FastQCFastQC Report
Fri 27 May 2016
SRR939219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191174
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT163220.7448974841797137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT96730.4414528467387802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86980.39695615227270864No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73030.33329165095971386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72260.3297775530377779No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63470.2896620715652888No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48240.2201559529275174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.21362977107249356No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40600.18528879952025717No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39630.18086194889132493No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36230.16534515287238713No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34560.15772366776896768No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32380.14777466326270758No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31670.14453439115287056No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26740.12203503692541076No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23300.1063356903650737No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22300.101771926830092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG75050.023.5498892
GTACATG77100.023.3356691
ACATGGG75950.021.7111193
GAGTACT91350.021.25897412-13
TAAGGTG12300.021.187925
GTACTTT94600.020.30492814-15
GTAAGGT11050.020.1542364
GTATAGG4950.020.139541
AGTACTT93950.019.6875312-13
AGAGTAC127300.019.6450310-11
AGGTAAG10700.019.0421262
GTGTAGC12300.018.5255681
GGTAAGG11450.018.2086563
CATGGGG43500.018.0821294
ACTTTTT111900.017.42112516-17
TACTTTT107150.017.0646314-15
ACGTGCG1700.001817771116.7237285
CCTACAC9100.016.6624683
GTATAGC10450.016.353911
GCACCGT3251.9760228E-716.032136
Produced by FastQC (version 0.11.5)