FastQCFastQC Report
Fri 27 May 2016
SRR939218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158946
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT177340.8214193407338581No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT87330.404502938007713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.39019039846295367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.34989295702625267No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT64980.3009802005237741No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57360.26568520009300833No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48580.22501720747068243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46530.215521833339046No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43920.20343260090803567No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39620.18351547468070067No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39440.18268173451304479No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38210.17698451003406293No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA35480.16433945082461535No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28740.13312051343572281No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27620.12793279683697506No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22410.10380065087315755No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22080.1022721272324551No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21640.10023409571151848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG5100.030.2418694-95
GTACCGA1252.2708502E-422.77916
TACATGG79800.022.5388432
GTACATG83350.022.3563541
GAGTACT97450.022.10952412-13
ACATGGG80450.021.5898883
GTACTTT100400.021.43625814-15
AGTACTT99250.020.99130212-13
AGAGTAC138950.020.33895310-11
CATGGGG46150.018.8180544
GTAAGGT11700.018.6580944
AGGTAAG10700.018.6277682
TACTTTT111900.018.46985214-15
TAAGGTG14050.018.2394925
ACTTTTT118850.018.12808816-17
GGTAAGG12100.017.2568953
GTGTACG2555.3399763E-616.8202381
CGCCGAT850.001799644816.7493448-49
CTAGACT13300.016.4135134
GTGTAGC13100.016.0070461
Produced by FastQC (version 0.11.5)