FastQCFastQC Report
Fri 27 May 2016
SRR939219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191174
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT180260.8226640148158019No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88710.4048514631882269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.39777762970900526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.3580728869546645No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65620.2994741631654994No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC60290.27514930352404693No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50820.23193046284777022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49400.22544991862809616No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC44510.20313311494203562No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41440.18912236088964182No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39610.1807706736206253No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38430.1753854326493469No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA36440.16630354321473328No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30260.13809948456854637No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27960.12760282843808843No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22830.1041907215036323No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22020.10049407304029712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG12700.023.536135
GTAAGGT11900.021.9287554
GAGTACT100850.021.64262412-13
GTACCGT1805.2236246E-621.0870466
GTACTTT103400.020.90238414-15
GTACATG77700.020.521621
TACATGG74050.020.3751262
GTAAGCG2450.020.33486494-95
AGTACTT103500.020.0111412-13
AGAGTAC140800.019.79854210-11
GGTAAGG13150.019.1226623
AGGTAAG12000.018.978342
ACATGGG77550.018.7213783
ACTTTTT120900.017.89561816-17
TACTTTT115250.017.7445214-15
GGTGTGC18050.017.6114798
TAGACTC10900.017.411325
TCTAGAC11800.016.4854223
CATGGGG46700.016.2555374
GCTATAC7350.015.4925223
Produced by FastQC (version 0.11.5)