FastQCFastQC Report
Fri 27 May 2016
SRR939218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158946
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT163010.7550443596088091No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT94800.43910315496543223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.39926890251076225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70140.3248807519965761No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT70130.324834433098373No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63140.29245752325440283No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47350.21931998299170055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.20797185293193995No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39330.18217222663281066No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38330.17754033681250017No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35400.1639688996389905No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34180.15831799405821173No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32560.1508143325493088No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30940.14331067104040582No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24290.11250860373534122No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23360.10820094620245249No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21760.10078992248995576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG77800.024.0445522
GTACATG78700.023.9527071
TAAGGTG11300.023.0506635
ACATGGG77950.022.3584123
GTAAGGT12200.021.7388944
GAGTACT88800.021.59061412-13
GTACTTT93200.020.39495714-15
AGAGTAC126550.020.36706510-11
AGTACTT92050.020.08261312-13
AGGTAAG12250.019.7166562
GGTAAGG13050.019.2342223
GTGTAGC12100.019.2286831
GTAAGCG2550.018.65290894-95
CATGGGG48050.018.6284924
TACTTTT104150.017.50066814-15
ACTTTTT110000.017.4749816-17
GAGTACG3351.4655598E-817.0088621
AAGGTGT21500.016.9527426
TAAGGTA7850.016.8926434
GGTGTGC20000.016.804138
Produced by FastQC (version 0.11.5)