FastQCFastQC Report
Fri 27 May 2016
SRR939221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580392
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181461.1481961437415529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149590.9465373147927857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108100.684007512060299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58300.3688958182526867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.2135546117672071No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.21020101341945543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25690.16255460670517188No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.15799877498747145No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.1293982758707966No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.11073202091632962No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.10136725571883431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108850.035.43111
GTATCAA162450.031.6542531
TAAGGTA4800.031.633724
TATCAAC201300.025.5285552
ATCAACG205050.025.0616843
TCAACGC205600.024.9946384
TAGATAG2701.8189894E-1224.6040045
CAACGCA209050.024.5821485
GTGTAAG5900.024.2101381
AACGCAG213200.024.0813926
GAGTACT121650.023.63902312-13
AGTACTT123450.022.62165612-13
GTACATG120750.022.5546931
GTACTTT127200.022.43972614-15
TACATGG120050.022.055332
ACGCAGA236700.021.5903157
CGCAGAG238200.021.4145158
TAAGGTG8250.021.2808695
GTATTAG2751.2223609E-920.7767011
ACATGGG121750.020.5391853
Produced by FastQC (version 0.11.5)