FastQCFastQC Report
Fri 27 May 2016
SRR939221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580392
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184011.164331381075075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141570.8957904114928448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103940.6576849288024743No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57390.36313775316503755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32050.20279778687819225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31840.20146900262719628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.15673326617699912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23650.14964641683835403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.13964889723562254No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16650.1053536084718222No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.10345534525611366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105750.034.1161841
GTATCAA160150.032.487091
GTGTAAG5000.030.3813571
TATCAAC193700.026.7837562
ATCAACG197200.026.236313
TCAACGC198150.026.1105214
CAACGCA200850.025.7595235
TAAGGTG8350.025.5336595
AACGCAG205050.025.3600866
GTAAGGT7900.025.18894
CGACCGT851.1525299E-725.08227218-19
TAAGGTA4350.025.0510314
GTACATG121550.024.9168381
TACATGG120500.024.5742342
GGTAAGG7550.023.8464453
GAGTACT116700.023.80042512-13
ACGCAGA227800.022.7026797
CGCAGAG229150.022.5275948
ACATGGG125350.022.5272883
AGGTAAG7000.022.335872
Produced by FastQC (version 0.11.5)