FastQCFastQC Report
Fri 27 May 2016
SRR939216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168246
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.27243218712267886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.23184638643401162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.14431019358504524No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28090.12955172060734807No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26150.12060439636461914No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25610.11811390404963275No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25040.11548505105048043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39400.046.889151
GTATCAA66700.038.306761
ATCAACG82900.030.4541723
TATCAAC83200.030.3443622
TCAACGC84400.029.9129264
CAACGCA85800.029.4241565
AACGCAG88500.028.616996
GTCTACG1701.1256452E-725.1427381
ACGCAGA101050.024.9223357
CGCAGAG102150.024.653968
GTACATG72600.024.4654941
TACATGG71050.024.2667372
ACATGGG70800.023.1481883
TAAGGTG11800.021.6758355
GTAAGGT10200.021.3615024
GCAGAGT114450.021.34269
GAGTACT67900.020.88120312-13
AGAGTAC97150.020.44125610-11
GTACTTT70000.020.08615914-15
TGGTATC22600.019.7012392
Produced by FastQC (version 0.11.5)