35.0 31.0 36.0 11.5 38.0 82-83 30.464993181271744 35.0 31.0 35.5 7.0 37.0 84-85 30.09213342878129 34.0 31.0 35.0 4.5 37.0 86-87 29.70830234058528 34.0 30.0 35.0 2.0 36.0 88-89 29.311031890518144 34.0 30.0 35.0 2.0 36.0 90-91 28.941157734993652 34.0 29.5 35.0 2.0 36.0 92-93 28.5373402570647 34.0 29.0 35.0 2.0 35.0 94-95 28.089446139038355 34.0 29.0 35.0 2.0 35.0 96-97 27.531034244348575 34.0 27.5 35.0 2.0 35.0 98-99 26.837980724226234 34.0 26.0 35.0 2.0 35.0 100-101 24.259852003882656 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23469797283624416 1101 2 0.2286456592784063 1101 3 0.21080001008603944 1101 4 0.09647662939286761 1101 5 0.21268543544027807 1101 6 0.20259125279115864 1101 7 -0.006683512705734529 1101 8 0.20009083261385996 1101 9 0.28718637916428946 1101 10-11 0.3265858711780609 1101 12-13 0.21827120378036824 1101 14-15 0.2553546115664318 1101 16-17 0.35932679625581443 1101 18-19 0.40036837040440787 1101 20-21 0.337849110093174 1101 22-23 0.3278917684048537 1101 24-25 0.35408123822833915 1101 26-27 0.24211769611960676 1101 28-29 0.198946349799364 1101 30-31 0.178095173522955 1101 32-33 0.23662678101275247 1101 34-35 0.25568186489874734 1101 36-37 0.08290082207565774 1101 38-39 0.1750496556830612 1101 40-41 0.10089378624564205 1101 42-43 -0.028549249532943577 1101 44-45 0.05482341098019816 1101 46-47 0.0027746186210890755 1101 48-49 0.21520511565625355 1101 50-51 -0.022976769376732875 1101 52-53 0.24932035215346815 1101 54-55 0.18816782976556823 1101 56-57 0.1692757849821973 1101 58-59 0.08492338749118034 1101 60-61 0.01119975432503395 1101 62-63 0.1147038054511782 1101 64-65 0.08882087987969811 1101 66-67 0.036984090632905975 1101 68-69 0.1151314174737692 1101 70-71 0.1117835829923024 1101 72-73 -0.09991090190590768 1101 74-75 -0.07614356992272775 1101 76-77 0.007187082881802809 1101 78-79 0.0722292923810599 1101 80-81 -0.14712056117323158 1101 82-83 -0.028685628349862213 1101 84-85 -0.1419386783129859 1101 86-87 -0.11424971097039816 1101 88-89 -0.09105146720063928 1101 90-91 -0.3072693388913237 1101 92-93 -0.4234798404474276 1101 94-95 -0.4711889513577958 1101 96-97 -0.6070755968397599 1101 98-99 -0.7581870732734046 1101 100-101 -0.9376421057166375 1102 1 0.09246145310040532 1102 2 0.12263761991117406 1102 3 0.2877366152687557 1102 4 0.25279964842295044 1102 5 0.18439645191988774 1102 6 0.2730285239422443 1102 7 0.35901956387913714 1102 8 0.3431700833472249 1102 9 0.3578755146786534 1102 10-11 0.26328833799652074 1102 12-13 0.35013831250281413 1102 14-15 0.45258748563524165 1102 16-17 0.5218436692781836 1102 18-19 0.46273593520825074 1102 20-21 0.43062979412574975 1102 22-23 0.5112068485349184 1102 24-25 0.5869269573796743 1102 26-27 0.49353274213061127 1102 28-29 0.4309481442832208 1102 30-31 0.4790134041459666 1102 32-33 0.5001664302528255 1102 34-35 0.5012536974706165 1102 36-37 0.4709780485436923 1102 38-39 0.43549571025566536 1102 40-41 0.3994460635192567 1102 42-43 0.4209523435191045 1102 44-45 0.30779798680460146 1102 46-47 0.28638761888070974 1102 48-49 0.27314396593323664 1102 50-51 0.31884117009027335 1102 52-53 0.3439071583203557 1102 54-55 0.3738682603820038 1102 56-57 0.36983611355974944 1102 58-59 0.411513082339404 1102 60-61 0.36268741525910286 1102 62-63 0.300841019803471 1102 64-65 0.4335188465623716 1102 66-67 0.4131104923247335 1102 68-69 0.30723479327191683 1102 70-71 0.14541652748764022 1102 72-73 0.19059255824490862 1102 74-75 0.2660125760352585 1102 76-77 0.15874526981648884 1102 78-79 0.016458843073735352 1102 80-81 -0.08193663155201492 1102 82-83 -0.12138414809962939 1102 84-85 -0.04757936574752364 1102 86-87 -0.03449315996040525 1102 88-89 0.053102882284040476 1102 90-91 -0.11394762394568048 1102 92-93 -0.20185093851437585 1102 94-95 -0.2055641596283344 1102 96-97 -0.41042583055039117 1102 98-99 -0.50580077779831 1102 100-101 -0.597689872409866 1103 1 0.22747985494634548 1103 2 0.21391904279667528 1103 3 0.36346348528608985 1103 4 0.2198855122434935 1103 5 0.2907024112438279 1103 6 0.305970281240306 1103 7 0.29017749911746193 1103 8 0.2220472288610722 1103 9 0.31969385734200273 1103 10-11 0.3782843261739117 1103 12-13 0.319491619059761 1103 14-15 0.42330301554157757 1103 16-17 0.525859934447098 1103 18-19 0.5958577512948722 1103 20-21 0.5526614413604136 1103 22-23 0.5193856935394976 1103 24-25 0.5028770523924564 1103 26-27 0.39274675712482576 1103 28-29 0.4556556211848175 1103 30-31 0.4178327230130421 1103 32-33 0.44020581062807906 1103 34-35 0.27923282826685636 1103 36-37 0.1662643791031968 1103 38-39 0.23250878566827282 1103 40-41 0.2479787060799694 1103 42-43 0.127425323691007 1103 44-45 0.188262284287525 1103 46-47 0.18828590440118376 1103 48-49 0.1257146035638712 1103 50-51 0.10303640465538422 1103 52-53 0.011813330732543648 1103 54-55 0.12719283865774145 1103 56-57 0.09013751984168294 1103 58-59 0.18991019985354995 1103 60-61 0.27057716566076806 1103 62-63 0.15942311382421082 1103 64-65 0.10978475333279647 1103 66-67 0.04473680833582705 1103 68-69 -0.023437126171764078 1103 70-71 0.10344961797403585 1103 72-73 -0.02808103700873943 1103 74-75 -0.12311779912133858 1103 76-77 -0.11789848613026166 1103 78-79 -0.019202338901532556 1103 80-81 0.013054133722945949 1103 82-83 -0.03416505777316203 1103 84-85 -0.012739091143384229 1103 86-87 -0.10138957767516033 1103 88-89 -0.04560620707278673 1103 90-91 -0.07365893883068253 1103 92-93 -0.2701129597853438 1103 94-95 -0.41936629738702536 1103 96-97 -0.4445840759951274 1103 98-99 -0.5204489523514155 1103 100-101 -0.5635257357475254 1104 1 0.1644889359448669 1104 2 0.18898992180708518 1104 3 0.20181156624956742 1104 4 0.15703035629122297 1104 5 0.21260563948992228 1104 6 0.24910659213768582 1104 7 0.16456144661258065 1104 8 0.16497491474669346 1104 9 0.233549251248526 1104 10-11 0.2517985416350328 1104 12-13 0.21506511648930626 1104 14-15 0.376228377222823 1104 16-17 0.31929202802029977 1104 18-19 0.37564160028799165 1104 20-21 0.42694983823121646 1104 22-23 0.3847335586839975 1104 24-25 0.43216442550657774 1104 26-27 0.46273924957576895 1104 28-29 0.4325418698267285 1104 30-31 0.4893421277400236 1104 32-33 0.3415427815257246 1104 34-35 0.31943455444974944 1104 36-37 0.3421450404315749 1104 38-39 0.3853675570495696 1104 40-41 0.39611030400737235 1104 42-43 0.35050716335410215 1104 44-45 0.2668882736884157 1104 46-47 0.41519428832285143 1104 48-49 0.49489989614614416 1104 50-51 0.4769876074311412 1104 52-53 0.4863869960212881 1104 54-55 0.44767138496013814 1104 56-57 0.38608565688119967 1104 58-59 0.46108434732246906 1104 60-61 0.5618060610549662 1104 62-63 0.5589777335493338 1104 64-65 0.5260998379725734 1104 66-67 0.41311370205512077 1104 68-69 0.33550930829581915 1104 70-71 0.37950754600117875 1104 72-73 0.2809223958042324 1104 74-75 0.2001712820985304 1104 76-77 0.1909457571290858 1104 78-79 0.18028725843927873 1104 80-81 0.08699648238680524 1104 82-83 0.07396901192714367 1104 84-85 0.10100009254438902 1104 86-87 0.022410828507222647 1104 88-89 0.040594751753747715 1104 90-91 0.15101590313264524 1104 92-93 0.05004785302212866 1104 94-95 -0.1220588697094378 1104 96-97 -0.13750207769585643 1104 98-99 -0.05566922605414959 1104 100-101 -0.07527302094769439 1105 1 0.12078005007246873 1105 2 0.08292598122293526 1105 3 0.18162752488979095 1105 4 0.11107302796990126 1105 5 0.1391984647148874 1105 6 0.18672422840834457 1105 7 0.23540907032644043 1105 8 0.20066351698014273 1105 9 0.23053294348125775 1105 10-11 0.2112226961933672 1105 12-13 0.22988936154222017 1105 14-15 0.23067586492707193 1105 16-17 0.2714166428159004 1105 18-19 0.23694766744139883 1105 20-21 0.2543680918998703 1105 22-23 0.22672947304076985 1105 24-25 0.1848769873869358 1105 26-27 0.23513010051940597 1105 28-29 0.2993839032240473 1105 30-31 0.1735066458295762 1105 32-33 0.13616129864606563 1105 34-35 0.037340166988343526 1105 36-37 -0.026504742836564787 1105 38-39 0.11446049606210806 1105 40-41 0.08524934706726128 1105 42-43 0.05127984791085538 1105 44-45 -0.08973510015329822 1105 46-47 -0.06334644628972796 1105 48-49 -0.06330793618719355 1105 50-51 -0.023523499574693574 1105 52-53 0.08317925739536491 1105 54-55 0.10059197729808744 1105 56-57 0.050488352441881545 1105 58-59 0.04201917247782916 1105 60-61 -0.01557055974861754 1105 62-63 0.08028519292716396 1105 64-65 0.07316318150662227 1105 66-67 -0.08435164628210146 1105 68-69 -0.025268784517358256 1105 70-71 -0.11222317637588475 1105 72-73 -0.1847217891153008 1105 74-75 -0.1334509378033104 1105 76-77 -0.02118533686480717 1105 78-79 -0.11117894129673189 1105 80-81 -0.039295943644575004 1105 82-83 -0.2157381657929811 1105 84-85 -0.16070918422179403 1105 86-87 -0.05943563591207379 1105 88-89 -0.12647298264196039 1105 90-91 -0.2140309639607949 1105 92-93 -0.3009538792263413 1105 94-95 -0.43779609270363196 1105 96-97 -0.37440860856709435 1105 98-99 -0.44926927745935785 1105 100-101 -0.26056979232570043 1106 1 0.2194883644628618 1106 2 0.3220847912840554 1106 3 0.2860331211655236 1106 4 0.29563742950673344 1106 5 0.26870508428648776 1106 6 0.3034917640845052 1106 7 0.25442023860351526 1106 8 0.32149015599868846 1106 9 0.42878014729767955 1106 10-11 0.31434265831433805 1106 12-13 0.35420981573390264 1106 14-15 0.39643918481349516 1106 16-17 0.494117552831284 1106 18-19 0.4209034949067103 1106 20-21 0.5154748258491111 1106 22-23 0.5070402332475012 1106 24-25 0.45670576790050177 1106 26-27 0.4763969768784122 1106 28-29 0.43129404751953615 1106 30-31 0.5202073515012131 1106 32-33 0.35626805453826904 1106 34-35 0.3249156730305387 1106 36-37 0.3072801353149046 1106 38-39 0.45625652329056976 1106 40-41 0.37990402671044166 1106 42-43 0.30001958529485506 1106 44-45 0.4348467494593038 1106 46-47 0.5150533227606999 1106 48-49 0.47314530783832964 1106 50-51 0.3891775784414193 1106 52-53 0.34770892049561297 1106 54-55 0.3037568024710353 1106 56-57 0.3100517939745373 1106 58-59 0.3851898913906595 1106 60-61 0.43929278708648667 1106 62-63 0.44908399456767967 1106 64-65 0.14642641396408607 1106 66-67 0.24500949837200636 1106 68-69 0.26557090712929465 1106 70-71 0.36674998945043313 1106 72-73 0.24952032530759283 1106 74-75 0.17523985993958036 1106 76-77 0.16440266911739343 1106 78-79 0.21022685452272327 1106 80-81 0.26571760955713586 1106 82-83 0.43249950655348 1106 84-85 0.5153078373378435 1106 86-87 0.44367177758298126 1106 88-89 0.4995474503610602 1106 90-91 0.5344306293581269 1106 92-93 0.5701468752683638 1106 94-95 0.4892708477013663 1106 96-97 0.4056130360983339 1106 98-99 0.35755928362467415 1106 100-101 0.2834283987521573 1107 1 0.12038770601288462 1107 2 0.1432252391393618 1107 3 0.20841456405528191 1107 4 0.21828322194044603 1107 5 0.14518405898116526 1107 6 0.20220521959016224 1107 7 0.24890973001777184 1107 8 0.2478309856886085 1107 9 0.3130147309203082 1107 10-11 0.340407930039639 1107 12-13 0.3271606756751808 1107 14-15 0.37840639766156414 1107 16-17 0.321151450088621 1107 18-19 0.23748118829507803 1107 20-21 0.16284034269558134 1107 22-23 0.26441143726939487 1107 24-25 0.3081597066599002 1107 26-27 0.2560963634573241 1107 28-29 0.24082889968875776 1107 30-31 0.336426342893013 1107 32-33 0.31322893926495254 1107 34-35 0.38891355888489443 1107 36-37 0.4201481180139126 1107 38-39 0.5338491334763447 1107 40-41 0.4813082677555798 1107 42-43 0.4568262850998437 1107 44-45 0.45107480194009497 1107 46-47 0.42815536461284864 1107 48-49 0.40860123515700053 1107 50-51 0.3284564881955774 1107 52-53 0.44817492400552084 1107 54-55 0.49781946291087564 1107 56-57 0.3916921243773075 1107 58-59 0.44078585401667425 1107 60-61 0.36110128284160226 1107 62-63 0.4241045080922845 1107 64-65 0.3285609722742322 1107 66-67 0.20098350524737185 1107 68-69 0.40757012823353733 1107 70-71 0.3696391266200294 1107 72-73 0.39160145410129843 1107 74-75 0.4825063490067336 1107 76-77 0.43944494877676377 1107 78-79 0.5078283175713771 1107 80-81 0.5291525854256847 1107 82-83 0.43574880980942154 1107 84-85 0.4084697084055442 1107 86-87 0.3827961445268251 1107 88-89 0.37391097799754647 1107 90-91 0.38822060470285535 1107 92-93 0.34985781188889575 1107 94-95 0.2442969052459496 1107 96-97 0.3217570498256386 1107 98-99 0.30995822726762867 1107 100-101 0.3597622984171096 1108 1 0.03007491555964492 1108 2 0.08856707751012749 1108 3 0.13014009511334024 1108 4 0.05699237931699486 1108 5 0.09863444591695014 1108 6 0.09766767262083675 1108 7 0.24870669706945137 1108 8 0.2603807203810504 1108 9 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2316 98-99 -1.2063370702946656 2316 100-101 -0.8143910883228536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9555.0 3 2619.0 4 2574.0 5 2843.0 6 3288.0 7 3762.0 8 4674.0 9 5661.0 10 6600.0 11 8071.0 12 10112.0 13 14893.0 14 13335.0 15 12739.0 16 15063.0 17 18475.0 18 18088.0 19 13597.0 20 13784.0 21 14709.0 22 15509.0 23 16954.0 24 19280.0 25 21848.0 26 24980.0 27 29360.0 28 34127.0 29 40844.0 30 48881.0 31 60111.0 32 74926.0 33 99129.0 34 137609.0 35 204271.0 36 335771.0 37 548956.0 38 559428.0 39 108051.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17712725906549 18.52484367960516 14.49940839102625 23.7986206703031 2 15.355463838786237 19.574806830250562 40.81385432226186 24.25587500870134 3 19.494356855766956 23.338787750581737 29.4078509388649 27.759004454786407 4 13.141883811957136 16.27029265760169 36.6496528203124 33.93817071012878 5 14.418925946396504 36.43146664571494 34.196373457840544 14.953233950048016 6 32.874939984332755 35.29321662594349 17.351418702339785 14.480424687383978 7 29.11623172707455 31.926254732133174 20.556771407009915 18.400742133782366 8 26.003874199711806 35.499007343249275 19.608334117453104 18.88878433958581 9 25.960022626501388 17.40382279017419 20.370032715121848 36.266121868202575 10-11 25.255050469708067 26.24135188217566 27.098291136583114 21.405306511533155 12-13 25.912811020563836 23.387556728121403 27.670454567544418 23.029177683770342 14-15 22.763869198378675 25.09965131516409 25.785279578677834 26.351199907779403 16-17 22.668812539360434 28.170973961856333 26.463955558665514 22.69625794011771 18-19 22.487316759567907 26.992727245157607 28.355987265145966 22.163968730128524 20-21 23.08500054729685 26.17768463686222 28.561526956265443 22.17578785957549 22-23 22.728958144850708 26.048255051917668 28.46680187834177 22.755984924889855 24-25 22.65008349342641 26.271561032459857 28.543433986326406 22.534921487787322 26-27 22.538675716702052 26.485743744037286 28.458626307615898 22.51695423164476 28-29 22.36026197434353 26.794170543889802 28.40719389123215 22.438373590534514 30-31 22.57574293434397 26.36631653773473 28.33779201202064 22.720148515900654 32-33 22.4099734247573 26.401450838328465 28.453007470192045 22.735568266722193 34-35 22.94725930371447 26.502497033372368 28.10480720330314 22.445436459610022 36-37 22.950227134514066 26.332107428887262 28.170183432881146 22.54748200371753 38-39 22.60831203637906 26.51015210103283 28.262004633624095 22.619531228964014 40-41 22.90642234335561 26.37085115269303 28.212444547271055 22.510281956680302 42-43 22.70598058735155 26.488912201291143 27.893939757604354 22.91116745375295 44-45 22.747379639237273 26.583179146633455 27.984139569911314 22.685301644217954 46-47 22.98268958588187 26.764180542295136 27.569238924153154 22.683890947669838 48-49 22.960684210444132 26.74134587307957 27.61960175947859 22.678368156997706 50-51 22.803365245185997 26.970244965461898 27.585400668942796 22.640989120409312 52-53 22.999166616247273 26.924501677501954 27.351810901908586 22.724520804342184 54-55 22.811908524044842 26.768166111573283 27.43851284540147 22.981412518980406 56-57 22.823108858653356 26.973699741857466 27.39391543749316 22.80927596199602 58-59 22.951917159290076 26.739185774212835 27.447627166088136 22.861269900408956 60-61 22.810342232250587 26.7834816919512 27.72025463658053 22.685921439217683 62-63 22.66730718836665 26.832428323260793 27.7672400857836 22.73302440258896 64-65 22.57164159877582 26.876168652970083 27.60704763614929 22.945142112104804 66-67 22.608743347955485 26.92787049274758 27.407936172384844 23.05544998691209 68-69 22.634077375781327 26.726668746429883 27.66070758542224 22.978546292366552 70-71 22.84092504022733 26.596692681934602 27.445356127291515 23.11702615054655 72-73 22.6662700373909 26.669532078954212 27.532434983639043 23.13176290001585 74-75 22.593601729671654 27.00620868732117 27.268720248331878 23.1314693346753 76-77 22.78359247383387 26.80774162322721 27.28328899939305 23.12537690354587 78-79 22.74036982178958 26.798216354351766 27.374664122541052 23.086749701317597 80-81 22.78715877057406 26.858623899772116 27.276617324502567 23.077600005151258 82-83 22.867681525793586 26.79275711722556 27.39518580055883 22.944375556422024 84-85 22.81862377758627 26.839006706882984 27.217108015786778 23.125261499743964 86-87 22.822803248640934 26.76941877929288 27.282600380319554 23.125177591746628 88-89 22.853213061242972 26.71907100896381 27.12961115988425 23.298104769908974 90-91 22.881384386300123 26.682910603170342 27.201130178193978 23.234574832335557 92-93 22.856037511267473 26.549719176897668 27.314384849645357 23.279858462189505 94-95 22.791863349114777 26.473825146489467 27.430120552953074 23.30419095144268 96-97 22.850249467100795 26.3187986538927 27.44672959952527 23.384222279481236 98-99 22.724845262424388 26.384771987765436 27.236233674722193 23.654149075087982 100-101 22.982655038173437 25.78218999732818 27.667959119029806 23.567195845468575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2280.0 1 1271.5 2 289.5 3 303.0 4 274.0 5 285.5 6 436.0 7 912.5 8 1463.5 9 1776.0 10 1818.5 11 1813.0 12 1976.0 13 2190.0 14 2423.5 15 2666.0 16 2869.5 17 3034.5 18 3215.5 19 3496.0 20 3874.0 21 4418.5 22 5178.5 23 6345.5 24 8082.0 25 10317.0 26 13092.5 27 16469.5 28 19509.0 29 22932.0 30 27375.0 31 32841.5 32 39171.0 33 43326.5 34 46424.5 35 50529.0 36 55846.5 37 61330.5 38 65429.0 39 68863.0 40 72291.0 41 76708.5 42 80701.5 43 83996.0 44 87866.5 45 91593.5 46 94368.0 47 98107.5 48 103259.0 49 102558.5 50 96978.5 51 93553.5 52 92206.0 53 91742.0 54 91194.5 55 86095.5 56 78544.5 57 71738.5 58 65325.5 59 57504.5 60 47649.0 61 39702.0 62 33129.5 63 26039.0 64 19330.0 65 14365.5 66 10523.0 67 8329.0 68 6430.5 69 4565.5 70 3484.5 71 2541.0 72 1896.5 73 1469.5 74 1101.5 75 787.0 76 567.5 77 364.0 78 247.5 79 155.5 80 105.0 81 90.0 82 68.5 83 49.5 84 36.0 85 25.5 86 19.0 87 16.0 88 13.5 89 11.0 90 11.5 91 10.5 92 8.5 93 8.5 94 6.5 95 7.5 96 7.5 97 5.5 98 4.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33571215217397216 2 0.11978899425020596 3 0.11306931331463994 4 0.12009973094086798 5 0.13742330144527518 6 0.08863764101133917 7 0.096483742450555 8 0.10258195000479699 9 0.09019132446464924 10-11 0.08898721978833393 12-13 0.09283258633527634 14-15 0.09452221709075104 16-17 0.0833939593564177 18-19 0.09828989946502795 20-21 0.10862189442953987 22-23 0.11666220630041944 24-25 0.1307618836392083 26-27 0.12973256835139035 28-29 0.12454714982596804 30-31 0.11460357572478362 32-33 0.10768968435755383 34-35 0.3294585762743991 36-37 0.489759866569665 38-39 0.4643183000217127 40-41 0.44359604696318977 42-43 0.47498045272005307 44-45 0.4827100279002706 46-47 0.489449129879003 48-49 0.49947038815285294 50-51 0.4884003935480187 52-53 0.49259533887195583 54-55 0.47952497682098494 56-57 0.4581618293379716 58-59 0.43870194408526303 60-61 0.3979371744790402 62-63 0.46736740379883374 64-65 0.49783902052687734 66-67 0.5050442275416028 68-69 0.518075747506241 70-71 0.510637487973519 72-73 0.5026554392321385 74-75 0.5094528043403701 76-77 0.4867690259220432 78-79 0.46697898293550616 80-81 0.46791119300749223 82-83 0.4815641863534544 84-85 0.4809815550584632 86-87 0.48439965865574536 88-89 0.49640186333256553 90-91 0.5112006982253439 92-93 0.5030050180091333 94-95 0.508054489232391 96-97 0.5075495421100653 98-99 0.5023058604551438 100-101 0.48946855092216934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2574527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.057332203399014 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53709632630581 45.20076723626193 2 13.382299250614905 15.806457849777242 3 4.519631017008724 8.00752051224811 4 1.9896525830636207 4.700142942693568 5 1.0360183660958977 3.0592240407674045 6 0.6241260101127987 2.2115530269608117 7 0.3857046463464806 1.5945081202175047 8 0.2729674240556979 1.2896582274530755 9 0.18802299412023327 0.9993722783072722 >10 0.9766835413519647 10.194946289186507 >50 0.05872438844005819 2.3602090761320187 >100 0.026700558238446248 2.9713854124749646 >500 0.0016478432258793844 0.6677129428617032 >1k 6.59137290336046E-4 0.711349986453343 >5k 6.59137290336046E-5 0.2251920582046946 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5785 0.22470146943496805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4866 0.18900559209516932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2899 0.11260320827864692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.884208633275161E-5 2 0.0 0.0 0.0 0.0 3.884208633275161E-5 3 0.0 0.0 0.0 0.0 3.884208633275161E-5 4 0.0 0.0 0.0 0.0 3.884208633275161E-5 5 0.0 0.0 0.0 0.0 3.884208633275161E-5 6 0.0 0.0 0.0 0.0 3.884208633275161E-5 7 0.0 0.0 0.0 0.0 3.884208633275161E-5 8 0.0 0.0 0.0 0.0 7.768417266550322E-5 9 0.0 0.0 0.0 0.0 1.1652625899825482E-4 10-11 0.0 0.0 0.0 5.826312949912741E-5 1.1652625899825482E-4 12-13 0.0 0.0 0.0 7.768417266550322E-5 1.1652625899825482E-4 14-15 0.0 0.0 0.0 7.768417266550322E-5 1.1652625899825482E-4 16-17 0.0 0.0 0.0 7.768417266550322E-5 1.5536834533100643E-4 18-19 0.0 0.0 0.0 7.768417266550322E-5 1.5536834533100643E-4 20-21 0.0 0.0 0.0 7.768417266550322E-5 1.5536834533100643E-4 22-23 0.0 0.0 0.0 1.3594730216463064E-4 2.524735611628855E-4 24-25 0.0 0.0 0.0 1.9421043166375804E-4 3.495787769947645E-4 26-27 0.0 0.0 0.0 5.437892086585226E-4 3.495787769947645E-4 28-29 0.0 0.0 0.0 0.0020197884893030835 3.495787769947645E-4 30-31 0.0 0.0 0.0 0.005962260252077372 3.689998201611403E-4 32-33 0.0 0.0 0.0 0.014216203597787087 3.884208633275161E-4 34-35 0.0 0.0 0.0 0.026296092447272837 3.884208633275161E-4 36-37 0.0 0.0 0.0 0.0483972395706085 3.884208633275161E-4 38-39 0.0 0.0 0.0 0.0830055384930902 3.884208633275161E-4 40-41 0.0 0.0 0.0 0.12015799407036709 3.884208633275161E-4 42-43 0.0 0.0 0.0 0.15820381763329733 3.884208633275161E-4 44-45 0.0 0.0 0.0 0.2047560581031001 3.884208633275161E-4 46-47 0.0 0.0 0.0 0.26548566008435726 4.466839928266435E-4 48-49 0.0 0.0 0.0 0.3324688379651874 5.049471223257709E-4 50-51 0.0 0.0 0.0 0.40232633023464115 5.049471223257709E-4 52-53 0.0 0.0 0.0 0.47509697897905134 5.437892086585226E-4 54-55 0.0 0.0 0.0 0.5573450967886528 5.826312949912742E-4 56-57 0.0 0.0 0.0 0.6562370485918384 6.214733813240257E-4 58-59 0.0 0.0 0.0 0.7692480987769792 6.603154676567773E-4 60-61 0.0 0.0 0.0 0.8947663007612661 6.603154676567773E-4 62-63 0.0 0.0 0.0 1.0217993441125302 6.99157553989529E-4 64-65 0.0 0.0 0.0 1.1608734342269473 6.99157553989529E-4 66-67 0.0 0.0 0.0 1.3256221434073132 6.99157553989529E-4 68-69 0.0 0.0 0.0 1.5186090493515896 6.99157553989529E-4 70-71 0.0 0.0 0.0 1.7233068443251907 6.99157553989529E-4 72-73 0.0 0.0 0.0 1.9382006869611388 8.545258993205354E-4 74-75 0.0 0.0 0.0 2.1670776806768774 8.545258993205354E-4 76-77 0.0 0.0 0.0 2.4199979258325897 8.545258993205354E-4 78-79 0.0 0.0 0.0 2.707701259299281 8.545258993205354E-4 80-81 0.0 0.0 0.0 3.019836265069273 9.127890288196628E-4 82-83 0.0 0.0 0.0 3.352324524077627 0.0010487363309842933 84-85 0.0 0.0 0.0 3.685822677330632 0.0010487363309842933 86-87 0.0 0.0 0.0 4.0414802408364725 0.0010681573741506693 88-89 0.0 0.0 0.0 4.437941416034867 0.0010875784173170451 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6320 0.0 31.701172 1 GTATCAA 9150 0.0 29.575607 1 ATCAACG 10710 0.0 24.775139 3 TCAACGC 10755 0.0 24.671476 4 CAACGCA 10835 0.0 24.533047 5 TATCAAC 11015 0.0 24.218178 2 AACGCAG 11175 0.0 23.904066 6 TACATGG 6855 0.0 22.53345 2 GTACATG 7185 0.0 22.334171 1 ACGCAGA 12215 0.0 21.67497 7 CGCAGAG 12370 0.0 21.364674 8 GAGTACT 6930 0.0 21.186344 12-13 ACATGGG 7330 0.0 20.426815 3 CGCGGAA 315 0.0 20.376911 82-83 AGAGTAC 10265 0.0 19.60814 10-11 GTACTTT 7365 0.0 19.42207 14-15 GCAGAGT 14075 0.0 18.338108 9 AGTACTT 7425 0.0 17.924103 12-13 CATGGGG 4975 0.0 17.333883 4 GTATAGT 785 0.0 16.93434 1 >>END_MODULE