##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939218_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158946 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45869049063756 34.0 31.0 34.0 31.0 34.0 2 32.681189802801924 34.0 31.0 34.0 31.0 34.0 3 32.7838996436224 34.0 31.0 34.0 31.0 34.0 4 36.23860670901449 37.0 35.0 37.0 35.0 37.0 5 36.203993985954256 37.0 35.0 37.0 35.0 37.0 6 36.25133560542969 37.0 37.0 37.0 35.0 37.0 7 36.2073284834359 37.0 37.0 37.0 35.0 37.0 8 36.182485342384666 37.0 37.0 37.0 35.0 37.0 9 37.917484272418115 39.0 38.0 39.0 35.0 39.0 10-11 37.834376820911686 39.0 38.0 39.0 35.0 39.0 12-13 37.7493658942836 39.0 38.0 39.0 35.0 39.0 14-15 39.12440561273881 40.5 38.5 41.0 36.0 41.0 16-17 39.24359293840605 41.0 39.0 41.0 36.0 41.0 18-19 39.29368937435211 41.0 39.0 41.0 36.0 41.0 20-21 39.25748513394962 41.0 39.0 41.0 36.0 41.0 22-23 39.18453726957506 41.0 39.0 41.0 36.0 41.0 24-25 39.09532035539564 40.5 39.0 41.0 35.0 41.0 26-27 38.92843012284698 40.0 38.0 41.0 35.0 41.0 28-29 38.77957114258532 40.0 38.0 41.0 35.0 41.0 30-31 38.568502871308496 40.0 38.0 41.0 34.5 41.0 32-33 38.42663642351407 40.0 38.0 41.0 34.0 41.0 34-35 38.28285237333402 40.0 38.0 41.0 34.0 41.0 36-37 38.10029732100757 40.0 38.0 41.0 33.5 41.0 38-39 37.942333898115095 40.0 38.0 41.0 33.0 41.0 40-41 37.75430881550534 40.0 37.0 41.0 33.0 41.0 42-43 37.51987752356938 40.0 37.0 41.0 33.0 41.0 44-45 37.229518477998056 40.0 37.0 41.0 32.0 41.0 46-47 36.877729456873865 40.0 36.5 41.0 31.0 41.0 48-49 36.463499318649006 39.5 36.0 41.0 30.0 41.0 50-51 36.154269027571786 39.0 35.5 41.0 30.0 41.0 52-53 35.874670556836534 39.0 35.0 40.5 29.0 41.0 54-55 35.600672272488524 39.0 35.0 40.0 28.0 41.0 56-57 35.303014526532856 38.5 35.0 40.0 27.5 41.0 58-59 34.92957072571523 38.0 34.5 40.0 26.5 41.0 60-61 34.495530457917894 38.0 34.0 40.0 26.0 41.0 62-63 34.08597644406113 37.0 34.0 40.0 25.5 41.0 64-65 33.61540284008956 36.5 33.0 39.0 23.5 41.0 66-67 33.1472883064236 36.0 33.0 39.0 22.5 40.0 68-69 32.624133489211864 35.5 32.0 38.5 21.5 40.0 70-71 32.0666468730575 35.0 31.5 38.0 19.5 40.0 72-73 31.45727197438009 35.0 31.0 37.0 17.5 39.0 74-75 30.900911370641047 34.5 30.5 36.5 13.0 39.0 76-77 28.94206270096612 32.5 28.0 34.5 8.0 36.5 78-79 29.9257385316724 34.0 29.5 35.0 7.5 37.0 80-81 29.927619773722917 34.0 30.0 35.0 6.5 37.0 82-83 29.50480072220426 34.0 29.5 35.0 2.0 37.0 84-85 28.99147130127386 34.0 29.0 35.0 2.0 36.0 86-87 28.277100260960673 33.5 28.0 35.0 2.0 36.0 88-89 27.535664162049443 33.0 26.5 35.0 2.0 35.0 90-91 26.917752922027695 32.5 25.5 34.5 2.0 35.0 92-93 26.421522817152443 32.0 25.0 34.0 2.0 35.0 94-95 25.81067312475625 32.0 23.5 34.0 2.0 35.0 96-97 24.903615699512635 31.5 19.0 34.0 2.0 35.0 98-99 23.59364870635949 31.0 2.0 34.0 2.0 35.0 100-101 17.79728024693531 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20371376162604093 1101 2 0.16337908420653235 1101 3 0.23649104311700597 1101 4 0.09458495813964873 1101 5 0.11229652937194601 1101 6 0.10981782818883801 1101 7 0.14410640263835717 1101 8 0.24089826327725206 1101 9 0.20025548801782378 1101 10-11 0.20888538743728446 1101 12-13 0.24835348872427687 1101 14-15 0.3785206549621165 1101 16-17 0.2930134437828258 1101 18-19 0.2737264352400288 1101 20-21 0.23891994997384103 1101 22-23 0.32086613483387794 1101 24-25 0.2730913458509505 1101 26-27 0.3222760214268092 1101 28-29 0.2751275926997039 1101 30-31 0.20076284302653136 1101 32-33 0.17065325804739473 1101 34-35 0.1744760440405102 1101 36-37 0.26344837145435207 1101 38-39 0.252125498451214 1101 40-41 0.21441885842036612 1101 42-43 0.2678554853488677 1101 44-45 0.24527723831685933 1101 46-47 0.2761533939406391 1101 48-49 0.22864574323166664 1101 50-51 0.28804988345221716 1101 52-53 0.19708893718986786 1101 54-55 0.1666017368565278 1101 56-57 0.23047933559638523 1101 58-59 0.11051624140605298 1101 60-61 0.23087339244382576 1101 62-63 0.1298388613810033 1101 64-65 0.004142909814689233 1101 66-67 -0.01868987837647751 1101 68-69 0.011971102027125369 1101 70-71 -0.05064575158232998 1101 72-73 -0.07399374270061188 1101 74-75 0.019891943282583924 1101 76-77 0.056241559256761775 1101 78-79 0.008706891257840965 1101 80-81 0.21879554009456825 1101 82-83 0.18501579474064656 1101 84-85 0.07229223414939057 1101 86-87 0.18092446774913995 1101 88-89 0.31791354266539784 1101 90-91 0.5457116369787443 1101 92-93 0.41743407448589664 1101 94-95 0.47155856319484357 1101 96-97 0.007975816558147386 1101 98-99 -0.2856965230337991 1101 100-101 -0.7185373499221086 1102 1 0.25263083475795156 1102 2 0.19694411907259735 1102 3 0.2012043746372001 1102 4 0.1480610353045293 1102 5 0.19780385853504612 1102 6 0.22472706729100622 1102 7 0.24110189905171353 1102 8 0.23560149746461434 1102 9 0.25136851079209777 1102 10-11 0.25708288706145055 1102 12-13 0.33079254167232364 1102 14-15 0.3281138486834081 1102 16-17 0.2573077345695367 1102 18-19 0.2135104665625036 1102 20-21 0.3454715360337772 1102 22-23 0.25622075356080387 1102 24-25 0.2847551436019202 1102 26-27 0.23311640200503092 1102 28-29 0.3023265902985699 1102 30-31 0.18979858326504484 1102 32-33 0.20732138261772803 1102 34-35 0.2448657931304581 1102 36-37 0.2716433247204364 1102 38-39 0.1660931250323472 1102 40-41 0.2619688271450684 1102 42-43 0.20342765285943898 1102 44-45 0.23145841453535354 1102 46-47 0.21033032206087654 1102 48-49 0.24226932728180373 1102 50-51 0.09849100304266045 1102 52-53 0.20879152711453486 1102 54-55 0.18547235316328425 1102 56-57 0.1954772851529185 1102 58-59 0.15625434563678908 1102 60-61 0.1832564965623007 1102 62-63 -0.028736724103737288 1102 64-65 0.08642764664318037 1102 66-67 0.16876029925757052 1102 68-69 0.27686282070798995 1102 70-71 0.2638340169491755 1102 72-73 0.2143244702107303 1102 74-75 0.0972691148983067 1102 76-77 0.27804568805132845 1102 78-79 0.46207415140213826 1102 80-81 0.5259695913417275 1102 82-83 0.34541859107377704 1102 84-85 0.2743855337321115 1102 86-87 0.5121233789537136 1102 88-89 0.6790242038062395 1102 90-91 0.6295800323269773 1102 92-93 0.46228109548852103 1102 94-95 0.5379225940682986 1102 96-97 0.32447306231381745 1102 98-99 0.5028693629865515 1102 100-101 -0.10278590857260639 1103 1 0.15456813577021933 1103 2 0.17845252742437623 1103 3 0.1655418026928288 1103 4 0.10780830503016148 1103 5 0.15330614851372815 1103 6 0.20617233994348538 1103 7 0.21946427480672526 1103 8 0.2025550593657215 1103 9 0.2333839346504476 1103 10-11 0.24093503593597632 1103 12-13 0.2295416277210478 1103 14-15 0.14315450917655426 1103 16-17 0.21342787215573367 1103 18-19 0.24982893295501896 1103 20-21 0.265608736249483 1103 22-23 0.391539304869454 1103 24-25 0.3264965254411081 1103 26-27 0.2900688004447787 1103 28-29 0.2950615736517008 1103 30-31 0.3011343912972322 1103 32-33 0.20305627664350112 1103 34-35 0.22703602351786145 1103 36-37 0.1907123185303803 1103 38-39 0.3238056883196805 1103 40-41 0.35238397331661986 1103 42-43 0.3090744932623153 1103 44-45 0.27020002355278194 1103 46-47 0.26695642748700976 1103 48-49 0.25207562475075207 1103 50-51 0.25040280164363793 1103 52-53 0.28821637467903827 1103 54-55 0.21652588120820582 1103 56-57 0.1657705601257078 1103 58-59 0.11281229759190126 1103 60-61 0.16776412633235793 1103 62-63 0.12573220816958042 1103 64-65 0.16205304847846946 1103 66-67 0.009905917198921088 1103 68-69 -0.10425665519402116 1103 70-71 -0.014544239226268019 1103 72-73 -0.1788019544780326 1103 74-75 -0.19276677595467717 1103 76-77 -0.05911976166784427 1103 78-79 -0.10402878790929293 1103 80-81 0.19575003350115594 1103 82-83 0.15627644256317197 1103 84-85 -1.792159251898795E-4 1103 86-87 -0.12392647122996081 1103 88-89 0.003389097269572261 1103 90-91 0.3130463090410984 1103 92-93 0.14931026583580476 1103 94-95 0.07964564174153921 1103 96-97 -0.07069617444450316 1103 98-99 -0.2560567566657994 1103 100-101 -0.3803405057889009 1104 1 -0.003472369682434362 1104 2 0.027350455991687284 1104 3 0.1250652000732444 1104 4 0.05508486148968217 1104 5 0.09164400168627651 1104 6 0.022561084582186197 1104 7 0.039459274133555766 1104 8 0.027853657253899655 1104 9 0.028404418177075286 1104 10-11 0.13039611723538513 1104 12-13 0.05404204123824741 1104 14-15 -0.05651900456567205 1104 16-17 0.11207769040049698 1104 18-19 0.1343662745202252 1104 20-21 0.1142518228188365 1104 22-23 0.12173375412223919 1104 24-25 0.0715030647829451 1104 26-27 0.16245787205057383 1104 28-29 0.13642002575101486 1104 30-31 0.08834613133915781 1104 32-33 0.1474766793900173 1104 34-35 0.04225393746781947 1104 36-37 0.019903267311953243 1104 38-39 -0.021699956670993004 1104 40-41 0.10205460205510519 1104 42-43 0.042988193183461476 1104 44-45 0.053473733475271956 1104 46-47 0.021062749491896682 1104 48-49 -0.01950179913323069 1104 50-51 -0.07697059477511203 1104 52-53 -0.08062895065321385 1104 54-55 -0.08191092759297902 1104 56-57 -0.07367273031723443 1104 58-59 -0.14891043431432394 1104 60-61 -0.25333342526112546 1104 62-63 -0.19960245226548068 1104 64-65 -0.1285942501963504 1104 66-67 -0.13047836530064671 1104 68-69 -0.2933085467948118 1104 70-71 -0.4910362669992381 1104 72-73 -0.6334400788409269 1104 74-75 -0.8420200408172569 1104 76-77 -0.7119071561247914 1104 78-79 -0.512557339829744 1104 80-81 -0.4633338816412689 1104 82-83 -0.5083659131385581 1104 84-85 -0.6866995167349152 1104 86-87 -0.6899714195883924 1104 88-89 -0.46516676601971696 1104 90-91 -0.29154469359387036 1104 92-93 -0.5254536446906144 1104 94-95 -0.24836966801169424 1104 96-97 -0.815322854959188 1104 98-99 -1.0215876935226795 1104 100-101 -1.1067195375816432 1105 1 0.12009435397170876 1105 2 0.1439211016034676 1105 3 0.11987923645533272 1105 4 0.12635666384905875 1105 5 0.020994256631603037 1105 6 0.10207307959333178 1105 7 0.1570753790111752 1105 8 0.1614006807045243 1105 9 0.1619000340680543 1105 10-11 0.15757327905243557 1105 12-13 0.11636595742997713 1105 14-15 0.1588303627596801 1105 16-17 0.17327761517496043 1105 18-19 0.21573953318601724 1105 20-21 0.21874414604270243 1105 22-23 0.18092000086922155 1105 24-25 0.20726358408712997 1105 26-27 0.17496657129465376 1105 28-29 0.18607585343081467 1105 30-31 0.19750026773607487 1105 32-33 0.21380728528107085 1105 34-35 0.21859090859640418 1105 36-37 0.27798045408362526 1105 38-39 0.19566528448213205 1105 40-41 0.27539081834504486 1105 42-43 0.23241471731427765 1105 44-45 0.21050897426156467 1105 46-47 0.27705741343887524 1105 48-49 0.19974162625321412 1105 50-51 0.10526352093477698 1105 52-53 0.11713037723556141 1105 54-55 0.15955167172824503 1105 56-57 0.2360977284706891 1105 58-59 0.14250481321953856 1105 60-61 -0.07576021857121873 1105 62-63 0.02779966283269175 1105 64-65 0.003277635965091008 1105 66-67 -0.11693802741606873 1105 68-69 -0.2353860476950942 1105 70-71 -0.1349054892674424 1105 72-73 -0.3357541423476036 1105 74-75 -0.3117505463731476 1105 76-77 -0.45973150553499664 1105 78-79 -0.6436718040570568 1105 80-81 -0.23434769038344427 1105 82-83 -0.19495442998137946 1105 84-85 -0.1720779409562958 1105 86-87 -0.23296996829357042 1105 88-89 -0.2432876053010844 1105 90-91 -0.2336910392913687 1105 92-93 -0.10684356560563657 1105 94-95 -0.24831696880481857 1105 96-97 -0.5480031572873081 1105 98-99 -0.2511118558507057 1105 100-101 -0.8993857454552163 1106 1 -0.004264480361300116 1106 2 0.08700929670607138 1106 3 0.0816843603448163 1106 4 0.04665305842307532 1106 5 0.15170623678472595 1106 6 0.0877237026269242 1106 7 0.11199434211335557 1106 8 0.10884988447881483 1106 9 0.17032534477954187 1106 10-11 0.1371338663453301 1106 12-13 0.16391766214672998 1106 14-15 0.0548145555188313 1106 16-17 0.036058347715702155 1106 18-19 -0.02819710698761213 1106 20-21 0.1179755022210216 1106 22-23 0.14774585899060355 1106 24-25 0.18786969105956075 1106 26-27 0.19039154125908198 1106 28-29 0.21572801964088484 1106 30-31 0.1789066521380107 1106 32-33 0.096165622334766 1106 34-35 0.0041103918285756436 1106 36-37 0.0702637915525628 1106 38-39 0.14672580307197336 1106 40-41 0.13343053626486778 1106 42-43 0.10556847980098638 1106 44-45 0.16005437641607756 1106 46-47 0.12644004218413585 1106 48-49 0.04520427972150998 1106 50-51 0.14082264054908222 1106 52-53 0.20558645081276694 1106 54-55 0.06987190349663308 1106 56-57 -0.02518442166839918 1106 58-59 -0.04797883117340973 1106 60-61 -0.08371165431422156 1106 62-63 -0.04585832476653451 1106 64-65 0.03327394663581629 1106 66-67 0.028553164247355767 1106 68-69 -0.40450215889037366 1106 70-71 -0.45126497187550285 1106 72-73 -0.33462783994641754 1106 74-75 -0.3244146850355598 1106 76-77 -0.28439009158067563 1106 78-79 -0.17394217199844775 1106 80-81 0.022021687478869012 1106 82-83 -0.13573503266578157 1106 84-85 -0.19445815225973817 1106 86-87 -0.27099421921628064 1106 88-89 -0.40716756167338275 1106 90-91 0.0802867371554079 1106 92-93 0.1406210900661904 1106 94-95 -0.0959860475445673 1106 96-97 -0.3776171719067136 1106 98-99 -0.4182547043652356 1106 100-101 -1.1320369639153505 1107 1 -0.057306086137231205 1107 2 -0.10038204379492299 1107 3 -0.02599355928036573 1107 4 0.031191032737623914 1107 5 -0.004010292266492854 1107 6 -0.07365243916398612 1107 7 -0.055362552310946 1107 8 -0.05226533114846177 1107 9 -0.002837395728910508 1107 10-11 0.009496767806048467 1107 12-13 0.0033792558225655966 1107 14-15 -0.04245045084184085 1107 16-17 -0.08563407863955774 1107 18-19 -0.044751103888991395 1107 20-21 0.03329325389768911 1107 22-23 0.0344924346931208 1107 24-25 -0.01926434565934443 1107 26-27 -0.06994037020188415 1107 28-29 -0.015184227927385052 1107 30-31 -0.031266744289119686 1107 32-33 -0.07408893837739328 1107 34-35 -0.16977730287442938 1107 36-37 -0.0711104004604195 1107 38-39 -0.07929694624871075 1107 40-41 0.07797608030547565 1107 42-43 -0.09454922370468921 1107 44-45 -0.20648595292436056 1107 46-47 -0.23298455349978298 1107 48-49 -0.3589101340118077 1107 50-51 -0.43539838398275776 1107 52-53 -0.252088314759888 1107 54-55 -0.4651000875611473 1107 56-57 -0.4077769668263116 1107 58-59 -0.35358167096761406 1107 60-61 -0.39635448271560847 1107 62-63 -0.5280403174204622 1107 64-65 -0.44420564043180377 1107 66-67 -0.48503912954211614 1107 68-69 -0.5947957056033246 1107 70-71 -0.5900633543244425 1107 72-73 -0.5092478960885565 1107 74-75 -0.6661201959299419 1107 76-77 -0.6730176295177159 1107 78-79 -0.6466856624641295 1107 80-81 -0.5293671469840326 1107 82-83 -0.7356229204350342 1107 84-85 -0.7272581480543359 1107 86-87 -0.9401460562607191 1107 88-89 -1.176797715562472 1107 90-91 -0.9088843491671845 1107 92-93 -0.8508123121146909 1107 94-95 -0.8699874315045868 1107 96-97 -1.0040347034687436 1107 98-99 -0.7875880427960276 1107 100-101 -1.256204604674501 1108 1 -0.02872642100116707 1108 2 0.042242473537463354 1108 3 0.023531525653055496 1108 4 -0.010249814781907673 1108 5 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-0.31078550312192377 2316 100-101 0.6786891519658163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 11.0 5 29.0 6 84.0 7 211.0 8 529.0 9 1121.0 10 1933.0 11 3092.0 12 4803.0 13 6763.0 14 8852.0 15 12035.0 16 15476.0 17 21940.0 18 22348.0 19 16362.0 20 14859.0 21 15041.0 22 15847.0 23 17150.0 24 19371.0 25 22105.0 26 25776.0 27 30459.0 28 36340.0 29 44062.0 30 53256.0 31 65883.0 32 82490.0 33 106880.0 34 148473.0 35 221547.0 36 347882.0 37 484078.0 38 279905.0 39 11947.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37749776635283 19.16187884028016 15.109640565358074 22.350982828008938 2 15.445824027094703 21.10224155675964 39.52789926195467 23.92403515419098 3 20.459011017413125 23.93130722120887 30.18459007311901 25.425091688258995 4 12.96442801255798 16.947806939126778 37.41941669685115 32.6683483514641 5 13.347485300697656 37.78450225248802 34.33800567499141 14.530006771822917 6 31.377625934136375 36.70842160943349 16.87189026497189 15.042062191458239 7 27.931083037741566 33.227278496080956 20.62288727925571 18.218751186921768 8 25.885038347415822 34.387798490559746 19.501506753758548 20.225656408265884 9 25.39943101865447 17.94672029777493 20.927156121551903 35.72669256201869 10-11 25.108270424549755 26.35901963272819 26.878022887093977 21.65468705562807 12-13 26.771744175166955 23.84302340123375 27.310988788047503 22.074243635551795 14-15 22.620453745391213 25.572740073740576 26.884159672428808 24.922646508439406 16-17 21.49219357768822 28.57176273701066 27.593855283870848 22.342188401430267 18-19 21.309703901811346 27.575307580643514 29.661232842322132 21.453755675223 20-21 22.352041227524914 25.843629252422247 29.780990353626258 22.02333916642658 22-23 21.769187371986146 25.921329204157956 30.070830859132187 22.23865256472371 24-25 22.439460579000524 26.084343529905468 29.890532155875242 21.58566373521876 26-27 21.727831267595977 25.8453480842328 30.632056340481196 21.794764307690027 28-29 21.218632530850144 27.65872397368251 29.7547482114524 21.367895284014942 30-31 21.736856781040377 26.259387682693315 29.52125713195235 22.482498404313958 32-33 21.541401008362882 26.390227634330653 29.817503456549378 22.250867900757083 34-35 22.674443383089372 26.303814485427573 29.456432815451766 21.56530931603129 36-37 22.392433169976847 25.781452880475637 30.126959380179695 21.69915456936782 38-39 21.731164896828005 26.625458713364896 29.85179869380486 21.791577696002236 40-41 22.058686263187894 26.727336021462417 29.437121149479754 21.77685656586993 42-43 21.685798481205456 26.88264489914657 28.713819075516568 22.71773754413141 44-45 22.152115679732375 26.31434133895853 29.105285806331775 22.428257174977315 46-47 22.96518380841541 27.181063882760974 28.427892326309877 21.425859982513735 48-49 22.501229233025757 27.083263369921205 28.3874894533775 22.028017943675536 50-51 21.727092461123085 27.45039322738265 29.034141465513684 21.78837284598058 52-53 22.60658673260007 27.64043195151708 27.79645716011424 21.9565241557686 54-55 21.83102310108729 27.07856055686432 28.38046898810809 22.7099473539403 56-57 22.47208706104139 27.033180558096664 28.078969126124104 22.415763254737847 58-59 23.104451175197887 26.707815460836077 27.88407870071652 22.303654663249514 60-61 21.947168769557184 27.223505930236946 29.046986338140346 21.78233896206552 62-63 21.675261665548433 27.30812602004576 28.908748185716824 22.107864128688984 64-65 21.448644602519725 28.078009641078943 28.18751050888722 22.285835247514115 66-67 21.797112082751728 27.26602981205864 27.865064084971387 23.07179402021825 68-69 21.80710055608439 27.13335139854876 28.284754116205658 22.774793929161195 70-71 22.499165680266444 27.09548578020417 27.74260755660926 22.662740982920127 72-73 22.15182779004199 26.79823858493913 28.60106274080037 22.448870884218504 74-75 21.61441277364047 28.376693997904535 27.610162551541357 22.39873067691364 76-77 21.93125100982483 27.105425364296114 27.90575138269214 23.057572243186915 78-79 22.0324213440515 27.447282423892027 27.97589431560963 22.544401916446848 80-81 22.622969575094427 27.106237625573865 27.687217584011776 22.583575215319936 82-83 22.22251042869993 27.109154189127473 28.344641320347986 22.32369406182461 84-85 21.83834148700338 27.734065604234658 28.037361749668587 22.39023115909337 86-87 21.99614629429171 27.73657271510515 27.587126276676223 22.68015471392692 88-89 21.669505883283197 27.807632511956687 27.018799296261886 23.50406230849823 90-91 21.963106970435433 27.10682251724201 27.414985415173337 23.51508509714923 92-93 22.42422923037445 27.337020008837648 27.607383417649167 22.631367343138738 94-95 21.906870296894873 26.93842273035083 27.96251041017237 23.192196562581927 96-97 21.28886039761995 26.760071812819774 28.687748558787483 23.263319230772794 98-99 21.554223165173152 27.37517216175412 27.348955635013144 23.72164903805959 100-101 21.727001887038906 25.71340631365977 28.488011235153582 24.071580564147744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 458.0 1 330.0 2 280.0 3 388.5 4 349.0 5 325.5 6 606.5 7 1289.5 8 2096.5 9 2532.5 10 2331.0 11 1984.0 12 2021.5 13 2242.0 14 2540.0 15 2873.0 16 3139.5 17 3413.0 18 3740.0 19 4176.0 20 4745.5 21 5542.5 22 6674.5 23 8148.0 24 9893.0 25 12310.5 26 15423.5 27 18711.0 28 21945.0 29 25050.0 30 28225.5 31 32756.0 32 39118.0 33 41619.5 34 42054.0 35 45096.0 36 49397.5 37 53006.5 38 55077.0 39 56919.5 40 59064.0 41 61719.0 42 63063.0 43 64266.5 44 65947.5 45 69413.0 46 72806.5 47 85587.0 48 109555.0 49 106417.5 50 83038.5 51 72494.0 52 69119.0 53 68267.0 54 68641.0 55 64346.5 56 57987.0 57 52732.5 58 49204.0 59 44145.5 60 35660.5 61 29674.5 62 25293.0 63 19121.5 64 12900.0 65 9295.5 66 7080.0 67 5593.0 68 4461.5 69 3501.5 70 2722.0 71 1944.0 72 1434.0 73 1177.5 74 858.5 75 577.0 76 443.0 77 286.5 78 190.0 79 126.5 80 69.5 81 42.0 82 28.5 83 20.0 84 13.0 85 7.0 86 4.5 87 3.0 88 2.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41108022155255386 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012042913532807212 16-17 0.0023391043592567856 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.9478347304657E-5 26-27 0.0033349606706235355 28-29 6.9478347304657E-5 30-31 0.0 32-33 4.6318898203104666E-5 34-35 0.012969291496869305 36-37 0.02679548261049605 38-39 0.02144564986803746 40-41 0.009055344598706962 42-43 0.009009025700503857 44-45 0.004122381940076315 46-47 0.005025600455036856 48-49 0.0036823524071468204 50-51 7.874212694527794E-4 52-53 0.0 54-55 0.0 56-57 4.6318898203104666E-5 58-59 0.0015285236407024537 60-61 0.002431742155662995 62-63 0.0016443208862102156 64-65 0.002732814993983175 66-67 0.002524379952069204 68-69 0.004678208718513571 70-71 6.9478347304657E-5 72-73 0.0 74-75 0.0 76-77 0.09321678263374814 78-79 0.017045354538742515 80-81 3.4739173652328497E-4 82-83 0.0 84-85 0.0 86-87 0.10748300328030436 88-89 0.4168006054806373 90-91 0.42191884373208044 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1579724550776165E-4 100-101 3.7055118562483733E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2158946.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.23415262683735 #Duplication Level Percentage of deduplicated Percentage of total 1 74.5818243634545 38.21136572625204 2 14.110510799438932 14.45880127882182 3 4.867161138966345 7.480946299596397 4 2.1663082036064236 4.439558605613654 5 1.1652023680243573 2.984907798225611 6 0.6914527714338556 2.1255598095535104 7 0.4711248722792042 1.68963785288564 8 0.3186776102981886 1.306174185981855 9 0.23804486298788577 1.0976424158120333 >10 1.256545033592391 11.65526319242249 >50 0.08458774588263986 2.96594561428706 >100 0.04042471916112928 3.8086335706424737 >500 0.0038869663065067245 1.40809709343939 >1k 0.003706177175971518 3.835124069550789 >5k 4.5197282633799E-4 1.7110297038915323 >10k+ 9.0394565267598E-5 0.8213127830237498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17734 0.8214193407338581 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8733 0.404502938007713 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8424 0.39019039846295367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7554 0.34989295702625267 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6498 0.3009802005237741 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5736 0.26568520009300833 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4858 0.22501720747068243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4653 0.215521833339046 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4392 0.20343260090803567 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3962 0.18351547468070067 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3944 0.18268173451304479 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3821 0.17698451003406293 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3548 0.16433945082461535 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2874 0.13312051343572281 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2762 0.12793279683697506 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2241 0.10380065087315755 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2208 0.1022721272324551 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2164 0.10023409571151848 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3159449101552333E-5 0.0 12-13 4.6318898203104666E-5 0.0 0.0 6.9478347304657E-5 0.0 14-15 4.6318898203104666E-5 0.0 0.0 9.263779640620933E-5 0.0 16-17 4.6318898203104666E-5 0.0 0.0 9.263779640620933E-5 0.0 18-19 9.263779640620933E-5 0.0 0.0 1.1579724550776167E-4 0.0 20-21 9.263779640620933E-5 0.0 0.0 1.6211614371086633E-4 0.0 22-23 9.263779640620933E-5 0.0 0.0 3.2423228742173266E-4 0.0 24-25 1.38956694609314E-4 0.0 0.0 6.716240239450176E-4 0.0 26-27 1.38956694609314E-4 0.0 0.0 0.0010653346586714072 0.0 28-29 1.38956694609314E-4 0.0 0.0 0.003010728383201803 0.0 30-31 1.38956694609314E-4 0.0 0.0 0.008777431209488333 0.0 32-33 1.38956694609314E-4 0.0 0.0 0.019708691185421033 0.0 34-35 1.6211614371086633E-4 0.0 0.0 0.035711870514593694 0.0 36-37 1.8527559281241866E-4 0.0 0.0 0.061488337364621434 0.0 38-39 1.8527559281241866E-4 0.0 0.0 0.10069728469354955 0.0 40-41 1.8527559281241866E-4 0.0 0.0 0.14062417494462576 0.0 42-43 2.0843504191397097E-4 0.0 0.0 0.18645672471659783 0.0 44-45 2.315944910155233E-4 0.0 0.0 0.2456985955183687 0.0 46-47 2.5475394011707564E-4 0.0 0.0 0.3172612932421654 0.0 48-49 2.77913389218628E-4 0.0 0.0 0.39178840045096075 0.0 50-51 2.77913389218628E-4 0.0 0.0 0.47682989755186095 0.0 52-53 2.77913389218628E-4 0.0 0.0 0.5636315127844791 0.0 54-55 2.77913389218628E-4 0.0 0.0 0.6597432265559213 0.0 56-57 2.77913389218628E-4 0.0 0.0 0.7862632969977017 0.0 58-59 2.77913389218628E-4 0.0 0.0 0.9146361233676062 0.0 60-61 2.77913389218628E-4 0.0 0.0 1.05463499318649 0.0 62-63 2.77913389218628E-4 0.0 0.0 1.1969729673646308 0.0 64-65 2.77913389218628E-4 0.0 0.0 1.353507683842023 0.0 66-67 2.77913389218628E-4 0.0 0.0 1.5406823514807688 0.0 68-69 2.77913389218628E-4 0.0 0.0 1.758010621849736 0.0 70-71 2.77913389218628E-4 0.0 0.0 1.9866638628293622 0.0 72-73 3.010728383201803E-4 0.0 0.0 2.222681808623282 0.0 74-75 3.2423228742173266E-4 0.0 0.0 2.4759767034469595 0.0 76-77 3.2423228742173266E-4 0.0 0.0 2.7503235375039488 0.0 78-79 3.2423228742173266E-4 0.0 0.0 3.0894705101470814 0.0 80-81 3.2423228742173266E-4 0.0 0.0 3.4505957999875863 0.0 82-83 3.2423228742173266E-4 0.0 0.0 3.805190125181454 0.0 84-85 3.7055118562483733E-4 0.0 0.0 4.1724063501356685 0.0 86-87 3.7055118562483733E-4 0.0 0.0 4.557686945389093 0.0 88-89 3.9371063472638964E-4 0.0 0.0 4.987433682917498 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 510 0.0 30.24186 94-95 GTACCGA 125 2.2708502E-4 22.7791 6 TACATGG 7980 0.0 22.538843 2 GTACATG 8335 0.0 22.356354 1 GAGTACT 9745 0.0 22.109524 12-13 ACATGGG 8045 0.0 21.589888 3 GTACTTT 10040 0.0 21.436258 14-15 AGTACTT 9925 0.0 20.991302 12-13 AGAGTAC 13895 0.0 20.338953 10-11 CATGGGG 4615 0.0 18.818054 4 GTAAGGT 1170 0.0 18.658094 4 AGGTAAG 1070 0.0 18.627768 2 TACTTTT 11190 0.0 18.469852 14-15 TAAGGTG 1405 0.0 18.239492 5 ACTTTTT 11885 0.0 18.128088 16-17 GGTAAGG 1210 0.0 17.256895 3 GTGTACG 255 5.3399763E-6 16.820238 1 CGCCGAT 85 0.0017996448 16.74934 48-49 CTAGACT 1330 0.0 16.413513 4 GTGTAGC 1310 0.0 16.007046 1 >>END_MODULE