FastQCFastQC Report
Fri 27 May 2016
SRR939220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559925
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179171.1485808612593553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139850.8965174607753578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97800.6269532189047551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56680.3633508021218969No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.19789412952545796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.19276567783707552No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.1575716781255509No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23110.14814814814814814No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.13757071654085934No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.10487683702742119No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.10295366764427775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102300.033.3733671
GTATCAA153150.031.8419931
TATCAAC189800.025.6564772
TCAACGC193600.025.1039544
ATCAACG193700.025.0176283
GTACATG119600.024.9330431
CAACGCA195800.024.7727055
TAAGGTA3650.024.658174
TACATGG119950.024.6029932
AACGCAG198600.024.483216
GAGTACT108450.024.25189212-13
AGTACTT110950.023.10799212-13
ACATGGG124200.022.5787683
GTACTTT117150.022.35053314-15
ACGCAGA220750.021.940787
CGCAGAG221750.021.7991358
GCAGAGT230200.020.8344149
CATGGGG45850.020.7661914
GTGTAAG6200.020.6784921
CATGGGA75450.020.4044194
Produced by FastQC (version 0.11.5)