##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1559925 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.173310255300734 33.0 31.0 34.0 31.0 34.0 2 32.24791704729394 34.0 31.0 34.0 30.0 34.0 3 32.280047438178116 34.0 31.0 34.0 30.0 34.0 4 35.74754940141353 37.0 35.0 37.0 35.0 37.0 5 35.79797105630078 37.0 35.0 37.0 35.0 37.0 6 35.903332532012755 37.0 37.0 37.0 35.0 37.0 7 35.844447008670286 37.0 37.0 37.0 35.0 37.0 8 35.841184031283554 37.0 37.0 37.0 35.0 37.0 9 37.50886164398929 39.0 38.0 39.0 35.0 39.0 10-11 37.42199657034793 39.0 38.0 39.0 35.0 39.0 12-13 37.319323685433595 39.0 37.5 39.0 34.5 39.0 14-15 38.56215298812443 40.0 38.0 41.0 34.0 41.0 16-17 38.49348173790406 40.0 38.0 41.0 34.0 41.0 18-19 38.45786944885171 40.0 38.0 41.0 34.0 41.0 20-21 38.46437681298781 40.0 38.0 41.0 34.0 41.0 22-23 38.471553440069236 40.0 38.0 41.0 34.0 41.0 24-25 38.40250204336747 40.0 38.0 41.0 34.0 41.0 26-27 38.265648669006524 40.0 38.0 41.0 34.0 41.0 28-29 38.10034040098081 40.0 38.0 41.0 34.0 41.0 30-31 37.79734089779957 40.0 37.5 41.0 33.0 41.0 32-33 37.49971152459253 40.0 36.5 41.0 33.0 41.0 34-35 37.15611423626136 40.0 36.0 41.0 32.5 41.0 36-37 36.86194881164158 40.0 35.0 41.0 31.0 41.0 38-39 36.63677708864208 40.0 35.0 41.0 31.0 41.0 40-41 36.38523230283507 39.0 35.0 41.0 30.0 41.0 42-43 36.09605782329278 39.0 35.0 41.0 30.0 41.0 44-45 35.71159030081574 39.0 35.0 41.0 28.5 41.0 46-47 35.34460599067263 39.0 35.0 41.0 25.5 41.0 48-49 35.063854351972054 39.0 35.0 41.0 24.0 41.0 50-51 33.85632450278058 38.0 34.0 40.0 21.5 40.5 52-53 33.9666628203279 38.0 33.5 39.5 22.0 40.5 54-55 34.43366155424139 38.0 35.0 40.5 22.0 41.0 56-57 34.297327115085665 38.0 35.0 41.0 21.5 41.0 58-59 34.066653845537445 38.0 34.5 40.0 20.5 41.0 60-61 33.74188534705194 37.0 34.0 40.0 19.5 41.0 62-63 33.34502492106992 37.0 34.0 40.0 17.5 41.0 64-65 32.953102873535585 36.0 33.5 39.5 13.5 41.0 66-67 32.55093962850778 35.5 33.0 39.0 9.0 41.0 68-69 32.13637001778932 35.0 33.0 39.0 6.5 40.5 70-71 31.694422167732423 35.0 32.0 38.0 2.0 40.0 72-73 31.257255316762024 35.0 32.0 37.0 2.0 39.5 74-75 30.82611119124317 35.0 31.0 37.0 2.0 39.0 76-77 30.402526082984757 35.0 31.0 36.0 2.0 39.0 78-79 29.994356138916935 35.0 31.0 36.0 2.0 37.5 80-81 29.57589531547991 35.0 30.0 35.0 2.0 37.0 82-83 29.213180761895604 34.0 30.0 35.0 2.0 37.0 84-85 28.86270012981393 34.0 29.5 35.0 2.0 36.0 86-87 28.469804317515266 34.0 29.0 35.0 2.0 36.0 88-89 28.047092648685034 34.0 29.0 35.0 2.0 35.5 90-91 27.6355161305832 34.0 27.0 35.0 2.0 35.0 92-93 27.230686731733897 34.0 26.5 35.0 2.0 35.0 94-95 26.75489302370306 34.0 25.0 35.0 2.0 35.0 96-97 26.16887061877975 33.0 24.5 35.0 2.0 35.0 98-99 25.483440870554674 33.0 20.5 35.0 2.0 35.0 100-101 22.787152587464142 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1647348144305596 1101 2 0.37964258491506087 1101 3 0.3428133016269328 1101 4 0.26548369831198215 1101 5 0.0846573240038282 1101 6 0.12370848689325697 1101 7 0.18353515774104778 1101 8 0.13672390454348005 1101 9 0.24345545100500487 1101 10-11 0.3206498202358219 1101 12-13 0.33914760275789746 1101 14-15 0.45856934062775423 1101 16-17 0.4774787802747582 1101 18-19 0.4130243629609893 1101 20-21 0.41204138487755415 1101 22-23 0.4378250349986317 1101 24-25 0.4250295288176389 1101 26-27 0.3757445173656322 1101 28-29 0.3335957340823654 1101 30-31 0.33806090496384655 1101 32-33 0.42526400685112975 1101 34-35 0.38360125282513735 1101 36-37 0.4199905820126517 1101 38-39 0.4026547148123356 1101 40-41 0.5349235701961703 1101 42-43 0.5121636354218353 1101 44-45 0.6000601702351389 1101 46-47 0.8107189333862692 1101 48-49 0.7427301091799663 1101 50-51 0.6508916167972103 1101 52-53 0.7850502714216887 1101 54-55 0.8785573434351335 1101 56-57 0.7546595403539413 1101 58-59 0.6477999156793572 1101 60-61 0.5401362267266876 1101 62-63 0.6333497215736728 1101 64-65 0.5588604908632462 1101 66-67 0.5793699031305266 1101 68-69 0.45426976145112974 1101 70-71 0.4265136068498485 1101 72-73 0.3746070717325303 1101 74-75 0.32080329346431924 1101 76-77 0.23863608180657891 1101 78-79 0.1552686538730086 1101 80-81 0.008077942245254377 1101 82-83 -0.035200926645767794 1101 84-85 -0.025937700129716745 1101 86-87 -0.2071230373088504 1101 88-89 -0.26723919947524877 1101 90-91 -0.25507838049330545 1101 92-93 -0.23320521814794049 1101 94-95 -0.29100915310953823 1101 96-97 -0.3237983951393488 1101 98-99 -0.3268674131855711 1101 100-101 -0.5811883817491186 1102 1 0.17046904401833984 1102 2 0.2424275316657969 1102 3 0.25415674970178515 1102 4 0.13909553998280444 1102 5 0.15529258077754093 1102 6 0.19498587718975813 1102 7 0.2324944232495909 1102 8 0.27958779728440675 1102 9 0.25736435912753564 1102 10-11 0.41272197600557803 1102 12-13 0.3846201060084127 1102 14-15 0.3561553056838491 1102 16-17 0.27046700099563026 1102 18-19 0.3401644095498284 1102 20-21 0.35321927863405733 1102 22-23 0.33510871911448703 1102 24-25 0.25661663468542173 1102 26-27 0.3868159125942583 1102 28-29 0.40135078291411475 1102 30-31 0.4009083003382088 1102 32-33 0.446105452243593 1102 34-35 0.5052300518305088 1102 36-37 0.38448167981049863 1102 38-39 0.30159411289184845 1102 40-41 0.11730793492801439 1102 42-43 0.16502073111112026 1102 44-45 0.1786620383427362 1102 46-47 0.30476525125146736 1102 48-49 0.29271206340632006 1102 50-51 0.22249263555798393 1102 52-53 0.3270197882959067 1102 54-55 0.2659969726371827 1102 56-57 0.16758185130596814 1102 58-59 0.03524722999005547 1102 60-61 0.06693220815490264 1102 62-63 0.1600779601923037 1102 64-65 0.031210323840220155 1102 66-67 0.03995525793207122 1102 68-69 -0.047181300915525526 1102 70-71 -0.04434787675183571 1102 72-73 -0.2825405916528787 1102 74-75 -0.5446099604448413 1102 76-77 -0.5423682081918493 1102 78-79 -0.5448865853217271 1102 80-81 -0.41832148757660903 1102 82-83 -0.5864284377407856 1102 84-85 -0.5774432983044164 1102 86-87 -0.5455416354994895 1102 88-89 -0.7781028121366624 1102 90-91 -0.8999144998208415 1102 92-93 -0.9162790225158162 1102 94-95 -0.9874570389646138 1102 96-97 -1.157744544729134 1102 98-99 -1.3107893119880636 1102 100-101 -1.4281522514916496 1103 1 0.10587976497178175 1103 2 0.20424104061739712 1103 3 0.25563940223047865 1103 4 0.23485109228724355 1103 5 0.19432084288605722 1103 6 0.2038850677357189 1103 7 0.1237138012342811 1103 8 0.1153059666561731 1103 9 0.19381282767194108 1103 10-11 0.1800667178296962 1103 12-13 0.14516762840651154 1103 14-15 0.18395989906803578 1103 16-17 0.3292918951464614 1103 18-19 0.24604381465460534 1103 20-21 0.34862720460187546 1103 22-23 0.4004226746087056 1103 24-25 0.26734211905861116 1103 26-27 0.2922329441011726 1103 28-29 0.26159576995455325 1103 30-31 0.19469322401108258 1103 32-33 0.1613960434048849 1103 34-35 0.0065161819327244075 1103 36-37 0.15752611789829274 1103 38-39 0.1657346111417155 1103 40-41 0.2644158890640469 1103 42-43 0.16748572282442353 1103 44-45 0.15893091377590451 1103 46-47 0.11820187167712959 1103 48-49 0.08642602064740146 1103 50-51 0.15701791380097774 1103 52-53 0.12382160416313326 1103 54-55 0.12073496457102095 1103 56-57 0.1372016677540131 1103 58-59 0.14130929177233753 1103 60-61 0.17347875230803567 1103 62-63 -0.04111241981655667 1103 64-65 -0.02810041742053926 1103 66-67 -0.06373434144605028 1103 68-69 0.035414891353283906 1103 70-71 -0.05632155148101603 1103 72-73 -0.22229827950102177 1103 74-75 -0.1579357094682301 1103 76-77 -0.21035366771587505 1103 78-79 -0.2375418871692574 1103 80-81 -0.47348151142132977 1103 82-83 -0.6311523018957494 1103 84-85 -0.6174151410078679 1103 86-87 -0.5949484653088852 1103 88-89 -0.6302477998321763 1103 90-91 -0.6257703549881803 1103 92-93 -0.7004258859087074 1103 94-95 -0.7443282272483813 1103 96-97 -0.8063376079241991 1103 98-99 -0.9681074250233941 1103 100-101 -1.248824718481341 1104 1 0.12002969670130881 1104 2 0.25396912664456295 1104 3 0.08567458859931776 1104 4 0.18682499024465216 1104 5 0.1411176516926247 1104 6 0.08189539218503938 1104 7 0.13886602421860061 1104 8 0.07062755987456626 1104 9 0.27750149307441774 1104 10-11 0.13343893949745222 1104 12-13 0.19254739061715753 1104 14-15 0.2936149686019931 1104 16-17 0.2109177410951517 1104 18-19 0.23599701274940088 1104 20-21 0.12581276226441673 1104 22-23 0.24646877363592523 1104 24-25 0.36095521976977096 1104 26-27 0.26758125092970175 1104 28-29 0.2706214345564675 1104 30-31 0.3863901859218899 1104 32-33 0.5480482101165052 1104 34-35 0.42370399050359 1104 36-37 0.4439430540722995 1104 38-39 0.24897973081146318 1104 40-41 0.6025118754396814 1104 42-43 0.6950633313829755 1104 44-45 0.7086884633436696 1104 46-47 1.0010014813781254 1104 48-49 0.9321307673034269 1104 50-51 0.7428475964866124 1104 52-53 0.7920669149477462 1104 54-55 0.7289906486454285 1104 56-57 0.8572247714168384 1104 58-59 0.8644741113492884 1104 60-61 0.866577754051427 1104 62-63 0.8868080806495513 1104 64-65 0.7408860474005294 1104 66-67 0.9703563022075627 1104 68-69 0.846412914376252 1104 70-71 0.8002223274286102 1104 72-73 0.8182483302275791 1104 74-75 0.5385802666721382 1104 76-77 0.4678203253742623 1104 78-79 0.39217732263450955 1104 80-81 0.5103687522109972 1104 82-83 0.3649481586910568 1104 84-85 0.48481936329327624 1104 86-87 0.4702386892244661 1104 88-89 0.3520520445153892 1104 90-91 0.3872804008710844 1104 92-93 0.33671569410152813 1104 94-95 0.1378611420938256 1104 96-97 0.1293360507149366 1104 98-99 0.18725860239946357 1104 100-101 0.2017537755760479 1105 1 0.19043915433556435 1105 2 0.22372482839219288 1105 3 0.33840182397711516 1105 4 0.25195972147549384 1105 5 0.24234813017208978 1105 6 0.1757450540088783 1105 7 0.21505965510586833 1105 8 0.27616368592157414 1105 9 0.335924056639719 1105 10-11 0.36022624030218964 1105 12-13 0.2833339519022715 1105 14-15 0.22636490312368807 1105 16-17 0.2789232464078779 1105 18-19 0.3082159824547759 1105 20-21 0.18707066459646882 1105 22-23 0.27791222349495115 1105 24-25 0.3956635973972453 1105 26-27 0.41703380098850573 1105 28-29 0.339228826671345 1105 30-31 0.34818680775517663 1105 32-33 0.33050182323476207 1105 34-35 0.3363356532415551 1105 36-37 0.6153660130751817 1105 38-39 0.47577755949969003 1105 40-41 0.4465346053969057 1105 42-43 0.3907813073588571 1105 44-45 0.4151636252419735 1105 46-47 0.39103157571823033 1105 48-49 0.6027063600746274 1105 50-51 0.5099764629160219 1105 52-53 0.6089051090819169 1105 54-55 0.6219677800282781 1105 56-57 0.5150359474071067 1105 58-59 0.6360847748113727 1105 60-61 0.6412846423685608 1105 62-63 0.5932462665668368 1105 64-65 0.6595355510493377 1105 66-67 0.5998510665113628 1105 68-69 0.5310119047265545 1105 70-71 0.4939415462083012 1105 72-73 0.5026814422185772 1105 74-75 0.6676118041026164 1105 76-77 0.6486025727949318 1105 78-79 0.5604365242614548 1105 80-81 0.5758539849285818 1105 82-83 0.44061565218174437 1105 84-85 0.2824200329693767 1105 86-87 0.18442488383110955 1105 88-89 0.15532082394852864 1105 90-91 0.3054379185502256 1105 92-93 0.3026803306841259 1105 94-95 0.18777281058360984 1105 96-97 0.23561503449172605 1105 98-99 -0.03150824863355339 1105 100-101 0.010226154004673305 1106 1 0.18043880789181088 1106 2 0.19531446081536785 1106 3 0.3299886848502567 1106 4 0.28487534262439596 1106 5 0.2830003352865944 1106 6 0.29459258411236533 1106 7 0.3272703127056076 1106 8 0.3185258384261189 1106 9 0.3505227015497212 1106 10-11 0.4318374017301281 1106 12-13 0.3571158309142177 1106 14-15 0.5282980592757909 1106 16-17 0.577526583156164 1106 18-19 0.49638864012380424 1106 20-21 0.4845713203650064 1106 22-23 0.5076385824205829 1106 24-25 0.3681096882743944 1106 26-27 0.39391041257061943 1106 28-29 0.2842363247040396 1106 30-31 0.40431564523841246 1106 32-33 0.48286061028567673 1106 34-35 0.3605414309335444 1106 36-37 0.20049986409446774 1106 38-39 0.45602828579427523 1106 40-41 0.6275918032608274 1106 42-43 0.632259038449213 1106 44-45 0.5569994306989585 1106 46-47 0.6803336400280884 1106 48-49 0.6146415948892496 1106 50-51 0.4595352420482257 1106 52-53 0.4892798127487694 1106 54-55 0.36069128326752775 1106 56-57 0.46823411818407834 1106 58-59 0.4699555908843891 1106 60-61 0.6299752334510984 1106 62-63 0.5216360949781986 1106 64-65 0.6459052164341585 1106 66-67 0.647523954318018 1106 68-69 0.6328940841547208 1106 70-71 0.5105357071462713 1106 72-73 0.6369704653956667 1106 74-75 0.5338179480351499 1106 76-77 0.5383849566249133 1106 78-79 0.43951627066462606 1106 80-81 0.39504226721095037 1106 82-83 0.4594193818962218 1106 84-85 0.48579587990062834 1106 86-87 0.5195837282927087 1106 88-89 0.3559543211002669 1106 90-91 0.3351546265426819 1106 92-93 0.2755473507234214 1106 94-95 0.4037134301240286 1106 96-97 0.5818199906170989 1106 98-99 0.4695459302205087 1106 100-101 0.10699705074958743 1107 1 0.03612779329048266 1107 2 0.14774747704814928 1107 3 0.030522140130706532 1107 4 0.10407288745612675 1107 5 0.14602563804694313 1107 6 0.20109434962022021 1107 7 0.14520053283791157 1107 8 0.12745168329340828 1107 9 0.26737032405618777 1107 10-11 0.24945411824680974 1107 12-13 0.2313942259278221 1107 14-15 0.3133916872850975 1107 16-17 0.18820880885593994 1107 18-19 0.30206393499308604 1107 20-21 0.37793873424301694 1107 22-23 0.28904765163571255 1107 24-25 0.31144325612142154 1107 26-27 0.29631714108551677 1107 28-29 0.35693565025531626 1107 30-31 0.45614475700827484 1107 32-33 0.331957463175236 1107 34-35 0.5493388225074085 1107 36-37 0.32140839234354246 1107 38-39 0.4110061717863971 1107 40-41 0.37513867906301357 1107 42-43 0.22291553736252467 1107 44-45 0.2365426423830641 1107 46-47 0.3023143553962768 1107 48-49 0.2260917737217909 1107 50-51 0.3177488941680693 1107 52-53 0.3266211119419822 1107 54-55 0.18457189234396054 1107 56-57 0.28879239705636195 1107 58-59 0.22613154080747222 1107 60-61 -0.033960299367187474 1107 62-63 0.04841130028859908 1107 64-65 0.09639517576187728 1107 66-67 -0.04751641372904203 1107 68-69 -0.06921806029558297 1107 70-71 0.009304272549922388 1107 72-73 0.05370613930246648 1107 74-75 -0.0923438455002561 1107 76-77 -0.005811209646562787 1107 78-79 -0.19485508684643804 1107 80-81 -0.05468401727445027 1107 82-83 -0.20720885938414213 1107 84-85 -0.11201249452013684 1107 86-87 -0.15441553203840996 1107 88-89 -0.16509581856214695 1107 90-91 -0.04677525322784959 1107 92-93 -0.07096113645406632 1107 94-95 0.0848137018430073 1107 96-97 0.11302821786467376 1107 98-99 0.06710616379615075 1107 100-101 0.07425933467215273 1108 1 0.07736777327745159 1108 2 0.22837551184778704 1108 3 0.16001382356488847 1108 4 0.11671421148363947 1108 5 0.13673901618162887 1108 6 0.2344173044937179 1108 7 0.11733346831028513 1108 8 0.13686428646309423 1108 9 0.22641470187052448 1108 10-11 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-1.5736485681744696 2316 54-55 -1.4643707163145905 2316 56-57 -1.5736220864855568 2316 58-59 -1.3603420617349613 2316 60-61 -1.286751748521219 2316 62-63 -1.3616464483156747 2316 64-65 -1.5871452722563788 2316 66-67 -1.7050419953475995 2316 68-69 -1.5254800246012863 2316 70-71 -1.4276601664674438 2316 72-73 -1.7165278678534541 2316 74-75 -1.3751360411736435 2316 76-77 -1.3518255961409587 2316 78-79 -1.0879875087151873 2316 80-81 -1.0772542300857886 2316 82-83 -1.0283688171524794 2316 84-85 -1.0310971284857047 2316 86-87 -1.0130314318341824 2316 88-89 -1.1152069966230123 2316 90-91 -1.4056511017270772 2316 92-93 -1.3069812284892386 2316 94-95 -1.2843767779023239 2316 96-97 -1.3031023323734132 2316 98-99 -1.3619824960416373 2316 100-101 -1.3983080786916098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5436.0 3 1263.0 4 1287.0 5 1399.0 6 1607.0 7 1937.0 8 2397.0 9 2954.0 10 3610.0 11 4769.0 12 6774.0 13 11008.0 14 9898.0 15 10996.0 16 14889.0 17 22963.0 18 18741.0 19 11503.0 20 10808.0 21 10843.0 22 11457.0 23 12141.0 24 13155.0 25 14316.0 26 16201.0 27 18951.0 28 21897.0 29 25589.0 30 30969.0 31 38024.0 32 47426.0 33 62520.0 34 89108.0 35 145435.0 36 217499.0 37 318583.0 38 276646.0 39 44917.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.699617038720206 18.441345660683822 14.889606632933125 20.96943066766284 2 15.971419072692289 20.808329621552378 39.52565364783563 23.6945976579197 3 19.40922122690826 26.00818058647861 29.920177346591544 24.66242084002159 4 12.820644492059069 17.012821286390846 36.081965340033044 34.084568881517036 5 13.452724527681776 37.96069963179976 32.563574880219385 16.02300096029908 6 30.794240385941613 35.770423759530665 17.259468111381622 16.175867743146107 7 28.085718758501045 34.790444441022636 19.582492852706732 17.541343947769583 8 27.717576802757225 32.806344904752 19.207040883626274 20.26903740886449 9 28.21921798232614 16.602959159189215 19.86262788015033 35.31519497833431 10-11 25.616278845968782 26.918381225500575 25.371236471184506 22.09410345734614 12-13 27.436817198450967 24.72171466113026 26.6357724495269 21.205695690891872 14-15 23.968454270817762 25.236807884251068 26.613870189824816 24.180867655106358 16-17 21.65817359232345 28.84278199883109 27.08797789982941 22.411066509016045 18-19 21.63360684373784 25.991530021073896 31.223366901637313 21.151496233550958 20-21 22.91176441338655 24.723845746921345 31.41083200280766 20.95355783688445 22-23 22.30988778579715 25.002919571265775 31.520365132490785 21.16682751044629 24-25 21.931059090649473 25.445039349057552 31.573326967148045 21.050574593144926 26-27 21.496336827081297 25.470314694021095 31.674412724327627 21.358935754569984 28-29 21.260821017640904 26.01934146607543 31.613863736997132 21.105973779286536 30-31 21.348947620528033 25.675344871119886 31.42017569441838 21.5555318139337 32-33 21.217921365437366 25.81658351914532 31.320938738189575 21.64455637722774 34-35 21.950278997218067 25.904996221075145 30.88401112772766 21.260713653979128 36-37 22.139914884403122 25.452031612891584 30.97881003137059 21.429243471334704 38-39 21.61360780902677 25.795496279114722 30.93511806964982 21.655777842208693 40-41 21.67098657521858 25.838517438302816 31.060121017557464 21.430374968921136 42-43 21.58179076974621 26.013549646600993 30.442151449354732 21.962508134298066 44-45 21.5421785653991 26.167406189308345 30.460944054457045 21.829471190835502 46-47 22.100887687678785 26.68498121883162 29.469504079858062 21.74462701363153 48-49 22.120243580242935 26.699197731739538 29.07146309243806 22.10909559557947 50-51 21.993022517010353 27.12245199114089 29.020607030709815 21.863918461138937 52-53 22.271392085645992 27.11752513811505 28.648027082051954 21.963055694187005 54-55 21.630122625346093 27.186601742438448 28.74124576423536 22.4420298679801 56-57 21.915225839615513 27.567384068739702 28.356803338299812 22.160586753344965 58-59 22.07347196413677 26.783355286252103 28.671359975266736 22.47181277434439 60-61 21.77565605109483 26.99445133637236 29.213015507803476 22.016877104729335 62-63 21.794204821058525 27.048044688418372 29.26697116519813 21.89077932532497 64-65 21.555972277266005 27.125941248682977 29.13906153841197 22.17902493563905 66-67 21.510932087327802 27.23821799726094 28.790042697172318 22.460807218238944 68-69 21.713201147497077 27.082755217705053 28.990351471741388 22.213692163056486 70-71 21.905729357348747 27.280611403024785 28.54465634684501 22.269002892781455 72-73 21.848406427844324 27.258241278428102 28.434984440811768 22.458367852915806 74-75 21.597115254477607 27.92222272349366 28.21002055929003 22.270641462738705 76-77 21.78804228239358 27.512550793543916 28.291033435384875 22.408373488677626 78-79 21.759522442207977 27.64261238335389 28.307615602565967 22.29024957187217 80-81 21.934200426535384 27.855446958640368 27.988252077838037 22.222100536986208 82-83 21.878128926258817 27.69630488708482 28.03830417834477 22.387262008311588 84-85 21.76310678610855 27.569163944391168 28.088213645018712 22.57951562448157 86-87 21.99851559016888 27.426597737821268 27.96279569005729 22.612090981952566 88-89 21.880894628882622 27.506974085942904 27.76886273188292 22.84326855329155 90-91 21.944420456200543 27.45101135152344 27.871135010562703 22.733433181713313 92-93 22.032265226698616 27.093593519241484 28.146919793233927 22.727221460825973 94-95 21.88424000886783 26.842942727734396 28.249905263727737 23.022911999670033 96-97 21.980752840108078 26.531273815258842 28.33637577880428 23.151597565828798 98-99 21.96986443507094 26.812467977739896 27.86953285363048 23.348134733558684 100-101 22.339030643310423 26.030641377457364 28.50277606137554 23.127551917856675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6138.0 1 3834.0 2 2001.0 3 2432.0 4 1976.5 5 1857.0 6 3282.5 7 6650.5 8 10179.0 9 10873.0 10 8171.0 11 5611.0 12 5177.0 13 5152.0 14 5203.0 15 5129.5 16 4997.5 17 5043.0 18 5099.0 19 5121.0 20 5275.0 21 5742.5 22 6349.0 23 7061.5 24 7866.0 25 8777.0 26 10239.0 27 11807.5 28 13089.0 29 14555.0 30 16634.5 31 19380.0 32 22678.5 33 24038.0 34 24144.5 35 24781.5 36 26436.5 37 29032.0 38 31131.0 39 32923.5 40 34185.0 41 36868.0 42 39223.0 43 40786.5 44 43327.5 45 46458.0 46 48719.0 47 52076.5 48 59853.5 49 60119.0 50 54468.0 51 52029.0 52 50932.0 53 52675.0 54 54931.5 55 53599.5 56 49599.0 57 45580.5 58 42736.5 59 38698.5 60 32394.5 61 27686.5 62 24101.5 63 18706.0 64 13556.5 65 10336.0 66 7812.0 67 6473.0 68 4888.0 69 3212.5 70 2459.5 71 1922.0 72 1584.5 73 1323.5 74 1009.0 75 798.0 76 621.0 77 393.5 78 243.5 79 180.5 80 137.5 81 95.5 82 73.0 83 66.5 84 49.5 85 29.5 86 23.0 87 18.5 88 13.5 89 11.5 90 9.5 91 8.5 92 5.5 93 3.0 94 3.0 95 2.5 96 2.0 97 3.5 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.383479974998798 2 0.11686459284901518 3 0.11833902270942513 4 0.13122425757648604 5 0.13314742695962947 6 0.07308043655944997 7 0.08288860041348142 8 0.08058079715370932 9 0.07942689552382326 10-11 0.07788836001730852 12-13 0.08160648749138581 14-15 0.07513181723480296 16-17 0.07718319791015593 18-19 0.0872798371716589 20-21 0.0852925621424107 22-23 0.09468403929676106 24-25 0.10846675320928892 26-27 0.1098129717774893 28-29 0.1035306184592208 30-31 0.0949084090581278 32-33 0.09295318685193199 34-35 0.33655464205009855 36-37 0.5226533326922769 38-39 0.5048319630751479 40-41 0.47585621103578696 42-43 0.5035498501530522 44-45 0.5143195986986554 46-47 0.5110181579242592 48-49 0.517653092296104 50-51 0.5177171979422087 52-53 0.5159542926743272 54-55 0.5104091542862638 56-57 0.4819462474157411 58-59 0.4718175553311858 60-61 0.42168694007724733 62-63 0.49717133836562655 64-65 0.521178902831867 66-67 0.5320768626696796 68-69 0.5480071157267177 70-71 0.5434235620302258 72-73 0.5301216404634838 74-75 0.5330384473612514 76-77 0.508453932080068 78-79 0.48249114540763177 80-81 0.49044024552462456 82-83 0.5048319630751479 84-85 0.49755597224225523 86-87 0.4995753000945558 88-89 0.5083257207878584 90-91 0.5281664182572879 92-93 0.5331346058304085 94-95 0.5288074747183358 96-97 0.5299934291712742 98-99 0.5301536932865362 100-101 0.506819238104396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1559925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.83283318853524 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71008369878709 31.701333872150865 2 14.456286434784621 12.962325565127674 3 5.853036157625975 7.872245811039319 4 2.84254684650036 5.097577143989902 5 1.6517626368634317 3.7026599382776677 6 1.0328434589503026 2.77831791029932 7 0.679353588067221 2.1320142262895425 8 0.4786492439502725 1.7167361367872864 9 0.3432435560204133 1.3849722981092558 >10 1.7469405367400868 13.989581576395466 >50 0.1256851019422989 3.8922740363107806 >100 0.06924643448873453 5.711887054772771 >500 0.007168267554985611 2.119524113417823 >1k 0.00258057631979482 1.8951409656757796 >5k 2.8673070219942444E-4 0.9929163180518717 >10k+ 2.8673070219942444E-4 2.050493033304688 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17917 1.1485808612593553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13985 0.8965174607753578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9780 0.6269532189047551 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5668 0.3633508021218969 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.19789412952545796 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3007 0.19276567783707552 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2458 0.1575716781255509 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2311 0.14814814814814814 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.13757071654085934 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1636 0.10487683702742119 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1606 0.10295366764427775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.410564610478068E-5 0.0 5 0.0 0.0 0.0 6.410564610478068E-5 0.0 6 0.0 0.0 0.0 6.410564610478068E-5 0.0 7 0.0 0.0 0.0 6.410564610478068E-5 0.0 8 0.0 0.0 0.0 6.410564610478068E-5 0.0 9 0.0 0.0 0.0 6.410564610478068E-5 0.0 10-11 0.0 0.0 0.0 1.2821129220956135E-4 0.0 12-13 0.0 0.0 0.0 3.846338766286841E-4 0.0 14-15 0.0 0.0 0.0 5.128451688382454E-4 0.0 16-17 0.0 0.0 0.0 6.731092841001971E-4 0.0 18-19 0.0 0.0 0.0 0.0011539016298860523 0.0 20-21 0.0 0.0 0.0 0.0012500600990432232 0.0 22-23 0.0 0.0 0.0 0.0017629052678814687 0.0 24-25 0.0 0.0 0.0 0.0026924371364007884 0.0 26-27 0.0 0.0 0.0 0.003910444412391622 0.0 28-29 0.0 0.0 0.0 0.007756783178678462 0.0 30-31 0.0 0.0 0.0 0.018013686555443373 0.0 32-33 0.0 0.0 0.0 0.03211692869849512 0.0 34-35 0.0 0.0 0.0 0.05381668990496338 0.0 36-37 0.0 0.0 0.0 0.08782473516354952 0.0 38-39 0.0 0.0 0.0 0.13856435405548345 0.0 40-41 0.0 0.0 0.0 0.19600301296536693 0.0 42-43 0.0 0.0 0.0 0.2532173021138837 0.0 44-45 0.0 0.0 0.0 0.3237335128291424 0.0 46-47 0.0 0.0 0.0 0.41457121335961666 0.0 48-49 0.0 0.0 0.0 0.5143837043447601 0.0 50-51 0.0 0.0 0.0 0.6260557398592881 0.0 52-53 0.0 0.0 0.0 0.733945542253634 0.0 54-55 0.0 0.0 0.0 0.8504575540490729 0.0 56-57 0.0 0.0 0.0 0.9960414763530299 0.0 58-59 0.0 0.0 0.0 1.1669150760453229 0.0 60-61 0.0 0.0 0.0 1.3573408977995736 0.0 62-63 0.0 0.0 0.0 1.5432793243264902 0.0 64-65 0.0 0.0 0.0 1.7297305960222447 0.0 66-67 0.0 0.0 0.0 1.9479141625398657 0.0 68-69 0.0 0.0 0.0 2.1997852460855487 0.0 70-71 0.0 0.0 0.0 2.4848310014904564 0.0 72-73 0.0 0.0 0.0 2.7840441046845203 0.0 74-75 0.0 0.0 0.0 3.089539561196853 0.0 76-77 0.0 0.0 0.0 3.414747503886405 0.0 78-79 0.0 0.0 0.0 3.784957610141513 0.0 80-81 0.0 0.0 0.0 4.198919819863134 0.0 82-83 0.0 0.0 0.0 4.629517444748947 3.205282305239034E-5 84-85 0.0 0.0 0.0 5.063544721701364 6.410564610478068E-5 86-87 0.0 0.0 0.0 5.510489286343895 6.410564610478068E-5 88-89 0.0 0.0 0.0 5.9915380547141694 6.410564610478068E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10230 0.0 33.373367 1 GTATCAA 15315 0.0 31.841993 1 TATCAAC 18980 0.0 25.656477 2 TCAACGC 19360 0.0 25.103954 4 ATCAACG 19370 0.0 25.017628 3 GTACATG 11960 0.0 24.933043 1 CAACGCA 19580 0.0 24.772705 5 TAAGGTA 365 0.0 24.65817 4 TACATGG 11995 0.0 24.602993 2 AACGCAG 19860 0.0 24.48321 6 GAGTACT 10845 0.0 24.251892 12-13 AGTACTT 11095 0.0 23.107992 12-13 ACATGGG 12420 0.0 22.578768 3 GTACTTT 11715 0.0 22.350533 14-15 ACGCAGA 22075 0.0 21.94078 7 CGCAGAG 22175 0.0 21.799135 8 GCAGAGT 23020 0.0 20.834414 9 CATGGGG 4585 0.0 20.766191 4 GTGTAAG 620 0.0 20.678492 1 CATGGGA 7545 0.0 20.404419 4 >>END_MODULE