##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939223_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574527 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50107650842271 34.0 31.0 34.0 31.0 34.0 2 32.71511155252984 34.0 31.0 34.0 31.0 34.0 3 32.80848792807378 34.0 31.0 34.0 31.0 34.0 4 36.26355054734326 37.0 37.0 37.0 35.0 37.0 5 36.220441269405995 37.0 37.0 37.0 35.0 37.0 6 36.25198492771682 37.0 37.0 37.0 35.0 37.0 7 36.208093758581676 37.0 37.0 37.0 35.0 37.0 8 36.19244078621044 37.0 37.0 37.0 35.0 37.0 9 37.94394310100457 39.0 38.0 39.0 35.0 39.0 10-11 37.8831618390485 39.0 38.0 39.0 35.0 39.0 12-13 37.817916067689325 39.0 38.0 39.0 35.0 39.0 14-15 39.20151488020906 40.5 39.0 41.0 36.0 41.0 16-17 39.13685154593446 40.0 38.5 41.0 36.0 41.0 18-19 39.152065408519704 40.5 38.5 41.0 36.0 41.0 20-21 39.21902256220268 41.0 39.0 41.0 36.0 41.0 22-23 39.18143177368115 41.0 39.0 41.0 36.0 41.0 24-25 39.131022902459364 41.0 39.0 41.0 36.0 41.0 26-27 38.995492570091514 40.0 39.0 41.0 35.0 41.0 28-29 38.8517459712017 40.0 38.0 41.0 35.0 41.0 30-31 38.67240429018612 40.0 38.0 41.0 35.0 41.0 32-33 38.54609798227014 40.0 38.0 41.0 34.0 41.0 34-35 38.51018381240515 40.0 38.0 41.0 34.0 41.0 36-37 38.407879971738495 40.0 38.0 41.0 34.0 41.0 38-39 38.27944084486199 40.0 38.0 41.0 34.0 41.0 40-41 38.11960022171063 40.0 38.0 41.0 33.5 41.0 42-43 37.967739122565035 40.0 38.0 41.0 33.0 41.0 44-45 37.77393303702 40.0 37.5 41.0 33.0 41.0 46-47 37.52114815653516 40.0 37.0 41.0 33.0 41.0 48-49 37.2319410516961 40.0 37.0 41.0 32.0 41.0 50-51 36.98667852386089 40.0 36.5 41.0 31.0 41.0 52-53 36.75013973440558 39.5 36.0 41.0 31.0 41.0 54-55 36.50245792722313 39.0 35.5 41.0 31.0 41.0 56-57 36.224757790460146 39.0 35.0 40.5 30.0 41.0 58-59 35.91859494967425 38.5 35.0 40.0 29.5 41.0 60-61 35.55946160207293 38.0 35.0 40.0 28.5 41.0 62-63 35.16436961041776 38.0 34.0 40.0 28.0 41.0 64-65 34.75710742206238 37.0 34.0 40.0 28.0 41.0 66-67 34.396475935191205 36.5 34.0 39.0 27.0 41.0 68-69 33.92634841273757 36.0 33.5 39.0 26.0 40.0 70-71 33.485650956466955 35.5 33.0 39.0 26.0 40.0 72-73 33.01280138060312 35.0 33.0 38.0 25.5 40.0 74-75 32.51514724840719 35.0 32.0 37.0 24.5 39.0 76-77 30.528597486062488 33.5 29.5 35.5 20.5 37.5 78-79 31.511394520236145 34.5 31.0 36.0 22.0 38.5 80-81 31.542354770410256 35.0 32.0 36.0 22.5 37.5 82-83 31.24775366504216 35.0 31.5 35.5 21.5 37.0 84-85 30.86812315427261 34.0 31.0 35.0 20.0 37.0 86-87 30.30605544241719 34.0 31.0 35.0 17.0 36.0 88-89 29.76244257683062 34.0 30.0 35.0 8.5 36.0 90-91 29.305578461597023 34.0 29.5 35.0 4.5 35.0 92-93 29.016415442525947 34.0 29.0 35.0 2.0 35.0 94-95 28.675585845477634 34.0 29.0 35.0 2.0 35.0 96-97 28.110656442911647 34.0 29.0 35.0 2.0 35.0 98-99 27.325733425984655 33.5 28.0 35.0 2.0 35.0 100-101 23.678214095249338 29.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16623553433595362 1101 2 0.14844628885803957 1101 3 0.1793775145388281 1101 4 0.14536055664164138 1101 5 0.11794851033226195 1101 6 0.13263683590295017 1101 7 0.133596794071714 1101 8 0.13733173386286524 1101 9 0.19351029228425176 1101 10-11 0.22876404782132198 1101 12-13 0.1636333998055406 1101 14-15 0.1951274217380714 1101 16-17 0.22635030710038961 1101 18-19 0.22347340466870236 1101 20-21 0.19326127591342157 1101 22-23 0.29451931257767683 1101 24-25 0.125351815853314 1101 26-27 0.14697477016262184 1101 28-29 0.1410765123643003 1101 30-31 0.16390039665414946 1101 32-33 0.126408789977269 1101 34-35 0.20185863178394214 1101 36-37 0.31488254043684094 1101 38-39 0.4081229227131473 1101 40-41 0.2617454581594174 1101 42-43 0.2947683994662569 1101 44-45 0.381436679016133 1101 46-47 0.29210163664368594 1101 48-49 0.18444539924622916 1101 50-51 0.21112412678816384 1101 52-53 0.14405022671191858 1101 54-55 0.22914542395853488 1101 56-57 0.18208107659499007 1101 58-59 0.08589851504726198 1101 60-61 0.025818692854564063 1101 62-63 0.05629783196773985 1101 64-65 0.016875457796452054 1101 66-67 0.060412960307459684 1101 68-69 -0.06950972882560791 1101 70-71 0.004524970893342584 1101 72-73 -0.06934329712165521 1101 74-75 -0.10257947554581648 1101 76-77 0.013423997044565539 1101 78-79 0.10711149179682167 1101 80-81 0.07925872262349998 1101 82-83 0.11616407239072757 1101 84-85 0.2518319051635025 1101 86-87 0.26365015044592965 1101 88-89 0.3999531005654404 1101 90-91 0.505743114305865 1101 92-93 0.38400980687851316 1101 94-95 0.27223652731024117 1101 96-97 0.24825257029503334 1101 98-99 0.24909311612027452 1101 100-101 -0.29274414930998915 1102 1 0.049040618972128414 1102 2 0.07494307639373687 1102 3 0.11359834149349268 1102 4 0.05074609712048783 1102 5 0.1011139838263233 1102 6 0.12485291404058074 1102 7 0.09580560076979339 1102 8 0.09697852691928688 1102 9 0.2040905849920236 1102 10-11 0.18436330496070497 1102 12-13 0.11299712397626394 1102 14-15 0.16366049087245216 1102 16-17 0.18531292832005875 1102 18-19 0.13343348635552132 1102 20-21 0.1885977068203175 1102 22-23 0.24057468095915624 1102 24-25 0.24481500257413558 1102 26-27 0.19242464945275373 1102 28-29 0.11804514769116281 1102 30-31 0.24279153693723288 1102 32-33 0.27528062264861575 1102 34-35 0.30961868024694184 1102 36-37 0.44208150433130555 1102 38-39 0.42212755614207254 1102 40-41 0.43641607985258446 1102 42-43 0.3285112610711636 1102 44-45 0.31449327986184983 1102 46-47 0.3542426030985695 1102 48-49 0.3399569216429654 1102 50-51 0.32981272117214644 1102 52-53 0.2763949028941184 1102 54-55 0.24137516961730654 1102 56-57 0.3336534880967932 1102 58-59 0.29227460265467187 1102 60-61 0.4491331562551508 1102 62-63 0.4164516363431332 1102 64-65 0.3625221931588314 1102 66-67 0.4307250950568857 1102 68-69 0.28771898729281276 1102 70-71 0.09097721464472386 1102 72-73 0.16589095563598022 1102 74-75 0.2415967578882814 1102 76-77 0.3626424913691615 1102 78-79 0.313469375937224 1102 80-81 0.27400299375369386 1102 82-83 0.23268406686005605 1102 84-85 0.29012762297196204 1102 86-87 0.46631402392486976 1102 88-89 0.4269029853265529 1102 90-91 0.6044589243651188 1102 92-93 0.448402233838344 1102 94-95 0.34884586797668504 1102 96-97 0.3834322126516163 1102 98-99 0.29662933156974347 1102 100-101 0.045632240693823434 1103 1 0.0829141544250902 1103 2 0.08419518535306025 1103 3 0.1286823695719974 1103 4 0.057741386850565846 1103 5 0.11262155993216538 1103 6 0.08035606458649625 1103 7 0.1397253040533073 1103 8 0.10659627767944357 1103 9 0.1446171014590405 1103 10-11 0.18081080416781248 1103 12-13 0.13151766346499727 1103 14-15 0.19421108986441737 1103 16-17 0.12216651205396545 1103 18-19 0.08497629001109175 1103 20-21 0.2036180904332383 1103 22-23 0.2264716854778115 1103 24-25 0.1801666155508812 1103 26-27 0.10704130116280197 1103 28-29 0.1723867046491918 1103 30-31 0.23328272541408523 1103 32-33 0.22855457508389065 1103 34-35 0.26655725976427647 1103 36-37 0.21196648844819777 1103 38-39 0.26200030197871627 1103 40-41 0.3451013377855432 1103 42-43 0.337478949770734 1103 44-45 0.24515046160378517 1103 46-47 0.28093393304191494 1103 48-49 0.31132087179514656 1103 50-51 0.20952159071895693 1103 52-53 0.28282994748774826 1103 54-55 0.2568485009444075 1103 56-57 0.25060489694768506 1103 58-59 0.26303851780723164 1103 60-61 0.2894960320810398 1103 62-63 0.27271075565825953 1103 64-65 0.21156911004321444 1103 66-67 0.16948716287812715 1103 68-69 -1.6646734886904824E-4 1103 70-71 2.548779676203594E-4 1103 72-73 -0.013047439225495339 1103 74-75 -0.06107806737434629 1103 76-77 0.07819036108555721 1103 78-79 0.12835466717004707 1103 80-81 0.1584647238246717 1103 82-83 0.10592708364249148 1103 84-85 0.16344645296455695 1103 86-87 0.34562856820152277 1103 88-89 0.22448113474250775 1103 90-91 0.2878982094764879 1103 92-93 0.1754130609305271 1103 94-95 0.05325692198698562 1103 96-97 0.034859683650410034 1103 98-99 -0.09891484116616311 1103 100-101 -0.3325676079501747 1104 1 0.02863881736934104 1104 2 0.04462320787939689 1104 3 0.10996643715100873 1104 4 -0.06806226215029909 1104 5 0.06543707528759057 1104 6 0.08796820283607332 1104 7 0.08060658332490789 1104 8 0.08435862224827417 1104 9 0.15278870890460894 1104 10-11 0.0829383734742919 1104 12-13 0.0651281489470179 1104 14-15 0.07646514384867231 1104 16-17 0.10581139944994788 1104 18-19 0.11216090377812549 1104 20-21 0.08908766272428892 1104 22-23 0.16247268894382216 1104 24-25 0.13413934488621493 1104 26-27 0.11021448574705772 1104 28-29 0.08818889641080574 1104 30-31 0.13354373725461244 1104 32-33 0.06057713093396444 1104 34-35 0.007599665426482716 1104 36-37 0.13085028007404986 1104 38-39 0.10929278834246503 1104 40-41 0.16108144185825068 1104 42-43 0.004429409170015219 1104 44-45 0.016720738350123554 1104 46-47 0.14723066384555494 1104 48-49 -0.06974175075590239 1104 50-51 -0.07244645700470898 1104 52-53 -0.10104588466042941 1104 54-55 -0.10056146841667868 1104 56-57 -0.008748088824866329 1104 58-59 -0.013289276172336884 1104 60-61 0.0710145491648575 1104 62-63 0.015694517585949086 1104 64-65 -0.0039270614756929945 1104 66-67 0.0569503860331082 1104 68-69 -0.010762810231724984 1104 70-71 -0.07619290767432574 1104 72-73 -0.09284890421209013 1104 74-75 -0.03123334636187991 1104 76-77 0.06535263666610192 1104 78-79 0.22821752066585432 1104 80-81 0.1428190469370385 1104 82-83 0.03413241049756621 1104 84-85 -0.12547327159878208 1104 86-87 -0.051365737497757635 1104 88-89 0.1235271041847561 1104 90-91 0.036541501287722156 1104 92-93 0.19347987627248742 1104 94-95 0.21025074808853716 1104 96-97 0.272040313067091 1104 98-99 0.045849980709395055 1104 100-101 -0.19607990502038675 1105 1 0.022289203042276995 1105 2 0.08472034230670289 1105 3 0.06531458696506576 1105 4 -0.004085801452532678 1105 5 0.05615897042632412 1105 6 0.011311254966848594 1105 7 0.07123851934684211 1105 8 0.057087087615357746 1105 9 0.08183345553315036 1105 10-11 0.030948429618419482 1105 12-13 0.0544380072346371 1105 14-15 0.0188602517862293 1105 16-17 0.04184297081617672 1105 18-19 0.06413371256412859 1105 20-21 0.07699619357946119 1105 22-23 0.018284021115192672 1105 24-25 -0.04146888316014241 1105 26-27 -0.009597135578601979 1105 28-29 -0.0819417317233686 1105 30-31 0.03225352004082538 1105 32-33 0.02798917313978677 1105 34-35 0.12393688360143784 1105 36-37 0.05646506763934411 1105 38-39 0.10047518848382708 1105 40-41 0.09664394068690996 1105 42-43 0.21141489523036938 1105 44-45 0.1810216944334755 1105 46-47 0.05531922002850109 1105 48-49 0.12356566578425543 1105 50-51 0.1928095114881856 1105 52-53 0.19796295864203017 1105 54-55 0.09759785025010359 1105 56-57 -0.0076554603854646075 1105 58-59 0.08665316070233331 1105 60-61 0.19560578404114892 1105 62-63 0.1365227304784824 1105 64-65 -0.04474199468908324 1105 66-67 -0.012973014849883668 1105 68-69 -0.03436101426221683 1105 70-71 0.09186892850905792 1105 72-73 -0.15884596447666155 1105 74-75 -0.20500731872622424 1105 76-77 -0.0373324823289245 1105 78-79 -0.003882355372624602 1105 80-81 -0.1385968988518833 1105 82-83 -0.10011229436424074 1105 84-85 0.019270144116756427 1105 86-87 -0.08100512459772702 1105 88-89 0.08339998338519194 1105 90-91 0.12757423730323225 1105 92-93 0.07361149502789388 1105 94-95 -0.19391387709519847 1105 96-97 -0.34795417158229824 1105 98-99 -0.18969002834006332 1105 100-101 -0.6137306333947201 1106 1 0.049042976372952296 1106 2 0.07757139697413606 1106 3 0.1018778886114049 1106 4 0.05126889608786911 1106 5 0.08966825880951745 1106 6 0.09538413436906978 1106 7 0.09714406042233037 1106 8 0.07832169411993561 1106 9 0.08536335657728955 1106 10-11 0.10446237745042453 1106 12-13 0.04733843354505751 1106 14-15 0.04895226818583609 1106 16-17 0.051577670235445794 1106 18-19 0.04453925603963427 1106 20-21 0.07934623235676952 1106 22-23 0.07399203447570102 1106 24-25 0.10544029770257879 1106 26-27 0.14151394924667926 1106 28-29 0.18609614661256302 1106 30-31 0.2119655290758402 1106 32-33 0.27112642905015605 1106 34-35 0.08469362745929487 1106 36-37 0.13476112195363754 1106 38-39 0.3004330854438422 1106 40-41 0.27894796097864827 1106 42-43 0.3085364598725917 1106 44-45 0.30002826840487984 1106 46-47 0.45378720766090197 1106 48-49 0.44466314597903533 1106 50-51 0.363214280103179 1106 52-53 0.5315784646293693 1106 54-55 0.5641057905131674 1106 56-57 0.47642792763381436 1106 58-59 0.4602530117645429 1106 60-61 0.48127568180756697 1106 62-63 0.5830235838536098 1106 64-65 0.6254898017461485 1106 66-67 0.6322505872640463 1106 68-69 0.5298232359093831 1106 70-71 0.49837977964189406 1106 72-73 0.4426462762896364 1106 74-75 0.45933382355531194 1106 76-77 0.43637754635462755 1106 78-79 0.6024347634902938 1106 80-81 0.48696178928870637 1106 82-83 0.3878111960463464 1106 84-85 0.5077330368698902 1106 86-87 0.51722327342274 1106 88-89 0.731706570316657 1106 90-91 0.7964991423485337 1106 92-93 0.6747674771910148 1106 94-95 0.6232003572294786 1106 96-97 0.6246982239366794 1106 98-99 0.6301447892282859 1106 100-101 0.2878560820675844 1107 1 -0.08544294266223318 1107 2 -0.05145037631839244 1107 3 0.027888484717188078 1107 4 0.07657275978827016 1107 5 0.05594636689318122 1107 6 0.11070843676282038 1107 7 0.031085793553252472 1107 8 0.07136824371580985 1107 9 0.08627515915812722 1107 10-11 0.15473767513240944 1107 12-13 0.06643048965418785 1107 14-15 0.10713942571083379 1107 16-17 0.0527018471729761 1107 18-19 0.10247109257433351 1107 20-21 0.10058261062812335 1107 22-23 0.20455858516476866 1107 24-25 0.07913768075597005 1107 26-27 0.09677144685044681 1107 28-29 0.0757471303832844 1107 30-31 0.16486681819062454 1107 32-33 0.16262363003584568 1107 34-35 0.06318517250348776 1107 36-37 0.08587142257356817 1107 38-39 0.14611444354653713 1107 40-41 0.009221384625725193 1107 42-43 0.07123581196406548 1107 44-45 0.11851139330605065 1107 46-47 0.1143242455534974 1107 48-49 -0.011413265842747933 1107 50-51 0.09539492470612032 1107 52-53 0.13854974439350798 1107 54-55 0.017175449952866018 1107 56-57 -0.027244578907406947 1107 58-59 -0.11473880560033933 1107 60-61 -0.0076786555096148845 1107 62-63 -0.01820309563631639 1107 64-65 -0.047322327554446986 1107 66-67 0.0723595545862139 1107 68-69 0.12855876663542176 1107 70-71 0.15696266436727058 1107 72-73 0.07147324430285096 1107 74-75 0.032819661902884434 1107 76-77 0.008914654523533017 1107 78-79 0.31994116156263885 1107 80-81 0.25626377960488966 1107 82-83 0.3232568747365505 1107 84-85 0.44094703604089247 1107 86-87 0.5097657694420441 1107 88-89 0.4087569955208643 1107 90-91 0.5204227272732247 1107 92-93 0.5308458248648371 1107 94-95 0.3967757816040347 1107 96-97 0.5346555636139669 1107 98-99 0.5010229698006619 1107 100-101 -0.1718451720391876 1108 1 -0.021635050139444445 1108 2 -0.012461027088470189 1108 3 -0.04063800844888732 1108 4 0.02045314401216558 1108 5 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-0.6276427686411665 2316 90-91 -0.7141345313802034 2316 92-93 -0.6991866071293735 2316 94-95 -0.372482782936828 2316 96-97 -0.5511128553669131 2316 98-99 -0.5353523143898293 2316 100-101 -0.06414269856088595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 17.0 5 32.0 6 73.0 7 193.0 8 481.0 9 1035.0 10 1666.0 11 2787.0 12 3970.0 13 5857.0 14 7416.0 15 9754.0 16 12403.0 17 16844.0 18 17267.0 19 12938.0 20 12196.0 21 12943.0 22 13367.0 23 14535.0 24 17019.0 25 19905.0 26 23266.0 27 28807.0 28 34989.0 29 42325.0 30 52387.0 31 64260.0 32 80560.0 33 104752.0 34 146096.0 35 216082.0 36 355756.0 37 612497.0 38 571267.0 39 58769.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28659248383349 19.059003922213652 14.90339848529199 23.75100510866087 2 15.6597697363438 20.021386452734813 40.10165750835007 24.21718630257131 3 19.557106994799433 23.428070476635128 29.625752613975305 27.389069914590138 4 13.464026595953355 16.758612358697345 36.35961091105278 33.41775013429651 5 14.37599994096003 36.5945666912796 34.14949619871922 14.879937169041149 6 32.915094695064376 35.57550571425353 17.073932415546622 14.435467175135472 7 29.091168979777642 32.02467870797238 20.39345479771624 18.49069751453374 8 26.05344593395214 35.78832150527067 19.315431533637053 18.84280102714013 9 25.8245106771069 17.374220584985125 20.27121875202707 36.5300499858809 10-11 25.1905495650269 26.40316065824907 26.991831897665087 21.414457879058947 12-13 25.856477714158753 23.47295250739262 27.633483742838976 23.037086035609647 14-15 22.614174971669286 25.19809167013821 25.72891267341943 26.458820684773077 16-17 22.570095536224276 28.240935944642555 26.500032919416185 22.688935599716988 18-19 22.4050908001918 27.115238472006403 28.373236787424794 22.106433940377 20-21 22.977929538124865 26.18366402838269 28.632851782094342 22.205554651398103 22-23 22.63196307515905 26.081315130895888 28.602419007452628 22.68430278649243 24-25 22.63963144953124 26.283555546406056 28.605368343633963 22.471444660428734 26-27 22.50652166482294 26.519035182031747 28.51130862118255 22.463134531962762 28-29 22.300659499783844 26.858933699277575 28.45478023730184 22.385626563636738 30-31 22.53353689859991 26.430014599288658 28.402792762155503 22.633655739955923 32-33 22.436917538639136 26.435865694941246 28.442661506366022 22.684555260053592 34-35 22.90664070185784 26.570091096311998 28.17589642978052 22.347371772049645 36-37 22.87781730895878 26.392807146850718 28.256896382242907 22.472479161947597 38-39 22.557063131780623 26.521610668218642 28.365914242661646 22.555411957339093 40-41 22.861752972725398 26.405345758391206 28.279884684399104 22.453016584484295 42-43 22.63893715000708 26.52208984486152 28.011029238241765 22.82794376688964 44-45 22.694113004155444 26.58672479679186 28.075389966183124 22.643772232869573 46-47 22.906564381433288 26.848358904641678 27.63346280831229 22.61161390561274 48-49 22.912307830048537 26.81436847339777 27.657207979067095 22.616115717486597 50-51 22.800264126392246 26.965692700595255 27.643060370359578 22.590982802652917 52-53 23.01088395792153 26.97594855435356 27.355766485675208 22.657401002049703 54-55 22.778922108799016 26.81611029909572 27.466831771428307 22.938135820676962 56-57 22.79183710250465 27.04743434425042 27.402528697504437 22.75819985574049 58-59 22.931086654130446 26.83398545762189 27.39933895456313 22.835588933684534 60-61 22.75796298346512 26.903239966514587 27.66076010340856 22.678036946611737 62-63 22.680923346199666 26.918811584283674 27.692694934274492 22.707570135242168 64-65 22.485791810279615 26.99153053418288 27.548477199407184 22.974200456130326 66-67 22.513320439849334 26.985966197526672 27.404156342362786 23.09655702026121 68-69 22.603532689918453 26.8393074145625 27.584323661782044 22.972836233737002 70-71 22.745774805580755 26.702172224678982 27.402223282611388 23.149829687128875 72-73 22.628175971741605 26.78427920934603 27.408685168188175 23.17885965072419 74-75 22.531206703211893 27.18784848634332 27.123623096592116 23.15732171385268 76-77 22.6409325055364 26.871778542391766 27.25073348315062 23.236555468921217 78-79 22.59667569423068 26.913650977996888 27.35806348555897 23.13160984221346 80-81 22.7007516168502 26.94697896638117 27.23888597564528 23.113383441123347 82-83 22.72523527695853 26.93285619566218 27.28620375301173 23.055704774367562 84-85 22.66913935436283 26.947188152850632 27.14139862223209 23.24227387055445 86-87 22.66752422228448 26.85176309922363 27.206243616696746 23.274469061795138 88-89 22.635877688872664 26.908685926256144 27.00361685269657 23.45181953217462 90-91 22.66283776287561 26.83406291914788 27.12197163067743 23.381127687299085 92-93 22.560163478572957 26.710887087220293 27.196859850372512 23.532089583834235 94-95 22.429906542056074 26.694806463478532 27.267105763505295 23.608181230960096 96-97 22.486169304109065 26.503179030555906 27.318435580594024 23.692216084741002 98-99 22.324501120898784 26.631751101623173 27.03981147381634 24.003936303661707 100-101 22.479533472117105 25.956119391332365 27.612085957377886 23.952261179172645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 161.0 2 117.0 3 167.5 4 166.0 5 165.5 6 319.5 7 684.0 8 1121.5 9 1475.5 10 1479.0 11 1384.5 12 1455.0 13 1643.5 14 1818.0 15 1982.5 16 2191.5 17 2362.5 18 2571.0 19 2878.0 20 3372.0 21 4101.0 22 5090.5 23 6504.0 24 8293.5 25 10529.0 26 13464.0 27 16760.5 28 19749.5 29 23293.5 30 27787.0 31 33114.0 32 39524.0 33 43944.0 34 47080.5 35 51292.5 36 56160.0 37 61356.0 38 65916.5 39 69195.0 40 72369.5 41 76959.5 42 81082.5 43 84647.0 44 88632.0 45 92308.0 46 94595.0 47 98300.0 48 104179.5 49 103952.0 50 97843.5 51 94130.0 52 92555.0 53 91697.5 54 91477.5 55 86144.5 56 78541.5 57 72188.5 58 65719.0 59 57575.5 60 47753.5 61 39403.0 62 32775.5 63 26020.5 64 19100.0 65 13890.0 66 10030.5 67 7995.0 68 6201.0 69 4343.0 70 3219.0 71 2186.0 72 1570.0 73 1316.5 74 1037.0 75 746.5 76 502.5 77 285.5 78 169.5 79 113.5 80 73.5 81 41.5 82 25.5 83 15.0 84 8.0 85 5.5 86 4.0 87 3.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3450730949801653 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.516311151524144E-4 16-17 0.0022722620504659692 18-19 1.9421043166375804E-5 20-21 0.0 22-23 0.0 24-25 3.3015773382838866E-4 26-27 0.0012041046763152999 28-29 0.0 30-31 0.003359840467783014 32-33 0.0 34-35 0.010623310612007565 36-37 0.02913156474956371 38-39 0.023363514929150092 40-41 0.014526940288449102 42-43 0.010817521043671324 44-45 0.0028354723022908676 46-47 0.0010487363309842933 48-49 6.020523381576499E-4 50-51 7.768417266550322E-5 52-53 1.5536834533100643E-4 54-55 0.0 56-57 0.0 58-59 0.004661050359930193 60-61 0.010565047482508437 62-63 0.004194945323937173 64-65 0.0016896307554746949 66-67 0.0038842086332751607 68-69 0.003359840467783014 70-71 1.9421043166375804E-5 72-73 0.0 74-75 0.0 76-77 0.21829252519006406 78-79 1.7478938849738225E-4 80-81 0.002990840647621874 82-83 3.1073669066201287E-4 84-85 1.9421043166375804E-5 86-87 0.11172926133616 88-89 0.41007532645802514 90-91 0.3951211232199157 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001184683633148924 100-101 7.185785971559048E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2574527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.36578802344139 #Duplication Level Percentage of deduplicated Percentage of total 1 74.93074929971735 42.98461480765215 2 14.415336763465728 16.538943061189933 3 4.6992259130516825 8.087243928071569 4 2.140375645568701 4.911373422969224 5 1.0819927322929617 3.10346828618111 6 0.6486715171019627 2.2326931648149193 7 0.4227215502991937 1.6974828393182848 8 0.29407064451330367 1.3495675405653573 9 0.2244760431882708 1.1589520598891296 >10 1.046366570341857 10.647661741059192 >50 0.06480388235378597 2.535807314509049 >100 0.028428864519785588 3.0007353811231803 >500 0.0019667471702018097 0.7339006757882284 >1k 6.781886793625897E-4 0.5864124727486552 >5k 1.3563773587251793E-4 0.43114330411998913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5759 0.22369157519031652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5323 0.20675642554923682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3144 0.12211951943017106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2582 0.10029026691116465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.884208633275161E-5 5 0.0 0.0 0.0 0.0 3.884208633275161E-5 6 0.0 0.0 0.0 0.0 3.884208633275161E-5 7 0.0 0.0 0.0 0.0 3.884208633275161E-5 8 0.0 0.0 0.0 0.0 3.884208633275161E-5 9 0.0 0.0 0.0 3.884208633275161E-5 3.884208633275161E-5 10-11 0.0 0.0 0.0 9.710521583187902E-5 3.884208633275161E-5 12-13 0.0 0.0 0.0 1.1652625899825482E-4 3.884208633275161E-5 14-15 0.0 0.0 0.0 1.1652625899825482E-4 5.826312949912741E-5 16-17 0.0 0.0 0.0 1.1652625899825482E-4 7.768417266550322E-5 18-19 0.0 0.0 0.0 1.1652625899825482E-4 1.5536834533100643E-4 20-21 0.0 0.0 0.0 1.1652625899825482E-4 1.5536834533100643E-4 22-23 0.0 0.0 0.0 1.7478938849738222E-4 2.3305251799650965E-4 24-25 0.0 0.0 0.0 2.718946043292613E-4 2.913156474956371E-4 26-27 0.0 0.0 0.0 6.214733813240257E-4 3.495787769947645E-4 28-29 0.0 0.0 0.0 0.0021363147483013387 3.495787769947645E-4 30-31 0.0 0.0 0.0 0.006117628597408378 3.689998201611403E-4 32-33 0.0 0.0 0.0 0.014371571943118094 4.272629496602677E-4 34-35 0.0 0.0 0.0 0.026820460612764985 4.272629496602677E-4 36-37 0.0 0.0 0.0 0.049135239210930784 4.272629496602677E-4 38-39 0.0 0.0 0.0 0.08401543273774173 4.466839928266435E-4 40-41 0.0 0.0 0.0 0.12151746709201341 4.661050359930193E-4 42-43 0.0 0.0 0.0 0.15995171151827112 4.661050359930193E-4 44-45 0.0 0.0 0.0 0.20718368849889707 5.04947122325771E-4 46-47 0.0 0.0 0.0 0.2683599744729809 5.826312949912742E-4 48-49 0.0 0.0 0.0 0.3360034678214678 5.826312949912742E-4 50-51 0.0 0.0 0.0 0.40640474929958004 5.826312949912742E-4 52-53 0.0 0.0 0.0 0.47993281872747884 6.020523381576499E-4 54-55 0.0 0.0 0.0 0.5630354624364009 6.214733813240257E-4 56-57 0.0 0.0 0.0 0.6632092030885673 6.214733813240257E-4 58-59 0.0 1.9421043166375804E-5 0.0 0.7775408842090217 6.214733813240257E-4 60-61 0.0 3.884208633275161E-5 0.0 0.9034669280998024 6.214733813240257E-4 62-63 0.0 3.884208633275161E-5 0.0 1.0311214448323907 6.214733813240257E-4 64-65 0.0 3.884208633275161E-5 0.0 1.1711665871051264 6.214733813240257E-4 66-67 0.0 3.884208633275161E-5 0.0 1.3364008223646517 6.214733813240257E-4 68-69 0.0 3.884208633275161E-5 0.0 1.5295625176974257 6.214733813240257E-4 70-71 0.0 3.884208633275161E-5 0.0 1.7354838383905082 6.603154676567773E-4 72-73 0.0 3.884208633275161E-5 0.0 1.9520090486524322 7.379996403222806E-4 74-75 0.0 3.884208633275161E-5 0.0 2.1824203047783146 7.379996403222806E-4 76-77 0.0 3.884208633275161E-5 0.0 2.437671075113992 7.768417266550322E-4 78-79 0.0 3.884208633275161E-5 0.0 2.726830986818161 7.768417266550322E-4 80-81 0.0 3.884208633275161E-5 0.0 3.040810991688959 7.96262769821408E-4 82-83 0.0 3.884208633275161E-5 0.0 3.3749888814527873 8.739469424869112E-4 84-85 0.0 3.884208633275161E-5 0.0 3.709768823554773 8.93367985653287E-4 86-87 0.0 3.884208633275161E-5 0.0 4.066242070873601 8.93367985653287E-4 88-89 0.0 3.884208633275161E-5 0.0 4.463052824848992 8.93367985653287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7320 0.0 27.378458 1 GTATCAA 10190 0.0 26.9083 1 GTACATG 7535 0.0 23.817495 1 TACATGG 7485 0.0 23.26355 2 ATCAACG 11905 0.0 22.676949 3 TATCAAC 12145 0.0 22.306955 2 TCAACGC 12125 0.0 22.26549 4 CAACGCA 12180 0.0 22.164948 5 AACGCAG 12510 0.0 21.618185 6 ACATGGG 7695 0.0 21.148872 3 GAGTACT 7415 0.0 20.156633 12-13 AGAGTAC 10920 0.0 19.835207 10-11 ACGCAGA 13815 0.0 19.40436 7 CGCAGAG 13970 0.0 19.019249 8 GTACTTT 7855 0.0 18.96715 14-15 CATGGGG 5085 0.0 18.754559 4 AGTACTT 7915 0.0 18.733446 12-13 GTACCGT 210 1.976498E-5 18.074755 6 GTGTAGC 1575 0.0 17.53014 1 GCAGAGT 15535 0.0 16.950865 9 >>END_MODULE