FastQCFastQC Report
Fri 27 May 2016
SRR939222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531809
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.21869738199050562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.1863884676924681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.11462160060257309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65750.031.5617771
GTATCAA95350.029.6327551
ATCAACG112800.024.437383
TCAACGC114000.024.3047854
TATCAAC115650.024.0399322
CAACGCA115750.023.8145715
AACGCAG117650.023.4193256
GAGTACT68400.020.86810912-13
ACGCAGA131750.020.7692367
CGCAGAG132350.020.6389038
GTACATG76250.020.3026871
AGAGTAC105350.020.01945110-11
TACATGG74200.019.915512
GTACTTT71850.019.66900314-15
AGTACTT73600.018.87913712-13
ACTGCGT2552.7741953E-718.5649838
GCAGAGT146950.018.2017699
ACATGGG77150.018.1717413
TAGCCCG1600.001214828117.7611895
GTATAGC9900.017.2680421
Produced by FastQC (version 0.11.5)