##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939217_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203641 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.508446702525504 34.0 31.0 34.0 31.0 34.0 2 32.72220747390342 34.0 31.0 34.0 31.0 34.0 3 32.810661536974486 34.0 31.0 34.0 31.0 34.0 4 36.268710284479184 37.0 37.0 37.0 35.0 37.0 5 36.22562522661359 37.0 37.0 37.0 35.0 37.0 6 36.25959627725206 37.0 37.0 37.0 35.0 37.0 7 36.21200776351502 37.0 37.0 37.0 35.0 37.0 8 36.194992741558174 37.0 37.0 37.0 35.0 37.0 9 37.945308242132 39.0 38.0 39.0 35.0 39.0 10-11 37.886091926951806 39.0 38.0 39.0 35.0 39.0 12-13 37.816453315217856 39.0 38.0 39.0 35.0 39.0 14-15 39.19920758417546 40.5 39.0 41.0 36.0 41.0 16-17 39.13208594321852 40.0 38.5 41.0 36.0 41.0 18-19 39.14548240843223 40.5 38.5 41.0 36.0 41.0 20-21 39.202541384917055 41.0 39.0 41.0 36.0 41.0 22-23 39.16187005959682 41.0 39.0 41.0 36.0 41.0 24-25 39.100933409752315 41.0 39.0 41.0 35.0 41.0 26-27 38.96209092134336 40.0 38.5 41.0 35.0 41.0 28-29 38.808997019024424 40.0 38.0 41.0 35.0 41.0 30-31 38.60450749464182 40.0 38.0 41.0 34.5 41.0 32-33 38.46554565829915 40.0 38.0 41.0 34.0 41.0 34-35 38.424608636343216 40.0 38.0 41.0 34.0 41.0 36-37 38.31198820497531 40.0 38.0 41.0 34.0 41.0 38-39 38.164618238633246 40.0 38.0 41.0 33.5 41.0 40-41 37.975626020753836 40.0 38.0 41.0 33.0 41.0 42-43 37.80437557660254 40.0 37.0 41.0 33.0 41.0 44-45 37.58465784581064 40.0 37.0 41.0 33.0 41.0 46-47 37.300282804685516 40.0 37.0 41.0 32.0 41.0 48-49 36.96398755514169 40.0 36.0 41.0 31.0 41.0 50-51 36.68191098277805 39.5 36.0 41.0 31.0 41.0 52-53 36.41435673959597 39.0 35.0 41.0 30.5 41.0 54-55 36.1330089610785 39.0 35.0 41.0 30.0 41.0 56-57 35.820019458704934 38.5 35.0 40.0 29.0 41.0 58-59 35.47834719902198 38.0 35.0 40.0 28.5 41.0 60-61 35.08704344310166 37.5 34.5 40.0 28.0 41.0 62-63 34.65891789996647 37.0 34.0 40.0 27.5 41.0 64-65 34.22712592477632 36.5 34.0 39.0 26.0 41.0 66-67 33.844967034104016 36.0 33.5 39.0 26.0 40.5 68-69 33.3654048005097 35.0 33.0 38.5 26.0 40.0 70-71 32.911449732510874 35.0 33.0 38.0 25.0 40.0 72-73 32.45074106898537 35.0 32.0 37.0 24.0 39.0 74-75 31.97325970972586 35.0 32.0 36.5 22.5 39.0 76-77 30.04550990837437 33.5 29.5 35.0 20.0 37.0 78-79 31.0205185418133 34.0 31.0 35.0 20.0 37.0 80-81 31.078111407438872 35.0 31.0 35.0 20.0 37.0 82-83 30.811396457045408 34.0 31.0 35.0 19.5 37.0 84-85 30.461928236042077 34.0 31.0 35.0 17.5 36.0 86-87 29.93221400400519 34.0 30.5 35.0 10.0 36.0 88-89 29.389280967271894 34.0 30.0 35.0 4.5 35.0 90-91 28.95115061845373 34.0 29.0 35.0 2.0 35.0 92-93 28.670424311400993 34.0 29.0 35.0 2.0 35.0 94-95 28.344837022001315 34.0 29.0 35.0 2.0 35.0 96-97 27.789247885658327 34.0 28.5 35.0 2.0 35.0 98-99 27.003812553859724 33.0 27.0 35.0 2.0 35.0 100-101 23.360722776532114 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20993240563353055 1101 2 0.23680662710903277 1101 3 0.21317738330821356 1101 4 0.17231394309982306 1101 5 0.21448770762377478 1101 6 0.2177623087861491 1101 7 0.2047490768932505 1101 8 0.2391083608323541 1101 9 0.28549917946461534 1101 10-11 0.32362922645303627 1101 12-13 0.3323957574594729 1101 14-15 0.4051569625410991 1101 16-17 0.34669891186612034 1101 18-19 0.3749811377236014 1101 20-21 0.35223106641856816 1101 22-23 0.3596354276897529 1101 24-25 0.34429353760286574 1101 26-27 0.37161580463118327 1101 28-29 0.30595526294086284 1101 30-31 0.3897289058555131 1101 32-33 0.3606706736501337 1101 34-35 0.4553728589513426 1101 36-37 0.5179747905240788 1101 38-39 0.5631941264183027 1101 40-41 0.5527509676930933 1101 42-43 0.6322189379937839 1101 44-45 0.6459302357462562 1101 46-47 0.6561831852432221 1101 48-49 0.5454506734082756 1101 50-51 0.5736815736181171 1101 52-53 0.6033641905057436 1101 54-55 0.4470035451257601 1101 56-57 0.45950197382835967 1101 58-59 0.5095399005863399 1101 60-61 0.5645224430726117 1101 62-63 0.405226324254393 1101 64-65 0.3613691578618443 1101 66-67 0.5016070115775833 1101 68-69 0.47410644886036124 1101 70-71 0.5059635405478033 1101 72-73 0.3539792106208992 1101 74-75 0.3388298557640965 1101 76-77 0.31783690265643827 1101 78-79 0.5937110214311296 1101 80-81 0.5230280999041881 1101 82-83 0.6133529324611757 1101 84-85 0.6553391779607942 1101 86-87 0.7082455947612374 1101 88-89 0.9266003221909465 1101 90-91 0.9360511586661353 1101 92-93 0.7448122978519542 1101 94-95 0.565982576920085 1101 96-97 0.6477534091537969 1101 98-99 0.4892758060609417 1101 100-101 0.05832701476975899 1102 1 0.17826892225773605 1102 2 0.2124927128738534 1102 3 0.19410808656932232 1102 4 0.11908705837216615 1102 5 0.14172625198333577 1102 6 0.17521002086316884 1102 7 0.14818487827192683 1102 8 0.08783015225009905 1102 9 0.2075272940972681 1102 10-11 0.24463264282614716 1102 12-13 0.21858120100171874 1102 14-15 0.2516785840349556 1102 16-17 0.27508261295390923 1102 18-19 0.3741113527940456 1102 20-21 0.3269457387675274 1102 22-23 0.3367180037061246 1102 24-25 0.26285306264927755 1102 26-27 0.2800383889647904 1102 28-29 0.21245758971237194 1102 30-31 0.24962943013030525 1102 32-33 0.2483358065857999 1102 34-35 0.34864011195605826 1102 36-37 0.4395505849423387 1102 38-39 0.35996105851204874 1102 40-41 0.4392092456604928 1102 42-43 0.4472761931592686 1102 44-45 0.3989392268625096 1102 46-47 0.4551104905605712 1102 48-49 0.45873887176357897 1102 50-51 0.4461852321100679 1102 52-53 0.5698186437164381 1102 54-55 0.5682515375959056 1102 56-57 0.5693001351139699 1102 58-59 0.5981994702638147 1102 60-61 0.5898216595049561 1102 62-63 0.5761439373391184 1102 64-65 0.5492581939777494 1102 66-67 0.6428993199437869 1102 68-69 0.5166301579448813 1102 70-71 0.4872763188409195 1102 72-73 0.5482253021976433 1102 74-75 0.5796880328775771 1102 76-77 0.5801706078707944 1102 78-79 0.562261345159925 1102 80-81 0.46430419578913273 1102 82-83 0.4092331192866503 1102 84-85 0.46892702677286024 1102 86-87 0.5682720528896361 1102 88-89 0.6016080478255503 1102 90-91 0.6539688083424622 1102 92-93 0.5805357925150538 1102 94-95 0.5112995068904418 1102 96-97 0.3521349724376286 1102 98-99 0.35470513366497514 1102 100-101 0.05159108938029178 1103 1 0.04984406339415415 1103 2 0.05797128823810738 1103 3 0.09914099385283492 1103 4 0.0832936474111321 1103 5 0.13135186999551962 1103 6 0.12649525309691256 1103 7 0.12094661816593799 1103 8 0.11998382015768527 1103 9 0.1140543685446076 1103 10-11 0.2013470068880565 1103 12-13 0.2192509249944905 1103 14-15 0.21263133141464863 1103 16-17 0.1970559894719912 1103 18-19 0.1937528500146115 1103 20-21 0.2278274843892163 1103 22-23 0.24185465480950796 1103 24-25 0.1806701543539475 1103 26-27 0.1995039827752052 1103 28-29 0.21814748995691957 1103 30-31 0.24338738222217415 1103 32-33 0.36960131724747214 1103 34-35 0.4437685839479144 1103 36-37 0.4169727277670958 1103 38-39 0.3913215344733274 1103 40-41 0.40734388310914227 1103 42-43 0.4839916433293183 1103 44-45 0.419473330786154 1103 46-47 0.3415651851221071 1103 48-49 0.38773890685716594 1103 50-51 0.3782923305696215 1103 52-53 0.49252719248897137 1103 54-55 0.3682219257796646 1103 56-57 0.4348732479946733 1103 58-59 0.3128241725126415 1103 60-61 0.30133276636954776 1103 62-63 0.38954825364557166 1103 64-65 0.2826666754064533 1103 66-67 0.43242603551434655 1103 68-69 0.4076713539753598 1103 70-71 0.42940775995528213 1103 72-73 0.28508294544032964 1103 74-75 0.14190153663425775 1103 76-77 0.09651973733206987 1103 78-79 0.25444805227216705 1103 80-81 0.22994741899946547 1103 82-83 0.24388890881938963 1103 84-85 0.29532532055845095 1103 86-87 0.4181754942898728 1103 88-89 0.2663654012771559 1103 90-91 0.2905758526246771 1103 92-93 0.25504083843514636 1103 94-95 0.05661557588597432 1103 96-97 0.24761883745590652 1103 98-99 -0.08147192450354268 1103 100-101 -0.511274335417351 1104 1 0.03060954882936784 1104 2 0.05322469525629003 1104 3 0.14232448594142966 1104 4 0.06437227699151293 1104 5 0.08909174533103226 1104 6 0.08743157139174684 1104 7 0.1407441495915691 1104 8 0.08049305630346026 1104 9 0.11983876126245718 1104 10-11 0.12345295687397595 1104 12-13 0.08706986490145852 1104 14-15 0.13147756701174984 1104 16-17 0.14730116626125778 1104 18-19 0.17684949218007517 1104 20-21 0.21061456614282292 1104 22-23 0.11328748081802331 1104 24-25 0.18401204745488542 1104 26-27 0.16775346498753407 1104 28-29 0.11192291592688264 1104 30-31 0.11697724495282813 1104 32-33 0.1690135849844694 1104 34-35 0.25426447377654426 1104 36-37 0.21174196005727453 1104 38-39 0.38998826210465154 1104 40-41 0.3327812221500892 1104 42-43 0.2509974465057496 1104 44-45 0.2964842994816266 1104 46-47 0.2697408671436392 1104 48-49 0.21782286507824722 1104 50-51 0.3506521077355558 1104 52-53 0.3191324578582453 1104 54-55 0.41530130670826537 1104 56-57 0.5577191771207808 1104 58-59 0.3443162907708981 1104 60-61 0.4332726041376489 1104 62-63 0.49806975357389405 1104 64-65 0.49505687723242886 1104 66-67 0.5118437900706354 1104 68-69 0.3202343164623187 1104 70-71 0.22270296566158265 1104 72-73 0.20357216705345138 1104 74-75 0.16736284961528014 1104 76-77 0.20129784245161275 1104 78-79 0.2949708077429243 1104 80-81 0.1724121871937072 1104 82-83 0.18705126923915572 1104 84-85 0.14121416056576663 1104 86-87 0.26020749579257085 1104 88-89 0.5087416883317246 1104 90-91 0.4412744334662868 1104 92-93 0.42792198700931294 1104 94-95 0.3075640294432631 1104 96-97 0.3368292050727959 1104 98-99 0.032697909853833806 1104 100-101 -0.4177550845906204 1105 1 -0.012528347847997168 1105 2 0.016570093967288813 1105 3 0.03200882870726218 1105 4 0.040628913350339246 1105 5 0.08165830145188124 1105 6 0.073532160060239 1105 7 0.10486136345199526 1105 8 0.08579203539923697 1105 9 0.10278614691011256 1105 10-11 0.13894365032834344 1105 12-13 0.11244746950684004 1105 14-15 0.1100046443184155 1105 16-17 0.02686936372271731 1105 18-19 0.11158526522261525 1105 20-21 0.1348855895018417 1105 22-23 0.08318247833916814 1105 24-25 0.10037142403425037 1105 26-27 0.15212354638070025 1105 28-29 0.09373796908995047 1105 30-31 0.07826061927956829 1105 32-33 0.07978434120448696 1105 34-35 0.17366911477540015 1105 36-37 0.1179915689753841 1105 38-39 0.18574135345657083 1105 40-41 -0.009090531870235452 1105 42-43 0.03409296193964195 1105 44-45 0.15404842487811976 1105 46-47 0.010111709040657502 1105 48-49 -0.0859504133191038 1105 50-51 -0.02512199373876456 1105 52-53 -0.04934983154716832 1105 54-55 0.08486863777292797 1105 56-57 -0.07047337475471949 1105 58-59 -0.07302728850681461 1105 60-61 0.03333818556210133 1105 62-63 0.03409128353438007 1105 64-65 -0.11844507456702758 1105 66-67 -0.061357353573143314 1105 68-69 -0.2184155244576047 1105 70-71 -0.20515024746721622 1105 72-73 -0.02112242807451281 1105 74-75 -0.12219466228564357 1105 76-77 -0.21540809284779883 1105 78-79 0.07339403776645881 1105 80-81 0.0015753441511137112 1105 82-83 -0.019969071832385055 1105 84-85 -0.022540369967497753 1105 86-87 0.0890368493161553 1105 88-89 0.2683644687915887 1105 90-91 0.3531656092148765 1105 92-93 0.1605392995427053 1105 94-95 -0.206961955240768 1105 96-97 -0.33269324182429827 1105 98-99 -0.3569260258017657 1105 100-101 -0.8552338458357305 1106 1 0.025609879808023095 1106 2 0.062395168177509674 1106 3 0.05500900654249108 1106 4 -4.224689587246644E-4 1106 5 0.046789363230352876 1106 6 0.09928344425811275 1106 7 0.10319793078630823 1106 8 0.07705466088798829 1106 9 0.15462650937824662 1106 10-11 0.11257841669788604 1106 12-13 0.16510399352922178 1106 14-15 0.1055274218539708 1106 16-17 0.13132388085363544 1106 18-19 0.11476877121399554 1106 20-21 0.12534760821493762 1106 22-23 0.11574594299661811 1106 24-25 0.19900845397232558 1106 26-27 0.1886219056997973 1106 28-29 0.14782889907533558 1106 30-31 0.17486635764633007 1106 32-33 0.11452976984078589 1106 34-35 0.0309744513645569 1106 36-37 0.10897734262132275 1106 38-39 0.2191325877119965 1106 40-41 0.15849258984845704 1106 42-43 0.10064164444989387 1106 44-45 0.2227110482309982 1106 46-47 0.24628606464332847 1106 48-49 0.19073438975507173 1106 50-51 0.22078837637674553 1106 52-53 0.30674652728811225 1106 54-55 0.26086004596328394 1106 56-57 0.3244889199976555 1106 58-59 0.3777683035939603 1106 60-61 0.3180241810145361 1106 62-63 0.33483793612280977 1106 64-65 0.337489050249161 1106 66-67 0.42079818121175094 1106 68-69 0.3044724035948221 1106 70-71 0.30117091274281904 1106 72-73 0.2905379911430188 1106 74-75 0.21333247523114096 1106 76-77 0.15266927615659043 1106 78-79 0.319055415344085 1106 80-81 0.306928532811078 1106 82-83 0.18624817317023457 1106 84-85 0.28701829685332214 1106 86-87 0.44970317888523326 1106 88-89 0.695030188241887 1106 90-91 0.5547034196313803 1106 92-93 0.6313544720916333 1106 94-95 0.37907315026938804 1106 96-97 0.6241189154191886 1106 98-99 0.7762925271952206 1106 100-101 0.4588983902124504 1107 1 0.013312896884386305 1107 2 0.0019580400933634223 1107 3 0.04765110668640915 1107 4 0.008911109113064697 1107 5 0.009386443194600247 1107 6 0.06207375063557663 1107 7 0.02042077010336385 1107 8 0.06405405489037008 1107 9 0.060693053945612974 1107 10-11 0.034823005795956874 1107 12-13 0.002058578798745714 1107 14-15 -0.01885775149419544 1107 16-17 0.10973313484672076 1107 18-19 0.05132471172274933 1107 20-21 0.10048517237442667 1107 22-23 0.05421277801094959 1107 24-25 0.035802317155429364 1107 26-27 0.0868630054752586 1107 28-29 0.05654662700264623 1107 30-31 4.232838572377773E-4 1107 32-33 -0.020259487400480225 1107 34-35 -0.03008352060838604 1107 36-37 0.04404213307975624 1107 38-39 0.008159936785069988 1107 40-41 -0.06521129730911923 1107 42-43 0.01914463687244705 1107 44-45 -0.0768149734508512 1107 46-47 -0.2577175911374283 1107 48-49 -0.22342865644696275 1107 50-51 -0.23048797518465847 1107 52-53 -0.1802273356340578 1107 54-55 -0.237925259471659 1107 56-57 -0.2661504637293106 1107 58-59 -0.30165895442201673 1107 60-61 -0.2555729254930341 1107 62-63 -0.27960439985181296 1107 64-65 -0.36258047976751584 1107 66-67 -0.1819691404452186 1107 68-69 -0.11526339133954622 1107 70-71 -0.18584928852757088 1107 72-73 -0.23275287736809247 1107 74-75 -0.3320304057724357 1107 76-77 -0.2247871624219293 1107 78-79 -0.1927211609358146 1107 80-81 -0.30769465768766935 1107 82-83 -0.29981849286658857 1107 84-85 -0.24350832722705107 1107 86-87 -0.1892596789041754 1107 88-89 -0.2244630051946217 1107 90-91 -0.2842950017155772 1107 92-93 -0.47693138692911674 1107 94-95 -0.34143642759725523 1107 96-97 -0.31958370305739336 1107 98-99 -0.2661781435546189 1107 100-101 -0.5659398642084774 1108 1 -0.02447961490933892 1108 2 -0.027439589446288437 1108 3 0.006327053710897701 1108 4 0.05605969446422421 1108 5 -0.010506869352774117 1108 6 0.0546687519625948 1108 7 9.793609299606487E-4 1108 8 0.02876255330392752 1108 9 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-0.3572903044901068 2316 94-95 0.026304106153634876 2316 96-97 -0.2523871895396361 2316 98-99 -0.1225210715037619 2316 100-101 0.05646621591780843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 17.0 5 32.0 6 85.0 7 194.0 8 531.0 9 987.0 10 1632.0 11 2583.0 12 3732.0 13 5439.0 14 6868.0 15 9324.0 16 11924.0 17 16462.0 18 16842.0 19 12546.0 20 11630.0 21 11903.0 22 12458.0 23 13299.0 24 15331.0 25 18359.0 26 21383.0 27 25446.0 28 31200.0 29 37888.0 30 46441.0 31 57103.0 32 71526.0 33 93322.0 34 130776.0 35 197330.0 36 329985.0 37 535022.0 38 416420.0 39 37612.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55947454317606 18.19404270976015 14.40557332497887 23.840909422084923 2 16.278195949340205 19.455437614384557 39.02427845551975 25.242087980755485 3 19.972082566987996 22.810838970594574 29.084501513631306 28.13257694878612 4 13.883840425913297 15.88648060187662 34.76460094906566 35.46507802314443 5 14.65579012189372 36.370443280007954 33.36736791519127 15.606398682907061 6 34.34161916573525 34.4591973011938 16.26331149220767 14.935872040863279 7 30.129499314997314 31.70021795746222 19.399439382367635 18.770843345172832 8 27.373061220044466 34.62401543627116 18.57743616133481 19.425487182349574 9 26.56530714394949 16.74406130581161 19.387096174013827 37.30353537622507 10-11 26.062729818513997 25.65272655573208 26.0069811734307 22.277562452323224 12-13 26.800281897096667 22.71322778982602 26.833908063972306 23.652582249105002 14-15 23.305723429769458 24.30632811728896 24.895268802321638 27.492679650619944 16-17 23.246229753324386 27.410138625273838 25.81901681451169 23.52461480689008 18-19 23.338289525900436 25.795291927154178 27.960504447072925 22.90591409987246 20-21 23.690905188277036 25.0352484819442 28.280672759310615 22.993173570468148 22-23 23.421351299962197 24.908299491614105 28.272481769943468 23.397867438480226 24-25 23.4318080384239 25.145799303285465 28.27541030622902 23.146982352061613 26-27 22.939372748300176 25.490455901285753 28.11028978318788 23.459881567226187 28-29 23.0411396411666 25.897480578733106 27.95521139786381 23.10616838223649 30-31 23.15110862121271 25.44034783547883 27.85390800975054 23.554635533557924 32-33 22.85340488763823 25.423673819828185 27.905407459745028 23.817513832788553 34-35 23.48809846634224 25.568072869450177 27.7963640159436 23.147464648263984 36-37 23.66730466364214 25.183086520878344 27.75461605904888 23.39499275643064 38-39 23.1720560297374 25.493662147156016 27.71874743974711 23.615534383359474 40-41 23.51536078597699 25.410272627031503 27.808923935975756 23.26544265101575 42-43 23.396686253842077 25.516594259546903 27.456893275044564 23.629826211566456 44-45 23.3500932463503 25.65870905482305 27.713396184330758 23.2778015144959 46-47 23.827370026070795 25.882220805358468 27.045082739044705 23.245326429526028 48-49 23.548489933076258 26.062776036331 26.77346783882485 23.61526619176789 50-51 23.608284030381586 26.29447901481074 26.82339994667907 23.273837008128606 52-53 23.973329588015833 25.902500884332085 26.819097761182007 23.305071766470075 54-55 23.506755410704375 26.10034937632763 26.901546122984644 23.491349089983352 56-57 23.408576986904855 26.366204840080577 26.730511004287905 23.494707168726666 58-59 23.539814816075413 25.8925755341748 26.702636077691388 23.8649735720584 60-61 23.205937007791825 26.099965076691724 27.097537237346202 23.596560678170256 62-63 23.258309942887223 26.113290689732633 27.214044705439218 23.414354661940926 64-65 23.025494912521417 26.23014240583519 27.003922905123513 23.740439776519885 66-67 23.017454339010765 26.25076042085735 26.88795963058692 23.843825609544965 68-69 23.50577486370341 25.841406071134486 27.06742298877021 23.58539607639189 70-71 23.293540100225034 26.05914484255829 26.719824145584514 23.92749091163216 72-73 23.204051839659908 26.1479751012075 26.7569445295309 23.89102852960169 74-75 23.171809745779825 26.596687028422505 26.5137334075741 23.717769818223566 76-77 23.320898958673233 26.15459478756775 26.70614893280776 23.818357320951257 78-79 23.32639707138632 26.325485169627566 26.664924092517072 23.683193666469037 80-81 23.500538785645904 26.300227381383774 26.486675124168023 23.712558708802298 82-83 23.37409331503627 26.37892806058362 26.529906082322274 23.717072542057842 84-85 23.143492973674025 26.55083110179925 26.48310228390196 23.822573640624768 86-87 23.43894093479104 26.45845882952597 26.305750973759594 23.796849261923388 88-89 23.210834277257693 26.50382414666352 26.113139700501836 24.17220187557695 90-91 23.11503577320999 26.44210717179455 26.281880001831425 24.160977053164032 92-93 23.255035643283094 26.181011335330933 26.443100305358268 24.120852716027702 94-95 23.02296517445446 25.98914705253714 26.643881648598843 24.344006124409557 96-97 23.201669418929853 25.585678429471955 26.71544502938546 24.49720712221274 98-99 22.851712156804552 25.983188037626448 26.431112227461355 24.733987578107648 100-101 23.143495247713595 25.219371639483786 27.01262261906356 24.624510493739063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 319.0 1 222.5 2 191.5 3 279.0 4 253.0 5 223.5 6 401.0 7 908.0 8 1600.5 9 1879.5 10 1672.0 11 1508.5 12 1573.0 13 1693.0 14 1844.0 15 2005.0 16 2149.0 17 2266.0 18 2396.5 19 2748.0 20 3146.0 21 3719.0 22 4474.5 23 5481.0 24 6857.0 25 8507.0 26 10775.0 27 13617.5 28 16231.0 29 18502.0 30 21055.5 31 24906.0 32 30809.0 33 33043.5 34 32013.0 35 33331.5 36 36747.5 37 40645.5 38 44626.0 39 47891.5 40 51081.0 41 55424.5 42 57764.5 43 59830.0 44 63120.0 45 68594.5 46 73320.0 47 77008.5 48 81542.0 49 82676.0 50 81916.5 51 82695.5 52 85288.5 53 89132.5 54 91775.0 55 87881.0 56 81907.0 57 76029.0 58 70832.0 59 64610.0 60 54341.0 61 46211.0 62 39263.5 63 29530.5 64 21387.0 65 16610.0 66 12568.0 67 9803.0 68 7541.5 69 5638.0 70 4283.5 71 3054.5 72 2172.5 73 1769.0 74 1394.5 75 1083.5 76 811.5 77 528.5 78 329.0 79 201.0 80 123.5 81 85.0 82 57.5 83 30.5 84 17.5 85 11.0 86 6.0 87 4.0 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3452921778093619 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001066416898215272 16-17 0.0017471085353739561 18-19 2.2689721238622806E-5 20-21 0.0 22-23 0.0 24-25 5.672430309655702E-4 26-27 0.0012706243893628772 28-29 0.0 30-31 0.0031992506946458155 32-33 0.0 34-35 0.011276791455595535 36-37 0.02961008621640276 38-39 0.022213237092611728 40-41 0.014498731871479974 42-43 0.011049894243209306 44-45 0.00315387125216857 46-47 0.001179865504408386 48-49 6.126224734428157E-4 50-51 1.5882804867035964E-4 52-53 2.2689721238622806E-5 54-55 0.0 56-57 0.0 58-59 0.004628703132679052 60-61 0.010959135358254816 62-63 0.004810220902588035 64-65 0.00201938519023743 66-67 0.004152218986667974 68-69 0.003743804004372763 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21616497424035946 78-79 2.0420749114760526E-4 80-81 0.003380768464554798 82-83 4.084149822952105E-4 84-85 0.0 86-87 0.10795769365336731 88-89 0.4040131763749176 90-91 0.39173803718482275 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014294524380332367 100-101 7.941402433517982E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2203641.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.34765527311777 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37993983436319 38.19235509850122 2 13.844678213496023 14.21783528547676 3 4.730313068873604 7.286714543833369 4 2.2290761066052998 4.578313259980499 5 1.2115467831826672 3.110504328505918 6 0.767761623010488 2.3653655490163175 7 0.5115726736767314 1.8387640106569987 8 0.37460434573248147 1.538804384678663 9 0.28401500385913026 1.3125154059496653 >10 1.5155467867646488 14.171190728437915 >50 0.10113404745023805 3.5412560013272345 >100 0.044074407797691076 4.108572181307025 >500 0.002912684172267363 1.0261141523721222 >1k 0.0026478947020611536 2.1904575421778283 >5k 1.765263134707436E-4 0.5212375277784326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6152 0.279174330120015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5349 0.24273463781078677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3379 0.15333713613061292 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2714 0.1231598068832446 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2621 0.11893951873286075 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2592 0.11762351490102063 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2429 0.1102266657772296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.537944247724561E-4 2 0.0 0.0 0.0 0.0 4.537944247724561E-4 3 0.0 0.0 0.0 0.0 4.537944247724561E-4 4 0.0 0.0 0.0 0.0 4.537944247724561E-4 5 0.0 0.0 0.0 0.0 5.445533097269474E-4 6 0.0 0.0 0.0 0.0 5.445533097269474E-4 7 0.0 0.0 0.0 0.0 5.445533097269474E-4 8 0.0 0.0 0.0 0.0 5.445533097269474E-4 9 0.0 0.0 0.0 0.0 5.89932752204193E-4 10-11 0.0 0.0 0.0 0.0 5.89932752204193E-4 12-13 4.537944247724561E-5 0.0 0.0 0.0 0.0017017290928967106 14-15 9.075888495449123E-5 0.0 0.0 4.537944247724561E-5 0.001996695468998807 16-17 9.075888495449123E-5 0.0 0.0 4.537944247724561E-5 0.002223592681385035 18-19 1.3613832743173684E-4 4.537944247724561E-5 0.0 6.806916371586842E-5 0.0024051104512940176 20-21 1.8151776990898245E-4 4.537944247724561E-5 0.0 2.0420749114760526E-4 0.002677387106157491 22-23 1.8151776990898245E-4 4.537944247724561E-5 0.0 3.176560973407193E-4 0.0028815945973050964 24-25 2.2689721238622806E-4 4.537944247724561E-5 0.0 4.991738672497017E-4 0.002904284318543719 26-27 2.2689721238622806E-4 4.537944247724561E-5 0.0 6.126224734428157E-4 0.0029950432034982107 28-29 3.176560973407193E-4 4.537944247724561E-5 0.0 0.0017017290928967104 0.0031084918096913245 30-31 3.630355398179649E-4 4.537944247724561E-5 0.0 0.005241325606121869 0.0031311815309299474 32-33 3.630355398179649E-4 4.537944247724561E-5 0.0 0.012910451384776377 0.0032446301371230613 34-35 4.084149822952105E-4 4.537944247724561E-5 0.0 0.024709106428860234 0.0033580787433161756 36-37 4.537944247724561E-4 4.537944247724561E-5 0.0 0.045243304149813875 0.0033580787433161756 38-39 4.537944247724561E-4 4.537944247724561E-5 0.0 0.07521642590603461 0.0033580787433161756 40-41 4.537944247724561E-4 4.537944247724561E-5 0.0 0.11061239103828618 0.003403458185793421 42-43 5.89932752204193E-4 4.537944247724561E-5 0.0 0.1484134666218318 0.0034715273495092894 44-45 7.714505221131754E-4 4.537944247724561E-5 0.0 0.19656105509018937 0.003607665676941026 46-47 9.075888495449123E-4 4.537944247724561E-5 0.0 0.25417025731505266 0.0037438040043727633 48-49 9.075888495449123E-4 4.537944247724561E-5 0.0 0.3159997476902998 0.004152218986667974 50-51 9.075888495449123E-4 4.537944247724561E-5 0.0 0.38799423318045 0.00428835731409971 52-53 9.075888495449123E-4 4.537944247724561E-5 0.0 0.4574247801706358 0.004606013411440429 54-55 9.075888495449123E-4 4.537944247724561E-5 0.0 0.5374287372580198 0.004787531181349412 56-57 9.075888495449123E-4 4.537944247724561E-5 0.0 0.6336785347522578 0.004878290066303904 58-59 9.075888495449123E-4 6.806916371586842E-5 0.0 0.7445178230029301 0.0049463592300197715 60-61 9.075888495449123E-4 9.075888495449123E-5 0.0 0.8679499065410382 0.0049463592300197715 62-63 9.075888495449123E-4 9.075888495449123E-5 0.0 0.9970317306675633 0.005037118114974263 64-65 9.075888495449123E-4 9.075888495449123E-5 0.0 1.131264121515256 0.005400153654792228 66-67 9.075888495449123E-4 9.075888495449123E-5 0.0 1.292769557291773 0.005513602260985342 68-69 9.075888495449123E-4 9.075888495449123E-5 0.0 1.4805496902626154 0.005536291982223965 70-71 0.0010664168982152718 9.075888495449123E-5 0.0 1.6810360671270863 0.005649740588417079 72-73 0.0011571757831697633 9.075888495449123E-5 0.0 1.8935253065267892 0.005944706964519176 74-75 0.001179865504408386 9.075888495449123E-5 0.0 2.11114242292642 0.006194293898144026 76-77 0.001179865504408386 9.075888495449123E-5 0.0 2.357371277807955 0.006216983619382649 78-79 0.001179865504408386 9.075888495449123E-5 0.0 2.6448727356225445 0.006330432225575763 80-81 0.001406762716794614 9.075888495449123E-5 0.0 2.950321762936885 0.006443880831768877 82-83 0.0015882804867035966 9.075888495449123E-5 0.0 3.263802951569698 0.00703381358397307 84-85 0.0016109702079422192 9.075888495449123E-5 0.0 3.6057824300782206 0.007079193026450316 86-87 0.001633659929180842 9.075888495449123E-5 0.0 3.9597874608432138 0.007442228566268281 88-89 0.001633659929180842 9.075888495449123E-5 0.0 4.337911665284863 0.007759884663609 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4690 0.0 44.47462 1 GTATCAA 7620 0.0 36.435486 1 ATCAACG 9445 0.0 28.837076 3 TATCAAC 9670 0.0 28.264242 2 TCAACGC 9650 0.0 28.224478 4 CAACGCA 9785 0.0 27.835073 5 AACGCAG 10040 0.0 27.128109 6 ACGCAGA 11405 0.0 23.881298 7 TAAGGTA 825 0.0 23.58149 4 CGCAGAG 11580 0.0 23.561373 8 GTGTAAG 790 0.0 21.701332 1 GAGTACT 7430 0.0 21.012035 12-13 TACATGG 7405 0.0 20.95386 2 GCAGAGT 12980 0.0 20.837774 9 GTACATG 7625 0.0 20.7977 1 TAAGGTG 1380 0.0 20.630678 5 AGAGTAC 10880 0.0 20.302608 10-11 TGTAAGG 890 0.0 20.259789 2 GTACTTT 7710 0.0 19.910446 14-15 CAGAGTA 12050 0.0 19.630852 10-11 >>END_MODULE