##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939218_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158946 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16382577424354 33.0 31.0 34.0 31.0 34.0 2 32.2608444120418 34.0 31.0 34.0 30.0 34.0 3 32.32249440236115 34.0 31.0 34.0 30.0 34.0 4 35.75543529110964 37.0 35.0 37.0 35.0 37.0 5 35.80364724268231 37.0 35.0 37.0 35.0 37.0 6 35.89418864575585 37.0 37.0 37.0 35.0 37.0 7 35.831321394791715 37.0 36.0 37.0 35.0 37.0 8 35.82605076736519 37.0 36.0 37.0 35.0 37.0 9 37.513119364727046 39.0 38.0 39.0 35.0 39.0 10-11 37.42053877216012 39.0 38.0 39.0 35.0 39.0 12-13 37.33314450662499 39.0 37.0 39.0 34.5 39.0 14-15 38.608814671603646 40.0 38.0 41.0 34.0 41.0 16-17 38.53273356536013 40.0 38.0 41.0 34.0 41.0 18-19 38.44917149386784 40.0 38.0 41.0 34.0 41.0 20-21 38.43674922855875 40.0 38.0 41.0 34.0 41.0 22-23 38.484380109553456 40.0 38.0 41.0 34.0 41.0 24-25 38.420869720687776 40.0 38.0 41.0 34.0 41.0 26-27 38.29518501157509 40.0 38.0 41.0 34.0 41.0 28-29 38.16218099016835 40.0 38.0 41.0 34.0 41.0 30-31 37.93587565413864 40.0 38.0 41.0 33.0 41.0 32-33 37.70805175303134 40.0 37.5 41.0 33.0 41.0 34-35 37.37141642264327 40.0 37.0 41.0 31.5 41.0 36-37 37.08939501034301 40.0 37.0 41.0 31.0 41.0 38-39 36.881200363510715 40.0 36.5 41.0 30.5 41.0 40-41 36.696201757709545 40.0 36.0 41.0 30.0 41.0 42-43 36.46463552122193 40.0 36.0 41.0 30.0 41.0 44-45 36.16016079142322 39.0 35.5 41.0 29.5 41.0 46-47 35.96233022965836 39.5 35.0 41.0 28.5 41.0 48-49 35.783819511928506 40.0 35.0 41.0 27.0 41.0 50-51 34.63385976305105 38.0 34.0 40.0 25.5 40.5 52-53 34.77740295496043 38.5 34.5 39.5 25.5 40.5 54-55 35.30855357197447 39.0 35.0 41.0 26.0 41.0 56-57 35.184326055399254 39.0 35.0 41.0 26.0 41.0 58-59 34.967600625490405 39.0 35.0 41.0 26.0 41.0 60-61 34.636822088185625 38.0 35.0 40.5 24.5 41.0 62-63 34.275747054349665 38.0 34.0 40.0 23.0 41.0 64-65 33.90876172910299 37.0 34.0 40.0 22.5 41.0 66-67 33.50244054274632 37.0 34.0 39.5 21.5 41.0 68-69 33.080152768990054 36.0 33.0 39.0 20.0 41.0 70-71 32.61934388354317 35.5 33.0 39.0 18.5 40.5 72-73 32.17531193461995 35.0 32.5 38.0 17.0 40.0 74-75 31.748524743092233 35.0 32.0 37.0 12.5 39.5 76-77 31.325562566178128 35.0 32.0 37.0 9.0 39.0 78-79 30.89404112006507 35.0 31.0 36.0 7.0 39.0 80-81 30.42949707866709 35.0 31.0 36.0 2.0 37.5 82-83 30.022660131378924 34.5 30.5 35.0 2.0 37.0 84-85 29.638675770491712 34.0 30.0 35.0 2.0 37.0 86-87 29.268460165284356 34.0 30.0 35.0 2.0 36.0 88-89 28.825819867657643 34.0 29.0 35.0 2.0 36.0 90-91 28.399078068650166 34.0 29.0 35.0 2.0 35.5 92-93 27.977763686539635 34.0 28.5 35.0 2.0 35.0 94-95 27.47783108053652 34.0 27.0 35.0 2.0 35.0 96-97 26.86940432970533 34.0 25.5 35.0 2.0 35.0 98-99 26.164235464898148 33.0 24.5 35.0 2.0 35.0 100-101 23.197493823374927 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1939699119851639 1101 2 0.29927600046591607 1101 3 0.22211064759768107 1101 4 0.33143368570957676 1101 5 0.30207286949654133 1101 6 0.29277896022038874 1101 7 0.23193421764482736 1101 8 0.28568975140488817 1101 9 0.3049114208803232 1101 10-11 0.3302421496041461 1101 12-13 0.24292164953617146 1101 14-15 0.3361133406106802 1101 16-17 0.36673659269956715 1101 18-19 0.42173127565349944 1101 20-21 0.3519251561974315 1101 22-23 0.39150439623350053 1101 24-25 0.4784061243618538 1101 26-27 0.32906157995201113 1101 28-29 0.283214388882179 1101 30-31 0.29452981446018356 1101 32-33 0.30279955166766825 1101 34-35 0.4370436577376253 1101 36-37 0.42999544325990513 1101 38-39 0.33697943931777274 1101 40-41 0.4170956103242034 1101 42-43 0.3496403907381449 1101 44-45 0.2098384146343193 1101 46-47 0.4884951747868129 1101 48-49 0.5584685810844761 1101 50-51 0.4426675959264088 1101 52-53 0.44871456391688724 1101 54-55 0.4513804763372562 1101 56-57 0.28763653127098365 1101 58-59 0.28274458925135093 1101 60-61 0.192183740174535 1101 62-63 0.10774880169649492 1101 64-65 0.1776599150530771 1101 66-67 0.3117629711391672 1101 68-69 0.28204597972726475 1101 70-71 0.26787226071211023 1101 72-73 0.3070708326445484 1101 74-75 0.13465633203425753 1101 76-77 0.09910300492186508 1101 78-79 -0.037970732908267024 1101 80-81 -0.07510708498416463 1101 82-83 -0.2750158795563884 1101 84-85 -0.34733352924286365 1101 86-87 -0.34934275269034387 1101 88-89 -0.3982791838838189 1101 90-91 -0.5541033591283231 1101 92-93 -0.6038690678879881 1101 94-95 -0.8384768036368513 1101 96-97 -0.9615599747321788 1101 98-99 -0.8025682509852565 1101 100-101 -1.0299615751976 1102 1 0.3560947682196627 1102 2 0.34654248792126907 1102 3 0.3421978603615301 1102 4 0.17275635957879842 1102 5 0.2876725893971539 1102 6 0.3662709464393643 1102 7 0.29173327702697804 1102 8 0.28590488455866847 1102 9 0.41206478296818005 1102 10-11 0.44397710840060967 1102 12-13 0.46464862027095677 1102 14-15 0.4556092924413164 1102 16-17 0.532445248981297 1102 18-19 0.6263094057911545 1102 20-21 0.5730583082732608 1102 22-23 0.5725814318066327 1102 24-25 0.552861094321365 1102 26-27 0.5146561275627946 1102 28-29 0.5739500789040051 1102 30-31 0.7723689681826258 1102 32-33 0.7410974022402144 1102 34-35 0.6721098786779578 1102 36-37 0.7041266619792736 1102 38-39 0.7351732870779557 1102 40-41 0.8401326481822338 1102 42-43 0.7690478736774011 1102 44-45 0.7101216117119193 1102 46-47 0.896424152995273 1102 48-49 0.9525910978524976 1102 50-51 0.8359760454302219 1102 52-53 0.8308856808842435 1102 54-55 0.9106309319676811 1102 56-57 0.8792100754063839 1102 58-59 0.8085034451635735 1102 60-61 0.8268551050991917 1102 62-63 0.861021340730936 1102 64-65 0.8433427098856825 1102 66-67 0.9560306388428472 1102 68-69 0.8748693991735408 1102 70-71 0.8687570633025672 1102 72-73 0.8852022491613738 1102 74-75 0.7528079147975753 1102 76-77 0.6443702034249696 1102 78-79 0.6836607493989497 1102 80-81 0.7636389643952874 1102 82-83 0.6754907064452915 1102 84-85 0.5227962412353513 1102 86-87 0.6896970941189373 1102 88-89 0.5913346023715889 1102 90-91 0.6402165856475364 1102 92-93 0.5023325360334034 1102 94-95 0.295750795872177 1102 96-97 0.33574338660256586 1102 98-99 0.32180983089663684 1102 100-101 0.015344415479205509 1103 1 0.1769716210732355 1103 2 0.23888454114934632 1103 3 0.24216609952065937 1103 4 0.226383381437536 1103 5 0.2301297756733831 1103 6 0.23474301840413148 1103 7 0.2618466566120361 1103 8 0.24663640950912225 1103 9 0.27439587429493173 1103 10-11 0.3330092243453251 1103 12-13 0.31877772257505654 1103 14-15 0.4161733285825093 1103 16-17 0.36273442151213686 1103 18-19 0.37273727614957863 1103 20-21 0.3374998413072916 1103 22-23 0.34607425308463036 1103 24-25 0.3434293652413416 1103 26-27 0.4046220453132463 1103 28-29 0.38717400275739067 1103 30-31 0.4847626989455378 1103 32-33 0.37974546565602196 1103 34-35 0.3518190298805166 1103 36-37 0.27677255507096277 1103 38-39 0.3048210615583429 1103 40-41 0.1873264731248625 1103 42-43 0.21350031347240872 1103 44-45 0.34013386941144574 1103 46-47 0.4751142554129615 1103 48-49 0.4483840270952939 1103 50-51 0.3722925819105214 1103 52-53 0.30438454003043347 1103 54-55 0.3971037637664594 1103 56-57 0.4288958029887979 1103 58-59 0.3149055153528266 1103 60-61 0.3980808585093385 1103 62-63 0.26102817813417545 1103 64-65 0.26049419806854957 1103 66-67 0.1740628838159779 1103 68-69 0.13346702019833145 1103 70-71 0.08508478224560179 1103 72-73 0.14922058001889837 1103 74-75 0.17795351288839356 1103 76-77 0.009396692848504529 1103 78-79 0.010806232797843762 1103 80-81 0.024363624372323756 1103 82-83 -0.034742901156135986 1103 84-85 -0.028207202830827782 1103 86-87 -0.2156452612782509 1103 88-89 -0.25100370131277927 1103 90-91 -0.2384699602176532 1103 92-93 -0.0980652188721578 1103 94-95 -0.2111183130708163 1103 96-97 -0.24893523338889878 1103 98-99 -0.3535248985903259 1103 100-101 -0.4920609333551802 1104 1 0.09369598781491106 1104 2 0.27088404108203434 1104 3 0.2477245400799717 1104 4 0.16245650666593292 1104 5 0.22911040768153867 1104 6 0.27348861877341335 1104 7 0.23061168267323495 1104 8 0.13042365009920331 1104 9 0.1607171098458693 1104 10-11 0.23359920761229347 1104 12-13 0.26014723385541316 1104 14-15 0.3152006014846265 1104 16-17 0.26713260972978503 1104 18-19 0.2631047294606006 1104 20-21 0.2741884340024612 1104 22-23 0.25051812052832645 1104 24-25 0.20049906693194686 1104 26-27 0.27290960325373703 1104 28-29 0.2741107057751009 1104 30-31 0.29873284877339046 1104 32-33 0.24688383043589823 1104 34-35 0.2583184624964403 1104 36-37 0.18152656041783644 1104 38-39 0.20373053014065334 1104 40-41 0.05887408530342242 1104 42-43 0.15166987475950577 1104 44-45 0.2686891556999882 1104 46-47 0.3177204716343809 1104 48-49 0.42498424250890565 1104 50-51 0.14901733331937095 1104 52-53 0.11565961552135917 1104 54-55 0.10977902774430248 1104 56-57 0.1365511604407672 1104 58-59 0.2654805927670907 1104 60-61 0.3501056503082367 1104 62-63 0.20240009358433753 1104 64-65 0.2446771650064079 1104 66-67 0.1660449775593662 1104 68-69 0.12291511898086327 1104 70-71 0.08860929516079352 1104 72-73 0.20754573917493957 1104 74-75 0.0918457676149913 1104 76-77 0.01248605103133471 1104 78-79 0.03736750066082806 1104 80-81 0.0322949805164221 1104 82-83 -0.16480848690806127 1104 84-85 -0.09721168420331949 1104 86-87 -0.10519048852038182 1104 88-89 -0.07901420272643023 1104 90-91 -0.0502282738367974 1104 92-93 -0.0771850042278821 1104 94-95 -0.16594877101707795 1104 96-97 -0.07717893927237895 1104 98-99 -0.023417961117974784 1104 100-101 -0.3091709289315112 1105 1 0.14892501495138077 1105 2 0.2444776051655566 1105 3 0.2124488793119994 1105 4 0.16124855817889028 1105 5 0.2364089895045467 1105 6 0.29018806744671366 1105 7 0.36343667532376145 1105 8 0.38296186606572036 1105 9 0.41580064583865806 1105 10-11 0.38592019434810965 1105 12-13 0.32836881128734063 1105 14-15 0.3764654961146263 1105 16-17 0.382007580861476 1105 18-19 0.4043685701064632 1105 20-21 0.40803912371624307 1105 22-23 0.4560330150291634 1105 24-25 0.4872327390901816 1105 26-27 0.5008959303928506 1105 28-29 0.4557435597006503 1105 30-31 0.5855831566762646 1105 32-33 0.4952365927268261 1105 34-35 0.2984022800683235 1105 36-37 0.5444207905263312 1105 38-39 0.44605104155029096 1105 40-41 0.38767438269228194 1105 42-43 0.3129330644151693 1105 44-45 0.42872011390072373 1105 46-47 0.4646401771594739 1105 48-49 0.5416179205053879 1105 50-51 0.3812675171207687 1105 52-53 0.4054189713097216 1105 54-55 0.45745532854930815 1105 56-57 0.5124101704293267 1105 58-59 0.5776360781603671 1105 60-61 0.6053439256388558 1105 62-63 0.5993704316807467 1105 64-65 0.5704879448875033 1105 66-67 0.7071998290121897 1105 68-69 0.6422364823610138 1105 70-71 0.5752762666562035 1105 72-73 0.7864970301246856 1105 74-75 0.6183355147776304 1105 76-77 0.4422399457352171 1105 78-79 0.3274546765154973 1105 80-81 0.21330306467809024 1105 82-83 0.15713200122092275 1105 84-85 0.1269415506238296 1105 86-87 0.10358374859401565 1105 88-89 0.1923362092832015 1105 90-91 0.23094550283120086 1105 92-93 0.17967364254948492 1105 94-95 0.09014289563667077 1105 96-97 0.04222299706181332 1105 98-99 0.07477603189576598 1105 100-101 0.017240208580869165 1106 1 0.132753371045645 1106 2 0.07136274924820896 1106 3 0.21932598035482442 1106 4 0.2443410113614064 1106 5 0.2560042101873421 1106 6 0.2170336506451136 1106 7 0.30785791116350936 1106 8 0.25615312582822014 1106 9 0.3213450081358218 1106 10-11 0.3257958292222938 1106 12-13 0.3638648901795989 1106 14-15 0.35356988601382255 1106 16-17 0.34523193889049253 1106 18-19 0.286355835125633 1106 20-21 0.4336876378426595 1106 22-23 0.4988934078898808 1106 24-25 0.48258030863756574 1106 26-27 0.4583156346324557 1106 28-29 0.3504312023601699 1106 30-31 0.4281313406756482 1106 32-33 0.36555314523231885 1106 34-35 0.2932981206892933 1106 36-37 0.3679261465969432 1106 38-39 0.4037673265919963 1106 40-41 0.46875922166287154 1106 42-43 0.5935069337968031 1106 44-45 0.48375353177490155 1106 46-47 0.4221654525882883 1106 48-49 0.4656382475520999 1106 50-51 0.553523962248164 1106 52-53 0.5716111758022535 1106 54-55 0.5366408927524375 1106 56-57 0.5940591043238967 1106 58-59 0.6377566683926545 1106 60-61 0.5705816860498913 1106 62-63 0.5344199909996732 1106 64-65 0.47740636843156636 1106 66-67 0.4049329047803383 1106 68-69 0.474648196409305 1106 70-71 0.4757666756583774 1106 72-73 0.4814505259529156 1106 74-75 0.5672561720520299 1106 76-77 0.5259612961807321 1106 78-79 0.5570381648291836 1106 80-81 0.5549412476758668 1106 82-83 0.5318741130775635 1106 84-85 0.5961721417570551 1106 86-87 0.5780902567621276 1106 88-89 0.6599253508774439 1106 90-91 0.5165228677642482 1106 92-93 0.4487321992013271 1106 94-95 0.6438138286041273 1106 96-97 0.6094312015664158 1106 98-99 0.7398216753917985 1106 100-101 0.42780555676949916 1107 1 0.0768597371661528 1107 2 0.116251607127559 1107 3 0.006344282461441253 1107 4 0.07800201570730536 1107 5 0.12404486496864564 1107 6 0.1442108787681775 1107 7 0.09592756411259984 1107 8 0.1466506346046117 1107 9 0.06282834948172678 1107 10-11 0.15069167380587345 1107 12-13 0.15559897870142692 1107 14-15 0.10632729254155748 1107 16-17 0.009376239660944918 1107 18-19 0.016529030840331416 1107 20-21 0.11768478772609825 1107 22-23 0.15146233588477287 1107 24-25 0.20111863467675306 1107 26-27 0.27412470167327285 1107 28-29 0.18330871138506666 1107 30-31 0.18270591155724247 1107 32-33 0.08831790165822184 1107 34-35 0.20769737403904998 1107 36-37 0.1877771797403014 1107 38-39 0.26615553693480365 1107 40-41 0.22342769729016965 1107 42-43 0.3158786283736532 1107 44-45 0.38348333460838546 1107 46-47 0.45523115341398324 1107 48-49 0.4774917504141669 1107 50-51 0.23575436482951062 1107 52-53 0.4336727505537752 1107 54-55 0.4485216829477352 1107 56-57 0.23835796322592984 1107 58-59 0.3177555788108748 1107 60-61 0.2799058777283534 1107 62-63 0.27630451826932756 1107 64-65 0.32916231549318553 1107 66-67 0.19984183390305077 1107 68-69 0.1232231859196915 1107 70-71 0.202857979744401 1107 72-73 0.31612584491842455 1107 74-75 0.1158266694269976 1107 76-77 0.2032032779579538 1107 78-79 0.15844859977302406 1107 80-81 0.00888777979511346 1107 82-83 0.1780857136827585 1107 84-85 0.08163172066236868 1107 86-87 -0.07055307055301441 1107 88-89 -0.21662790052060998 1107 90-91 -0.05636704720058461 1107 92-93 -0.19689255647907444 1107 94-95 0.042257877145953415 1107 96-97 0.030826238999285493 1107 98-99 -0.13149991084811674 1107 100-101 -0.21935273748066209 1108 1 0.04850587203514323 1108 2 0.16312589647775155 1108 3 0.19341120241614362 1108 4 0.25527592282838896 1108 5 0.24903722727773214 1108 6 0.22011850060944482 1108 7 0.06912214031837749 1108 8 0.18346338598801282 1108 9 0.1357318894653261 1108 10-11 0.23439755034863907 1108 12-13 0.28357773558396104 1108 14-15 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-1.6104535997126739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6262.0 3 1668.0 4 1623.0 5 1905.0 6 2146.0 7 2677.0 8 3266.0 9 4172.0 10 5279.0 11 6476.0 12 8756.0 13 13965.0 14 12570.0 15 12986.0 16 15943.0 17 22030.0 18 18625.0 19 13335.0 20 12747.0 21 12689.0 22 13547.0 23 15074.0 24 16661.0 25 18644.0 26 21027.0 27 24443.0 28 28812.0 29 34500.0 30 41449.0 31 51344.0 32 64412.0 33 84011.0 34 118407.0 35 178082.0 36 288960.0 37 456709.0 38 439782.0 39 83913.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.171119066095756 18.547477045984284 14.73925158772928 22.542152300190683 2 15.120383595184656 20.48482684424329 40.231770881638255 24.1630186789338 3 20.43826219865648 23.857252694968434 29.9491844375833 25.755300668791786 4 12.693937540204372 16.398838496575642 37.85214623515482 33.05507772806517 5 13.440725815241212 37.548703911293366 34.395895259169045 14.614675014296374 6 31.54320014832669 36.1494391397052 17.260081579679245 15.047279132288866 7 27.98564629454764 33.05533575034083 20.825203630795848 18.13381432431568 8 25.835463721593214 34.022783372541745 19.848419881563547 20.29333302430149 9 25.56912102959568 17.839041308614483 21.119245863066073 35.472591798723755 10-11 25.084399050484606 26.146647377220994 27.075512061079742 21.693441511214655 12-13 26.79522053301737 23.72340800323378 27.350609207742526 22.13076225600632 14-15 22.79936524285116 25.371095836787593 26.94053682729428 24.889002093066967 16-17 21.63390158283839 28.341461447430085 27.645229490012408 22.379407479719116 18-19 21.485028535507944 27.446147370333918 29.596497579894095 21.472326514264044 20-21 22.47963821042011 25.877430359423048 29.57281209995246 22.07011933020438 22-23 21.894597220293704 25.913713061633892 29.868478913208413 22.323210804863987 24-25 22.507216035944552 25.958324349513482 29.777038979345328 21.757420635196638 26-27 21.822806057627215 25.77619144772839 30.503148445252293 21.897854049392105 28-29 21.35603059303387 27.507788862535126 29.62746761296024 21.50871293147076 30-31 21.741389022176786 26.305259424613524 29.319513116688338 22.633838436521348 32-33 21.530734366129995 26.355484768710213 29.735721469886627 22.378059395273162 34-35 22.777374344841046 26.294824142614644 29.25266275420632 21.67513875833799 36-37 22.48576684422654 25.74461412449649 29.94407317959608 21.82554585168089 38-39 21.77544905440423 26.598075940032345 29.693387859539154 21.93308714602427 40-41 22.106487972716515 26.727855148989843 29.30228607399633 21.86337080429731 42-43 21.68976026175948 26.80837235547221 28.61653520652015 22.88533217624816 44-45 22.18391808066452 26.31580662771843 28.991617864970916 22.508657426646135 46-47 23.001448463608924 27.128568721473624 28.35871520207022 21.511267612847234 48-49 22.501873572993915 27.014606070073842 28.380597350247445 22.102923006684797 50-51 21.725979629471404 27.45842396485666 29.000799241407453 21.81479716426448 52-53 22.580114960457607 27.577402750390533 27.86587480997069 21.97660747918117 54-55 21.839573611694714 27.08415442259141 28.397889475662975 22.6783824900509 56-57 22.468617425948203 27.018628119726568 28.03578215039665 22.476972303928587 58-59 23.0338570299965 26.746855900226535 27.887981737518718 22.331305332258246 60-61 22.044898371175808 27.15201599380045 29.05704768695987 21.746037948063872 62-63 21.69032613358403 27.325398301378367 28.92528397062873 22.058991594408873 64-65 21.496140025414974 28.040658367919207 28.224029860962684 22.239171745703132 66-67 21.821772689266307 27.301373381184234 27.80124194099325 23.075611988556204 68-69 21.865021856871085 27.044999002112913 28.370547030756114 22.719432110259884 70-71 22.563045581162264 27.029341863141475 27.744084331833506 22.663528223862752 72-73 22.216722112737447 26.727994180956603 28.713412112700194 22.34187159360576 74-75 21.693349117099466 28.301342023718494 27.774228189669703 22.23108066951234 76-77 22.08889304815075 27.122236205412285 27.805534642848993 22.983336103587966 78-79 22.223388454486763 27.387208919675356 27.994027235841802 22.395375389996087 80-81 22.761091812569457 27.094663710789536 27.68644427764298 22.457800198998026 82-83 22.377522267885013 27.06766899791317 28.374051585375646 22.18075714882617 84-85 22.085656806226726 27.718995733662677 27.98981702629143 22.205530433819167 86-87 22.215159024369353 27.736960718636805 27.698647202345178 22.349233054648668 88-89 21.97003875054066 27.763949938566217 27.092391848372387 23.173619462520737 90-91 22.272190699266346 27.15543675915556 27.369084013010692 23.203288528567402 92-93 22.747700706616943 27.28269730357658 27.706476534156486 22.26312545564999 94-95 22.324848987776296 26.91527598978405 27.992100308020003 22.767774714419662 96-97 21.79138069092407 26.714707719732633 28.675893796193954 22.81801779314934 98-99 22.050641328927416 27.340254519850838 27.445194596668742 23.16390955455301 100-101 22.174844800598915 26.062139354024104 28.261003164978337 23.502012680398643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1988.0 1 1128.0 2 353.0 3 447.0 4 404.5 5 419.5 6 738.0 7 1537.5 8 2493.5 9 3014.5 10 2793.0 11 2455.5 12 2545.5 13 2792.5 14 3103.5 15 3411.0 16 3765.0 17 4008.5 18 4164.5 19 4528.5 20 5090.5 21 5706.0 22 6664.5 23 8111.0 24 9798.0 25 12071.5 26 15313.5 27 18916.5 28 21988.5 29 24649.5 30 27634.0 31 32088.0 32 38189.5 33 41132.0 34 41732.0 35 44665.0 36 48852.5 37 52547.0 38 55068.0 39 56662.5 40 58502.5 41 61361.5 42 63062.5 43 64285.5 44 65680.5 45 69030.0 46 72561.0 47 84629.5 48 108335.5 49 105232.5 50 81991.5 51 71646.5 52 68857.0 53 68707.0 54 68779.0 55 64411.0 56 58132.0 57 52732.0 58 49060.5 59 44108.0 60 35814.5 61 29805.0 62 25349.0 63 19279.5 64 13219.0 65 9737.0 66 7344.5 67 5699.0 68 4601.5 69 3734.0 70 2935.5 71 2148.5 72 1625.0 73 1256.0 74 938.5 75 662.5 76 486.0 77 340.5 78 242.0 79 177.0 80 122.0 81 84.0 82 60.0 83 50.0 84 37.5 85 24.5 86 17.0 87 13.5 88 11.5 89 10.5 90 9.0 91 8.0 92 6.0 93 2.0 94 2.5 95 4.0 96 3.5 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3813434889061607 2 0.11607515889698028 3 0.11672362347182376 4 0.12853494251361544 5 0.1299245094597086 6 0.07160901662199981 7 0.08027065058598039 8 0.07739887889738789 9 0.07744519779559099 10-11 0.07573139856207613 12-13 0.07957586711293381 14-15 0.07343861310102244 16-17 0.07499029619082645 18-19 0.08379088684941634 20-21 0.08247079825062785 22-23 0.09224408577148294 24-25 0.10611659578331278 26-27 0.10773775722042143 28-29 0.10099835753186971 30-31 0.09083135937628824 32-33 0.08904808179546872 34-35 0.33690050608028177 36-37 0.5283828312519164 38-39 0.508952053455714 40-41 0.4814154684739684 42-43 0.5104342581982134 44-45 0.5198601539825451 46-47 0.5165946716592263 48-49 0.5239362170244184 50-51 0.5226856067729346 52-53 0.5211339236831306 54-55 0.5159230476352813 56-57 0.4863715905817005 58-59 0.4760887951806113 60-61 0.42210411932489283 62-63 0.49989670885700704 64-65 0.5277112072279714 66-67 0.5364423195392567 68-69 0.551634918149875 70-71 0.5469798688804629 72-73 0.5333157939105471 74-75 0.5369286679703892 76-77 0.5154830181023519 78-79 0.48759904138408283 80-81 0.4948479489528687 82-83 0.507354051467707 84-85 0.5025832049527872 86-87 0.5039032935515757 88-89 0.5143713645454773 90-91 0.5317872702698446 92-93 0.5373686975033187 94-95 0.5336168667488672 96-97 0.534821158102148 98-99 0.5340568962817968 100-101 0.51247228971915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2158946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.4202115635028 #Duplication Level Percentage of deduplicated Percentage of total 1 75.24443786540694 39.44329351881473 2 13.820205217927937 14.489161627496156 3 4.805010620212554 7.556390198292597 4 2.0899248501964793 4.382172111964853 5 1.1367603085451414 2.979460793546451 6 0.659131505866499 2.0731087791415237 7 0.44202163873976325 1.6219607472869217 8 0.30566581659391623 1.2818453418867144 9 0.2194824480352955 1.035478472443713 >10 1.1548454878190637 11.081855092521605 >50 0.07708599180199352 2.7619311804414783 >100 0.03746148474395522 3.6229255116865087 >500 0.003894862453982351 1.4269128909898112 >1k 0.003540784049074844 3.703356568238312 >5k 4.425980061343555E-4 1.7837455186022921 >10k+ 8.851960122687109E-5 0.7564016466464443 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16301 0.7550443596088091 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9480 0.43910315496543223 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8620 0.39926890251076225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7014 0.3248807519965761 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7013 0.324834433098373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6314 0.29245752325440283 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4735 0.21931998299170055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4490 0.20797185293193995 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3933 0.18217222663281066 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3833 0.17754033681250017 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3540 0.1639688996389905 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3418 0.15831799405821173 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3256 0.1508143325493088 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3094 0.14331067104040582 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2429 0.11250860373534122 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2336 0.10820094620245249 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2176 0.10078992248995576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3159449101552333E-5 0.0 12-13 0.0 0.0 0.0 6.9478347304657E-5 0.0 14-15 0.0 0.0 0.0 1.8527559281241866E-4 0.0 16-17 0.0 0.0 0.0 1.8527559281241866E-4 0.0 18-19 0.0 0.0 0.0 2.0843504191397097E-4 0.0 20-21 0.0 0.0 0.0 2.5475394011707564E-4 0.0 22-23 0.0 0.0 0.0 3.7055118562483733E-4 0.0 24-25 0.0 0.0 0.0 7.179429221481222E-4 0.0 26-27 0.0 0.0 0.0 0.001111653556874512 0.0 28-29 0.0 0.0 0.0 0.003126525628709565 0.0 30-31 2.3159449101552333E-5 0.0 0.0 0.008870069005894543 0.0 32-33 4.6318898203104666E-5 0.0 0.0 0.019824488430928797 0.0 34-35 4.6318898203104666E-5 0.0 0.0 0.035572913819984384 0.0 36-37 4.6318898203104666E-5 0.0 0.0 0.06097882948438729 0.0 38-39 4.6318898203104666E-5 0.0 0.0 0.09997934177140141 0.0 40-41 6.9478347304657E-5 0.0 0.0 0.13962831863325903 0.0 42-43 1.1579724550776167E-4 0.0 0.0 0.18502083887230159 0.0 44-45 1.38956694609314E-4 0.0 0.0 0.24396163683575225 0.0 46-47 1.38956694609314E-4 0.0 0.0 0.31471375384099465 0.0 48-49 1.38956694609314E-4 0.0 0.0 0.38852291812764195 0.0 50-51 1.38956694609314E-4 0.0 0.0 0.4732170234920188 0.0 52-53 1.38956694609314E-4 0.0 0.0 0.5592312174551841 0.0 54-55 1.38956694609314E-4 0.0 0.0 0.654323915466158 0.0 56-57 1.38956694609314E-4 0.0 0.0 0.7790607083271188 0.0 58-59 1.38956694609314E-4 0.0 0.0 0.9063913594874535 0.0 60-61 1.38956694609314E-4 0.0 0.0 1.0458344025279003 0.0 62-63 1.38956694609314E-4 0.0 0.0 1.1872459987419788 0.0 64-65 1.38956694609314E-4 0.0 0.0 1.3427153805606995 0.0 66-67 1.38956694609314E-4 0.0 0.0 1.5287089162952663 0.0 68-69 1.38956694609314E-4 0.0 0.0 1.744508663023531 0.0 70-71 1.6211614371086633E-4 0.0 0.0 1.9709154374403066 0.0 72-73 2.0843504191397097E-4 0.0 0.0 2.203691060360009 0.0 74-75 2.315944910155233E-4 0.0 0.0 2.4558048232795078 0.0 76-77 2.315944910155233E-4 0.0 0.0 2.7285536553484895 0.0 78-79 2.315944910155233E-4 0.0 0.0 3.065986828758107 0.0 80-81 2.77913389218628E-4 0.0 0.0 3.4271352780477145 0.0 82-83 2.77913389218628E-4 0.0 0.0 3.7828412567984566 0.0 84-85 2.77913389218628E-4 0.0 0.0 4.150497511285599 0.0 86-87 2.77913389218628E-4 0.0 0.0 4.537098195137812 0.0 88-89 2.77913389218628E-4 0.0 0.0 4.966335424785983 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 7780 0.0 24.044552 2 GTACATG 7870 0.0 23.952707 1 TAAGGTG 1130 0.0 23.050663 5 ACATGGG 7795 0.0 22.358412 3 GTAAGGT 1220 0.0 21.738894 4 GAGTACT 8880 0.0 21.590614 12-13 GTACTTT 9320 0.0 20.394957 14-15 AGAGTAC 12655 0.0 20.367065 10-11 AGTACTT 9205 0.0 20.082613 12-13 AGGTAAG 1225 0.0 19.716656 2 GGTAAGG 1305 0.0 19.234222 3 GTGTAGC 1210 0.0 19.228683 1 GTAAGCG 255 0.0 18.652908 94-95 CATGGGG 4805 0.0 18.628492 4 TACTTTT 10415 0.0 17.500668 14-15 ACTTTTT 11000 0.0 17.47498 16-17 GAGTACG 335 1.4655598E-8 17.008862 1 AAGGTGT 2150 0.0 16.952742 6 TAAGGTA 785 0.0 16.892643 4 GGTGTGC 2000 0.0 16.80413 8 >>END_MODULE