FastQCFastQC Report
Fri 27 May 2016
SRR939216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168246
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60380.27847393699792367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.24623589758726638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.14929117821501803No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26440.12194188297822296No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26400.12175740206600175No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25970.11977423225962368No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24170.111472591209669No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21690.10003477465195369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.045.6201361
GTATCAA74850.037.76221
ATCAACG93550.029.5714023
TCAACGC94000.029.4298344
TATCAAC94350.029.3709552
CAACGCA96400.028.6479175
AACGCAG98400.028.1138656
ACGCAGA112450.024.5168027
CGCAGAG113700.024.2472698
GTAAGGT10800.022.846874
GTACATG77600.022.1737881
TACATGG75850.021.8957392
ACATGGG75550.021.4174163
GCAGAGT128750.021.3028539
GAGTACT74600.020.86470212-13
AGAGTAC110100.020.731710-11
TAAGGTG12650.020.630955
GTACTTT75850.020.36444514-15
AGTACTT79950.019.79495812-13
TGGTATC24850.019.66792
Produced by FastQC (version 0.11.5)