FastQCFastQC Report
Fri 27 May 2016
SRR939220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559925
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174971.1216564898953476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146570.9395964549577704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104320.6687501001650721No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57830.3707229514239467No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32330.20725355385675595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.20622786351907943No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25480.16334118627498118No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.15917431927817044No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.12718560187188488No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.108146224978765No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.10397935798195426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111950.033.998751
GTATCAA162750.029.357571
ATCACGC1151.2914736E-424.7558613
TATCAAC201250.023.7184722
GAGTACT115650.023.6138112-13
ATCAACG202150.023.4485683
TCAACGC203800.023.305294
CAACGCA205600.023.1012545
GTACATG118150.022.618791
AACGCAG210100.022.6064626
TACATGG119950.021.9541852
AGTACTT117800.021.91391612-13
TATCACG1302.9637222E-421.8994142
GTACTTT123550.021.89265614-15
ACGCAGA233050.020.3802557
CGCAGAG234000.020.2975128
ACATGGG121800.020.257263
ACTTTTT136250.019.79850816-17
TACTTTT130700.019.5694914-15
AGAGTAC201400.019.509110-11
Produced by FastQC (version 0.11.5)