FastQCFastQC Report
Fri 27 May 2016
SRR939217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203641
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61520.279174330120015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.24273463781078677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.15333713613061292No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27140.1231598068832446No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26210.11893951873286075No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25920.11762351490102063No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24290.1102266657772296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.044.474621
GTATCAA76200.036.4354861
ATCAACG94450.028.8370763
TATCAAC96700.028.2642422
TCAACGC96500.028.2244784
CAACGCA97850.027.8350735
AACGCAG100400.027.1281096
ACGCAGA114050.023.8812987
TAAGGTA8250.023.581494
CGCAGAG115800.023.5613738
GTGTAAG7900.021.7013321
GAGTACT74300.021.01203512-13
TACATGG74050.020.953862
GCAGAGT129800.020.8377749
GTACATG76250.020.79771
TAAGGTG13800.020.6306785
AGAGTAC108800.020.30260810-11
TGTAAGG8900.020.2597892
GTACTTT77100.019.91044614-15
CAGAGTA120500.019.63085210-11
Produced by FastQC (version 0.11.5)