##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2531809 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.072985758404364 33.0 31.0 34.0 30.0 34.0 2 32.185095321171545 34.0 31.0 34.0 30.0 34.0 3 32.250349453691015 34.0 31.0 34.0 30.0 34.0 4 35.68068602331377 37.0 35.0 37.0 35.0 37.0 5 35.712112564573395 37.0 35.0 37.0 35.0 37.0 6 35.78952361730288 37.0 36.0 37.0 35.0 37.0 7 35.72000336518276 37.0 36.0 37.0 35.0 37.0 8 35.713305387570706 37.0 36.0 37.0 35.0 37.0 9 37.38848349144821 39.0 38.0 39.0 35.0 39.0 10-11 37.290397893363995 39.0 37.5 39.0 34.0 39.0 12-13 37.19053866227666 39.0 37.0 39.0 34.0 39.0 14-15 38.49006994603464 40.0 38.0 41.0 34.0 41.0 16-17 38.389760049040035 40.0 38.0 41.0 34.0 41.0 18-19 38.26275718271007 40.0 38.0 41.0 33.5 41.0 20-21 38.24298535158063 40.0 38.0 41.0 33.5 41.0 22-23 38.28879666673118 40.0 38.0 41.0 34.0 41.0 24-25 38.241927412375894 40.0 38.0 41.0 34.0 41.0 26-27 38.12914343064583 40.0 38.0 41.0 33.0 41.0 28-29 37.98503362615426 40.0 38.0 41.0 33.0 41.0 30-31 37.78197703697238 40.0 38.0 41.0 33.0 41.0 32-33 37.58864610245085 40.0 37.5 41.0 32.5 41.0 34-35 37.28595660257152 40.0 37.0 41.0 31.5 41.0 36-37 37.02375751883337 40.0 37.0 41.0 30.5 41.0 38-39 36.833943437281405 40.0 37.0 41.0 30.0 41.0 40-41 36.64997102862025 40.0 36.0 41.0 30.0 41.0 42-43 36.449689530292375 40.0 36.0 41.0 30.0 41.0 44-45 36.19907346881222 39.0 36.0 41.0 29.5 41.0 46-47 36.100365193424935 39.5 36.0 41.0 29.0 41.0 48-49 35.9947545411206 40.0 36.0 41.0 28.0 41.0 50-51 34.88888123077215 38.5 34.0 40.0 26.0 40.5 52-53 35.03291895241703 38.5 34.5 39.5 26.5 40.5 54-55 35.55998576511894 39.0 35.0 41.0 27.0 41.0 56-57 35.466975391903574 39.0 35.0 41.0 27.0 41.0 58-59 35.2597755201913 39.0 35.0 41.0 26.0 41.0 60-61 34.96185790476296 38.5 35.0 40.5 26.0 41.0 62-63 34.57971118674434 38.0 34.0 40.0 25.5 41.0 64-65 34.212716085613096 37.5 34.0 40.0 24.5 41.0 66-67 33.82379002523493 37.0 34.0 40.0 23.0 41.0 68-69 33.4135248749017 36.0 33.5 39.0 22.0 41.0 70-71 32.979321899874755 36.0 33.0 39.0 21.5 41.0 72-73 32.53432308677313 35.0 33.0 38.5 20.0 40.0 74-75 32.08595652357662 35.0 32.0 37.5 18.5 39.5 76-77 31.642285417264887 35.0 32.0 37.0 17.5 39.0 78-79 31.205251462491837 35.0 31.5 36.5 14.0 39.0 80-81 30.75948383152126 35.0 31.0 36.0 8.5 37.5 82-83 30.353239719109933 35.0 31.0 35.5 6.5 37.0 84-85 29.9660819595791 34.0 30.5 35.0 2.0 37.0 86-87 29.55055732877164 34.0 30.0 35.0 2.0 36.0 88-89 29.12490535423486 34.0 29.5 35.0 2.0 36.0 90-91 28.70993665003956 34.0 29.0 35.0 2.0 36.0 92-93 28.29360350642564 34.0 29.0 35.0 2.0 35.0 94-95 27.802462784514944 34.0 28.0 35.0 2.0 35.0 96-97 27.19014961239177 34.0 26.5 35.0 2.0 35.0 98-99 26.46000290701234 33.5 25.0 35.0 2.0 35.0 100-101 23.462529163929823 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22230468222698363 1101 2 0.3241696391921778 1101 3 0.22188783153399072 1101 4 0.2798157539424082 1101 5 0.14559993342523114 1101 6 0.12857067283091084 1101 7 0.14320025698764027 1101 8 0.2570318691491664 1101 9 0.33713492888694674 1101 10-11 0.2103180172073067 1101 12-13 0.24811842290500152 1101 14-15 0.412095029023142 1101 16-17 0.45460658983110136 1101 18-19 0.4237733603158631 1101 20-21 0.4995142278752809 1101 22-23 0.568787191151614 1101 24-25 0.5488979379446874 1101 26-27 0.48853233368271987 1101 28-29 0.40680199227444547 1101 30-31 0.44468639106049324 1101 32-33 0.39481932810850395 1101 34-35 0.3651776388948704 1101 36-37 0.4235914920317754 1101 38-39 0.29850504331611916 1101 40-41 0.35115183262976757 1101 42-43 0.3037692607755318 1101 44-45 0.4778916999238234 1101 46-47 0.534861166303152 1101 48-49 0.5735095344068597 1101 50-51 0.49170139734776797 1101 52-53 0.5219462645380517 1101 54-55 0.5844376055436769 1101 56-57 0.5906482335253642 1101 58-59 0.530022415872935 1101 60-61 0.4040299227761821 1101 62-63 0.3484550634129491 1101 64-65 0.26307862695552586 1101 66-67 0.37068087301351227 1101 68-69 0.3554703731414435 1101 70-71 0.33690089313132177 1101 72-73 0.2769081701324936 1101 74-75 0.2285164897287686 1101 76-77 0.21143685897563458 1101 78-79 0.3324930623570097 1101 80-81 0.10376207024638617 1101 82-83 0.06259898527247643 1101 84-85 -0.07532920394360687 1101 86-87 -0.08962649194461036 1101 88-89 -0.16164570661765154 1101 90-91 -0.18812655088003183 1101 92-93 -0.2762864125572655 1101 94-95 -0.30730415296699576 1101 96-97 -0.5089270765348566 1101 98-99 -0.7091424301208953 1101 100-101 -0.5778214378097211 1102 1 0.2913152012471869 1102 2 0.3201060267368234 1102 3 0.34675076358602297 1102 4 0.08589559707766625 1102 5 0.22445703434248543 1102 6 0.24865355893195584 1102 7 0.2833518297947819 1102 8 0.31611886681950807 1102 9 0.3424896228920744 1102 10-11 0.3838665252387159 1102 12-13 0.32558540097142696 1102 14-15 0.41269136190950206 1102 16-17 0.4975044006847327 1102 18-19 0.4327052176071504 1102 20-21 0.5540981824755846 1102 22-23 0.6294891875531761 1102 24-25 0.6265272394908408 1102 26-27 0.5633957765793625 1102 28-29 0.5000605323973275 1102 30-31 0.554995673065271 1102 32-33 0.5459766830510446 1102 34-35 0.4044467849858009 1102 36-37 0.4450785544229987 1102 38-39 0.4476897040496155 1102 40-41 0.37969970575431944 1102 42-43 0.34792416637457535 1102 44-45 0.33325011579678687 1102 46-47 0.3285026730737215 1102 48-49 0.2185474922461239 1102 50-51 0.059590351824006405 1102 52-53 0.09522150198175439 1102 54-55 0.1393675791736726 1102 56-57 0.06329886439760912 1102 58-59 0.004064629152018995 1102 60-61 -0.12477973214515714 1102 62-63 -0.08060623981627657 1102 64-65 -0.1947481274985634 1102 66-67 -0.18378600434353842 1102 68-69 -0.26957927782657976 1102 70-71 -0.42900738127938354 1102 72-73 -0.4272812143498044 1102 74-75 -0.47375691472107917 1102 76-77 -0.6077758606797268 1102 78-79 -0.4921066700438246 1102 80-81 -0.45759266045614666 1102 82-83 -0.7023773626222898 1102 84-85 -0.70130087729747 1102 86-87 -0.8276635016979874 1102 88-89 -0.6527609721348675 1102 90-91 -0.6642988819098399 1102 92-93 -0.8197536587649736 1102 94-95 -1.072897267532344 1102 96-97 -1.2505082010560464 1102 98-99 -1.2946440555450245 1102 100-101 -1.233091091826136 1103 1 0.14799027369239326 1103 2 0.12085736307265904 1103 3 0.2006575194128999 1103 4 0.17845673717363297 1103 5 0.2469546381365646 1103 6 0.15689965817299623 1103 7 0.22789666247709306 1103 8 0.2646956272304237 1103 9 0.2642855932004622 1103 10-11 0.26642732991668083 1103 12-13 0.24220566814711475 1103 14-15 0.37645341027678114 1103 16-17 0.44930686590544155 1103 18-19 0.38390972399889733 1103 20-21 0.3443952510238404 1103 22-23 0.3902003550416211 1103 24-25 0.32274941631305154 1103 26-27 0.27735414612259746 1103 28-29 0.2516397921806046 1103 30-31 0.4168092477369427 1103 32-33 0.3625852614174008 1103 34-35 0.19062319249631088 1103 36-37 0.3725727684623834 1103 38-39 0.0864022274399403 1103 40-41 0.10440984869320857 1103 42-43 0.009877803474651614 1103 44-45 0.15322334334131682 1103 46-47 0.16677244469471475 1103 48-49 0.15625914863171886 1103 50-51 0.10898268620120177 1103 52-53 0.13708990225130435 1103 54-55 0.11153642249992402 1103 56-57 0.28121211716963757 1103 58-59 0.3310707591702027 1103 60-61 0.3285578294878988 1103 62-63 0.1174121512546975 1103 64-65 0.26310111125501834 1103 66-67 0.08145228982211705 1103 68-69 0.01379618604127586 1103 70-71 -0.009525591936117905 1103 72-73 0.07024624672900615 1103 74-75 0.08160962092927804 1103 76-77 0.14495561077874086 1103 78-79 0.15982955581243274 1103 80-81 0.13464800298034163 1103 82-83 0.07470139047651614 1103 84-85 0.04377259449234927 1103 86-87 -0.11290266998329912 1103 88-89 -0.1142571194016675 1103 90-91 -0.10486636852106912 1103 92-93 -0.15579828765544335 1103 94-95 -0.24486658796529426 1103 96-97 -0.27224460925772576 1103 98-99 -0.31999433866831595 1103 100-101 -0.47190149628943345 1104 1 0.19408686812085563 1104 2 0.29508189932575135 1104 3 0.2851598277175924 1104 4 0.2968522028249012 1104 5 0.25075865359529104 1104 6 0.23516134718649084 1104 7 0.313996084002099 1104 8 0.23124400739244777 1104 9 0.2146044759476169 1104 10-11 0.2705921928368582 1104 12-13 0.23113493478059866 1104 14-15 0.2591621810493123 1104 16-17 0.2542956115089652 1104 18-19 0.33874607142943347 1104 20-21 0.29683219842708297 1104 22-23 0.30005906892865397 1104 24-25 0.38184319444290793 1104 26-27 0.39268574589603844 1104 28-29 0.3680729779748404 1104 30-31 0.472670613990239 1104 32-33 0.47395558848695174 1104 34-35 0.45237974218204613 1104 36-37 0.4617921069806883 1104 38-39 0.2654471596239816 1104 40-41 0.22969479155739236 1104 42-43 0.36564836888414476 1104 44-45 0.28423394200845564 1104 46-47 0.3597543738543507 1104 48-49 0.40497178899264696 1104 50-51 0.23433889545739106 1104 52-53 0.3130404398413873 1104 54-55 0.2506805190217065 1104 56-57 0.3317708123075178 1104 58-59 0.3516884404994869 1104 60-61 0.3028889708397813 1104 62-63 0.29290053709054575 1104 64-65 0.31450332169112727 1104 66-67 0.31026442827523937 1104 68-69 0.3023284812019398 1104 70-71 0.23607095979139814 1104 72-73 0.30905776675476204 1104 74-75 0.36596959277611063 1104 76-77 0.25256894250220085 1104 78-79 0.25206701621590355 1104 80-81 0.21584918525641328 1104 82-83 0.2236884947934321 1104 84-85 0.17353662027782235 1104 86-87 0.1309258666114026 1104 88-89 -0.06005549441013258 1104 90-91 0.11023935801820173 1104 92-93 -0.06152198019691468 1104 94-95 -0.12349236382169337 1104 96-97 -0.08021044458131499 1104 98-99 -0.10608502367969663 1104 100-101 -0.2473106645242531 1105 1 0.043306720253696085 1105 2 0.04706649924931838 1105 3 0.04460931273035129 1105 4 0.024420509996033957 1105 5 0.0927048005093738 1105 6 0.15078289408264567 1105 7 0.1502608105611003 1105 8 0.0640576825093433 1105 9 0.12541859457161308 1105 10-11 0.2633213185144996 1105 12-13 0.14955935220570638 1105 14-15 0.06981993982863344 1105 16-17 0.12494950139652872 1105 18-19 0.18809822078751637 1105 20-21 0.13318647567564312 1105 22-23 0.09823672655060989 1105 24-25 0.19999990343147545 1105 26-27 0.11445741522846475 1105 28-29 0.15320001445093823 1105 30-31 0.20524253055429398 1105 32-33 0.1660766376171594 1105 34-35 0.15170712119660834 1105 36-37 0.17848458198623263 1105 38-39 0.12065350838098254 1105 40-41 0.06868350099890819 1105 42-43 -0.01761876865496248 1105 44-45 -0.0709449470534338 1105 46-47 0.048048334136872484 1105 48-49 0.14731926265351802 1105 50-51 0.14931320507253076 1105 52-53 0.20356316486225978 1105 54-55 0.18499532413808595 1105 56-57 0.16502535235762394 1105 58-59 0.24772660098300747 1105 60-61 0.1807480582531511 1105 62-63 0.20553945153613995 1105 64-65 0.16085387159275655 1105 66-67 0.0870696673208542 1105 68-69 0.2014096665926246 1105 70-71 0.09452434672304122 1105 72-73 0.05422113306804022 1105 74-75 0.061287761941038355 1105 76-77 0.026847795234253624 1105 78-79 0.05883078262920449 1105 80-81 0.11414139981948423 1105 82-83 0.06404396101848064 1105 84-85 -0.03023620972212626 1105 86-87 -0.226464046904713 1105 88-89 -0.22909294245139122 1105 90-91 -0.07293737956242197 1105 92-93 -0.20637489153795485 1105 94-95 -0.11430874716281281 1105 96-97 -0.11143202356096893 1105 98-99 -0.11691575022925704 1105 100-101 -0.2763411296877152 1106 1 0.22322409145400712 1106 2 0.2774432537432858 1106 3 0.2783180325311676 1106 4 0.31140127554365904 1106 5 0.2829474884078138 1106 6 0.2747102245157649 1106 7 0.23528092055042293 1106 8 0.25890241150329985 1106 9 0.391391945415684 1106 10-11 0.33254154341805986 1106 12-13 0.37199772261256214 1106 14-15 0.31696322692266676 1106 16-17 0.43432431329288335 1106 18-19 0.4390591225905851 1106 20-21 0.4406706749697449 1106 22-23 0.4352536079974314 1106 24-25 0.38810710198683296 1106 26-27 0.4659231188044828 1106 28-29 0.35008802780287596 1106 30-31 0.3354748214514416 1106 32-33 0.28793605921556065 1106 34-35 0.43211068900968286 1106 36-37 0.46083320985566445 1106 38-39 0.40888601816237014 1106 40-41 0.6528956545461995 1106 42-43 0.6432731846132569 1106 44-45 0.6065688016127382 1106 46-47 0.465042506344048 1106 48-49 0.5355326200641102 1106 50-51 0.5246033634899945 1106 52-53 0.5276407046034279 1106 54-55 0.4258913814857479 1106 56-57 0.4831982161842703 1106 58-59 0.4918386149915932 1106 60-61 0.6608172803932888 1106 62-63 0.5005650336314034 1106 64-65 0.41111188138782495 1106 66-67 0.4053634646887687 1106 68-69 0.5133191579603746 1106 70-71 0.37923692435854406 1106 72-73 0.4741606662974007 1106 74-75 0.44411766152789767 1106 76-77 0.41699225883106905 1106 78-79 0.31884555731524244 1106 80-81 0.2959034179321556 1106 82-83 0.4160935382982949 1106 84-85 0.4533797578830061 1106 86-87 0.39392885030177993 1106 88-89 0.4265723288282608 1106 90-91 0.34642895056972733 1106 92-93 0.32381416972226873 1106 94-95 0.35124489315566265 1106 96-97 0.5439125823106039 1106 98-99 0.31148666844156736 1106 100-101 0.21366242718114492 1107 1 0.037727165164675114 1107 2 0.08121832882122959 1107 3 0.11936350816281305 1107 4 0.10785114237252458 1107 5 0.13200446272154664 1107 6 0.13395486980498816 1107 7 0.1692792072128242 1107 8 0.1212372214491495 1107 9 0.13580900193565526 1107 10-11 0.15207661747692214 1107 12-13 0.12838638061487728 1107 14-15 0.1484346060108166 1107 16-17 0.140239043431599 1107 18-19 0.21500228097027474 1107 20-21 0.26545961279596497 1107 22-23 0.254794520567323 1107 24-25 0.22883756340966954 1107 26-27 0.2853963577237906 1107 28-29 0.37718402672434337 1107 30-31 0.3076283084756213 1107 32-33 0.1962571051370361 1107 34-35 0.3327325965892385 1107 36-37 0.314684132499643 1107 38-39 0.37106706295861613 1107 40-41 0.2945997672995233 1107 42-43 0.4120166627310482 1107 44-45 0.45074345377017977 1107 46-47 0.3614906746969808 1107 48-49 0.24540665684248353 1107 50-51 0.23955557793107118 1107 52-53 0.39949732988036146 1107 54-55 0.4162612883137342 1107 56-57 0.3790253792729885 1107 58-59 0.35138978210995475 1107 60-61 0.19677481212558945 1107 62-63 0.33064233551746014 1107 64-65 0.2896740365839392 1107 66-67 0.22242380623105618 1107 68-69 0.24544508721248803 1107 70-71 0.20843393801322918 1107 72-73 0.2664643941336138 1107 74-75 0.1782942350862342 1107 76-77 0.16110863203293846 1107 78-79 0.24077245703403705 1107 80-81 0.16396793029440815 1107 82-83 0.21370792269100036 1107 84-85 0.21781229176494676 1107 86-87 0.22274423764452322 1107 88-89 0.14373707356325127 1107 90-91 0.16835967185938117 1107 92-93 0.037166632140838374 1107 94-95 0.049745245058648635 1107 96-97 0.01077611295376002 1107 98-99 -0.06972988617876652 1107 100-101 0.10357624014285349 1108 1 0.05209473054612346 1108 2 0.07477818868818531 1108 3 0.12885866287873426 1108 4 0.21265856828761542 1108 5 0.12356137727076799 1108 6 0.18573382097050484 1108 7 0.13863756231120306 1108 8 0.15333383766716935 1108 9 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2316 60-61 -1.2274925404275265 2316 62-63 -1.1968348792530605 2316 64-65 -1.5349887028377296 2316 66-67 -1.502323689446996 2316 68-69 -1.4568043254966128 2316 70-71 -1.3904864965958552 2316 72-73 -1.5885482182101782 2316 74-75 -1.2580182009133054 2316 76-77 -1.4191069670866696 2316 78-79 -1.1447934105955042 2316 80-81 -1.1016280874048192 2316 82-83 -1.163666577392764 2316 84-85 -1.1931231867342902 2316 86-87 -1.1667903175151118 2316 88-89 -1.2799929702879709 2316 90-91 -1.263788919961069 2316 92-93 -1.3811577985831995 2316 94-95 -1.2739318528188157 2316 96-97 -1.4703142877754267 2316 98-99 -1.6757440891622757 2316 100-101 -1.281621793622513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8840.0 3 2583.0 4 2628.0 5 2762.0 6 3105.0 7 3879.0 8 4547.0 9 5677.0 10 6823.0 11 7976.0 12 10256.0 13 15402.0 14 13356.0 15 12899.0 16 14576.0 17 19366.0 18 17281.0 19 13652.0 20 14046.0 21 14510.0 22 15804.0 23 17255.0 24 19483.0 25 21833.0 26 24850.0 27 28942.0 28 34050.0 29 40483.0 30 48946.0 31 59652.0 32 75224.0 33 98235.0 34 136723.0 35 203673.0 36 333514.0 37 541368.0 38 539344.0 39 98223.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.230364054937745 18.436531267867892 14.513347042127977 23.819757635066388 2 15.352052748003642 19.575507324661682 40.83956606431213 24.23287386302254 3 19.58699860210653 23.23192179705434 29.40100165888631 27.78007794195282 4 13.165499520868423 16.22850087382684 36.697919307361225 33.90808029794351 5 14.442585171172889 36.429979113826676 34.21061061900353 14.916825095996902 6 32.88537999352557 35.26364739870456 17.346264809081283 14.50470779868859 7 29.08517000994187 31.89743468111895 20.574335742705177 18.44305956623401 8 26.014042349994625 35.48934767346991 19.612171196184956 18.884438780350514 9 25.949751619994206 17.447909769692618 20.39352626875005 36.208812341563124 10-11 25.230509377697807 26.207155257740432 27.122334605618082 21.44000075894368 12-13 25.920646487837338 23.391483796396496 27.6728291808159 23.015040534950266 14-15 22.769868800930947 25.08275043717212 25.8248362778549 26.322544484042034 16-17 22.668851247256512 28.106599100145168 26.534293995400915 22.690255657197405 18-19 22.50506206114638 27.049037753765216 28.30105289290568 22.144847292182725 20-21 23.119632027346135 26.17541713865328 28.5222093460168 22.182741487983787 22-23 22.722887908063136 26.036476017123512 28.500866612793292 22.739769462020064 24-25 22.667439023666944 26.244585428153993 28.537418126533424 22.550557421645635 26-27 22.55847209505871 26.432959517090808 28.457371879974165 22.551196507876316 28-29 22.372288545922824 26.78221936138935 28.426093378955535 22.41939871373229 30-31 22.62799347917747 26.354556184571614 28.31382733420164 22.703623002049273 32-33 22.44107562411271 26.39225751155083 28.415645010359874 22.75102185397658 34-35 22.97165097731575 26.530541476300318 28.09312909667027 22.40467844971366 36-37 22.955491885813753 26.317244988390154 28.17347031151233 22.553792814283764 38-39 22.61726545420477 26.533749211494843 28.231799276460922 22.617186057839458 40-41 22.91611972753154 26.394821297497383 28.189496030189254 22.499562944781818 42-43 22.723362428339307 26.512362697891895 27.8956756063643 22.8685992674045 44-45 22.75428917858709 26.57614298194959 28.00750689379242 22.662060945670902 46-47 22.948089728612732 26.787311903303834 27.604461220394093 22.66013714768934 48-49 22.968929100763543 26.739526867273415 27.60004430929316 22.69149972266988 50-51 22.824712662502986 26.94493891553309 27.593313096578793 22.637035325385135 52-53 22.992730434506417 26.92121394994283 27.361596366408335 22.72445924914242 54-55 22.801509644898378 26.81185872794969 27.456552450790937 22.930079176360994 56-57 22.82742280942363 26.99682226198667 27.389093212523857 22.786661716065844 58-59 22.96645087680135 26.74750601483245 27.426743061239677 22.85930004712652 60-61 22.82106843922767 26.79372241777194 27.6930329562754 22.692176186724993 62-63 22.645952269239533 26.855003187502135 27.77864445021344 22.720400093044894 64-65 22.56123453961755 26.90196378943724 27.624237440663357 22.912564230281852 66-67 22.576535905109434 26.891510442612045 27.442415285003463 23.089538367275058 68-69 22.63741169167739 26.71915903133239 27.66458951684374 22.97883976014648 70-71 22.785671495031018 26.605618625205835 27.485021771621653 23.123688108141486 72-73 22.709766083113667 26.666949915908727 27.51504976682565 23.108234234151954 74-75 22.59149286373036 27.04303204843749 27.242095105919773 23.123379981912386 76-77 22.728856223570837 26.83369728484431 27.26482152083893 23.172624970745918 78-79 22.71869614605171 26.832076096249086 27.374336487605305 23.074891270093897 80-81 22.76724492084315 26.836603667987767 27.26569988866242 23.13045152250666 82-83 22.854373664919574 26.82399625572092 27.33929115144124 22.98233892791826 84-85 22.813339253280798 26.83828163005502 27.22197996837446 23.12639914828972 86-87 22.825062843680254 26.73299201416352 27.321385508391234 23.120559633764994 88-89 22.833911531252166 26.7826556107364 27.102637888641006 23.28079496937043 90-91 22.850164069299822 26.69628279003577 27.178405112990223 23.275148027674184 92-93 22.831091841501838 26.54363528101591 27.332719030155456 23.2925538473268 94-95 22.776577060939015 26.47354295559527 27.397759611944554 23.35212037152116 96-97 22.800030654675872 26.347356391579336 27.420845814921996 23.4317671388228 98-99 22.694463917953556 26.405780544902207 27.216063828372107 23.683691708772134 100-101 23.115574697562597 25.687955025723006 27.641526174983156 23.554944101731238 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1980.0 1 1087.5 2 219.5 3 259.0 4 254.0 5 264.0 6 413.5 7 833.0 8 1407.5 9 1768.5 10 1778.5 11 1783.5 12 2005.0 13 2234.5 14 2457.5 15 2685.5 16 2890.5 17 3117.0 18 3263.5 19 3482.5 20 3843.5 21 4308.0 22 5140.0 23 6334.5 24 7872.0 25 10091.5 26 12860.5 27 16161.0 28 19271.0 29 22501.0 30 26822.5 31 32183.5 32 38297.5 33 42697.5 34 46212.5 35 50356.5 36 55156.0 37 60294.0 38 64477.0 39 67594.0 40 70924.0 41 75488.0 42 79558.0 43 83223.5 44 86741.0 45 90231.5 46 92845.0 47 96086.5 48 100992.5 49 100727.5 50 95561.0 51 91878.0 52 90267.5 53 89577.5 54 89043.5 55 84321.5 56 77158.5 57 70351.0 58 64018.0 59 56560.0 60 47173.5 61 39269.0 62 32645.5 63 25940.0 64 19257.5 65 14106.5 66 10314.5 67 8166.5 68 6316.5 69 4588.0 70 3498.5 71 2402.0 72 1785.0 73 1416.5 74 1078.5 75 774.0 76 560.0 77 378.0 78 250.5 79 175.5 80 133.0 81 91.0 82 57.5 83 39.5 84 28.0 85 24.5 86 23.5 87 18.0 88 11.5 89 11.5 90 13.0 91 11.5 92 8.5 93 4.5 94 4.0 95 2.0 96 2.5 97 4.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38814144352911295 2 0.11671496546540439 3 0.11865034052726726 4 0.12939364699311837 5 0.13200047870909692 6 0.0733862625498211 7 0.0831026353093776 8 0.08029831634218854 9 0.07962685968807284 10-11 0.07808645912863095 12-13 0.08209545032820406 14-15 0.07555862231313658 16-17 0.0772767613986679 18-19 0.08717087268431387 20-21 0.08549223104902463 22-23 0.09562332703612318 24-25 0.10877597796674235 26-27 0.11073110175372627 28-29 0.1039177915869641 30-31 0.09374719814962344 32-33 0.09234503866602892 34-35 0.3355110910815152 36-37 0.5236176978595147 38-39 0.5056463579993594 40-41 0.47809688645549486 42-43 0.5063573121037171 44-45 0.5157379565362158 46-47 0.5131311248202373 48-49 0.51939147068361 50-51 0.5183842857024364 52-53 0.5169821262188419 54-55 0.511037759957406 56-57 0.4838240167405993 58-59 0.4738311618293481 60-61 0.42305718954312904 62-63 0.49747038579924474 64-65 0.5227290052290674 66-67 0.5331168346427396 68-69 0.5472569218294113 70-71 0.5425962227008436 72-73 0.5298385462726454 74-75 0.533669798946129 76-77 0.5106822829052271 78-79 0.4843572323188676 80-81 0.4915062708126877 82-83 0.5042244497906437 84-85 0.4993465146857444 86-87 0.5000969662403444 88-89 0.510287308402806 90-91 0.527725432684693 92-93 0.5335513065954027 94-95 0.5295028179455875 96-97 0.5305692491021242 98-99 0.530470505476519 100-101 0.507858215212917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2531809.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.297695328099934 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41028995967855 45.30954093960792 2 13.631525934459601 16.166361434373563 3 4.461977117381154 7.937548790022637 4 1.9460367845690507 4.615819853946032 5 1.02665899601988 3.0439256175919907 6 0.6186866707210437 2.201201622238376 7 0.42208313189772906 1.7519989870881227 8 0.2623462139598085 1.2445220692695393 9 0.19454533740140134 1.0382481130257633 >10 0.9411578772598754 9.915668848388792 >50 0.05652204440420584 2.3064340938033876 >100 0.025766559556451237 2.8705882931388187 >500 0.0016690088133845427 0.665186249548559 >1k 6.676035253439949E-4 0.7137599285380202 >5k 6.676035253439949E-5 0.2191951594184702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.21869738199050562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4719 0.1863884676924681 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.11462160060257309 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.9497450242099624E-5 3 0.0 0.0 0.0 0.0 3.9497450242099624E-5 4 0.0 0.0 0.0 0.0 3.9497450242099624E-5 5 0.0 0.0 0.0 0.0 3.9497450242099624E-5 6 0.0 0.0 0.0 0.0 1.1849235072629886E-4 7 0.0 0.0 0.0 0.0 1.1849235072629886E-4 8 0.0 0.0 0.0 0.0 1.1849235072629886E-4 9 0.0 0.0 0.0 0.0 1.579898009683985E-4 10-11 0.0 0.0 0.0 0.0 1.579898009683985E-4 12-13 0.0 0.0 0.0 0.0 1.579898009683985E-4 14-15 0.0 0.0 0.0 0.0 1.777385260894483E-4 16-17 0.0 0.0 0.0 0.0 2.3698470145259773E-4 18-19 0.0 0.0 0.0 0.0 2.3698470145259773E-4 20-21 0.0 0.0 0.0 5.924617536314944E-5 2.3698470145259773E-4 22-23 0.0 0.0 0.0 2.3698470145259773E-4 2.7648215169469737E-4 24-25 0.0 0.0 0.0 3.752257772999464E-4 2.7648215169469737E-4 26-27 0.0 0.0 0.0 4.937181280262453E-4 3.15979601936797E-4 28-29 0.0 0.0 0.0 0.0024290931898891268 3.15979601936797E-4 30-31 0.0 0.0 0.0 0.006477581839704339 3.15979601936797E-4 32-33 0.0 0.0 0.0 0.015147272167845206 3.554770521788966E-4 34-35 0.0 0.0 0.0 0.027746958795074984 3.949745024209962E-4 36-37 0.0 0.0 0.0 0.050043269456740216 3.949745024209962E-4 38-39 0.0 0.0 0.0 0.08312238403449865 3.949745024209962E-4 40-41 0.0 0.0 0.0 0.11693220144173594 3.949745024209962E-4 42-43 0.0 0.0 0.0 0.15376357379249384 3.949745024209962E-4 44-45 0.0 0.0 0.0 0.2030761404197552 4.1472322754204606E-4 46-47 0.0 0.0 0.0 0.26437618319549383 4.7396940290519546E-4 48-49 0.0 0.0 0.0 0.33090963812831065 4.7396940290519546E-4 50-51 0.0 0.0 0.0 0.4023802743413899 4.7396940290519546E-4 52-53 0.0 3.9497450242099624E-5 0.0 0.4753913111139111 5.529643033893947E-4 54-55 0.0 3.9497450242099624E-5 0.0 0.560192336783699 6.31959203873594E-4 56-57 0.0 3.9497450242099624E-5 0.0 0.6610688247020213 6.31959203873594E-4 58-59 0.0 3.9497450242099624E-5 0.0 0.7741302760200315 6.912053792367434E-4 60-61 0.0 3.9497450242099624E-5 0.0 0.9008380963966871 7.504515545998928E-4 62-63 0.0 3.9497450242099624E-5 0.0 1.0318313901246103 8.29446455084092E-4 64-65 0.0 3.9497450242099624E-5 0.0 1.1713956305550695 8.29446455084092E-4 66-67 0.0 3.9497450242099624E-5 0.0 1.336593716192651 8.29446455084092E-4 68-69 0.0 3.9497450242099624E-5 0.0 1.5309804175591446 8.29446455084092E-4 70-71 0.0 3.9497450242099624E-5 0.0 1.740988360496388 8.29446455084092E-4 72-73 0.0 3.9497450242099624E-5 0.0 1.9538203711259419 9.874362560524906E-4 74-75 0.0 3.9497450242099624E-5 0.0 2.1751245848324263 9.874362560524906E-4 76-77 0.0 3.9497450242099624E-5 0.0 2.4263086196470587 9.874362560524906E-4 78-79 0.0 3.9497450242099624E-5 0.0 2.715943422272375 9.874362560524906E-4 80-81 0.0 3.9497450242099624E-5 0.0 3.0305801109009405 0.0010071849811735404 82-83 0.0 3.9497450242099624E-5 0.0 3.354478951611279 0.0010269337062945901 84-85 0.0 3.9497450242099624E-5 0.0 3.6878769291048417 0.0010269337062945901 86-87 0.0 3.9497450242099624E-5 0.0 4.046494028577985 0.00106643115653669 88-89 0.0 3.9497450242099624E-5 0.0 4.437597780875256 0.0011256773318998393 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6575 0.0 31.561777 1 GTATCAA 9535 0.0 29.632755 1 ATCAACG 11280 0.0 24.43738 3 TCAACGC 11400 0.0 24.304785 4 TATCAAC 11565 0.0 24.039932 2 CAACGCA 11575 0.0 23.814571 5 AACGCAG 11765 0.0 23.419325 6 GAGTACT 6840 0.0 20.868109 12-13 ACGCAGA 13175 0.0 20.769236 7 CGCAGAG 13235 0.0 20.638903 8 GTACATG 7625 0.0 20.302687 1 AGAGTAC 10535 0.0 20.019451 10-11 TACATGG 7420 0.0 19.91551 2 GTACTTT 7185 0.0 19.669003 14-15 AGTACTT 7360 0.0 18.879137 12-13 ACTGCGT 255 2.7741953E-7 18.564983 8 GCAGAGT 14695 0.0 18.201769 9 ACATGGG 7715 0.0 18.171741 3 TAGCCCG 160 0.0012148281 17.761189 5 GTATAGC 990 0.0 17.268042 1 >>END_MODULE