##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.451060481732625 34.0 31.0 34.0 31.0 34.0 2 32.66533602534532 34.0 31.0 34.0 31.0 34.0 3 32.75130774644095 34.0 31.0 34.0 31.0 34.0 4 36.21807670226098 37.0 35.0 37.0 35.0 37.0 5 36.184622946420504 37.0 35.0 37.0 35.0 37.0 6 36.23005019227136 37.0 37.0 37.0 35.0 37.0 7 36.18612122998904 37.0 37.0 37.0 35.0 37.0 8 36.17456760622388 37.0 37.0 37.0 35.0 37.0 9 37.9100463952201 39.0 38.0 39.0 35.0 39.0 10-11 37.842941957142614 39.0 38.0 39.0 35.0 39.0 12-13 37.781806921768876 39.0 38.0 39.0 35.0 39.0 14-15 39.10512287020565 40.0 38.0 41.0 36.0 41.0 16-17 39.058784697153214 40.0 38.0 41.0 36.0 41.0 18-19 39.10974641904294 40.5 38.5 41.0 36.0 41.0 20-21 39.18134821789597 41.0 39.0 41.0 36.0 41.0 22-23 39.14183903240911 40.5 39.0 41.0 36.0 41.0 24-25 39.07385606985114 40.5 39.0 41.0 35.0 41.0 26-27 38.91802750489008 40.0 38.0 41.0 35.0 41.0 28-29 38.77178968899777 40.0 38.0 41.0 35.0 41.0 30-31 38.566365792949355 40.0 38.0 41.0 34.0 41.0 32-33 38.38275006914101 40.0 38.0 41.0 34.0 41.0 34-35 38.29113320074079 40.0 38.0 41.0 34.0 41.0 36-37 38.15351747510695 40.0 38.0 41.0 33.5 41.0 38-39 37.99398473147272 40.0 38.0 41.0 33.0 41.0 40-41 37.828777404259085 40.0 37.5 41.0 33.0 41.0 42-43 37.63385929186819 40.0 37.0 41.0 33.0 41.0 44-45 37.383863399255375 40.0 37.0 41.0 32.5 41.0 46-47 37.051500702363214 40.0 37.0 41.0 31.5 41.0 48-49 36.67393484040975 40.0 36.0 41.0 30.5 41.0 50-51 36.380928442926034 39.5 36.0 41.0 30.0 41.0 52-53 36.14942948392049 39.0 35.0 41.0 30.0 41.0 54-55 35.89933273213355 39.0 35.0 41.0 29.0 41.0 56-57 35.58705264848889 39.0 35.0 40.0 28.0 41.0 58-59 35.26349139776211 38.0 35.0 40.0 28.0 41.0 60-61 34.858824310620705 38.0 34.0 40.0 27.0 41.0 62-63 34.479324782057475 37.5 34.0 40.0 26.0 41.0 64-65 34.06204322431719 37.0 34.0 39.5 26.0 41.0 66-67 33.67523300294728 36.0 33.0 39.0 25.5 40.5 68-69 33.19008394586646 36.0 33.0 39.0 23.5 40.0 70-71 32.71956699924333 35.0 32.5 38.5 22.5 40.0 72-73 32.2382651948225 35.0 32.0 37.5 21.5 40.0 74-75 31.757046222709835 35.0 31.5 37.0 20.0 39.0 76-77 29.803971067564696 33.0 29.0 35.0 17.0 37.5 78-79 30.78001838283952 34.0 31.0 36.0 17.5 38.0 80-81 30.80760952804296 34.0 31.0 36.0 17.5 37.0 82-83 30.499625771390132 34.0 31.0 35.0 12.5 37.0 84-85 30.10800990701788 34.0 30.5 35.0 7.5 36.5 86-87 29.576256381282363 34.0 30.0 35.0 2.0 36.0 88-89 29.00190149207685 34.0 29.0 35.0 2.0 36.0 90-91 28.517545160722058 34.0 29.0 35.0 2.0 35.0 92-93 28.22482856222281 34.0 29.0 35.0 2.0 35.0 94-95 27.865733848612663 34.0 28.5 35.0 2.0 35.0 96-97 27.267355079970827 33.0 27.0 35.0 2.0 35.0 98-99 26.46841099793992 33.0 25.5 35.0 2.0 35.0 100-101 22.83494624342932 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2427713642942919 1101 2 0.24240053429107178 1101 3 0.21365132114784302 1101 4 0.19934999895212968 1101 5 0.17511056907355282 1101 6 0.1243878826710727 1101 7 0.17715928019448057 1101 8 0.24859383183942185 1101 9 0.34343272479394216 1101 10-11 0.2723730816747647 1101 12-13 0.2455148082610279 1101 14-15 0.3115023131576109 1101 16-17 0.3329221658308299 1101 18-19 0.3524209046734086 1101 20-21 0.32358527569075335 1101 22-23 0.33331849666752333 1101 24-25 0.28674977920374545 1101 26-27 0.3585495928357787 1101 28-29 0.3156530759431533 1101 30-31 0.36333905212247686 1101 32-33 0.2765673390508141 1101 34-35 0.308280491681316 1101 36-37 0.33664568045207943 1101 38-39 0.31524129531157286 1101 40-41 0.31740356742514564 1101 42-43 0.3043488289845655 1101 44-45 0.4229256881412553 1101 46-47 0.399525132326346 1101 48-49 0.3344062475748899 1101 50-51 0.3461260409777154 1101 52-53 0.3287077434509058 1101 54-55 0.37241274777147026 1101 56-57 0.24568903813257492 1101 58-59 0.30968828165705276 1101 60-61 0.3247241888303236 1101 62-63 0.23166209761352263 1101 64-65 0.00997592038127948 1101 66-67 0.004613016156504557 1101 68-69 0.18077921975666555 1101 70-71 0.24354220417237826 1101 72-73 0.14071390708933507 1101 74-75 0.10675385102621604 1101 76-77 0.33714296191135773 1101 78-79 0.3865308158973484 1101 80-81 0.15202399041137582 1101 82-83 0.24132693301294594 1101 84-85 0.31332222029385903 1101 86-87 0.36369845680914636 1101 88-89 0.5592405444431314 1101 90-91 0.6952259336301196 1101 92-93 0.7162197763124425 1101 94-95 0.570079016645618 1101 96-97 0.27132625708123825 1101 98-99 0.31951088465422117 1101 100-101 0.11688204439393601 1102 1 0.13244581604755723 1102 2 0.10532422706145894 1102 3 0.12898977894614205 1102 4 0.14073618049943803 1102 5 0.05222618187609385 1102 6 0.09326649853959879 1102 7 0.09698965013591021 1102 8 0.047281839292665495 1102 9 0.19807723851627657 1102 10-11 0.15752945871417268 1102 12-13 0.18397064771998117 1102 14-15 0.2286094949040276 1102 16-17 0.16881670303239105 1102 18-19 0.1646647462725923 1102 20-21 0.21872985139825118 1102 22-23 0.17091266179708242 1102 24-25 0.18229497766093772 1102 26-27 0.2313829965620826 1102 28-29 0.08669797950120284 1102 30-31 0.15198103491797355 1102 32-33 0.22774717707364545 1102 34-35 0.24293215918965672 1102 36-37 0.40438849191681214 1102 38-39 0.36680235553163953 1102 40-41 0.3743483480325338 1102 42-43 0.30399610386437104 1102 44-45 0.38555144960623267 1102 46-47 0.3865411180595615 1102 48-49 0.5008111990896111 1102 50-51 0.4232035235105158 1102 52-53 0.37035734854367774 1102 54-55 0.2753097627697372 1102 56-57 0.30780622605604435 1102 58-59 0.2549335094699856 1102 60-61 0.25752207049392695 1102 62-63 0.1546683353070506 1102 64-65 0.09652089934120767 1102 66-67 0.3118783217970531 1102 68-69 0.2555766677924325 1102 70-71 0.11256445926254344 1102 72-73 0.21354245396916838 1102 74-75 0.2278150424616392 1102 76-77 0.28114633970048786 1102 78-79 0.19020701933535378 1102 80-81 0.09661488562209541 1102 82-83 0.24477167149188972 1102 84-85 0.34488165977620966 1102 86-87 0.5166168043650785 1102 88-89 0.5424334406745963 1102 90-91 0.4746734499032357 1102 92-93 0.4488910311337371 1102 94-95 0.3771827851828071 1102 96-97 0.41889388933907057 1102 98-99 0.11165060190526077 1102 100-101 0.28018062667776533 1103 1 0.055970407240948816 1103 2 0.03702966023319476 1103 3 0.05879927464665968 1103 4 0.08251159014334064 1103 5 0.08597941590689118 1103 6 0.062916323127439 1103 7 0.0732404560270794 1103 8 0.11130047317107739 1103 9 0.0713901001206878 1103 10-11 0.12758275219251658 1103 12-13 0.10924936047106115 1103 14-15 0.2542070056422645 1103 16-17 0.16367182054597862 1103 18-19 0.15440480973913395 1103 20-21 0.13057687022888587 1103 22-23 0.11570152877690276 1103 24-25 0.11126352069342715 1103 26-27 0.153355200643027 1103 28-29 0.2033031053876826 1103 30-31 0.22752679069736814 1103 32-33 0.16443343631522822 1103 34-35 0.34476828279034066 1103 36-37 0.454813991987983 1103 38-39 0.34762790207238226 1103 40-41 0.4887262837789379 1103 42-43 0.38322907339540535 1103 44-45 0.5059285648088974 1103 46-47 0.49361684252432525 1103 48-49 0.6279897217759753 1103 50-51 0.5974856000722681 1103 52-53 0.6136757781234365 1103 54-55 0.5756620000073482 1103 56-57 0.6269069457063878 1103 58-59 0.6217275488058434 1103 60-61 0.5802623830541123 1103 62-63 0.5331362838639819 1103 64-65 0.49340529268307876 1103 66-67 0.424510770533999 1103 68-69 0.4103894079898609 1103 70-71 0.37126886884782806 1103 72-73 0.40833760062042757 1103 74-75 0.23228276704362472 1103 76-77 0.2019908194506428 1103 78-79 0.35524149473160094 1103 80-81 0.4230314225088847 1103 82-83 0.3406949611187109 1103 84-85 0.20470683372311171 1103 86-87 0.41679593199312137 1103 88-89 0.5490485512386805 1103 90-91 0.5237909467424693 1103 92-93 0.2897667731815794 1103 94-95 0.3060254731333707 1103 96-97 0.14983325492479693 1103 98-99 0.026660743574645096 1103 100-101 -0.26992277857873503 1104 1 0.1448489862441278 1104 2 0.092558156620278 1104 3 0.15222893176931507 1104 4 -0.006520095800418346 1104 5 0.14014228058912437 1104 6 0.14125107449820717 1104 7 0.12140080934446473 1104 8 0.11536911029324415 1104 9 0.06026261650004727 1104 10-11 0.11324105038318777 1104 12-13 0.09454749770420534 1104 14-15 0.1514439905247542 1104 16-17 0.125600854965235 1104 18-19 0.20073661405819365 1104 20-21 0.15062565893720858 1104 22-23 0.25914089637330306 1104 24-25 0.2413982548011404 1104 26-27 0.20619995842579186 1104 28-29 0.22199859315146142 1104 30-31 0.15210286943338502 1104 32-33 0.12411390521294408 1104 34-35 0.13186203561613752 1104 36-37 0.013269759495607047 1104 38-39 0.037847862285126155 1104 40-41 0.2122274798626833 1104 42-43 0.1984796036180967 1104 44-45 0.13250792969253666 1104 46-47 0.128804105670973 1104 48-49 -0.10966667336735725 1104 50-51 -0.24822961485288175 1104 52-53 -0.1882367228013635 1104 54-55 0.05953927400506842 1104 56-57 0.011015524216340111 1104 58-59 0.01812912467701011 1104 60-61 0.05573521633400702 1104 62-63 -0.028642366657607 1104 64-65 0.0648545988723015 1104 66-67 0.048593413344747205 1104 68-69 0.07009741558560734 1104 70-71 0.05049107531408481 1104 72-73 -0.08514995969753159 1104 74-75 -0.13178421570153276 1104 76-77 -0.18465070274061546 1104 78-79 -0.003979756100726206 1104 80-81 0.07291104346131405 1104 82-83 0.07060532676685582 1104 84-85 0.04755129351250176 1104 86-87 -0.0017216331749452252 1104 88-89 0.03497966750130743 1104 90-91 0.21928487589813983 1104 92-93 0.20984546398365467 1104 94-95 0.10168396656107603 1104 96-97 0.0901156396116356 1104 98-99 0.251936798471192 1104 100-101 0.030341510717342146 1105 1 0.07715284886885598 1105 2 0.16627221357575905 1105 3 0.1625236388553688 1105 4 0.1223594913516095 1105 5 0.16038386082470169 1105 6 0.07145542325643106 1105 7 0.09727197636411944 1105 8 0.12126855047193885 1105 9 0.07182944403378144 1105 10-11 0.07626235361735922 1105 12-13 0.17574783386191228 1105 14-15 0.18141866585930444 1105 16-17 0.17171129641016591 1105 18-19 0.19343541292727195 1105 20-21 0.23290849944037717 1105 22-23 0.2875474980553676 1105 24-25 0.1549464356885224 1105 26-27 0.2176203424756693 1105 28-29 0.20224098777961075 1105 30-31 0.3254631316874139 1105 32-33 0.26463047540352136 1105 34-35 0.35804068489209584 1105 36-37 0.3365302586593799 1105 38-39 0.4731360321536826 1105 40-41 0.3270299724926744 1105 42-43 0.40019152058537344 1105 44-45 0.4169562449546973 1105 46-47 0.392415377452906 1105 48-49 0.41805063821482946 1105 50-51 0.49881036250111066 1105 52-53 0.42883772886958127 1105 54-55 0.49027432708040664 1105 56-57 0.4984406405739108 1105 58-59 0.5353935113388317 1105 60-61 0.7073914259056764 1105 62-63 0.5943478375734088 1105 64-65 0.4134628215880696 1105 66-67 0.4471210798794516 1105 68-69 0.32173956952640737 1105 70-71 0.4650166499391801 1105 72-73 0.4095258262035628 1105 74-75 0.5599597324049803 1105 76-77 0.6107363520478906 1105 78-79 0.6136992348766839 1105 80-81 0.33054989862159445 1105 82-83 0.39855189476446284 1105 84-85 0.6815680543003815 1105 86-87 0.6322020366546042 1105 88-89 0.815810926831869 1105 90-91 0.908526038634875 1105 92-93 0.6830542786548861 1105 94-95 0.29300084916442515 1105 96-97 0.31602470532183347 1105 98-99 0.3586564448089504 1105 100-101 0.2407427756750593 1106 1 0.05424857216544865 1106 2 0.05818529321809507 1106 3 0.09109021541321027 1106 4 0.024901253325687378 1106 5 0.04558452624370801 1106 6 0.1303872871227938 1106 7 0.13459643744403849 1106 8 0.11845784831625394 1106 9 0.12704050662475197 1106 10-11 0.16936556287776483 1106 12-13 0.13836376232936942 1106 14-15 0.13363499635074305 1106 16-17 0.11037768581891072 1106 18-19 0.12478808500046057 1106 20-21 0.15789394339962115 1106 22-23 0.1784846298222007 1106 24-25 0.2519139271974993 1106 26-27 0.27247686150249706 1106 28-29 0.30293725172914776 1106 30-31 0.19629421230015254 1106 32-33 0.2166111366636585 1106 34-35 0.08976973459056836 1106 36-37 0.23617432880563172 1106 38-39 0.33646936548701234 1106 40-41 0.18345334533526625 1106 42-43 0.149783951444185 1106 44-45 0.2688031292688251 1106 46-47 0.17222892150225988 1106 48-49 0.18788809575971754 1106 50-51 0.19239704025809345 1106 52-53 0.22728785710137345 1106 54-55 0.18420584437436816 1106 56-57 0.2278694892603994 1106 58-59 0.1197211608848221 1106 60-61 0.024264996294526497 1106 62-63 0.011890639264677816 1106 64-65 0.19869710452977074 1106 66-67 0.22258132802528507 1106 68-69 0.06182378661936383 1106 70-71 0.18404035549126974 1106 72-73 0.033164836392785446 1106 74-75 0.005547865766036608 1106 76-77 0.1564405871626029 1106 78-79 0.2355637943831681 1106 80-81 0.1116013992800795 1106 82-83 0.07148909584577012 1106 84-85 0.15386478958838623 1106 86-87 0.26306916079683873 1106 88-89 0.26646144322474186 1106 90-91 0.4474901337343411 1106 92-93 0.29466514716531833 1106 94-95 0.1237040445619435 1106 96-97 0.2617365650293273 1106 98-99 0.47626440211123366 1106 100-101 -0.12074159390974515 1107 1 -0.07339722772753987 1107 2 -0.07831015889533433 1107 3 0.011189198826798474 1107 4 0.012680814588392764 1107 5 0.04892588525922292 1107 6 0.0716687210446807 1107 7 0.029695420857628108 1107 8 0.023942064474631763 1107 9 0.08910043171838566 1107 10-11 0.05043832906289225 1107 12-13 0.03829955687190534 1107 14-15 0.12206463787027388 1107 16-17 0.08094082118541479 1107 18-19 0.05516529206306586 1107 20-21 0.08508495463558319 1107 22-23 0.10767136069431871 1107 24-25 0.07934838412633383 1107 26-27 0.14148836498029027 1107 28-29 0.13057770116734702 1107 30-31 0.10249538940021097 1107 32-33 0.09274142023776477 1107 34-35 0.01220285390571263 1107 36-37 -0.00730747964808387 1107 38-39 0.07777086361435437 1107 40-41 0.01570856202868498 1107 42-43 0.03547683533931689 1107 44-45 0.0064823695042477425 1107 46-47 0.02635412214430488 1107 48-49 0.07564090474847518 1107 50-51 -0.009515747328848079 1107 52-53 0.07525200905428875 1107 54-55 0.08588454956430525 1107 56-57 0.09740399969378899 1107 58-59 0.0485453984450146 1107 60-61 0.136513524919458 1107 62-63 -0.18845981552397006 1107 64-65 -0.02523280451249832 1107 66-67 -0.1597046537831659 1107 68-69 -0.12633298170594998 1107 70-71 0.09468909813440263 1107 72-73 0.11611427459503432 1107 74-75 -0.00933308661491239 1107 76-77 0.03782769256656593 1107 78-79 0.12051965313116142 1107 80-81 -0.05760539579749846 1107 82-83 -0.07797853390275478 1107 84-85 0.19658817107581328 1107 86-87 0.239592702797907 1107 88-89 0.22909787982185392 1107 90-91 0.24211560189913328 1107 92-93 0.28236177637879933 1107 94-95 0.29209757051164686 1107 96-97 0.26165898343489147 1107 98-99 0.2416482972423175 1107 100-101 0.02520944942893877 1108 1 0.06169406117560072 1108 2 0.06745994095109609 1108 3 0.11585365415271554 1108 4 0.10568423932266313 1108 5 0.11738760734729681 1108 6 0.12614496248930607 1108 7 0.05522580654096032 1108 8 0.07102397370796609 1108 9 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94-95 -0.0013483800776405985 2316 96-97 -0.11157710906416085 2316 98-99 -0.05758880889794327 2316 100-101 0.1296930937267753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 15.0 5 35.0 6 93.0 7 182.0 8 559.0 9 1040.0 10 1972.0 11 3051.0 12 4612.0 13 6481.0 14 8208.0 15 11259.0 16 14738.0 17 21123.0 18 21060.0 19 14308.0 20 12588.0 21 12637.0 22 13152.0 23 14283.0 24 15835.0 25 18474.0 26 21681.0 27 26181.0 28 31683.0 29 38145.0 30 46369.0 31 57048.0 32 71540.0 33 92765.0 34 128294.0 35 188989.0 36 308343.0 37 503039.0 38 436930.0 39 44450.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34326463546657 19.17205362719974 15.161553862583506 22.32312787475019 2 15.422371751399023 21.11242648917886 39.57490368177059 23.890298077651522 3 20.45661366920199 24.02689152025353 30.19970116476373 25.316793645780756 4 12.964511261999276 17.021377581150563 37.404697207980746 32.60941394886942 5 13.267134422003913 37.78974193742715 34.37130049918446 14.571823141384483 6 31.39641123890663 36.7066695753053 16.88907407627144 15.007845109516635 7 27.89536568068077 33.29534760817717 20.654315905537395 18.154970805604666 8 25.85139290626851 34.424240156190244 19.479557533997756 20.24480940354349 9 25.389083660174865 17.885571844134695 20.95785181824903 35.76749267744141 10-11 25.133992097387065 26.379146521453794 26.864274585222354 21.62258679593679 12-13 26.81863694987253 23.867205434164514 27.249068307674335 22.065089308288616 14-15 22.639574124440678 25.62628456707679 26.85971996979677 24.87442133868576 16-17 21.44654474109547 28.5711058439524 27.669248801597156 22.313100613354976 18-19 21.32942036069265 27.55777384989684 29.689257762851224 21.423548026559292 20-21 22.39657827265201 25.88765200755394 29.755418784633257 21.960350935160786 22-23 21.79249571234416 25.902666789584032 30.071368134160043 22.233469363911766 24-25 22.413878477748682 26.034148539873243 29.967425966809856 21.58454701556822 26-27 21.736650100780437 25.837785144281572 30.656853856845462 21.76871089809253 28-29 21.222162183377495 27.58612506355041 29.789327547698175 21.40238520537392 30-31 21.65233368977514 26.24000445438161 29.546470016671982 22.561191839171265 32-33 21.492907455090286 26.35842703500498 29.871018914974346 22.27764659493039 34-35 22.687623134118027 26.32037771665272 29.44912822242543 21.54287092680382 36-37 22.38800894107024 25.77441761042982 30.112041844902603 21.725531603597336 38-39 21.699326743954092 26.64483038959747 29.875012381904025 21.780830484544417 40-41 22.07877805285951 26.738469389245523 29.440576868304014 21.742175689590955 42-43 21.629261753604762 26.901162529316547 28.75907338145389 22.7105023356248 44-45 22.128495250821956 26.31855182473224 29.10875097438931 22.4442019500565 46-47 22.973112046034306 27.163357481247846 28.461306935609336 21.402223537108508 48-49 22.501051953255853 27.099342723176107 28.41925882248779 21.98034650108025 50-51 21.701417848206837 27.458404119255786 29.052025798972924 21.78815223356445 52-53 22.62512938281431 27.64278660004783 27.818309105811867 21.91377491132599 54-55 21.811275599290607 27.126439981489376 28.379284347112556 22.683000072107465 56-57 22.48452199597111 27.042398275992685 28.03670543735915 22.43637429067705 58-59 23.08247005806285 26.764431507105645 27.876785539876792 22.27631289495471 60-61 21.9489724683922 27.23125925253126 29.047180121723205 21.772588157353336 62-63 21.7458583153399 27.360471585434926 28.83401633078162 22.059653768443553 64-65 21.443403984983064 28.08720265288258 28.200318010009468 22.26907535212489 66-67 21.801377765526105 27.26648088343487 27.871752064275036 23.060389286763993 68-69 21.861338845355245 27.12844454779232 28.285859614588997 22.724356992263438 70-71 22.521511299422137 27.137826571509155 27.7388285914309 22.601833537637813 72-73 22.165629018964264 26.84795913058479 28.623970529040598 22.362441321410348 74-75 21.649581457246207 28.387864222558317 27.657890245137995 22.30466407505748 76-77 21.927968839078208 27.14321094428359 27.90574612392243 23.02307409271577 78-79 22.064939000458203 27.45533880622354 28.04050723724924 22.439214956069012 80-81 22.617631991200778 27.13665837175577 27.74694474585283 22.49876489119062 82-83 22.22608370233541 27.15964050963931 28.355492473726784 22.258783314298494 84-85 21.843062212311757 27.821752174861516 28.04642625368866 22.28875935913807 86-87 22.018728787115265 27.773054285339654 27.61072960216518 22.597487325379895 88-89 21.71820041387072 27.84795016688631 27.0589747443551 23.37487467488787 90-91 21.94369553409659 27.190680172481134 27.464365574238798 23.401258719183478 92-93 22.43119441906485 27.42157856929664 27.63053048274578 22.51669652889273 94-95 21.89896831561528 27.025877451996056 27.95362212220481 23.121532110183857 96-97 21.321720684893123 26.92442498861341 28.635311481425024 23.118542845068443 98-99 21.554974884095802 27.476637177471197 27.393621087456015 23.574766850976992 100-101 21.483126817392385 26.285458191104276 28.35836085497394 23.873054136529394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 500.0 1 360.0 2 304.5 3 389.5 4 346.5 5 352.0 6 634.5 7 1413.0 8 2328.5 9 2767.0 10 2487.0 11 2093.0 12 2168.5 13 2386.5 14 2596.0 15 2826.0 16 3104.5 17 3438.5 18 3802.5 19 4184.0 20 4741.0 21 5597.0 22 6727.0 23 8205.0 24 10001.5 25 12393.5 26 15584.5 27 19230.0 28 22503.0 29 25430.5 30 28814.5 31 33435.0 32 39909.5 33 42543.0 34 42618.5 35 45649.5 36 50078.0 37 53834.5 38 56085.5 39 57741.5 40 59936.5 41 62959.5 42 64275.0 43 65566.5 44 67672.0 45 70804.0 46 73488.5 47 87226.5 48 112094.5 49 107443.5 50 83083.0 51 72877.0 52 69909.0 53 69450.0 54 69669.5 55 65381.5 56 59055.0 57 53367.5 58 49807.0 59 44636.5 60 35765.0 61 29721.0 62 25463.5 63 19138.5 64 12902.0 65 9472.5 66 7082.5 67 5469.0 68 4423.5 69 3557.0 70 2764.5 71 1931.5 72 1452.5 73 1201.0 74 887.5 75 622.0 76 455.5 77 292.5 78 191.0 79 134.0 80 82.0 81 40.0 82 23.5 83 14.0 84 11.0 85 9.0 86 5.0 87 1.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3425560909357267 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.986586186217982E-4 16-17 0.0013006726074697857 18-19 4.563763534981704E-5 20-21 0.0 22-23 0.0 24-25 5.248328065228959E-4 26-27 0.0011181220660705174 28-29 0.0 30-31 0.0033315473805366437 32-33 0.0 34-35 0.01001746095928484 36-37 0.027770501110363666 38-39 0.021769152061862727 40-41 0.014147666958443283 42-43 0.009971823323935024 44-45 0.002646982850289388 46-47 0.0011637597014203345 48-49 5.476516241978045E-4 50-51 6.845645302472556E-5 52-53 9.127527069963408E-5 54-55 0.0 56-57 0.0 58-59 0.004563763534981704 60-61 0.01001746095928484 62-63 0.004426850628932253 64-65 0.002190606496791218 66-67 0.004107387181483534 68-69 0.00385638018705954 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2152955447627619 78-79 4.563763534981704E-4 80-81 0.0032630909275119186 82-83 7.530209832719812E-4 84-85 0.0 86-87 0.10729408070741986 88-89 0.4038930728458808 90-91 0.3905897021414091 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014832231488690537 100-101 8.899338893214322E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2191174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.92985919182629 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0701106682474 37.72380306656831 2 14.488297253491215 14.757738780992621 3 4.939844747327582 7.547567922326293 4 2.1879526253016093 4.457284764999905 5 1.1743979588025526 2.9905961338491105 6 0.7314030475888144 2.235015253570256 7 0.48987670591264487 1.746454615744069 8 0.32492308132103837 1.3238629423883845 9 0.23681525495317424 1.0854870830317447 >10 1.2199257938875776 11.387239153759934 >50 0.08709565835691462 3.0382640075820073 >100 0.040940625214526453 3.788740454854437 >500 0.004118751716669714 1.4474409719534311 >1k 0.0036710613126838443 3.682786707884388 >5k 5.372284847830016E-4 1.9657033658997576 >10k+ 8.953808079716694E-5 0.8220147745953934 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18026 0.8226640148158019 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8871 0.4048514631882269 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8716 0.39777762970900526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7846 0.3580728869546645 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6562 0.2994741631654994 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6029 0.27514930352404693 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5082 0.23193046284777022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4940 0.22544991862809616 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4451 0.20313311494203562 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4144 0.18912236088964182 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3961 0.1807706736206253 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3843 0.1753854326493469 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3644 0.16630354321473328 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3026 0.13809948456854637 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2796 0.12760282843808843 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2283 0.1041907215036323 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2202 0.10049407304029712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.563763534981704E-5 0.0 8 0.0 0.0 0.0 4.563763534981704E-5 0.0 9 0.0 0.0 0.0 4.563763534981704E-5 0.0 10-11 0.0 0.0 0.0 6.845645302472556E-5 0.0 12-13 0.0 0.0 0.0 9.127527069963408E-5 0.0 14-15 0.0 0.0 0.0 9.127527069963408E-5 0.0 16-17 0.0 0.0 0.0 9.127527069963408E-5 0.0 18-19 0.0 0.0 0.0 1.3691290604945112E-4 0.0 20-21 0.0 0.0 0.0 2.510069944239937E-4 0.0 22-23 0.0 0.0 0.0 5.248328065228959E-4 0.0 24-25 0.0 0.0 0.0 7.073833479221641E-4 0.0 26-27 0.0 0.0 0.0 9.127527069963408E-4 0.0 28-29 0.0 0.0 0.0 0.0026926204856392052 0.0 30-31 0.0 0.0 0.0 0.007826854462493623 0.0 32-33 0.0 0.0 0.0 0.018140960051552274 0.0 34-35 0.0 0.0 0.0 0.03276782218116864 0.0 36-37 0.0 0.0 0.0 0.05818798507101673 0.0 38-39 0.0 0.0 0.0 0.09577057778159105 0.0 40-41 0.0 0.0 0.0 0.13778002112109763 0.0 42-43 9.127527069963408E-5 0.0 0.0 0.18099886179737437 0.0 44-45 1.8255054139926816E-4 0.0 0.0 0.2384110070674442 0.0 46-47 1.8255054139926816E-4 0.0 0.0 0.30406074551815604 0.0 48-49 1.8255054139926816E-4 0.0 0.0 0.37790243951415997 0.0 50-51 1.8255054139926816E-4 0.0 0.0 0.46157904392804955 0.0 52-53 1.8255054139926816E-4 0.0 0.0 0.5443885332702925 0.0 54-55 1.8255054139926816E-4 0.0 0.0 0.6358463545113259 0.0 56-57 1.8255054139926816E-4 0.0 0.0 0.7531807149957055 0.0 58-59 1.8255054139926816E-4 0.0 0.0 0.8794828708263241 0.0 60-61 1.8255054139926816E-4 0.0 0.0 1.0187461150962909 0.0 62-63 1.8255054139926816E-4 0.0 0.0 1.1643758094975571 0.0 64-65 1.8255054139926816E-4 0.0 0.0 1.319771957863684 0.0 66-67 1.8255054139926816E-4 0.0 0.0 1.508118478952379 0.0 68-69 1.8255054139926816E-4 0.0 0.0 1.7269509404547516 0.0 70-71 1.8255054139926816E-4 0.0 0.0 1.9583109328606492 0.0 72-73 1.8255054139926816E-4 0.0 0.0 2.1971554974639167 0.0 74-75 1.8255054139926816E-4 0.0 0.0 2.4516081333568214 0.0 76-77 1.8255054139926816E-4 0.0 0.0 2.7183601119765024 0.0 78-79 1.8255054139926816E-4 0.0 0.0 3.0482745779203295 0.0 80-81 1.8255054139926816E-4 0.0 0.0 3.4059823637921953 0.0 82-83 1.8255054139926816E-4 0.0 0.0 3.7635532367580122 0.0 84-85 1.8255054139926816E-4 0.0 0.0 4.132328149202209 0.0 86-87 1.8255054139926816E-4 0.0 0.0 4.521639997553823 0.0 88-89 1.8255054139926816E-4 0.0 0.0 4.956612300072929 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1270 0.0 23.53613 5 GTAAGGT 1190 0.0 21.928755 4 GAGTACT 10085 0.0 21.642624 12-13 GTACCGT 180 5.2236246E-6 21.087046 6 GTACTTT 10340 0.0 20.902384 14-15 GTACATG 7770 0.0 20.52162 1 TACATGG 7405 0.0 20.375126 2 GTAAGCG 245 0.0 20.334864 94-95 AGTACTT 10350 0.0 20.01114 12-13 AGAGTAC 14080 0.0 19.798542 10-11 GGTAAGG 1315 0.0 19.122662 3 AGGTAAG 1200 0.0 18.97834 2 ACATGGG 7755 0.0 18.721378 3 ACTTTTT 12090 0.0 17.895618 16-17 TACTTTT 11525 0.0 17.74452 14-15 GGTGTGC 1805 0.0 17.611479 8 TAGACTC 1090 0.0 17.41132 5 TCTAGAC 1180 0.0 16.485422 3 CATGGGG 4670 0.0 16.255537 4 GCTATAC 735 0.0 15.492522 3 >>END_MODULE