FastQCFastQC Report
Fri 27 May 2016
SRR939223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574527
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.22369157519031652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.20675642554923682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.12211951943017106No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25820.10029026691116465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.027.3784581
GTATCAA101900.026.90831
GTACATG75350.023.8174951
TACATGG74850.023.263552
ATCAACG119050.022.6769493
TATCAAC121450.022.3069552
TCAACGC121250.022.265494
CAACGCA121800.022.1649485
AACGCAG125100.021.6181856
ACATGGG76950.021.1488723
GAGTACT74150.020.15663312-13
AGAGTAC109200.019.83520710-11
ACGCAGA138150.019.404367
CGCAGAG139700.019.0192498
GTACTTT78550.018.9671514-15
CATGGGG50850.018.7545594
AGTACTT79150.018.73344612-13
GTACCGT2101.976498E-518.0747556
GTGTAGC15750.017.530141
GCAGAGT155350.016.9508659
Produced by FastQC (version 0.11.5)