FastQCFastQC Report
Fri 27 May 2016
SRR939217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203641
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.2784936384828563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51310.23284191935074727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.14571338979443565No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27330.12402201629031226No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26060.11825882709570207No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25910.11757813545854338No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25470.11558143998954458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.045.0792921
GTATCAA67300.037.7388841
ATCAACG85150.029.2645973
TATCAAC86250.029.1110762
TCAACGC85600.029.1107544
CAACGCA87550.028.4623745
AACGCAG90550.027.5081376
TAAGGTG13100.026.399345
GTACATG77800.024.8960761
TACATGG77000.024.2408032
ACGCAGA102500.024.2086897
CGCAGAG104900.023.5650718
ACATGGG75350.022.6968423
GTAAGGT13950.022.073984
AGGTAAG13500.021.4061012
GCAGAGT117750.020.5912539
GAGTACT68100.020.54915812-13
GGTAAGG15250.020.1922633
GTACTTT69800.020.01566114-15
AGAGTAC100150.019.93060110-11
Produced by FastQC (version 0.11.5)