FastQCFastQC Report
Fri 27 May 2016
SRR939222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531809
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57010.22517496383020996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50860.20088403193131865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.12267908045196144No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26460.10451025334059559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.031.4002151
GTATCAA93550.031.1761841
ATCAACG110850.025.8115793
TCAACGC111750.025.5612374
TATCAAC114650.025.1216162
CAACGCA114400.024.969135
AACGCAG118000.024.2475686
ACGCAGA131350.021.7108757
CGCAGAG133200.021.3737138
GAGTACT74400.020.98533612-13
TACATGG69600.020.725132
AGAGTAC106900.020.53139310-11
GTACATG72800.020.227511
GTATAGC8450.019.7390691
GTACTTT79100.019.52843714-15
ACATGGG70650.019.342533
GTAAGCG2001.8189894E-1218.9802394-95
GCAGAGT148750.018.9479589
AGTACTT80700.018.49439212-13
CATGGGG49200.017.2631824
Produced by FastQC (version 0.11.5)