##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939221_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1580392 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13513482730867 33.0 31.0 34.0 30.0 34.0 2 32.206336149512275 34.0 31.0 34.0 30.0 34.0 3 32.23816749262208 34.0 31.0 34.0 30.0 34.0 4 35.71097866858349 37.0 35.0 37.0 35.0 37.0 5 35.765984641785074 37.0 35.0 37.0 35.0 37.0 6 35.87164323788022 37.0 37.0 37.0 35.0 37.0 7 35.804161878825 37.0 36.0 37.0 35.0 37.0 8 35.79447124510881 37.0 36.0 37.0 35.0 37.0 9 37.46155004581142 39.0 38.0 39.0 35.0 39.0 10-11 37.386559790229256 39.0 38.0 39.0 35.0 39.0 12-13 37.285667733068756 39.0 37.0 39.0 34.0 39.0 14-15 38.53762484244415 40.0 38.0 41.0 34.0 41.0 16-17 38.494207449797265 40.0 38.0 41.0 34.0 41.0 18-19 38.4851233111785 40.0 38.0 41.0 34.0 41.0 20-21 38.41851768422012 40.0 38.0 41.0 34.0 41.0 22-23 38.32474063396929 40.0 38.0 41.0 34.0 41.0 24-25 38.314825372439245 40.0 38.0 41.0 34.0 41.0 26-27 38.20930123665521 40.0 38.0 41.0 34.0 41.0 28-29 38.061099081746804 40.0 38.0 41.0 33.5 41.0 30-31 37.79792228763497 40.0 37.5 41.0 33.0 41.0 32-33 37.496289844544904 40.0 36.5 41.0 33.0 41.0 34-35 37.14630737184193 40.0 36.0 41.0 32.0 41.0 36-37 36.85547066803679 40.0 35.0 41.0 31.0 41.0 38-39 36.58291328986732 39.5 35.0 41.0 30.5 41.0 40-41 36.370132853114924 39.0 35.0 41.0 30.0 41.0 42-43 36.092884233785036 39.0 35.0 41.0 30.0 41.0 44-45 35.71427785005239 39.0 35.0 41.0 28.5 41.0 46-47 35.17502967618161 39.0 35.0 41.0 25.5 41.0 48-49 34.95542340128272 39.0 35.0 41.0 24.0 41.0 50-51 33.878078982935875 38.0 34.0 40.0 22.0 40.5 52-53 34.000504305260975 38.0 33.5 39.5 22.0 40.5 54-55 34.436691023492905 38.0 35.0 40.5 22.5 41.0 56-57 34.28377674652871 38.0 35.0 41.0 21.5 41.0 58-59 34.06699666918081 38.0 34.5 40.0 20.5 41.0 60-61 33.748353573037576 37.0 34.0 40.0 19.5 41.0 62-63 33.36767365311897 37.0 34.0 40.0 17.5 41.0 64-65 32.98298618317481 36.0 33.5 39.5 14.0 41.0 66-67 32.579113599663884 35.5 33.0 39.0 9.5 41.0 68-69 32.173782200871685 35.0 33.0 39.0 7.0 41.0 70-71 31.75940304683901 35.0 32.5 38.0 3.5 40.0 72-73 31.322605404228824 35.0 32.0 37.0 2.0 39.5 74-75 30.891179213764687 35.0 31.5 37.0 2.0 39.0 76-77 30.481904173141853 35.0 31.0 36.0 2.0 39.0 78-79 30.085065287599534 35.0 31.0 36.0 2.0 37.5 80-81 29.692273499233103 35.0 30.5 35.0 2.0 37.0 82-83 29.290508620646015 34.0 30.0 35.0 2.0 37.0 84-85 28.93727632131775 34.0 29.5 35.0 2.0 36.0 86-87 28.57723685009795 34.0 29.0 35.0 2.0 36.0 88-89 28.194251173126666 34.0 29.0 35.0 2.0 35.5 90-91 27.837030939159398 34.0 28.5 35.0 2.0 35.0 92-93 27.44600959761882 34.0 27.0 35.0 2.0 35.0 94-95 27.004173015302534 34.0 26.5 35.0 2.0 35.0 96-97 26.459850151101755 34.0 25.0 35.0 2.0 35.0 98-99 25.806383163164583 33.0 22.0 35.0 2.0 35.0 100-101 23.474729054563678 31.0 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1743864682808436 1101 2 0.343355735518152 1101 3 0.2620637773936352 1101 4 0.19762359322444922 1101 5 0.2045728367098576 1101 6 0.27698084354532426 1101 7 0.1186034572537693 1101 8 0.25904888186973096 1101 9 0.336374758083096 1101 10-11 0.292021191198927 1101 12-13 0.22599234510563093 1101 14-15 0.39917344626209683 1101 16-17 0.38634798307389673 1101 18-19 0.4184991561463107 1101 20-21 0.31837518542604215 1101 22-23 0.3690990488282182 1101 24-25 0.3607182842663761 1101 26-27 0.3871437132058233 1101 28-29 0.3928472591855865 1101 30-31 0.39435829349393003 1101 32-33 0.45251343181273285 1101 34-35 0.3739154074654394 1101 36-37 0.41541607547713966 1101 38-39 0.3650592027677675 1101 40-41 0.26976566873921826 1101 42-43 0.24602772318144162 1101 44-45 0.2123275470877104 1101 46-47 0.14208742481442727 1101 48-49 0.2903319469104986 1101 50-51 0.18045239104670685 1101 52-53 0.25400726155576336 1101 54-55 0.17446496428979685 1101 56-57 0.3291696713579313 1101 58-59 0.34732429340398596 1101 60-61 0.4037383854067613 1101 62-63 0.30616800773744046 1101 64-65 0.37689494104260746 1101 66-67 0.362464487936478 1101 68-69 0.1588294927793541 1101 70-71 0.1550572450277592 1101 72-73 0.06495204234169805 1101 74-75 -0.08546957851757142 1101 76-77 -0.21459981729097422 1101 78-79 -0.07729218199465038 1101 80-81 -0.23455808828594016 1101 82-83 -0.13884355423356354 1101 84-85 -0.05328756874292395 1101 86-87 -0.1959343042282562 1101 88-89 -0.33613993778805096 1101 90-91 -0.39519821151893453 1101 92-93 -0.4886215201871771 1101 94-95 -0.4850211230303678 1101 96-97 -0.5505027726216305 1101 98-99 -0.5260419878011113 1101 100-101 -0.47608781009124357 1102 1 0.12082746062955607 1102 2 0.16675785314702551 1102 3 0.22329854566526564 1102 4 0.22380735291736187 1102 5 0.17445394550239257 1102 6 0.18962865249501704 1102 7 0.14776271737175506 1102 8 0.22561636518889117 1102 9 0.25997459835926406 1102 10-11 0.24887482491610768 1102 12-13 0.2812725702647114 1102 14-15 0.3488153557347573 1102 16-17 0.26265378980805565 1102 18-19 0.2534570998307828 1102 20-21 0.26454567773610904 1102 22-23 0.24307179272908996 1102 24-25 0.2522896318718111 1102 26-27 0.36348140030167997 1102 28-29 0.27930486796525145 1102 30-31 0.25248263334502496 1102 32-33 0.14509496825295543 1102 34-35 0.2306035209208801 1102 36-37 0.15430303222289865 1102 38-39 0.18844199896530966 1102 40-41 -0.03933375934641248 1102 42-43 0.015634145625220697 1102 44-45 0.11298962813467739 1102 46-47 0.01726614805424731 1102 48-49 8.119721378818667E-6 1102 50-51 0.03837224574954945 1102 52-53 0.04233937119844455 1102 54-55 0.11632516729363118 1102 56-57 0.04522674686609207 1102 58-59 0.1800393409346981 1102 60-61 0.001337375539947061 1102 62-63 0.13957701667681732 1102 64-65 0.23648770967490407 1102 66-67 0.09249095829740384 1102 68-69 0.07997229103784065 1102 70-71 0.07403121261927836 1102 72-73 -0.06355157720643945 1102 74-75 -0.11301549911937414 1102 76-77 -0.06648816517978418 1102 78-79 -0.11646477390025822 1102 80-81 -0.0889823740370268 1102 82-83 -0.31134368304310556 1102 84-85 -0.29124387654620776 1102 86-87 -0.520956201850435 1102 88-89 -0.4817092115598527 1102 90-91 -0.5124873502983327 1102 92-93 -0.7096442035475619 1102 94-95 -0.6687663248749232 1102 96-97 -0.8647207698908659 1102 98-99 -1.1554118515206149 1102 100-101 -1.0964168540668204 1103 1 0.10115252072323244 1103 2 0.17634222762908536 1103 3 0.24245673798827028 1103 4 0.2265916670154411 1103 5 0.1859416823802249 1103 6 0.18213238162849166 1103 7 0.1431130447859843 1103 8 0.1714988000992861 1103 9 0.20772767007791515 1103 10-11 0.2666920598995546 1103 12-13 0.24388015563362586 1103 14-15 0.3264460970959888 1103 16-17 0.29994063802784154 1103 18-19 0.37579695822400083 1103 20-21 0.3716058537575435 1103 22-23 0.32296075797768253 1103 24-25 0.27871923860525527 1103 26-27 0.2415944218129482 1103 28-29 0.4482425320550334 1103 30-31 0.4038115561136948 1103 32-33 0.4031114176111643 1103 34-35 0.3040481845915721 1103 36-37 0.1870584422375572 1103 38-39 0.2641977752954219 1103 40-41 0.21019805361945743 1103 42-43 0.17848748959964666 1103 44-45 0.12328604806096877 1103 46-47 0.06542211398163289 1103 48-49 0.14759101838501465 1103 50-51 0.2808361235627572 1103 52-53 0.3686380227986703 1103 54-55 0.24734865476447965 1103 56-57 0.16673210277797068 1103 58-59 0.18327785451740652 1103 60-61 0.1398531751368992 1103 62-63 0.09033160538479734 1103 64-65 -0.04236015962543149 1103 66-67 0.012559238652229965 1103 68-69 -0.06297967307801855 1103 70-71 0.06474110450871606 1103 72-73 0.023974853983791178 1103 74-75 7.278224855333804E-4 1103 76-77 0.012126085652340635 1103 78-79 -0.13952311728879252 1103 80-81 -0.05564661267623805 1103 82-83 0.00957952689364916 1103 84-85 -0.044454678945943016 1103 86-87 -0.15739656580119288 1103 88-89 0.018233782578043645 1103 90-91 -0.15763535968529752 1103 92-93 -0.386805491077741 1103 94-95 -0.38970573925580965 1103 96-97 -0.312341619679362 1103 98-99 -0.40159430617192626 1103 100-101 -0.7697101882869113 1104 1 0.23549574041295074 1104 2 0.1809003307588739 1104 3 0.17637655094176097 1104 4 0.1637633924079438 1104 5 0.21102305554835254 1104 6 0.20376189470572115 1104 7 0.19407055791947414 1104 8 0.24026371139637348 1104 9 0.3706855087543275 1104 10-11 0.27702201873947274 1104 12-13 0.24796124797075692 1104 14-15 0.3631294578844404 1104 16-17 0.37548191591438496 1104 18-19 0.38252183075736923 1104 20-21 0.30252318645587195 1104 22-23 0.3598857640439306 1104 24-25 0.4083340479943587 1104 26-27 0.3454908388816449 1104 28-29 0.4498142311227724 1104 30-31 0.4710276266801472 1104 32-33 0.49279396805901143 1104 34-35 0.40693657411371476 1104 36-37 0.41641372158402135 1104 38-39 0.447206394646841 1104 40-41 0.3996964311799118 1104 42-43 0.3497024629659151 1104 44-45 0.3665075463521248 1104 46-47 0.38811752890064355 1104 48-49 0.5020168194692545 1104 50-51 0.5451541086530867 1104 52-53 0.5258888129184456 1104 54-55 0.4473211745218748 1104 56-57 0.5048634261852527 1104 58-59 0.4997296779344893 1104 60-61 0.6044629431323116 1104 62-63 0.6953488345423935 1104 64-65 0.41512961315264363 1104 66-67 0.17283586974529896 1104 68-69 0.17384475630503715 1104 70-71 0.3533474543035702 1104 72-73 0.3783554407748859 1104 74-75 0.41046627789494394 1104 76-77 0.25944299414011596 1104 78-79 0.21792601286356472 1104 80-81 0.1172041133397137 1104 82-83 0.029517271835803882 1104 84-85 0.04158535780825545 1104 86-87 -0.08018436859252276 1104 88-89 0.20686228737653423 1104 90-91 0.02660610272580044 1104 92-93 0.03992173259887366 1104 94-95 -0.277830255400076 1104 96-97 -0.22703556161717842 1104 98-99 -0.20310808070633257 1104 100-101 -0.4363773573346137 1105 1 0.021675144516883904 1105 2 -0.013139842269296764 1105 3 0.15405788111022645 1105 4 0.037656699405879124 1105 5 0.030541857056867627 1105 6 0.11763620094709637 1105 7 0.049726892563803915 1105 8 0.027293529300976616 1105 9 0.04560219996604076 1105 10-11 0.12722429883025654 1105 12-13 0.1949432174655925 1105 14-15 0.1996060014793386 1105 16-17 0.21802902797264778 1105 18-19 0.18159965878030704 1105 20-21 0.10275607566817513 1105 22-23 0.05407681693996125 1105 24-25 0.023264802076056412 1105 26-27 0.08114714464944228 1105 28-29 -0.0974927437142199 1105 30-31 -0.007993574467981546 1105 32-33 -0.007296993009653363 1105 34-35 0.20667784040729487 1105 36-37 0.13194741760656825 1105 38-39 0.31012589843254545 1105 40-41 0.22321463205660308 1105 42-43 0.19068663285231935 1105 44-45 0.270643964853889 1105 46-47 0.1631563678331318 1105 48-49 0.36498396952902823 1105 50-51 0.3035131420978914 1105 52-53 0.44586906137210036 1105 54-55 0.4923779457793316 1105 56-57 0.3541171122742739 1105 58-59 0.2314768234895226 1105 60-61 0.2821698290295913 1105 62-63 0.3471852374508373 1105 64-65 0.40773866536719083 1105 66-67 0.3117449722050054 1105 68-69 0.29081924677867477 1105 70-71 0.1850189433507019 1105 72-73 0.11991555304683388 1105 74-75 0.09720646420631596 1105 76-77 0.21398628294583943 1105 78-79 0.23740684173547422 1105 80-81 0.030705190434275664 1105 82-83 -0.025047096836626537 1105 84-85 0.14357942656904754 1105 86-87 0.245193480799049 1105 88-89 0.2704130321022369 1105 90-91 0.035910120606146734 1105 92-93 0.05198490289532032 1105 94-95 -0.09441953296705563 1105 96-97 -0.09560692418510897 1105 98-99 -0.20729228986481374 1105 100-101 -0.12558215545879392 1106 1 0.15604622514965172 1106 2 0.13957525628267575 1106 3 0.2364487273073621 1106 4 0.2398914513432615 1106 5 0.3237151238919864 1106 6 0.19671592892232326 1106 7 0.24822755644255068 1106 8 0.2630054243161908 1106 9 0.2635456838398582 1106 10-11 0.1993972052215156 1106 12-13 0.27017162117742544 1106 14-15 0.3380256390493628 1106 16-17 0.42053733105444024 1106 18-19 0.3322003678984373 1106 20-21 0.49528357790255484 1106 22-23 0.36825191541836944 1106 24-25 0.4544407133921524 1106 26-27 0.4914660455163471 1106 28-29 0.4096269420375762 1106 30-31 0.39875353002762637 1106 32-33 0.48884110848104 1106 34-35 0.33840168156189776 1106 36-37 0.285651438026818 1106 38-39 0.5130167133666959 1106 40-41 0.4017354881475441 1106 42-43 0.33164041144415535 1106 44-45 0.15322340042823157 1106 46-47 0.2794767509334619 1106 48-49 0.575905694363243 1106 50-51 0.5539181816915502 1106 52-53 0.617148857758103 1106 54-55 0.4126973247860519 1106 56-57 0.5670350708379459 1106 58-59 0.5395220775840528 1106 60-61 0.6286074115546825 1106 62-63 0.6133109624763122 1106 64-65 0.5263725095745215 1106 66-67 0.5893796145380321 1106 68-69 0.6008028061250243 1106 70-71 0.4138989560030204 1106 72-73 0.6082153798130996 1106 74-75 0.6705696628881235 1106 76-77 0.5858397551604 1106 78-79 0.5741207155139207 1106 80-81 0.6936088048034819 1106 82-83 0.7948316579401755 1106 84-85 0.7216950591664109 1106 86-87 0.6646995623697016 1106 88-89 0.7730607313302258 1106 90-91 0.7906289927846615 1106 92-93 0.7334830988370165 1106 94-95 0.7017752097017755 1106 96-97 0.6631641829292398 1106 98-99 0.7079620253470225 1106 100-101 0.5413199031170137 1107 1 0.20059671314433558 1107 2 0.2857987414286498 1107 3 0.1923417897149875 1107 4 0.25457859212990996 1107 5 0.16678395720271055 1107 6 0.13533997667226316 1107 7 0.13069026965017372 1107 8 0.23303408998806674 1107 9 0.20706306530391316 1107 10-11 0.19520355664909061 1107 12-13 0.32042936233676045 1107 14-15 0.36299119026560334 1107 16-17 0.3436063250071584 1107 18-19 0.37413328380056043 1107 20-21 0.3210958909331225 1107 22-23 0.27463466817962257 1107 24-25 0.34723984663933294 1107 26-27 0.32667056062739874 1107 28-29 0.2638951998929713 1107 30-31 0.3649504378476891 1107 32-33 0.32841642458879505 1107 34-35 0.3444391432755509 1107 36-37 0.28656174267544543 1107 38-39 0.2912776566565043 1107 40-41 0.32158807234180387 1107 42-43 0.38304808156402714 1107 44-45 0.6019415665391961 1107 46-47 0.6233532565059576 1107 48-49 0.48606442650520165 1107 50-51 0.3275874412390323 1107 52-53 0.45858985216775494 1107 54-55 0.47436020149153535 1107 56-57 0.3990753848395059 1107 58-59 0.2315477196165716 1107 60-61 0.1220965021781879 1107 62-63 0.03709570577039756 1107 64-65 0.1500717556280904 1107 66-67 0.0391696197499698 1107 68-69 0.1309383522721106 1107 70-71 0.2667723058527294 1107 72-73 0.29164622878820623 1107 74-75 0.3030381770567452 1107 76-77 0.19023608038547835 1107 78-79 0.24079972781552783 1107 80-81 0.15368971482254423 1107 82-83 0.16730422385694155 1107 84-85 0.25067309048089115 1107 86-87 0.07178155404487896 1107 88-89 0.24562977169063416 1107 90-91 0.06054146074550815 1107 92-93 0.18096521402803845 1107 94-95 0.07106215557612927 1107 96-97 0.11856014760228462 1107 98-99 0.18929051681157816 1107 100-101 0.23989750748076233 1108 1 -0.12589125304950954 1108 2 0.004434107122840203 1108 3 0.09330291126434531 1108 4 -0.036952147930243484 1108 5 0.022899622141899556 1108 6 0.08276218880035913 1108 7 0.09726350828223929 1108 8 0.11077843096769868 1108 9 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-0.6851452414053796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5676.0 3 1292.0 4 1266.0 5 1418.0 6 1592.0 7 1964.0 8 2556.0 9 3079.0 10 3623.0 11 4882.0 12 6537.0 13 10593.0 14 9881.0 15 11263.0 16 14832.0 17 21749.0 18 20254.0 19 11623.0 20 10834.0 21 11111.0 22 11272.0 23 11898.0 24 13164.0 25 14413.0 26 16281.0 27 19152.0 28 22207.0 29 26187.0 30 31077.0 31 38212.0 32 48006.0 33 62659.0 34 88221.0 35 146126.0 36 219856.0 37 321914.0 38 284850.0 39 48859.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.5679646478435 18.472581126469038 14.897238747690492 21.062215477996965 2 15.90451828370595 20.83273152054652 39.5493625852357 23.713387610511834 3 19.412488700853064 26.025759837938402 29.929199402784263 24.63255205842427 4 12.818795900306418 16.998309215315 36.08574159818112 34.097153286197454 5 13.495445274547585 37.939149096056326 32.55363056953552 16.011775059860568 6 30.808203310785608 35.7903409770991 17.26322829145401 16.138227420661284 7 28.108218004201696 34.71439058019985 19.59475250603748 17.582638909560973 8 27.743543067645383 32.81199031927272 19.217865134877158 20.226601478204746 9 28.192124029436727 16.592800415458083 19.862126182725557 35.35294937237964 10-11 25.577903306859916 26.921755497368437 25.367802708730274 22.13253848704137 12-13 27.387421051048243 24.680522943289755 26.69255944228671 21.239496563375287 14-15 23.905185883270917 25.251960041066017 26.67305710672035 24.169796968942713 16-17 21.636323106511817 28.811848395556154 27.121373607340466 22.430454890591562 18-19 21.65558318815924 25.939171606702722 31.26867852993141 21.13656667520663 20-21 22.830877986441354 24.754900796384362 31.479083231588067 20.93513798558622 22-23 22.31306350179196 24.996682139457835 31.561579966773884 21.128674391976322 24-25 21.937630237949364 25.387728155979527 31.62175826696174 21.052883339109368 26-27 21.442851553899835 25.555075101330964 31.686315132565767 21.31575821220343 28-29 21.281144358791124 25.97902633966357 31.638987719645016 21.100841581900287 30-31 21.31839012998547 25.61602863804277 31.488765833259162 21.576815398712593 32-33 21.13545416107797 25.819729113847877 31.372657696248357 21.672159028825796 34-35 21.933638926970822 25.90993251271041 30.93759433412763 21.218834226191138 36-37 22.052080965378444 25.479040916670776 31.01082728329139 21.458050834659385 38-39 21.59412048716654 25.80544678811396 30.967629559160496 21.632803165559004 40-41 21.648400119997355 25.811253311367715 31.096189829715616 21.44415673891931 42-43 21.538924679773483 26.019711724610602 30.45850549727219 21.98285809834373 44-45 21.51599696585699 26.109747979198445 30.54260421283 21.831650842114566 46-47 22.104182841707473 26.6169794848023 29.57042270985267 21.708414963637555 48-49 22.119090491972376 26.683642013092086 29.15786022875382 22.03940726618172 50-51 21.92327404108448 27.132880428216616 29.07144065285484 21.872404877844062 52-53 22.226023477342967 27.135121901588473 28.689637411133294 21.949217209935266 54-55 21.6168762861133 27.165446822741835 28.79183474423651 22.425842146908355 56-57 21.89046661911863 27.47907695789202 28.5052690595787 22.125187363410642 58-59 22.11476526211727 26.730937467527493 28.69789344601167 22.456403824343568 60-61 21.792219417372724 26.9654355505621 29.28551938475007 21.95682564731511 62-63 21.77422277149358 27.05921132516165 29.275209743463577 21.891356159881195 64-65 21.556257341031493 27.166919873297058 29.12088226559415 22.155940520077305 66-67 21.551786365600684 27.288005324451614 28.796939139902637 22.363269170045065 68-69 21.704472018399976 27.090669238049685 28.983046427372 22.22181231617834 70-71 21.889911565324603 27.289263657065916 28.562088240054877 22.258736537554604 72-73 21.903848844456316 27.160268884903527 28.528224011396446 22.40765825924371 74-75 21.58535883429853 27.86940714167371 28.246242065398825 22.298991958628932 76-77 21.780334221207106 27.498122620687987 28.336155495968324 22.385387662136583 78-79 21.73938472521852 27.57461914444618 28.43084682847379 22.2551493018615 80-81 21.89922067882349 27.824471072743528 28.057723868487127 22.218584379945856 82-83 21.85087056705121 27.69712379702537 28.09848623573216 22.353519400191253 84-85 21.742352375672063 27.610861305106386 28.05148349160974 22.595302827611814 86-87 21.95365507980078 27.460961170042648 27.984668687358088 22.600715062798486 88-89 21.81725915117584 27.4877878864864 27.809692298936135 22.885260663401624 90-91 21.866756641672314 27.47619155135969 27.961948459204528 22.695103347763467 92-93 21.9932906179945 27.110736601109753 28.096347997519754 22.799624783375997 94-95 21.898224776805232 26.901730703490824 28.27796195155302 22.922082568150923 96-97 22.003162147843767 26.58627314928174 28.29365490740287 23.116909795471617 98-99 21.905417544417503 26.83693310544934 27.911316030048887 23.346333320084263 100-101 22.236321142095175 26.17243152105867 28.52983733817197 23.061409998674183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6512.0 1 4005.0 2 2026.0 3 2486.0 4 1987.5 5 1868.0 6 3403.5 7 6975.5 8 10586.5 9 11118.0 10 8202.5 11 5658.5 12 5270.0 13 5182.0 14 5177.5 15 5091.0 16 4988.0 17 5053.5 18 5046.5 19 5083.5 20 5300.0 21 5732.5 22 6428.5 23 7341.5 24 8139.5 25 8925.5 26 10390.0 27 11979.5 28 13183.0 29 14795.0 30 16854.5 31 19501.5 32 23025.0 33 24464.0 34 24538.0 35 25391.0 36 27119.5 37 29574.5 38 31819.0 39 33507.0 40 34695.0 41 37337.0 42 39546.5 43 41296.5 44 43935.0 45 46928.5 46 49291.0 47 52497.5 48 60212.5 49 60851.5 50 55059.5 51 52605.0 52 51679.5 53 53351.5 54 55634.0 55 54178.0 56 49761.0 57 45792.0 58 43443.5 59 39300.0 60 32840.0 61 28024.0 62 24373.5 63 19180.0 64 13836.5 65 10391.0 66 7821.5 67 6509.0 68 4940.5 69 3268.0 70 2589.5 71 2009.5 72 1600.0 73 1309.5 74 1012.5 75 829.0 76 621.0 77 380.0 78 251.0 79 185.5 80 132.5 81 101.0 82 72.0 83 60.5 84 46.5 85 29.0 86 24.0 87 22.5 88 17.0 89 13.0 90 6.5 91 4.0 92 4.5 93 4.0 94 4.0 95 3.5 96 2.5 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3405484208981063 2 0.11566750527717173 3 0.10965633842742813 4 0.11611043336083705 5 0.13547271816106385 6 0.0867506289578788 7 0.09522953798804347 8 0.10029157322993282 9 0.0893449220193471 10-11 0.08773139828599487 12-13 0.09140137383636465 14-15 0.09307817301024049 16-17 0.08282755164541455 18-19 0.09747581612663186 20-21 0.10722023396726887 22-23 0.1148132868301029 24-25 0.12851241970346597 26-27 0.12810112934006246 28-29 0.12250125285372238 30-31 0.11342122713858334 32-33 0.10658747956203271 34-35 0.3294435810862115 36-37 0.4900366491351513 38-39 0.4642202694015156 40-41 0.4444466942378853 42-43 0.4758945881781229 44-45 0.4824752339925791 46-47 0.490574490379602 48-49 0.5008567494646897 50-51 0.48911915524755883 52-53 0.49430774137049543 54-55 0.4797227523298017 56-57 0.4584938420341283 58-59 0.43947957215678135 60-61 0.4006917271158042 62-63 0.4681749844342416 64-65 0.4995596029339556 66-67 0.5076904970412405 68-69 0.5198710193420367 70-71 0.5126259814020825 72-73 0.5044318118542741 74-75 0.5116135743537046 76-77 0.4885496762828463 78-79 0.46744731686822005 80-81 0.46953540640549934 82-83 0.4824119585520555 84-85 0.48190575502786653 86-87 0.4848797007324765 88-89 0.4983257318437451 90-91 0.5127525322831297 92-93 0.504115434651656 94-95 0.5096520356974725 96-97 0.5089243681314509 98-99 0.5033244916451108 100-101 0.49187163691033614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1580392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.699385645598454 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61731715010625 31.565506925501325 2 14.574649005470114 13.029557130894935 3 5.784358724311475 7.756718439914416 4 2.828100242880321 5.056573736036725 5 1.6619359949482955 3.7143758978247607 6 1.041555200024376 2.793412653424081 7 0.6918803872110583 2.1648639774001155 8 0.4907134216725749 1.7547670781450877 9 0.34395409739861454 1.3837083159603962 >10 1.7551333621183818 13.884829257913209 >50 0.1318593245927043 4.066396535782849 >100 0.06847723620987588 5.739733420894657 >500 0.006946856334366874 2.0915120451464406 >1k 0.0025519064085429335 1.912424266707953 >5k 1.4177257825238518E-4 0.3636888748968435 >10k+ 4.253177347571556E-4 2.721931443556233 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18401 1.164331381075075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14157 0.8957904114928448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10394 0.6576849288024743 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5739 0.36313775316503755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3205 0.20279778687819225 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3184 0.20146900262719628 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2477 0.15673326617699912 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2365 0.14964641683835403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 0.13964889723562254 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1665 0.1053536084718222 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1635 0.10345534525611366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.163772026180846E-5 0.0 12-13 0.0 0.0 0.0 1.2655088104723385E-4 0.0 14-15 0.0 0.0 0.0 1.8982632157085077E-4 0.0 16-17 0.0 0.0 0.0 3.4801492287989306E-4 0.0 18-19 0.0 0.0 0.0 6.011166849743607E-4 0.0 20-21 0.0 0.0 0.0 8.2258072680702E-4 0.0 22-23 0.0 0.0 0.0 0.001360421971257764 0.0 24-25 0.0 0.0 0.0 0.0025310176209446767 0.0 26-27 0.0 0.0 0.0 0.003764888711155207 0.0 28-29 0.0 0.0 0.0 0.007371588821001372 0.0 30-31 0.0 0.0 0.0 0.01714764438190019 0.0 32-33 0.0 0.0 0.0 0.03277667819123357 0.0 34-35 0.0 0.0 0.0 0.05454342973135779 0.0 36-37 0.0 0.0 0.0 0.08722519476180593 0.0 38-39 0.0 0.0 0.0 0.13670658925127438 0.0 40-41 0.0 0.0 0.0 0.19612222790295067 0.0 42-43 0.0 0.0 0.0 0.25686665080562293 0.0 44-45 0.0 0.0 0.0 0.3262165336195071 0.0 46-47 0.0 0.0 0.0 0.41410612050681095 0.0 48-49 0.0 0.0 0.0 0.510569529585065 0.0 50-51 0.0 0.0 0.0 0.6200676794111841 0.0 52-53 0.0 0.0 0.0 0.7251049106803882 0.0 54-55 0.0 0.0 0.0 0.8392221676647313 0.0 56-57 0.0 0.0 0.0 0.9792507175434956 0.0 58-59 0.0 0.0 0.0 1.1358574328394475 0.0 60-61 0.0 0.0 0.0 1.3201471533644817 3.163772026180846E-5 62-63 0.0 0.0 0.0 1.504247047567945 6.327544052361693E-5 64-65 0.0 0.0 0.0 1.6939468182577486 6.327544052361693E-5 66-67 0.0 0.0 0.0 1.9100640853661623 6.327544052361693E-5 68-69 0.0 0.0 0.0 2.163197485180892 6.327544052361693E-5 70-71 0.0 0.0 0.0 2.4464183569646014 6.327544052361693E-5 72-73 0.0 0.0 0.0 2.7399531255536598 6.327544052361693E-5 74-75 0.0 0.0 0.0 3.0392143215101064 6.327544052361693E-5 76-77 0.0 0.0 0.0 3.3611914006145307 6.327544052361693E-5 78-79 0.0 0.0 0.0 3.7409705946372798 6.327544052361693E-5 80-81 0.0 0.0 0.0 4.151818029957125 6.327544052361693E-5 82-83 0.0 0.0 0.0 4.587564351123012 1.2655088104723385E-4 84-85 0.0 0.0 0.0 5.024449630218326 1.2655088104723385E-4 86-87 0.0 6.327544052361693E-5 0.0 5.477849799290302 1.2655088104723385E-4 88-89 0.0 6.327544052361693E-5 0.0 5.962729500022779 1.2655088104723385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10575 0.0 34.116184 1 GTATCAA 16015 0.0 32.48709 1 GTGTAAG 500 0.0 30.381357 1 TATCAAC 19370 0.0 26.783756 2 ATCAACG 19720 0.0 26.23631 3 TCAACGC 19815 0.0 26.110521 4 CAACGCA 20085 0.0 25.759523 5 TAAGGTG 835 0.0 25.533659 5 AACGCAG 20505 0.0 25.360086 6 GTAAGGT 790 0.0 25.1889 4 CGACCGT 85 1.1525299E-7 25.082272 18-19 TAAGGTA 435 0.0 25.051031 4 GTACATG 12155 0.0 24.916838 1 TACATGG 12050 0.0 24.574234 2 GGTAAGG 755 0.0 23.846445 3 GAGTACT 11670 0.0 23.800425 12-13 ACGCAGA 22780 0.0 22.702679 7 CGCAGAG 22915 0.0 22.527594 8 ACATGGG 12535 0.0 22.527288 3 AGGTAAG 700 0.0 22.33587 2 >>END_MODULE