##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2168246 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08152718833564 33.0 31.0 34.0 30.0 34.0 2 32.18535212332918 34.0 31.0 34.0 30.0 34.0 3 32.249562088434615 34.0 31.0 34.0 30.0 34.0 4 35.68654709843809 37.0 35.0 37.0 35.0 37.0 5 35.71722212331996 37.0 35.0 37.0 35.0 37.0 6 35.79538115140072 37.0 36.0 37.0 35.0 37.0 7 35.717547270927746 37.0 36.0 37.0 35.0 37.0 8 35.70564502367351 37.0 36.0 37.0 35.0 37.0 9 37.386755008426164 39.0 38.0 39.0 35.0 39.0 10-11 37.29278758037603 39.0 37.5 39.0 34.0 39.0 12-13 37.19115935184476 39.0 37.0 39.0 34.0 39.0 14-15 38.49095259486239 40.0 38.0 41.0 34.0 41.0 16-17 38.38686223795639 40.0 38.0 41.0 34.0 41.0 18-19 38.266235242680025 40.0 38.0 41.0 33.5 41.0 20-21 38.232877865334466 40.0 38.0 41.0 33.5 41.0 22-23 38.279637550351765 40.0 38.0 41.0 34.0 41.0 24-25 38.219703391589334 40.0 38.0 41.0 34.0 41.0 26-27 38.093446961276534 40.0 38.0 41.0 33.0 41.0 28-29 37.947049827371984 40.0 38.0 41.0 33.0 41.0 30-31 37.72661888918509 40.0 38.0 41.0 32.5 41.0 32-33 37.511880570747046 40.0 37.5 41.0 32.0 41.0 34-35 37.20129288835307 40.0 37.0 41.0 31.0 41.0 36-37 36.928475597326134 40.0 37.0 41.0 30.5 41.0 38-39 36.71829464922338 40.0 36.0 41.0 30.0 41.0 40-41 36.503437801799244 40.0 36.0 41.0 30.0 41.0 42-43 36.27527757459255 39.5 35.5 41.0 30.0 41.0 44-45 35.99740712077873 39.0 35.0 41.0 28.5 41.0 46-47 35.87187085782702 39.0 35.0 41.0 28.0 41.0 48-49 35.73526274232721 39.0 35.0 41.0 27.0 41.0 50-51 34.58012052137995 38.0 34.0 40.0 25.5 40.5 52-53 34.69865089108893 38.5 34.5 39.5 26.0 40.5 54-55 35.188879859573134 39.0 35.0 40.5 26.0 41.0 56-57 35.05745565770674 39.0 35.0 41.0 26.0 41.0 58-59 34.804409647244825 38.0 35.0 40.5 26.0 41.0 60-61 34.47645585417891 38.0 34.0 40.0 25.5 41.0 62-63 34.06431765583795 37.0 34.0 40.0 23.5 41.0 64-65 33.66426042063493 36.5 34.0 40.0 22.5 41.0 66-67 33.26392992308068 36.0 33.5 39.0 22.0 41.0 68-69 32.83253560712207 35.5 33.0 39.0 20.5 41.0 70-71 32.39088346064054 35.0 33.0 38.5 19.5 40.0 72-73 31.953053066856803 35.0 32.0 37.0 18.0 39.5 74-75 31.525051354873938 35.0 32.0 37.0 15.5 39.0 76-77 31.103088394951495 35.0 31.0 36.0 11.5 39.0 78-79 30.69977115142839 35.0 31.0 36.0 7.5 38.5 80-81 30.277751233024297 35.0 31.0 35.0 4.5 37.0 82-83 29.89802333314578 34.0 30.5 35.0 2.0 37.0 84-85 29.5377948350879 34.0 30.0 35.0 2.0 36.0 86-87 29.154448803318445 34.0 29.5 35.0 2.0 36.0 88-89 28.7433962751459 34.0 29.0 35.0 2.0 35.5 90-91 28.33556893452127 34.0 29.0 35.0 2.0 35.0 92-93 27.930853095082384 34.0 28.0 35.0 2.0 35.0 94-95 27.439509861888364 34.0 27.0 35.0 2.0 35.0 96-97 26.83897514396429 33.5 25.5 35.0 2.0 35.0 98-99 26.106323498348434 33.0 24.5 35.0 2.0 35.0 100-101 23.178450692402983 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11185302357480253 1101 2 0.15382423524312827 1101 3 0.11072825705617362 1101 4 0.10777335495032503 1101 5 0.06076410941845012 1101 6 0.08887022109556142 1101 7 0.04171096540812158 1101 8 0.22237378178007816 1101 9 0.27625485283245865 1101 10-11 0.28605561218873987 1101 12-13 0.21960116706769384 1101 14-15 0.34775406516278196 1101 16-17 0.2476202076217504 1101 18-19 0.3303877287444692 1101 20-21 0.345422569543679 1101 22-23 0.3333492777915623 1101 24-25 0.38374799028404993 1101 26-27 0.30365002670129115 1101 28-29 0.30497002521084937 1101 30-31 0.23832538793345037 1101 32-33 0.2567772315389547 1101 34-35 0.288356810012516 1101 36-37 0.18968368843217576 1101 38-39 0.12954189533694915 1101 40-41 0.19414995330255636 1101 42-43 0.19622634178013243 1101 44-45 0.1343286371671013 1101 46-47 0.3289039169685495 1101 48-49 0.33899877227703 1101 50-51 0.2558250850209731 1101 52-53 0.36948103626613005 1101 54-55 0.45013036115364 1101 56-57 0.3493609364615793 1101 58-59 0.2776500773765562 1101 60-61 0.26300292238811807 1101 62-63 0.0023046289425963096 1101 64-65 0.11711591404780819 1101 66-67 0.09421629550881505 1101 68-69 0.04032701443990305 1101 70-71 -0.030980666917002964 1101 72-73 -0.011876321912140497 1101 74-75 0.02132320886723349 1101 76-77 0.06948161396560337 1101 78-79 -0.09684837186420125 1101 80-81 -0.1190913398672997 1101 82-83 -0.0974861626136807 1101 84-85 -0.24085672418544846 1101 86-87 -0.18316867639322254 1101 88-89 -0.27865437820535277 1101 90-91 -0.3358127775380453 1101 92-93 -0.3419026220898438 1101 94-95 -0.5166428544620878 1101 96-97 -0.6176894058023876 1101 98-99 -0.6387271801108234 1101 100-101 -1.028464752210244 1102 1 0.3539328283891905 1102 2 0.386116389519195 1102 3 0.4337916498246628 1102 4 0.3441394414504586 1102 5 0.39468328328400304 1102 6 0.32297947397156435 1102 7 0.44025132882784135 1102 8 0.40236300117948787 1102 9 0.545412405636128 1102 10-11 0.4757580108723758 1102 12-13 0.5229842897537651 1102 14-15 0.5839953236560476 1102 16-17 0.6128038324568479 1102 18-19 0.5925932126657685 1102 20-21 0.6269416412772202 1102 22-23 0.7109008048068972 1102 24-25 0.7392014138339817 1102 26-27 0.6059749483291625 1102 28-29 0.5435035798301939 1102 30-31 0.5932951029567874 1102 32-33 0.6436511410670178 1102 34-35 0.663049350120609 1102 36-37 0.6354359899485473 1102 38-39 0.4913700582374929 1102 40-41 0.5285209194429612 1102 42-43 0.5713146434099912 1102 44-45 0.5800532779319596 1102 46-47 0.6837771739865843 1102 48-49 0.6442514780659323 1102 50-51 0.620217186812539 1102 52-53 0.7542903189306429 1102 54-55 0.710172632455965 1102 56-57 0.7052923945378069 1102 58-59 0.6259606437836354 1102 60-61 0.6641710572023669 1102 62-63 0.6179506848031266 1102 64-65 0.5463448174054051 1102 66-67 0.4711046737376776 1102 68-69 0.3625797344138064 1102 70-71 0.5432799399173334 1102 72-73 0.5080926129361529 1102 74-75 0.45734459984841536 1102 76-77 0.33067499808123557 1102 78-79 0.377535644476918 1102 80-81 0.34086227518014667 1102 82-83 0.06732581520097725 1102 84-85 0.063598784559602 1102 86-87 -0.12745141182877262 1102 88-89 0.01850294763829652 1102 90-91 0.05270730133056034 1102 92-93 -0.12725944578447113 1102 94-95 -0.12514818092988023 1102 96-97 -0.12895229642184702 1102 98-99 -0.121449849160566 1102 100-101 -0.49397250431316664 1103 1 0.1429566667131823 1103 2 0.1833951835320491 1103 3 0.20865978612359015 1103 4 0.12079236631489465 1103 5 0.15152332706028915 1103 6 0.1170268873035809 1103 7 0.2378839536766364 1103 8 0.28614767454425305 1103 9 0.1729536104712679 1103 10-11 0.23157976241729017 1103 12-13 0.2948053665515147 1103 14-15 0.31336822021401645 1103 16-17 0.39406108393183104 1103 18-19 0.3491617118470245 1103 20-21 0.2798455227192065 1103 22-23 0.32606327898958654 1103 24-25 0.46412427048312566 1103 26-27 0.3718557813950625 1103 28-29 0.17518295972107722 1103 30-31 0.32605243260369576 1103 32-33 0.365129084240138 1103 34-35 0.32069086188342766 1103 36-37 0.30953228130767485 1103 38-39 0.3554669256003038 1103 40-41 0.15891951399396476 1103 42-43 0.20659816065307268 1103 44-45 0.10581339708243576 1103 46-47 0.14700592150771286 1103 48-49 0.15514946781306804 1103 50-51 0.06241645117354011 1103 52-53 0.1314205039924019 1103 54-55 0.17934162370893603 1103 56-57 0.20178124063785674 1103 58-59 0.226870318176978 1103 60-61 0.22896036328249636 1103 62-63 0.11739113836488713 1103 64-65 -0.07683891954194166 1103 66-67 0.05867286845983699 1103 68-69 -0.08109985942130749 1103 70-71 -0.08203171922014008 1103 72-73 -0.10979353582091278 1103 74-75 -0.29707705130730133 1103 76-77 -0.2697936220607886 1103 78-79 -0.2246453523386016 1103 80-81 -0.1975156161526428 1103 82-83 -0.30784060872127483 1103 84-85 -0.3836573807369952 1103 86-87 -0.3420580424540667 1103 88-89 -0.5415750597180917 1103 90-91 -0.37821010796943355 1103 92-93 -0.5277556318172039 1103 94-95 -0.705410180320964 1103 96-97 -0.6739850874888518 1103 98-99 -0.6978797783121173 1103 100-101 -0.8325252539079067 1104 1 0.1258580899009729 1104 2 0.2759686789120721 1104 3 0.20956322100204972 1104 4 0.17249615081789216 1104 5 0.22268509826897542 1104 6 0.2518482683840091 1104 7 0.2733124144157273 1104 8 0.23842043248959754 1104 9 0.28288131186460674 1104 10-11 0.41206945905211967 1104 12-13 0.3544872431556456 1104 14-15 0.39000259980115715 1104 16-17 0.3337972936493898 1104 18-19 0.4103249676103857 1104 20-21 0.4674170214922668 1104 22-23 0.5483733572105365 1104 24-25 0.5172927626757158 1104 26-27 0.39923033454014245 1104 28-29 0.4833951668887053 1104 30-31 0.49564891158931346 1104 32-33 0.39390880411181683 1104 34-35 0.45240738021370674 1104 36-37 0.4805740179349698 1104 38-39 0.30219919846938836 1104 40-41 0.4957292456865119 1104 42-43 0.44166151620709115 1104 44-45 0.28537385775483415 1104 46-47 0.534096011747998 1104 48-49 0.643270251816709 1104 50-51 0.5708613444245358 1104 52-53 0.590986236024726 1104 54-55 0.45300141617295253 1104 56-57 0.5043743686580697 1104 58-59 0.5799033745257489 1104 60-61 0.6229218775917786 1104 62-63 0.4520173044746514 1104 64-65 0.5604299920571876 1104 66-67 0.3814646727653681 1104 68-69 0.3015504596785519 1104 70-71 0.27062503824252815 1104 72-73 0.29588643388464675 1104 74-75 0.287735874956379 1104 76-77 0.10828950823785988 1104 78-79 0.23611874080886608 1104 80-81 0.21934376476423978 1104 82-83 0.019930804789122902 1104 84-85 -0.01970173319726598 1104 86-87 0.027280108140114834 1104 88-89 -0.07473461282744154 1104 90-91 -7.545394207149059E-4 1104 92-93 -0.09305103041219454 1104 94-95 -0.059278000970881095 1104 96-97 0.0430361654594158 1104 98-99 -0.2315910776785195 1104 100-101 -0.34811982599494584 1105 1 0.10644618778191273 1105 2 0.12661658206325654 1105 3 0.061838198010789824 1105 4 0.20432765986400625 1105 5 0.1703378012117014 1105 6 0.2672904563682934 1105 7 0.14767350673854907 1105 8 0.1693593665806361 1105 9 0.15934151777062056 1105 10-11 0.1206975309365177 1105 12-13 0.0328428094328288 1105 14-15 0.06031504175044944 1105 16-17 0.04035767885416419 1105 18-19 0.13056000013405367 1105 20-21 0.11834415331782822 1105 22-23 0.12589576701023475 1105 24-25 0.27166988144702486 1105 26-27 0.26220572689294386 1105 28-29 0.17688043339647663 1105 30-31 0.17745558447519727 1105 32-33 0.15281966723454588 1105 34-35 0.22619958196475665 1105 36-37 0.250890684067258 1105 38-39 0.1555157165007941 1105 40-41 0.23549628962869207 1105 42-43 0.2172688442993831 1105 44-45 -0.012308261787509878 1105 46-47 0.15054248651460966 1105 48-49 0.24071948146315236 1105 50-51 0.14303049292594494 1105 52-53 0.1859780599404175 1105 54-55 0.18252647041299497 1105 56-57 0.1549476615410299 1105 58-59 0.14239150991539162 1105 60-61 0.10541162122458303 1105 62-63 0.06037077901784471 1105 64-65 0.15720984138109628 1105 66-67 0.06733661284100378 1105 68-69 0.09072835732812479 1105 70-71 -0.010158949762825387 1105 72-73 0.10993866209027914 1105 74-75 -0.03881955319510766 1105 76-77 -0.06634241528712437 1105 78-79 -0.09065502162002659 1105 80-81 -0.05117431346661405 1105 82-83 -0.04438059994511789 1105 84-85 -0.16253471968955324 1105 86-87 -0.21312692497188124 1105 88-89 -0.15691049457474548 1105 90-91 -0.06708464558003513 1105 92-93 -0.15708188323402794 1105 94-95 -0.20148003193770236 1105 96-97 -0.12165645906619105 1105 98-99 -0.01740642388085334 1105 100-101 -0.27961452006485743 1106 1 0.22625604183114945 1106 2 0.1827460347258807 1106 3 0.31037570619281496 1106 4 0.288217305940492 1106 5 0.2616189137132565 1106 6 0.2223678150674644 1106 7 0.2945420277701558 1106 8 0.2690680912408112 1106 9 0.2818180528366696 1106 10-11 0.36161975934452073 1106 12-13 0.3408706395831942 1106 14-15 0.4084666125532479 1106 16-17 0.4440007731238609 1106 18-19 0.5159878676600371 1106 20-21 0.4193831364665144 1106 22-23 0.43237818433137676 1106 24-25 0.4318069873031476 1106 26-27 0.48723549529137955 1106 28-29 0.41113913912424493 1106 30-31 0.37780424409007196 1106 32-33 0.4468981775328942 1106 34-35 0.3942198097994094 1106 36-37 0.5783614122164664 1106 38-39 0.5219649458490636 1106 40-41 0.6402789974424508 1106 42-43 0.693510384644 1106 44-45 0.6371958754572873 1106 46-47 0.40521223585344046 1106 48-49 0.5120994045007095 1106 50-51 0.6219641531816862 1106 52-53 0.5170686938287403 1106 54-55 0.4880404975171615 1106 56-57 0.434452376053045 1106 58-59 0.4692321362209739 1106 60-61 0.5939831979566108 1106 62-63 0.43593153541400653 1106 64-65 0.3690094279012257 1106 66-67 0.3395782784232537 1106 68-69 0.46438926860802354 1106 70-71 0.4610603657939407 1106 72-73 0.498756506887581 1106 74-75 0.34333330129343764 1106 76-77 0.39973481915283315 1106 78-79 0.33956658050196964 1106 80-81 0.24405946507998877 1106 82-83 0.20140632778983303 1106 84-85 0.2736833679513495 1106 86-87 0.2923735021080134 1106 88-89 0.34616467637951587 1106 90-91 0.3360107786477755 1106 92-93 0.25475840551699136 1106 94-95 0.23649774229602372 1106 96-97 0.31778405159026235 1106 98-99 0.4351524842818044 1106 100-101 0.17378118095768968 1107 1 0.10771805048836569 1107 2 0.14637078843638562 1107 3 0.15437888988864046 1107 4 0.17737895172485452 1107 5 0.12987923504972798 1107 6 0.2364638470489666 1107 7 0.20921127714825616 1107 8 0.24000450480936308 1107 9 0.33892630990584394 1107 10-11 0.2128573402682079 1107 12-13 0.2017218312820077 1107 14-15 0.1931261529903665 1107 16-17 0.2089809273258041 1107 18-19 0.23091301086929406 1107 20-21 0.25273144686354243 1107 22-23 0.33322146433685873 1107 24-25 0.2854454153105479 1107 26-27 0.29328038083808394 1107 28-29 0.41757380869693606 1107 30-31 0.448183130519233 1107 32-33 0.3972064197676488 1107 34-35 0.43219617551743283 1107 36-37 0.4781700861710192 1107 38-39 0.4307665144944153 1107 40-41 0.4739128229743983 1107 42-43 0.4575434178655087 1107 44-45 0.5117800836413267 1107 46-47 0.6726322250509398 1107 48-49 0.6197951903829022 1107 50-51 0.513819530850931 1107 52-53 0.5518670692690222 1107 54-55 0.45451018013653766 1107 56-57 0.5626144383424077 1107 58-59 0.5562854868991778 1107 60-61 0.42722476705839796 1107 62-63 0.48495265354928563 1107 64-65 0.3917144187343027 1107 66-67 0.40887691441689356 1107 68-69 0.47219659795508306 1107 70-71 0.4252820624717799 1107 72-73 0.4326104239365236 1107 74-75 0.3617981189821613 1107 76-77 0.44204835233249895 1107 78-79 0.46557551158655386 1107 80-81 0.4202527329136281 1107 82-83 0.5438885074613431 1107 84-85 0.5380727601963713 1107 86-87 0.590791020803195 1107 88-89 0.5915072850321685 1107 90-91 0.30006487485092137 1107 92-93 0.14285406473601725 1107 94-95 0.2892498134099739 1107 96-97 0.47071009437426525 1107 98-99 0.5738343885655297 1107 100-101 0.5908158554751886 1108 1 0.016309016357794803 1108 2 0.11359451083617955 1108 3 0.08408378352911683 1108 4 0.17240494459748135 1108 5 0.12950862995769086 1108 6 0.09748234515690513 1108 7 0.03050935937022814 1108 8 0.1354355456264429 1108 9 0.21774120023980004 1108 10-11 0.19622993845330683 1108 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8 4107.0 9 5015.0 10 6025.0 11 7298.0 12 9091.0 13 13473.0 14 11755.0 15 11709.0 16 13855.0 17 18506.0 18 16304.0 19 13122.0 20 12724.0 21 13062.0 22 14284.0 23 15761.0 24 17435.0 25 19394.0 26 22112.0 27 26263.0 28 30226.0 29 36345.0 30 43654.0 31 53584.0 32 67371.0 33 88699.0 34 123836.0 35 188672.0 36 311317.0 37 474971.0 38 393754.0 39 63443.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34159179598874 17.70258607118317 13.966506831428461 23.989315301399625 2 15.907213500650826 18.978594941226937 39.78347908277358 25.330712475348648 3 19.956401402617026 22.62403343821254 28.855234371320414 28.564330787850018 4 13.553337351038946 15.422124627380896 35.06741202032867 35.957126001251495 5 14.74782134824715 36.23609334373975 33.38285619282049 15.633229115192604 6 34.46094669121465 33.971571663233554 16.497056965136355 15.070424680415446 7 30.21042010166043 31.64979403885105 19.43817195863421 18.701613900854312 8 27.32172134174261 34.36479919649055 18.867747736437085 19.445731725329747 9 26.699413846407356 16.730279340336036 19.469909259048134 37.10039755420848 10-11 26.0666988531043 25.513236749403656 26.061760111957106 22.358304285534935 12-13 26.84285630870914 22.63810416424816 26.812207139456255 23.70683238758644 14-15 23.422977671456508 24.167716314832603 24.876730765591603 27.53257524811929 16-17 23.35032186984061 27.290809989481012 25.802597575233357 23.55627056544502 18-19 23.511806738273293 25.71057502181689 27.85452700689393 22.923091233015892 20-21 23.796920915194995 25.02610371877594 28.146040497461637 23.030934868567428 22-23 23.54577056778679 24.8504475723987 28.13649226962371 23.467289590190802 24-25 23.529896562598257 25.10696719211063 28.139193324009824 23.22394292128129 26-27 22.9796288588204 25.44260673777555 28.028008616544163 23.549755786859887 28-29 23.069283869006302 25.91062172906457 27.821975699825224 23.1981187021039 30-31 23.228932960326475 25.45220157142989 27.675977988902122 23.64288747934151 32-33 22.882288203430328 25.396915159500068 27.783368710085742 23.937427926983858 34-35 23.55247858778286 25.558372081453772 27.628777001722693 23.26037232904067 36-37 23.778729836372655 25.096558215446535 27.64541690429493 23.479295043885884 38-39 23.200028556534406 25.479799612864255 27.60441040839321 23.71576142220813 40-41 23.64278232982352 25.351336571259083 27.703984687824413 23.301896411092983 42-43 23.42977740021719 25.466425759315943 27.358761498245926 23.745035342220945 44-45 23.38765910374301 25.67165030232841 27.594386640988144 23.346303952940435 46-47 23.933947341092342 25.859304878682003 26.924708480761954 23.282039299463694 48-49 23.65248423425042 26.02452741002169 26.654114186612972 23.66887416911492 50-51 23.584597078007956 26.28425104104341 26.744204106867862 23.38694777408077 52-53 24.003882470649653 25.854987356702342 26.7627826196616 23.378347552986405 54-55 23.538884385443517 26.0828687006271 26.869613009422157 23.508633904507228 56-57 23.426503419722835 26.3541356237466 26.677175105474475 23.54218585105609 58-59 23.537489019683356 25.85636042734431 26.69852261881508 23.90762793415726 60-61 23.181037587385255 26.115005193611108 27.044366292595427 23.65959092640821 62-63 23.296344487156965 26.09692965846002 27.19954925493101 23.407176599452004 64-65 23.084277442255168 26.205221139292124 26.986975550961713 23.723525867490995 66-67 23.009386726887634 26.230922523855178 26.867624776692818 23.89206597256437 68-69 23.53380128512827 25.809141524112967 27.07903469289794 23.578022497860825 70-71 23.36284051790303 26.045176229217397 26.693760779405196 23.898222473474373 72-73 23.307483786844184 26.107029549537188 26.81055021325412 23.77493645036451 74-75 23.273074212156622 26.57568675458609 26.555561738356243 23.595677294901044 76-77 23.462108960021475 26.10591786216218 26.65041894594882 23.78155423186752 78-79 23.48508162743186 26.274397388218805 26.64160959729478 23.598911387054553 80-81 23.706034465576835 26.231299163784026 26.44518523428277 23.617481136356368 82-83 23.58165517660194 26.383634729749183 26.48849513954385 23.546214954105025 84-85 23.379159643921316 26.560648706980473 26.435120031002057 23.625071618096154 86-87 23.671168728102746 26.44914738039545 26.290379254174557 23.589304637327242 88-89 23.45506763961391 26.482392374665046 26.101148807150604 23.961391178570434 90-91 23.387015629412247 26.4455942788756 26.278528766288655 23.888861325423495 92-93 23.601470063489288 26.15622821272656 26.427616345054535 23.814685378729617 94-95 23.40521180764378 25.9283697555832 26.694713862495785 23.97170457427723 96-97 23.65551884128399 25.500047760496415 26.731712134968383 24.11272126325122 98-99 23.354213732189294 25.899855696643925 26.46065415048355 24.285276420683232 100-101 23.90311802072963 25.096572529939515 26.887553994272288 24.11275545505857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1829.0 1 1027.0 2 272.5 3 361.0 4 341.0 5 310.0 6 496.0 7 1030.0 8 1701.5 9 2021.5 10 1879.0 11 1738.0 12 1858.0 13 2096.5 14 2357.0 15 2564.5 16 2729.5 17 2875.0 18 3135.0 19 3413.0 20 3679.5 21 4105.0 22 4786.0 23 5723.0 24 6933.0 25 8530.0 26 10671.0 27 13419.0 28 15831.0 29 17814.0 30 19965.5 31 23677.5 32 29610.5 33 31712.5 34 30934.5 35 32500.5 36 35799.0 37 39877.5 38 43794.0 39 46692.0 40 49821.5 41 54181.0 42 56467.0 43 58406.0 44 62007.0 45 67756.5 46 71769.0 47 74750.0 48 79694.5 49 80850.5 50 79889.0 51 80567.0 52 83085.5 53 87060.0 54 89416.5 55 85502.5 56 79910.0 57 74416.0 58 69460.0 59 63194.0 60 53061.0 61 45574.0 62 39101.5 63 29775.5 64 21665.0 65 16787.0 66 12951.0 67 10088.5 68 7840.5 69 6025.0 70 4599.0 71 3308.0 72 2385.0 73 1968.0 74 1541.0 75 1180.5 76 912.0 77 628.0 78 405.0 79 280.0 80 201.0 81 152.5 82 113.5 83 80.5 84 58.5 85 41.0 86 31.0 87 23.5 88 21.5 89 16.5 90 9.5 91 7.5 92 7.0 93 7.0 94 5.5 95 6.0 96 5.0 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39303658348729803 2 0.11728373994463728 3 0.11899018838268352 4 0.13056636562456472 5 0.13448658500926555 6 0.07402296602876242 7 0.0838465746045421 8 0.08071039909678145 9 0.08066427886872615 10-11 0.07849662815012688 12-13 0.08267050878913186 14-15 0.07596001560708517 16-17 0.07780482472929733 18-19 0.08852777775215542 20-21 0.08707499056841336 22-23 0.0975904025650226 24-25 0.11045794619245232 26-27 0.11126505018342016 28-29 0.10582286327289431 30-31 0.09528439116225741 32-33 0.09408526523281951 34-35 0.3407593049866113 36-37 0.5311205462848773 38-39 0.51292611631706 40-41 0.48361671138791446 42-43 0.5130183567731705 44-45 0.5220809815860378 46-47 0.5197749701832726 48-49 0.5273617476983701 50-51 0.5255630588042132 52-53 0.5244792334449135 54-55 0.5191062268764707 56-57 0.48956622080704865 58-59 0.47999627348557317 60-61 0.43092435083473 62-63 0.504047972416414 64-65 0.5301289613816883 66-67 0.541612898167459 68-69 0.5553336660139117 70-71 0.5504449218400496 72-73 0.5379463400370622 74-75 0.5408057941764911 76-77 0.5177226200348116 78-79 0.4906269860523206 80-81 0.4975911404886715 82-83 0.5115886297034562 84-85 0.5059389017666814 86-87 0.5078759513450043 88-89 0.5177456801488391 90-91 0.5353174870379099 92-93 0.541474537483293 94-95 0.5369778152479008 96-97 0.5373928973003985 98-99 0.5385920232298365 100-101 0.5160161715967653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2168246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.3699123099781 #Duplication Level Percentage of deduplicated Percentage of total 1 76.19681053416535 40.666170965084206 2 12.83547621772029 13.700564803930822 3 4.452184403380273 7.12838073588772 4 2.0676417146607515 4.413994279995882 5 1.1628142392818337 3.102964699163268 6 0.6776840298687083 2.170076234877932 7 0.482000047473695 1.8007010186953467 8 0.33894030457554636 1.4471371466811342 9 0.2513757606880644 1.2074312074298434 >10 1.4007957104014517 13.566996530509545 >50 0.08780331857775374 3.2112934037041065 >100 0.041111123644560675 3.9686900564890144 >500 0.0027677912576763862 1.04348054300986 >1k 0.0024218173504668124 2.06738861310503 >5k 1.729869536047723E-4 0.5047297614363953 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5907 0.27243218712267886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5027 0.23184638643401162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3129 0.14431019358504524 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2809 0.12955172060734807 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2615 0.12060439636461914 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2561 0.11811390404963275 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2504 0.11548505105048043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.612022805530369E-4 2 0.0 0.0 0.0 0.0 4.612022805530369E-4 3 0.0 0.0 0.0 0.0 4.612022805530369E-4 4 0.0 0.0 0.0 0.0 4.612022805530369E-4 5 4.6120228055303685E-5 0.0 0.0 0.0 4.612022805530369E-4 6 9.224045611060737E-5 0.0 0.0 0.0 5.073225086083406E-4 7 1.3836068416591107E-4 0.0 0.0 0.0 5.073225086083406E-4 8 1.3836068416591107E-4 0.0 0.0 0.0 5.073225086083406E-4 9 1.3836068416591107E-4 0.0 0.0 0.0 5.073225086083406E-4 10-11 1.3836068416591107E-4 0.0 0.0 0.0 5.073225086083406E-4 12-13 1.614207981935629E-4 0.0 0.0 2.3060114027651843E-5 0.0011760658154102441 14-15 1.8448091222121474E-4 0.0 0.0 4.6120228055303685E-5 0.0013605467276314588 16-17 2.0754102624886658E-4 0.0 0.0 1.1530057013825922E-4 0.0015680877538803255 18-19 2.7672136833182214E-4 0.0 0.0 2.3060114027651844E-4 0.0016603282099909328 20-21 3.228415963871258E-4 0.0 0.0 2.7672136833182214E-4 0.001960109692350407 22-23 3.228415963871258E-4 0.0 0.0 3.689618244424295E-4 0.002213770946654577 24-25 4.150820524977332E-4 0.0 0.0 4.150820524977332E-4 0.0023521316308204882 26-27 4.150820524977332E-4 0.0 0.0 5.303826226359924E-4 0.0024443720869310953 28-29 6.226230787465998E-4 0.0 0.0 0.0018909293502674513 0.0024904923149863993 30-31 7.37923648884859E-4 0.0 0.0 0.00599562964718948 0.0025135524290140513 32-33 7.609837629125109E-4 0.0 0.0 0.013282625679927463 0.0025596726570693545 34-35 7.840438769401627E-4 0.0 0.0 0.024720442237642776 0.002813333911373525 36-37 8.301641049954664E-4 0.0 0.0 0.0438142166525385 0.0028363940254011767 38-39 9.685247891613774E-4 0.0 0.0 0.07529127230028326 0.002951694595539436 40-41 9.685247891613774E-4 0.0 0.0 0.11140341086758607 0.002951694595539436 42-43 9.915849031890294E-4 0.0 0.0 0.14924505798696275 0.002974754709567088 44-45 0.0011068854733272885 0.0 0.0 0.19822474018169522 0.0031822957358159545 46-47 0.0012452461574931997 0.0 0.0 0.25795043551331354 0.0035051373322030803 48-49 0.0012913663855485032 0.0 0.0 0.32228815365046215 0.003920219384700814 50-51 0.0012913663855485032 0.0 0.0 0.3919527581279984 0.003966339612756117 52-53 0.0012913663855485032 0.0 0.0 0.46150206203539634 0.004243060981087939 54-55 0.0012913663855485032 0.0 0.0 0.5430195651231456 0.00438142166525385 56-57 0.001337486613603807 0.0 0.0 0.6413017711089978 0.004473662121364458 58-59 0.001337486613603807 0.0 0.0 0.747332175408141 0.004519782349419761 60-61 0.001337486613603807 0.0 0.0 0.8729406165167606 0.004519782349419761 62-63 0.0013605467276314588 0.0 0.0 0.9974652322660806 0.0046581430335856724 64-65 0.0013836068416591106 0.0 0.0 1.1318365167052078 0.004842623945806887 66-67 0.0014297270697144144 0.0 0.0 1.2926116317059964 0.004957924515945147 68-69 0.0014297270697144144 0.0 0.0 1.4808282823996908 0.005004044744000451 70-71 0.001475847297769718 0.0 0.0 1.6877236254557832 0.005073225086083405 72-73 0.00149890741179737 0.0 0.0 1.9018137240885027 0.005488307138581139 74-75 0.0015219675258250217 0.0 0.0 2.121369069745776 0.005926449305106524 76-77 0.0015219675258250217 0.0 0.0 2.365252835702222 0.006041749875244783 78-79 0.0015219675258250217 0.0 0.0 2.642320105744459 0.006087870103300087 80-81 0.0015680877538803255 0.0 0.0 2.9507721909783298 0.006203170673438346 82-83 0.0015680877538803255 0.0 0.0 3.280854663170138 0.006687433068019034 84-85 0.0015911478679079773 0.0 0.0 3.6175784482019107 0.006733553296074338 86-87 0.001614207981935629 0.0 0.0 3.9719662805788643 0.007010274664406161 88-89 0.001614207981935629 0.0 0.0 4.359168655217166 0.007310056146765635 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3940 0.0 46.88915 1 GTATCAA 6670 0.0 38.30676 1 ATCAACG 8290 0.0 30.454172 3 TATCAAC 8320 0.0 30.344362 2 TCAACGC 8440 0.0 29.912926 4 CAACGCA 8580 0.0 29.424156 5 AACGCAG 8850 0.0 28.61699 6 GTCTACG 170 1.1256452E-7 25.142738 1 ACGCAGA 10105 0.0 24.922335 7 CGCAGAG 10215 0.0 24.65396 8 GTACATG 7260 0.0 24.465494 1 TACATGG 7105 0.0 24.266737 2 ACATGGG 7080 0.0 23.148188 3 TAAGGTG 1180 0.0 21.675835 5 GTAAGGT 1020 0.0 21.361502 4 GCAGAGT 11445 0.0 21.3426 9 GAGTACT 6790 0.0 20.881203 12-13 AGAGTAC 9715 0.0 20.441256 10-11 GTACTTT 7000 0.0 20.086159 14-15 TGGTATC 2260 0.0 19.701239 2 >>END_MODULE