##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939219_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12078958585671 33.0 31.0 34.0 30.0 34.0 2 32.21316381081557 34.0 31.0 34.0 30.0 34.0 3 32.2731800395587 34.0 31.0 34.0 30.0 34.0 4 35.7124755952745 37.0 35.0 37.0 35.0 37.0 5 35.765688165339675 37.0 35.0 37.0 35.0 37.0 6 35.857774416819474 37.0 36.0 37.0 35.0 37.0 7 35.78779412315042 37.0 36.0 37.0 35.0 37.0 8 35.777755668878875 37.0 36.0 37.0 35.0 37.0 9 37.466133679935965 39.0 38.0 39.0 35.0 39.0 10-11 37.37924190411168 39.0 37.5 39.0 35.0 39.0 12-13 37.294328063403455 39.0 37.0 39.0 34.0 39.0 14-15 38.58594045931542 40.0 38.0 41.0 34.0 41.0 16-17 38.536175812600916 40.0 38.0 41.0 34.0 41.0 18-19 38.48975389448761 40.0 38.0 41.0 34.0 41.0 20-21 38.392504657320686 40.0 38.0 41.0 34.0 41.0 22-23 38.30987292656813 40.0 38.0 41.0 34.0 41.0 24-25 38.33935346987505 40.0 38.0 41.0 34.0 41.0 26-27 38.24386265079816 40.0 38.0 41.0 34.0 41.0 28-29 38.13049054981485 40.0 38.0 41.0 33.5 41.0 30-31 37.95122181077359 40.0 38.0 41.0 33.0 41.0 32-33 37.71620875384612 40.0 37.5 41.0 33.0 41.0 34-35 37.378851245953086 40.0 37.0 41.0 31.5 41.0 36-37 37.09918906485747 40.0 37.0 41.0 31.0 41.0 38-39 36.83916544281741 40.0 36.5 41.0 30.5 41.0 40-41 36.69660100019442 40.0 36.0 41.0 30.0 41.0 42-43 36.475421166917826 40.0 36.0 41.0 30.0 41.0 44-45 36.18747803688799 39.0 35.5 41.0 29.5 41.0 46-47 35.789912622183365 39.0 35.0 41.0 27.5 41.0 48-49 35.69846735129205 39.0 35.0 41.0 27.0 41.0 50-51 34.670366205513574 38.0 34.0 40.0 25.5 40.5 52-53 34.83245100571657 38.5 34.5 39.5 26.0 40.5 54-55 35.33727170913857 39.0 35.0 41.0 26.0 41.0 56-57 35.18935511282993 39.0 35.0 41.0 26.0 41.0 58-59 34.985761514147214 39.0 35.0 41.0 26.0 41.0 60-61 34.66114124209214 38.0 35.0 40.5 25.0 41.0 62-63 34.31640800776205 38.0 34.0 40.0 23.5 41.0 64-65 33.95293869861544 37.0 34.0 40.0 22.5 41.0 66-67 33.54832500750739 37.0 34.0 39.5 22.0 41.0 68-69 33.13749204764204 36.0 33.0 39.0 20.5 41.0 70-71 32.6997034466455 35.5 33.0 39.0 19.5 41.0 72-73 32.25304768128866 35.0 32.5 38.0 18.0 40.0 74-75 31.82449180211156 35.0 32.0 37.0 14.5 39.5 76-77 31.406640914870295 35.0 32.0 37.0 11.0 39.0 78-79 30.98404531086988 35.0 31.0 36.0 7.0 39.0 80-81 30.55586594218442 35.0 31.0 36.0 4.5 37.5 82-83 30.122586339560435 34.5 31.0 35.5 2.0 37.0 84-85 29.748064279696635 34.0 30.0 35.0 2.0 37.0 86-87 29.41383386257778 34.0 30.0 35.0 2.0 36.0 88-89 29.006772625085915 34.0 29.5 35.0 2.0 36.0 90-91 28.61397862515711 34.0 29.0 35.0 2.0 36.0 92-93 28.210334961988416 34.0 29.0 35.0 2.0 35.0 94-95 27.753954272914886 34.0 28.0 35.0 2.0 35.0 96-97 27.194103252411722 34.0 26.5 35.0 2.0 35.0 98-99 26.525230766703146 34.0 25.0 35.0 2.0 35.0 100-101 23.967738755571215 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29803176407027365 1101 2 0.33540771234267197 1101 3 0.3275394460452361 1101 4 0.26743021354725016 1101 5 0.3524177445081733 1101 6 0.3953494034954872 1101 7 0.23570717627114135 1101 8 0.39399210852850075 1101 9 0.5040362571316237 1101 10-11 0.4746639938982682 1101 12-13 0.52846875146043 1101 14-15 0.5301271118816189 1101 16-17 0.6206112420200895 1101 18-19 0.6753984892100675 1101 20-21 0.7314909552987459 1101 22-23 0.6272957843189602 1101 24-25 0.6548991990853139 1101 26-27 0.6884024824945953 1101 28-29 0.6265106787416812 1101 30-31 0.6072542939239369 1101 32-33 0.6261094444246496 1101 34-35 0.6704746589406625 1101 36-37 0.7053481830376569 1101 38-39 0.7669890574521006 1101 40-41 0.5732719502132468 1101 42-43 0.6290736698527155 1101 44-45 0.8142859807923628 1101 46-47 0.8130424910058665 1101 48-49 0.8704807167783457 1101 50-51 0.9155330776544517 1101 52-53 0.9448562361484889 1101 54-55 0.8661716342180625 1101 56-57 0.890108838139021 1101 58-59 0.9973038441557875 1101 60-61 0.8634119758581207 1101 62-63 1.0558130445334086 1101 64-65 1.053837406348805 1101 66-67 0.9036155631752223 1101 68-69 0.8797008944013669 1101 70-71 0.9417409450865009 1101 72-73 0.7386095242127126 1101 74-75 0.849828183976701 1101 76-77 0.744231219231807 1101 78-79 0.8828139526789478 1101 80-81 0.770135005794419 1101 82-83 0.8120431441282427 1101 84-85 0.7146246201391477 1101 86-87 0.6884186589008365 1101 88-89 0.7478731210398699 1101 90-91 0.45591395304367666 1101 92-93 0.45914893967097825 1101 94-95 0.26375165980146775 1101 96-97 0.2613335187824113 1101 98-99 0.44287825482785337 1101 100-101 0.3255364845448234 1102 1 0.06930488574528226 1102 2 0.12157874095834842 1102 3 0.1522899356207219 1102 4 0.1447527119090708 1102 5 0.16171541230953324 1102 6 0.25773642814840514 1102 7 0.19447818481543777 1102 8 0.25626884788328397 1102 9 0.22150464335449982 1102 10-11 0.2297157850549425 1102 12-13 0.1649166203244974 1102 14-15 0.2726422016474146 1102 16-17 0.30732049509077086 1102 18-19 0.25625814030065897 1102 20-21 0.45303090836175386 1102 22-23 0.39119146355028533 1102 24-25 0.2900365244065526 1102 26-27 0.40121661446880097 1102 28-29 0.42552216290272327 1102 30-31 0.4336963614056586 1102 32-33 0.31260416441536876 1102 34-35 0.25600520099156654 1102 36-37 0.34379272563273133 1102 38-39 0.11241227124619257 1102 40-41 0.07598266087453709 1102 42-43 0.09654084857231737 1102 44-45 0.06052098561432473 1102 46-47 0.048017393721352164 1102 48-49 -0.022959803489193575 1102 50-51 0.07014139311226586 1102 52-53 1.5009145954536507E-4 1102 54-55 -0.07221430421859054 1102 56-57 -0.1443232516090589 1102 58-59 -0.05111220672893779 1102 60-61 0.01609539694563722 1102 62-63 -0.19464688004325126 1102 64-65 -0.2522796978544335 1102 66-67 -0.27923464362984163 1102 68-69 -0.229859901777985 1102 70-71 -0.332112476897251 1102 72-73 -0.3591091532867736 1102 74-75 -0.3495944684625343 1102 76-77 -0.4012742657846857 1102 78-79 -0.3352466468115409 1102 80-81 -0.2639106829090956 1102 82-83 -0.37796192201878753 1102 84-85 -0.4028568607857892 1102 86-87 -0.5109185291406959 1102 88-89 -0.2391003337450357 1102 90-91 -0.3805431465120179 1102 92-93 -0.3412160214822997 1102 94-95 -0.49733214682841975 1102 96-97 -0.5942044131884643 1102 98-99 -0.7082682829382883 1102 100-101 -0.6837659864361889 1103 1 0.09950330243078298 1103 2 0.15337481072130998 1103 3 0.2718794753626739 1103 4 0.15284145203952448 1103 5 0.20042383041842982 1103 6 0.13655708491831575 1103 7 0.1918695046349157 1103 8 0.13408423391562252 1103 9 0.17206949980121067 1103 10-11 0.28690370631623097 1103 12-13 0.3005305733358128 1103 14-15 0.41654348939870545 1103 16-17 0.398610957107131 1103 18-19 0.4073475586356281 1103 20-21 0.4540364407523043 1103 22-23 0.45734361580301197 1103 24-25 0.40479752011125925 1103 26-27 0.3226364691153307 1103 28-29 0.31323378783051226 1103 30-31 0.2993953830328522 1103 32-33 0.21259019214640063 1103 34-35 0.15342833781303966 1103 36-37 0.017198788027549483 1103 38-39 0.04046869200092118 1103 40-41 0.016945638024900234 1103 42-43 -0.017118849752264964 1103 44-45 0.05124538758028052 1103 46-47 0.002978020181380714 1103 48-49 -0.1322590549523568 1103 50-51 -0.026468997232115044 1103 52-53 0.06316554782419814 1103 54-55 0.10123859405910451 1103 56-57 0.17591016377864577 1103 58-59 0.08162748833021283 1103 60-61 0.14206717610214525 1103 62-63 0.11452934219013144 1103 64-65 0.03527980203253378 1103 66-67 -0.09133980928756813 1103 68-69 -0.10131241071605501 1103 70-71 -0.1551317115308919 1103 72-73 -0.18749541378429058 1103 74-75 -0.20053547722895715 1103 76-77 -0.12193197763986774 1103 78-79 -0.06512945767620693 1103 80-81 -0.10726689517873567 1103 82-83 -0.19877123560883447 1103 84-85 -0.1416696261019048 1103 86-87 -0.18966388218382235 1103 88-89 -0.06041646915149812 1103 90-91 -0.1045774900351617 1103 92-93 -0.14510894680444864 1103 94-95 -0.19162596637578133 1103 96-97 -0.21470215041351892 1103 98-99 -0.23369257940576205 1103 100-101 -0.06549622431014512 1104 1 0.2757196291794912 1104 2 0.25906586763188244 1104 3 0.22199281560123296 1104 4 0.2125187354908178 1104 5 0.2388798753681627 1104 6 0.2209215780829581 1104 7 0.2414016550373148 1104 8 0.2830531768406388 1104 9 0.4073881883015389 1104 10-11 0.359502085232279 1104 12-13 0.2642783949014884 1104 14-15 0.4085043474552137 1104 16-17 0.3421409557918551 1104 18-19 0.42920640020179235 1104 20-21 0.33630927732040306 1104 22-23 0.35568060726190254 1104 24-25 0.31923437428768864 1104 26-27 0.4409372697927054 1104 28-29 0.3373327175205105 1104 30-31 0.32906550436752724 1104 32-33 0.5044492700521417 1104 34-35 0.35077136723423763 1104 36-37 0.46136433465015614 1104 38-39 0.5876775216473646 1104 40-41 0.44901573634216874 1104 42-43 0.4147806730473178 1104 44-45 0.4086745679135362 1104 46-47 0.4185169360609322 1104 48-49 0.5839346242999071 1104 50-51 0.5509951510822759 1104 52-53 0.5610025269523362 1104 54-55 0.6244060375636664 1104 56-57 0.5382017739293232 1104 58-59 0.5159785204148548 1104 60-61 0.5706736152251821 1104 62-63 0.5602048931696046 1104 64-65 0.43794771616111916 1104 66-67 0.49654845552783655 1104 68-69 0.46995989768448254 1104 70-71 0.3740929610874275 1104 72-73 0.3570666695387672 1104 74-75 0.2962508183640331 1104 76-77 0.2658884166156419 1104 78-79 0.3063544773982301 1104 80-81 0.34443300288698 1104 82-83 0.3023737268645519 1104 84-85 0.33529343094019026 1104 86-87 0.22092500410190752 1104 88-89 0.3576250772902583 1104 90-91 0.36775767852633123 1104 92-93 0.2596783167532237 1104 94-95 0.16712440354702807 1104 96-97 0.05172730325159236 1104 98-99 0.053704561501945136 1104 100-101 0.1315909418345207 1105 1 0.1850296571484833 1105 2 0.2656160859724963 1105 3 0.3107998727544157 1105 4 0.2618601322918295 1105 5 0.28563214156267946 1105 6 0.25582860241514993 1105 7 0.22377815068644225 1105 8 0.33405671944833415 1105 9 0.3143215145702669 1105 10-11 0.39040987784083114 1105 12-13 0.4093499216275447 1105 14-15 0.4564594371389674 1105 16-17 0.3800819604088659 1105 18-19 0.4594349599681493 1105 20-21 0.4499421844713112 1105 22-23 0.4150186729029244 1105 24-25 0.3812563785863077 1105 26-27 0.42049699739310853 1105 28-29 0.4066638951665169 1105 30-31 0.266568478617522 1105 32-33 0.40194704886999233 1105 34-35 0.3937990138997165 1105 36-37 0.3687770977516962 1105 38-39 0.49009280887528206 1105 40-41 0.27555907159373305 1105 42-43 0.44031175757783103 1105 44-45 0.4140317747236253 1105 46-47 0.374159278391339 1105 48-49 0.5261199878992073 1105 50-51 0.48686627394810955 1105 52-53 0.6113025521200086 1105 54-55 0.5971262166907891 1105 56-57 0.40509458171452906 1105 58-59 0.3533698345746359 1105 60-61 0.43056154303271654 1105 62-63 0.4576055335961513 1105 64-65 0.5863653661086943 1105 66-67 0.38619041224827555 1105 68-69 0.3106878910555295 1105 70-71 0.41379091956223135 1105 72-73 0.6029157204034661 1105 74-75 0.48718049654650386 1105 76-77 0.4792864990645498 1105 78-79 0.3438446225121936 1105 80-81 0.22839555260295086 1105 82-83 0.3172947724857451 1105 84-85 0.3430835129142018 1105 86-87 0.2293544578358251 1105 88-89 0.24578996627530358 1105 90-91 0.35633680387851996 1105 92-93 0.49961382274857513 1105 94-95 0.42007056680133914 1105 96-97 0.24234606499221556 1105 98-99 0.1951455408531686 1105 100-101 0.1316397563291396 1106 1 0.1874736857060455 1106 2 0.2428753914414088 1106 3 0.22620293645442757 1106 4 0.19796049965857065 1106 5 0.2956009500467687 1106 6 0.2709966900286531 1106 7 0.26599552089508194 1106 8 0.22109352543710514 1106 9 0.37858227564325375 1106 10-11 0.28424400829067764 1106 12-13 0.2924687266459145 1106 14-15 0.45806787964260565 1106 16-17 0.455273268373098 1106 18-19 0.4207883251861517 1106 20-21 0.5333177996373166 1106 22-23 0.5248290978006835 1106 24-25 0.47683584763739617 1106 26-27 0.642534843099078 1106 28-29 0.502859223370578 1106 30-31 0.46362157350903743 1106 32-33 0.5554110399977432 1106 34-35 0.5204986049442866 1106 36-37 0.4483820052168639 1106 38-39 0.47623233892311134 1106 40-41 0.5585063232746066 1106 42-43 0.5283210457181156 1106 44-45 0.4419277336894538 1106 46-47 0.4008612954427022 1106 48-49 0.4020411773356827 1106 50-51 0.3985585869722996 1106 52-53 0.3965104955593972 1106 54-55 0.3718541573575962 1106 56-57 0.5043335087263827 1106 58-59 0.39459496800500915 1106 60-61 0.5680398822577786 1106 62-63 0.6389152306918788 1106 64-65 0.319183692857159 1106 66-67 0.4808161140573759 1106 68-69 0.4420644766242461 1106 70-71 0.35812757999059386 1106 72-73 0.3497418103736649 1106 74-75 0.20641283868277682 1106 76-77 0.254656291814257 1106 78-79 0.37677385242832173 1106 80-81 0.35468722793392615 1106 82-83 0.2529404368650354 1106 84-85 0.32828972349159 1106 86-87 0.36219877751420526 1106 88-89 0.5101061789321264 1106 90-91 0.3009378990472982 1106 92-93 0.25800226109334545 1106 94-95 0.1925305376892581 1106 96-97 0.29432949883154436 1106 98-99 0.37131903499609464 1106 100-101 0.1895357152949657 1107 1 0.07466202171460878 1107 2 0.07882242193900169 1107 3 0.16544049664704374 1107 4 0.1263901411780992 1107 5 0.15131791098845326 1107 6 0.1444530838221212 1107 7 0.1546846594718616 1107 8 0.04892391602030699 1107 9 0.13795627243298014 1107 10-11 0.24222822444905745 1107 12-13 0.22666615842761217 1107 14-15 0.3293506511781885 1107 16-17 0.25515555836775405 1107 18-19 0.16431293727816865 1107 20-21 0.1277626899529949 1107 22-23 0.21464584472417414 1107 24-25 0.2214653286432835 1107 26-27 0.29135506781818066 1107 28-29 0.1965751142213037 1107 30-31 0.3837820871527029 1107 32-33 0.18777460340062646 1107 34-35 0.2578233775499541 1107 36-37 0.3045577676566751 1107 38-39 0.4347890537438701 1107 40-41 0.22212456832544092 1107 42-43 0.17997567386150592 1107 44-45 0.3253159091843756 1107 46-47 0.39390704183114167 1107 48-49 0.25369714309277214 1107 50-51 0.201136168566741 1107 52-53 0.17932751000562064 1107 54-55 0.1765959979140419 1107 56-57 0.24950338566592478 1107 58-59 0.26288148485894425 1107 60-61 0.15960622789715728 1107 62-63 0.16845107873895415 1107 64-65 0.10813366610488373 1107 66-67 0.145674327005473 1107 68-69 0.1134382058912351 1107 70-71 0.2125403675775388 1107 72-73 0.11358878736670874 1107 74-75 0.20101289218732532 1107 76-77 0.2478839536655144 1107 78-79 0.32712698244437277 1107 80-81 0.1367725676878031 1107 82-83 0.0024682592299356543 1107 84-85 0.09127661487735494 1107 86-87 0.12280471651902047 1107 88-89 0.28086995635320733 1107 90-91 0.23661473157806512 1107 92-93 0.3111376275994999 1107 94-95 -0.15580279564407462 1107 96-97 0.03738460851586822 1107 98-99 0.1720293078692876 1107 100-101 0.28496508929175945 1108 1 0.02766521376688047 1108 2 0.17784175350394804 1108 3 0.15390701026066012 1108 4 0.1175695545092239 1108 5 0.27191677412145054 1108 6 0.3134534647311682 1108 7 0.2993150484299818 1108 8 0.282515741815601 1108 9 0.25487552833319427 1108 10-11 0.2620724438526665 1108 12-13 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-1.0886994971955986 2316 92-93 -1.0531423435374982 2316 94-95 -0.8841055479376543 2316 96-97 -0.8224791512339849 2316 98-99 -0.9372720528151746 2316 100-101 -0.8458749443817482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7023.0 3 1671.0 4 1781.0 5 1940.0 6 2244.0 7 2728.0 8 3252.0 9 4113.0 10 5156.0 11 6432.0 12 8717.0 13 13078.0 14 12298.0 15 13026.0 16 15722.0 17 21126.0 18 19880.0 19 13413.0 20 12686.0 21 12729.0 22 13628.0 23 14917.0 24 16712.0 25 18684.0 26 21189.0 27 24810.0 28 28850.0 29 34337.0 30 41762.0 31 51377.0 32 64344.0 33 85033.0 34 118991.0 35 179131.0 36 291297.0 37 460246.0 38 454515.0 39 92264.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.166293468435676 18.563704251162868 14.70698672879616 22.563015551605304 2 15.112834037917795 20.5038190122658 40.21207900850071 24.171267941315698 3 20.42746781586508 23.83206863024614 29.907349533229528 25.833114020659252 4 12.710100160842227 16.404536481941804 37.80861602573476 33.07674733148121 5 13.460073369556525 37.594105573737224 34.356640758356214 14.58918029835003 6 31.554052202194427 36.164819704181475 17.159895485981053 15.121232607643046 7 27.95329529138364 33.146930520829955 20.742928121546633 18.15684606623977 8 25.892264044918157 33.95057723848378 19.864267864240453 20.29289085235761 9 25.578174363172252 17.866716123102673 21.08342484601203 35.47168466771305 10-11 25.112108852362564 26.08521871561192 27.062211334265502 21.740461097760015 12-13 26.77867569450995 23.758342262124334 27.303165554288917 22.1598164890768 14-15 22.79920028852837 25.350273487527293 26.907646024660586 24.94288019928375 16-17 21.638905535721463 28.374477908638124 27.604450246474627 22.38216630916579 18-19 21.485458218475294 27.455487465588273 29.584034968619427 21.475019347317005 20-21 22.5232344013745 25.824400627488174 29.605868983630433 22.04649598750689 22-23 21.90054426658119 25.89592201016065 29.888672433216367 22.314861290041794 24-25 22.485107997011315 25.974509369337667 29.76714322599514 21.773239407655872 26-27 21.809513320742006 25.776260705330532 30.5046340069068 21.90959196702066 28-29 21.33087398995334 27.51768538408848 29.636047338932887 21.515393287025294 30-31 21.720260753428818 26.217969542822495 29.386392370216136 22.67537733353255 32-33 21.528585419075313 26.308618767188914 29.76100975474585 22.40178605898993 34-35 22.797906820690304 26.296452231861483 29.27683113696945 21.628809810478764 36-37 22.455529127541524 25.752393941638292 29.964644346523468 21.827432584296716 38-39 21.794409491508326 26.601279395903227 29.688518333002843 21.9157927795856 40-41 22.183519687641667 26.66356343540993 29.3581261800415 21.794790696906897 42-43 21.666915036785525 26.824293807330513 28.640827003716378 22.867964152167584 44-45 22.156253037970735 26.346107637252082 29.02073707360648 22.476902251170706 46-47 23.04476123892113 27.098332835372226 28.360119666590005 21.496786259116643 48-49 22.48014852886071 27.011347432506373 28.384633977536293 22.12387006109662 50-51 21.74003067537415 27.43084225662342 29.066395973725296 21.762731094277132 52-53 22.573325851832813 27.54308149615029 27.87027866907866 22.013313982938236 54-55 21.82548107456226 27.080972764519363 28.420300853587797 22.673245307330582 56-57 22.471691448420888 26.99063002358024 28.08338515698728 22.454293371011595 58-59 23.056376516919645 26.708213321208362 27.914300800651226 22.32110936122076 60-61 22.029992561536847 27.1445258798726 29.066545036607 21.758936521983557 62-63 21.716077118818916 27.293115701153113 28.92478393434355 22.06602324568442 64-65 21.480494219664443 28.004970723958568 28.251053205963313 22.26348185041368 66-67 21.815771700105838 27.24639110357868 27.895940858704638 23.04189633761084 68-69 21.91646342680188 27.01854286134358 28.412325269720494 22.65266844213404 70-71 22.533883953308305 26.972305705090804 27.819954974294614 22.673855367306274 72-73 22.18546547080965 26.780627630002897 28.701462837166115 22.33244406202134 74-75 21.744372511010955 28.255856836056292 27.782622097498187 22.217148555434562 76-77 22.038025969737895 27.126475787793808 27.892039631021525 22.943458611446772 78-79 22.178329493483716 27.401694118725857 28.011485162133354 22.408491225657073 80-81 22.77003807825184 27.145483680912964 27.646553127299654 22.437925113535545 82-83 22.37755054781436 27.076973729764482 28.36104618717262 22.18442953524854 84-85 22.11299062221353 27.721182526011255 27.972267192971557 22.193559658803657 86-87 22.219356146379894 27.76367668463352 27.696954439024125 22.32001272996246 88-89 21.900630296325954 27.833180379957284 27.028122782806662 23.2380665409101 90-91 22.228358617678047 27.149069915612838 27.478829817927945 23.143741648781162 92-93 22.79027698020574 27.247494298761417 27.71765154837017 22.244577172662673 94-95 22.30111632867193 26.940754883579594 28.002224630327216 22.755904157421263 96-97 21.795300489601136 26.735437416461867 28.70803828374482 22.76122381019218 98-99 22.026004273808088 27.37697895248129 27.397756319553153 23.199260454157468 100-101 22.1558215353769 26.177200711936027 28.213262537187013 23.45371521550006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2304.0 1 1343.5 2 449.5 3 509.0 4 458.5 5 456.0 6 745.5 7 1532.5 8 2547.0 9 2999.0 10 2779.5 11 2538.0 12 2588.5 13 2772.0 14 2954.5 15 3300.5 16 3673.5 17 3865.0 18 4117.5 19 4527.5 20 5069.0 21 5786.5 22 6899.0 23 8294.0 24 9969.5 25 12321.5 26 15419.0 27 19053.0 28 22224.0 29 25016.5 30 28102.0 31 32649.0 32 38962.0 33 41633.0 34 42101.5 35 45297.5 36 49665.0 37 53165.0 38 55683.0 39 57921.0 40 59985.5 41 62698.0 42 64079.0 43 64878.0 44 66516.5 45 69948.0 46 73130.0 47 85942.0 48 110202.5 49 106569.0 50 82825.0 51 72754.0 52 70077.5 53 69461.5 54 69787.0 55 65660.5 56 59198.0 57 53718.0 58 50091.5 59 44886.5 60 36288.5 61 30402.5 62 25860.0 63 19560.5 64 13421.0 65 9825.0 66 7380.5 67 5770.5 68 4716.5 69 3827.5 70 2980.0 71 2073.0 72 1541.5 73 1232.5 74 931.0 75 690.5 76 501.0 77 322.5 78 223.5 79 164.0 80 123.0 81 97.0 82 70.0 83 45.5 84 32.0 85 28.0 86 23.0 87 17.5 88 18.0 89 16.0 90 10.0 91 7.5 92 6.5 93 5.5 94 6.5 95 4.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3353453445504556 2 0.12093973367701516 3 0.11491556581083931 4 0.1229477896324071 5 0.14024445342998776 6 0.09100144488753517 7 0.09980950851004985 8 0.10514911184597846 9 0.0931920513843264 10-11 0.09159473414708279 12-13 0.09558802724019179 14-15 0.09741353265418447 16-17 0.08680278243535201 18-19 0.10222830318359016 20-21 0.1116981125186772 22-23 0.12014107505839336 24-25 0.13321625758611594 26-27 0.13212095433772034 28-29 0.12710081444924046 30-31 0.11833838846207559 32-33 0.11156119961262774 34-35 0.3296634589494034 36-37 0.48455759332668247 38-39 0.4594112562489332 40-41 0.4370716337451978 42-43 0.46956563011426755 44-45 0.47654818832278956 46-47 0.4844663180559827 48-49 0.49548780699296363 50-51 0.4836448406196861 52-53 0.48761531489512017 54-55 0.473673017295751 56-57 0.45174413351016396 58-59 0.43127565405577095 60-61 0.39246084519075164 62-63 0.4620354202815477 64-65 0.49322874404314765 66-67 0.5007133162405176 68-69 0.5147240702929115 70-71 0.5059388254880717 72-73 0.49856834737907624 74-75 0.5052998985931743 76-77 0.48188779165871815 78-79 0.46089447939780226 80-81 0.46271998481179494 82-83 0.47540724743904406 84-85 0.4762515436930157 86-87 0.47883007009028034 88-89 0.49156297035287927 90-91 0.5067146652890187 92-93 0.4995495565390973 94-95 0.5036797625382557 96-97 0.5034743931791816 98-99 0.4982260651139526 100-101 0.48563007775740313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2191174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.27956521863143 #Duplication Level Percentage of deduplicated Percentage of total 1 75.24304787369542 39.336738285614665 2 13.846590560954297 14.477874685741929 3 4.712650406574078 7.391259428492983 4 2.149401313709491 4.494790646443496 5 1.0960120276362273 2.8649516139606304 6 0.684890986507952 2.1483481798077175 7 0.4653219018738054 1.7028778701668454 8 0.2997930217062879 1.2538439064307574 9 0.20792806819608117 0.9783350101836961 >10 1.1691117341889974 11.12404664069563 >50 0.07852189376950128 2.782598788637432 >100 0.03861050882045566 3.7031070064466842 >500 0.004103505497509946 1.495726944185232 >1k 0.0034923451042637578 3.709077832234468 >5k 4.365431380329697E-4 1.7914124745472086 >10k+ 8.730862760659394E-5 0.7450106864106693 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16322 0.7448974841797137 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9673 0.4414528467387802 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8698 0.39695615227270864 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7303 0.33329165095971386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7226 0.3297775530377779 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6347 0.2896620715652888 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4824 0.2201559529275174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4681 0.21362977107249356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4060 0.18528879952025717 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3963 0.18086194889132493 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3623 0.16534515287238713 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3456 0.15772366776896768 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3238 0.14777466326270758 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3167 0.14453439115287056 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2674 0.12203503692541076 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2330 0.1063356903650737 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2230 0.101771926830092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.281881767490852E-5 0.0 12-13 0.0 0.0 0.0 4.563763534981704E-5 0.0 14-15 0.0 0.0 0.0 1.3691290604945112E-4 0.0 16-17 2.281881767490852E-5 0.0 0.0 1.3691290604945112E-4 0.0 18-19 4.563763534981704E-5 0.0 0.0 2.281881767490852E-4 0.0 20-21 4.563763534981704E-5 0.0 0.0 3.4228226512362776E-4 0.0 22-23 4.563763534981704E-5 0.0 0.0 6.1610807722253E-4 0.0 24-25 4.563763534981704E-5 0.0 0.0 7.986586186217982E-4 0.0 26-27 4.563763534981704E-5 0.0 0.0 0.0010040279776959747 0.0 28-29 4.563763534981704E-5 0.0 0.0 0.002806714574013748 0.0 30-31 4.563763534981704E-5 0.0 0.0 0.007940948550868163 0.0 32-33 4.563763534981704E-5 0.0 0.0 0.01823223532225191 0.0 34-35 4.563763534981704E-5 0.0 0.0 0.03258527163976937 0.0 36-37 4.563763534981704E-5 0.0 0.0 0.058096709800317094 0.0 38-39 6.845645302472556E-5 0.0 0.0 0.0950860132513438 0.0 40-41 9.127527069963408E-5 0.0 0.0 0.1363196167899035 0.0 42-43 9.127527069963408E-5 0.0 0.0 0.17908208111268206 0.0 44-45 9.127527069963408E-5 0.0 0.0 0.23615194411762827 0.0 46-47 9.127527069963408E-5 0.0 0.0 0.301573494391591 0.0 48-49 9.127527069963408E-5 0.0 0.0 0.3748903555810721 0.0 50-51 9.127527069963408E-5 0.0 0.0 0.4579964895530889 0.0 52-53 9.127527069963408E-5 0.0 0.0 0.5402126896357843 0.0 54-55 9.127527069963408E-5 0.0 0.0 0.6311684968879696 0.0 56-57 9.127527069963408E-5 0.0 0.0 0.7477498363890773 0.0 58-59 9.127527069963408E-5 0.0 0.0 0.8738238040429469 0.0 60-61 9.127527069963408E-5 0.0 0.0 1.0123568461473165 0.0 62-63 9.127527069963408E-5 0.0 0.0 1.157347613653685 0.0 64-65 9.127527069963408E-5 0.0 0.0 1.311831009312816 0.0 66-67 9.127527069963408E-5 0.0 0.0 1.500337262125235 0.0 68-69 1.3691290604945112E-4 0.0 0.0 1.7190556295392332 0.0 70-71 1.3691290604945112E-4 0.0 0.0 1.9490693117023112 0.0 72-73 1.3691290604945112E-4 0.0 0.0 2.1861340085269356 0.0 74-75 1.3691290604945112E-4 0.0 0.0 2.4397195293481944 0.0 76-77 1.3691290604945112E-4 0.0 0.0 2.7051708353604047 0.0 78-79 1.3691290604945112E-4 0.0 0.0 3.032438318453943 0.0 80-81 1.5973172372435964E-4 0.0 0.0 3.3892105328011377 0.0 82-83 1.8255054139926816E-4 0.0 0.0 3.7451840885297107 0.0 84-85 1.8255054139926816E-4 0.0 0.0 4.112726784819462 0.0 86-87 1.8255054139926816E-4 0.0 0.0 4.501741988541303 0.0 88-89 1.8255054139926816E-4 0.0 0.0 4.9347518727403665 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 7505 0.0 23.549889 2 GTACATG 7710 0.0 23.335669 1 ACATGGG 7595 0.0 21.711119 3 GAGTACT 9135 0.0 21.258974 12-13 TAAGGTG 1230 0.0 21.18792 5 GTACTTT 9460 0.0 20.304928 14-15 GTAAGGT 1105 0.0 20.154236 4 GTATAGG 495 0.0 20.13954 1 AGTACTT 9395 0.0 19.68753 12-13 AGAGTAC 12730 0.0 19.64503 10-11 AGGTAAG 1070 0.0 19.042126 2 GTGTAGC 1230 0.0 18.525568 1 GGTAAGG 1145 0.0 18.208656 3 CATGGGG 4350 0.0 18.082129 4 ACTTTTT 11190 0.0 17.421125 16-17 TACTTTT 10715 0.0 17.06463 14-15 ACGTGCG 170 0.0018177711 16.723728 5 CCTACAC 910 0.0 16.662468 3 GTATAGC 1045 0.0 16.35391 1 GCACCGT 325 1.9760228E-7 16.03213 6 >>END_MODULE