##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939216_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2168246 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50814114265632 34.0 31.0 34.0 31.0 34.0 2 32.732182602896536 34.0 31.0 34.0 31.0 34.0 3 32.83679158176702 34.0 31.0 34.0 31.0 34.0 4 36.28610314512283 37.0 37.0 37.0 35.0 37.0 5 36.24069409098414 37.0 37.0 37.0 35.0 37.0 6 36.275322080612625 37.0 37.0 37.0 35.0 37.0 7 36.23200411761396 37.0 37.0 37.0 35.0 37.0 8 36.20316606141554 37.0 37.0 37.0 35.0 37.0 9 37.95477911639178 39.0 38.0 39.0 35.0 39.0 10-11 37.87048148595686 39.0 38.0 39.0 35.0 39.0 12-13 37.784127354552936 39.0 38.0 39.0 35.0 39.0 14-15 39.212939168341606 40.5 39.0 41.0 36.0 41.0 16-17 39.297267929930456 41.0 39.0 41.0 36.0 41.0 18-19 39.31705765858671 41.0 39.0 41.0 36.0 41.0 20-21 39.27092405566527 41.0 39.0 41.0 36.0 41.0 22-23 39.201309952837455 41.0 39.0 41.0 36.0 41.0 24-25 39.12032421597918 41.0 39.0 41.0 35.5 41.0 26-27 38.97080382023073 40.0 38.5 41.0 35.0 41.0 28-29 38.81607483652685 40.0 38.0 41.0 35.0 41.0 30-31 38.60887164094849 40.0 38.0 41.0 34.5 41.0 32-33 38.51389095148798 40.0 38.0 41.0 34.0 41.0 34-35 38.40946875954112 40.0 38.0 41.0 34.0 41.0 36-37 38.25033990608077 40.0 38.0 41.0 34.0 41.0 38-39 38.10126964375813 40.0 38.0 41.0 33.5 41.0 40-41 37.891691948238346 40.0 37.5 41.0 33.0 41.0 42-43 37.68687455205728 40.0 37.0 41.0 33.0 41.0 44-45 37.429803398691845 40.0 37.0 41.0 32.5 41.0 46-47 37.1102647024369 40.0 36.5 41.0 31.5 41.0 48-49 36.735067653762535 39.5 36.0 41.0 31.0 41.0 50-51 36.43782462875522 39.0 35.5 41.0 30.0 41.0 52-53 36.126056499124175 39.0 35.0 40.5 30.0 41.0 54-55 35.82933855291327 39.0 35.0 40.0 29.0 41.0 56-57 35.5259334042355 38.0 35.0 40.0 28.5 41.0 58-59 35.13106677009897 38.0 34.5 40.0 28.0 41.0 60-61 34.704526838744314 37.0 34.0 40.0 27.0 41.0 62-63 34.256931870276716 37.0 34.0 39.5 26.0 41.0 64-65 33.7633059163951 36.0 33.0 39.0 26.0 40.5 66-67 33.303834528000976 35.5 33.0 39.0 25.0 40.0 68-69 32.78030744666427 35.0 32.5 38.0 23.5 40.0 70-71 32.245568076685025 35.0 32.0 37.5 22.0 39.5 72-73 31.66878988823224 35.0 31.0 37.0 20.0 39.0 74-75 31.12398131946283 34.5 31.0 36.0 18.5 39.0 76-77 29.187303008975917 32.5 28.5 34.5 14.0 36.0 78-79 30.167760254140905 34.0 30.0 35.0 15.0 37.0 80-81 30.205224407193647 34.0 30.0 35.0 13.5 37.0 82-83 29.82462898582541 34.0 30.0 35.0 7.5 36.0 84-85 29.354754718791135 34.0 29.5 35.0 4.5 36.0 86-87 28.64341200214367 33.5 29.0 35.0 2.0 35.5 88-89 27.93538786650592 33.0 27.0 35.0 2.0 35.0 90-91 27.352894459392523 33.0 26.0 35.0 2.0 35.0 92-93 26.867198878725013 32.5 25.0 34.0 2.0 35.0 94-95 26.29494785185814 32.0 25.0 34.0 2.0 35.0 96-97 25.448726758864076 32.0 22.0 34.0 2.0 35.0 98-99 24.164835078676496 31.5 9.5 34.0 2.0 35.0 100-101 18.318755805383706 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1526335760941251 1101 2 0.173498621267683 1101 3 0.16543081401665205 1101 4 0.12757292127148645 1101 5 0.06432671174476923 1101 6 0.06442906261265335 1101 7 0.07287676749833594 1101 8 0.10647364654211344 1101 9 0.03857923108117234 1101 10-11 0.15192002619096456 1101 12-13 0.13494565994421492 1101 14-15 0.2659861298386943 1101 16-17 0.15781975956797112 1101 18-19 0.18207895099565974 1101 20-21 0.23012908817590016 1101 22-23 0.20592882553872727 1101 24-25 0.2006803480620718 1101 26-27 0.19383380594708655 1101 28-29 0.3055940473702776 1101 30-31 0.16872044547532994 1101 32-33 0.245414135867982 1101 34-35 0.29773018028721054 1101 36-37 0.3675424884172074 1101 38-39 0.3187149222022754 1101 40-41 0.3925574389305808 1101 42-43 0.3751293786214447 1101 44-45 0.5034951605071782 1101 46-47 0.6085982082434853 1101 48-49 0.6212468731794658 1101 50-51 0.42729568434594256 1101 52-53 0.5253359275983129 1101 54-55 0.3707466080884032 1101 56-57 0.4412568717604515 1101 58-59 0.4786699145799531 1101 60-61 0.505854366737367 1101 62-63 0.36901265092262037 1101 64-65 0.319211445277368 1101 66-67 0.42271294222774713 1101 68-69 0.619374469286825 1101 70-71 0.6161461676964777 1101 72-73 0.507863702928077 1101 74-75 0.2879790193565448 1101 76-77 0.3192109621612609 1101 78-79 0.3543643271888577 1101 80-81 0.5018417052046154 1101 82-83 0.4638862691972072 1101 84-85 0.4423147298203425 1101 86-87 0.4954241455742334 1101 88-89 0.5874745859768176 1101 90-91 0.7252902367112668 1101 92-93 0.6467650473327176 1101 94-95 0.42948920307013694 1101 96-97 0.667964937346575 1101 98-99 0.4024569834885021 1101 100-101 -0.31962422682002867 1102 1 0.11290280740893621 1102 2 0.11738417668139078 1102 3 0.149803198657132 1102 4 0.08802230209620632 1102 5 0.15535186175115712 1102 6 0.17566358323055198 1102 7 0.2315842302272486 1102 8 0.14396744060427125 1102 9 0.19708242979884716 1102 10-11 0.20048450411201202 1102 12-13 0.2705861836544372 1102 14-15 0.2619363121159495 1102 16-17 0.3089148416281944 1102 18-19 0.23179170472985788 1102 20-21 0.3389060574086358 1102 22-23 0.18272358276244205 1102 24-25 0.23027877068998492 1102 26-27 0.18478022919913428 1102 28-29 0.2769453105445976 1102 30-31 0.20054094847545656 1102 32-33 0.18468105502766008 1102 34-35 0.27733924166571455 1102 36-37 0.3585683476736321 1102 38-39 0.3776004066909735 1102 40-41 0.36795284582733245 1102 42-43 0.38364818083997676 1102 44-45 0.2885626101858847 1102 46-47 0.4086152302444148 1102 48-49 0.3700035578610894 1102 50-51 0.42114152175745545 1102 52-53 0.3624453081393284 1102 54-55 0.3679542906848283 1102 56-57 0.3677331757166442 1102 58-59 0.2663431489019814 1102 60-61 0.22894896650756635 1102 62-63 -0.03438111051404036 1102 64-65 0.1351220514024476 1102 66-67 0.1144508885233293 1102 68-69 0.1285847902933952 1102 70-71 -0.12425200527542302 1102 72-73 -0.11592950813054514 1102 74-75 -0.05309790590268548 1102 76-77 -0.06677780331812144 1102 78-79 0.08854379581872962 1102 80-81 0.11258613404555717 1102 82-83 0.10870945316247926 1102 84-85 0.05902466073938939 1102 86-87 0.06294474928786897 1102 88-89 0.020707915054650528 1102 90-91 0.3233022088519242 1102 92-93 0.2677545929871421 1102 94-95 0.1781075839941515 1102 96-97 -0.015557056497137012 1102 98-99 -0.16434444068557497 1102 100-101 -0.6432344513685919 1103 1 0.0630776656708818 1103 2 0.09468880857389195 1103 3 0.07348024161877476 1103 4 0.06561246724469072 1103 5 0.09419508177633418 1103 6 0.05901543096808837 1103 7 0.0767182333705847 1103 8 0.040724539849705366 1103 9 0.12354650774037168 1103 10-11 0.10261695204538768 1103 12-13 0.10512871329805762 1103 14-15 0.014615140689024031 1103 16-17 0.12202838841204056 1103 18-19 0.17591523283472554 1103 20-21 0.229437791097439 1103 22-23 0.2094040735175824 1103 24-25 0.24919720033935988 1103 26-27 0.23229683044824156 1103 28-29 0.22195794148257875 1103 30-31 0.24552724781634083 1103 32-33 0.22874698276388727 1103 34-35 0.19847693123264065 1103 36-37 0.2790818961032002 1103 38-39 0.21545495753153432 1103 40-41 0.18598052691876887 1103 42-43 0.13503470399944462 1103 44-45 0.24688051484904605 1103 46-47 0.26771814564593654 1103 48-49 0.10909775092105889 1103 50-51 0.12679993511415688 1103 52-53 0.18776725305691855 1103 54-55 0.06992216613637225 1103 56-57 0.07272548195312112 1103 58-59 -0.06432740167524997 1103 60-61 0.10954587089918277 1103 62-63 0.12068689906408281 1103 64-65 0.015168872273527256 1103 66-67 -0.019451454165938742 1103 68-69 -0.14106968978099843 1103 70-71 -0.17738546532957145 1103 72-73 -0.2549673351154951 1103 74-75 -0.18080615180689108 1103 76-77 -0.19224992062538604 1103 78-79 -0.02437390971173059 1103 80-81 0.04649999680856354 1103 82-83 0.04750083285588502 1103 84-85 -0.21971257460748816 1103 86-87 -0.2228279596574687 1103 88-89 -0.10502093532427637 1103 90-91 0.04393260374359542 1103 92-93 0.04843213789485645 1103 94-95 -0.21525150493259204 1103 96-97 -0.3326326857811637 1103 98-99 -0.3559940329572555 1103 100-101 -0.8087873737644387 1104 1 0.1524994594098743 1104 2 0.15119556409155166 1104 3 0.1393889121325813 1104 4 0.07426892938418206 1104 5 0.09141253410585648 1104 6 0.12876647829362753 1104 7 0.09929775429605314 1104 8 0.08983551388428879 1104 9 0.12096163606702248 1104 10-11 0.21416535845136053 1104 12-13 0.1995785428918211 1104 14-15 0.05001899116341235 1104 16-17 0.09561562028915915 1104 18-19 0.1904805401415146 1104 20-21 0.21204468498829954 1104 22-23 0.16339399283560851 1104 24-25 0.2659270201207775 1104 26-27 0.2544063747058303 1104 28-29 0.1720006918940058 1104 30-31 0.16072106952847065 1104 32-33 0.19238015644804562 1104 34-35 0.26750753290598084 1104 36-37 0.2731607458035441 1104 38-39 0.19996239896108392 1104 40-41 0.3233435132394362 1104 42-43 0.2893601167969351 1104 44-45 0.2602196752579644 1104 46-47 0.3904670010701565 1104 48-49 0.307231816528855 1104 50-51 0.30365406054871613 1104 52-53 0.20864072692279478 1104 54-55 0.23408334908034334 1104 56-57 0.1774238990367749 1104 58-59 0.25998746046359855 1104 60-61 0.16562948863985127 1104 62-63 0.18079548590298344 1104 64-65 0.3569792509778651 1104 66-67 0.3850031625212438 1104 68-69 0.41278550818476845 1104 70-71 0.4484353068452762 1104 72-73 0.1505058289786021 1104 74-75 0.06350272448048244 1104 76-77 0.09494829600368959 1104 78-79 0.20083343176839463 1104 80-81 0.4279336955646329 1104 82-83 0.3527844185238145 1104 84-85 0.07138438427828575 1104 86-87 0.1792569267026458 1104 88-89 0.20929660218851254 1104 90-91 0.29331079762008727 1104 92-93 0.20706117149739356 1104 94-95 0.15448239459560753 1104 96-97 -0.21201499989229333 1104 98-99 -0.4897623328722247 1104 100-101 -1.392540867826618 1105 1 -0.0016007718005255356 1105 2 0.014456979437142081 1105 3 0.032318087851983535 1105 4 0.012600947113533323 1105 5 -0.11259667359518488 1105 6 0.053738881146330186 1105 7 0.02832944432041984 1105 8 -0.08489781547933006 1105 9 0.034880675274060025 1105 10-11 -0.019237546666865057 1105 12-13 0.0425596811513671 1105 14-15 -0.08115715879407048 1105 16-17 0.0190925096822383 1105 18-19 0.10278586510587218 1105 20-21 0.016698869448703135 1105 22-23 0.015630417812850794 1105 24-25 -0.02627511263359139 1105 26-27 -0.011304532389111444 1105 28-29 -0.09423849254348937 1105 30-31 -0.015729288432865474 1105 32-33 -0.05572761314770247 1105 34-35 0.02277541990348908 1105 36-37 0.14130083803323856 1105 38-39 0.04269084964782621 1105 40-41 0.07718599294680928 1105 42-43 0.06952968975239315 1105 44-45 0.10912077695541456 1105 46-47 0.10636562983928144 1105 48-49 -0.06605783468086912 1105 50-51 -0.05292411202901093 1105 52-53 0.04696661195275453 1105 54-55 -0.1542209824376073 1105 56-57 -0.3450208901281471 1105 58-59 -0.30702220477244424 1105 60-61 -0.4171270910340965 1105 62-63 -0.1978249061113715 1105 64-65 -0.2700304804200755 1105 66-67 -0.48073181732736714 1105 68-69 -0.508522670393333 1105 70-71 -0.5070185205444311 1105 72-73 -0.7016807201030915 1105 74-75 -0.6846648452961475 1105 76-77 -0.813264533603455 1105 78-79 -0.6391055313320955 1105 80-81 -0.4572453767131712 1105 82-83 -0.6319376531376655 1105 84-85 -0.6753217708652635 1105 86-87 -0.5851129295275399 1105 88-89 -0.6029670916712 1105 90-91 -0.4964802427351138 1105 92-93 -0.5567842244230654 1105 94-95 -0.5139841302810133 1105 96-97 -0.887155224954725 1105 98-99 -0.7822571048096059 1105 100-101 -1.3298513203384026 1106 1 0.01314840559368946 1106 2 0.04319950553891516 1106 3 0.05566120863682755 1106 4 0.05868448257192682 1106 5 0.07997918078475408 1106 6 0.07784125508437967 1106 7 0.10984593508365492 1106 8 0.11270222052649359 1106 9 0.044973556590854 1106 10-11 0.08867424298869508 1106 12-13 0.12410919355625794 1106 14-15 0.14917221618208032 1106 16-17 0.07977381219276225 1106 18-19 0.06566244635330065 1106 20-21 0.12718121045316622 1106 22-23 0.18132787891335767 1106 24-25 0.20915524986086353 1106 26-27 0.2559486638611972 1106 28-29 0.2605836944773827 1106 30-31 0.14594026620058997 1106 32-33 0.1733259034369823 1106 34-35 0.052985819127798095 1106 36-37 0.11979420973364796 1106 38-39 0.20632604807609312 1106 40-41 0.14850515616373627 1106 42-43 0.21953355450748546 1106 44-45 0.16511447398014667 1106 46-47 0.19188703422279474 1106 48-49 0.05269875130210977 1106 50-51 0.0327181271024628 1106 52-53 0.16999057245593008 1106 54-55 0.04372003259384627 1106 56-57 0.035848767826344385 1106 58-59 0.001575065242832352 1106 60-61 0.0294116049486135 1106 62-63 0.06278776030546851 1106 64-65 0.1502868306496552 1106 66-67 0.13602121112408128 1106 68-69 -0.03531643042823873 1106 70-71 0.007863721835398962 1106 72-73 0.03369949841700759 1106 74-75 0.038706747481946024 1106 76-77 0.007115836571593093 1106 78-79 0.08292489358549915 1106 80-81 0.26197135973107777 1106 82-83 0.15631681799261443 1106 84-85 0.04130680268787401 1106 86-87 -0.07514088295870991 1106 88-89 5.325174503987284E-4 1106 90-91 0.17350367205633077 1106 92-93 0.3083637076394332 1106 94-95 0.10946470867806468 1106 96-97 -0.23421732618783153 1106 98-99 -0.09512028171656794 1106 100-101 -0.7969615206501253 1107 1 0.05280529295095704 1107 2 0.035161339225993515 1107 3 0.05386681376725733 1107 4 0.02118952247272432 1107 5 -0.016768846997457842 1107 6 0.06065804613199077 1107 7 0.10290513585047734 1107 8 0.12402773345526441 1107 9 0.10669237196692904 1107 10-11 0.14990618492904417 1107 12-13 0.1365591749181263 1107 14-15 0.15323076271272384 1107 16-17 0.13530757572442553 1107 18-19 0.2223786371772576 1107 20-21 0.14618734201549444 1107 22-23 0.11439347940326883 1107 24-25 0.062293966949361845 1107 26-27 0.010110147988356744 1107 28-29 0.030873574356583333 1107 30-31 0.09818768158731217 1107 32-33 -0.040919566357842996 1107 34-35 -0.10467749267660764 1107 36-37 0.07004706060583743 1107 38-39 0.022831580555873643 1107 40-41 0.11339012280529914 1107 42-43 0.008559741071302085 1107 44-45 -0.07005680657306357 1107 46-47 0.03568933508014993 1107 48-49 -0.07227295868868566 1107 50-51 -0.1607206352500512 1107 52-53 -0.05326467094274534 1107 54-55 -0.2559436728933733 1107 56-57 -0.17583609953867807 1107 58-59 -0.261518454395663 1107 60-61 -0.36700203017017685 1107 62-63 -0.3815479617506554 1107 64-65 -0.42609595647253684 1107 66-67 -0.5250378527095947 1107 68-69 -0.6012147975003828 1107 70-71 -0.4157948562659506 1107 72-73 -0.5769398748095185 1107 74-75 -0.5194907938072042 1107 76-77 -0.5708614135063819 1107 78-79 -0.49551474361771497 1107 80-81 -0.4859473038774169 1107 82-83 -0.5368124183768295 1107 84-85 -0.5167497640175931 1107 86-87 -0.7206092989464175 1107 88-89 -0.8766458132583814 1107 90-91 -0.3216713361216179 1107 92-93 -0.23117336320825999 1107 94-95 -0.39953484248548676 1107 96-97 -0.5244664018890397 1107 98-99 -0.2682926995756958 1107 100-101 -0.8130649546293292 1108 1 -0.027221067312098057 1108 2 -0.004203626422999207 1108 3 -0.04938183607292501 1108 4 0.03498686991385114 1108 5 -0.04611507573473972 1108 6 0.01518062227365391 1108 7 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-0.6503684355034558 2316 92-93 -0.6682227334324118 2316 94-95 -0.5956887311716379 2316 96-97 -0.5640724049411574 2316 98-99 -0.17902169691062397 2316 100-101 1.245492725981979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 11.0 5 41.0 6 75.0 7 212.0 8 528.0 9 1050.0 10 1729.0 11 2846.0 12 4139.0 13 5612.0 14 7282.0 15 9914.0 16 12934.0 17 18047.0 18 18454.0 19 14620.0 20 14082.0 21 14311.0 22 15432.0 23 16457.0 24 18793.0 25 21491.0 26 24984.0 27 29952.0 28 36079.0 29 43695.0 30 52961.0 31 65644.0 32 82034.0 33 107603.0 34 151189.0 35 229499.0 36 367039.0 37 503773.0 38 265308.0 39 10415.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49978162085954 18.234171912320427 14.434652763271917 23.831393703548116 2 16.225834153504724 19.456417768094582 39.08915316804459 25.22859491035611 3 19.98781503574779 22.774122493480906 29.068057775732086 28.170004695039214 4 13.833255082679733 15.896904687014295 34.729638611117004 35.54020161918896 5 14.617206719163786 36.361695121310035 33.43698085918295 15.584117300343225 6 34.384659305263334 34.341214050435234 16.289341707536874 14.984784936764555 7 30.150453407962015 31.704336131601302 19.38442409210025 18.760786368336436 8 27.3593955667392 34.63144864558726 18.588527316549875 19.420628471123663 9 26.560270375224952 16.749713823984916 19.400058849410996 37.28995695137913 10-11 26.083940659869775 25.612568869030543 26.0087416280256 22.294748843074082 12-13 26.781393808636107 22.721891335208273 26.81905097484326 23.677663881312363 14-15 23.29493175760656 24.299277704779065 24.878276121457763 27.527514416156613 16-17 23.203416303403092 27.397164872198786 25.85451976912556 23.544899055272563 18-19 23.32087779707653 25.768593600541635 28.008376355819404 22.90215224656243 20-21 23.684005412669965 25.033160443971763 28.367629872256195 22.91520427110208 22-23 23.4212584734389 24.91073429859896 28.257587008116236 23.410420219845903 24-25 23.45604935236778 25.153643881029097 28.26630022582785 23.12400654077527 26-27 22.940289169529482 25.457637288880576 28.139086591046986 23.46298695054296 28-29 22.991912814280674 25.9039453567286 27.992362486711603 23.111779342279124 30-31 23.14587459172068 25.46705954951606 27.867640479908644 23.519425378854613 32-33 22.841209632396332 25.367030624530294 27.986844663115868 23.804915079957507 34-35 23.454710460373597 25.579050423957 27.807312609621686 23.158926506047713 36-37 23.693691470164964 25.1183715779963 27.779485981211405 23.408450970627335 38-39 23.206556023392622 25.510228794237417 27.745744201146106 23.53747098122386 40-41 23.593055323011612 25.37391913190154 27.85702721663579 23.175998328451065 42-43 23.36067530290685 25.504275877528706 27.46888944802308 23.666159371541365 44-45 23.29924442636455 25.701702923316333 27.723007683033586 23.276044967285532 46-47 23.858134923151535 25.882113645834508 27.05349147572067 23.20625995529328 48-49 23.627878917578645 26.054803669074833 26.739111585037378 23.57820582830914 50-51 23.604363097004676 26.2760808871566 26.859621244143554 23.259934771695168 52-53 24.000251816445182 25.847805092226622 26.82297119422796 23.32897189710024 54-55 23.517419148934206 26.08929060632419 26.939101928471214 23.454188316270386 56-57 23.42378823052474 26.309302295013314 26.75706083884912 23.50984863561282 58-59 23.516866877531463 25.909757985143532 26.73375414050458 23.839620996820425 60-61 23.176339684644432 26.140612839064886 27.08278227782422 23.60026519846646 62-63 23.248272659055804 26.107949527192158 27.255366967420695 23.38841084633134 64-65 23.03151644543329 26.209012097903983 26.999557925278992 23.75991353138373 66-67 22.991944695100564 26.202813072696063 26.95285971279844 23.852382519404934 68-69 23.513135995716155 25.818377964025014 27.07177631759492 23.59670972266391 70-71 23.284052998708862 26.026805593380626 26.72146991006306 23.967671497847448 72-73 23.229098543246476 26.084217381238105 26.763337739352455 23.92334633616296 74-75 23.20756039674465 26.553329280902627 26.486201288968136 23.75290903338459 76-77 23.3122037086023 26.134534356529755 26.699822219390786 23.853439715477162 78-79 23.362438918747376 26.283037845410202 26.648943003439022 23.7055802324034 80-81 23.51032602792733 26.256292850242907 26.467547675309106 23.76583344652066 82-83 23.349933540751373 26.38598203340396 26.510921731205777 23.753162694638892 84-85 23.143061257809308 26.509468944022036 26.49072107131755 23.856748726851105 86-87 23.411215352412885 26.360988456556434 26.313178381904844 23.914617809125836 88-89 23.161469434941736 26.430292436339585 26.12464648541894 24.283591643299733 90-91 23.07117973173483 26.38365406905585 26.30855056725955 24.236615631949764 92-93 23.231957997385905 26.097822848514422 26.447575597971817 24.222643556127856 94-95 22.973177397767596 25.88110389688255 26.645823398267538 24.499895307082316 96-97 23.168104541643338 25.448703698750048 26.727456201925428 24.655735557681187 98-99 22.81091511758036 25.8332966292254 26.446282135215167 24.90950611797907 100-101 23.405440220049492 24.663788294604906 27.072793388368765 24.857978096976833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 306.0 1 223.0 2 185.0 3 255.0 4 239.0 5 224.0 6 396.5 7 853.0 8 1444.5 9 1765.0 10 1590.0 11 1388.5 12 1487.0 13 1652.5 14 1798.0 15 1930.0 16 2127.5 17 2296.0 18 2464.5 19 2801.0 20 3182.0 21 3707.0 22 4496.0 23 5517.5 24 6782.5 25 8366.0 26 10555.5 27 13415.5 28 16062.0 29 18278.0 30 20678.5 31 24251.0 32 30014.0 33 32352.0 34 31647.5 35 33089.0 36 36149.5 37 39899.5 38 43691.0 39 47004.5 40 50385.5 41 54491.5 42 56985.0 43 58949.0 44 62332.5 45 68031.0 46 72109.5 47 75439.0 48 79953.0 49 81028.5 50 80720.0 51 81346.5 52 83233.0 53 87200.0 54 90128.0 55 86302.0 56 80427.5 57 74811.5 58 69928.0 59 63459.5 60 53161.5 61 45554.5 62 38970.5 63 29504.0 64 21186.0 65 16440.0 66 12635.0 67 9772.5 68 7532.0 69 5733.0 70 4310.5 71 3053.5 72 2268.0 73 1800.5 74 1376.5 75 1066.0 76 785.5 77 526.0 78 336.0 79 213.0 80 139.5 81 85.0 82 53.5 83 36.0 84 14.5 85 8.0 86 6.0 87 3.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4223229283024159 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.7628433305077E-4 16-17 0.002513552429014051 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.918034208295553E-5 26-27 0.0032975963059542136 28-29 4.612022805530369E-5 30-31 0.0 32-33 2.3060114027651846E-5 34-35 0.012844483513402076 36-37 0.027741317175265165 38-39 0.02160732684390978 40-41 0.009085684926894827 42-43 0.00975442823369673 44-45 0.004243060981087939 46-47 0.005326886340387576 48-49 0.003228415963871258 50-51 7.148635348572072E-4 52-53 0.0 54-55 0.0 56-57 6.918034208295553E-5 58-59 0.0014758472977697181 60-61 0.0026980333412352656 62-63 0.0013374866136038068 64-65 0.002790273797345873 66-67 0.002190710832626925 68-69 0.0046350829195580204 70-71 2.0754102624886658E-4 72-73 0.0 74-75 0.0 76-77 0.09652963731975062 78-79 0.015427216284499083 80-81 5.303826226359924E-4 82-83 0.0 84-85 0.0 86-87 0.10997368379787165 88-89 0.4248595408454576 90-91 0.4254821639242042 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.5366125430417025E-4 100-101 2.9978148235947397E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2168246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74233554656752 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91728336006193 38.76395213853604 2 13.475453438032995 13.945028668657 3 4.649590876767648 7.217420738999119 4 2.1708813229322748 4.493058793717526 5 1.2404408798790258 3.209165411619 6 0.7285959929858389 2.2619555008174697 7 0.5125593877463501 1.85647138798204 8 0.3611780203139441 1.4950555455303258 9 0.2769399746211754 1.2896568983796075 >10 1.5171960479226132 14.109856091067297 >50 0.09879298115469846 3.4758208280359937 >100 0.045483914018479885 4.190754683104387 >500 0.0029353256759390126 1.0799171335332318 >1k 0.002490579361402747 2.0882662845125552 >5k 1.7789852581448192E-4 0.5236198955083274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6038 0.27847393699792367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 0.24623589758726638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3237 0.14929117821501803 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2644 0.12194188297822296 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2640 0.12175740206600175 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2597 0.11977423225962368 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2417 0.111472591209669 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2169 0.10003477465195369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8448091222121474E-4 2 4.6120228055303685E-5 0.0 0.0 0.0 1.8448091222121474E-4 3 4.6120228055303685E-5 0.0 0.0 0.0 2.3060114027651844E-4 4 4.6120228055303685E-5 0.0 0.0 0.0 2.3060114027651844E-4 5 4.6120228055303685E-5 0.0 0.0 0.0 2.7672136833182214E-4 6 4.6120228055303685E-5 0.0 0.0 0.0 3.228415963871258E-4 7 9.224045611060737E-5 0.0 0.0 0.0 3.228415963871258E-4 8 9.224045611060737E-5 0.0 0.0 0.0 3.228415963871258E-4 9 9.224045611060737E-5 0.0 0.0 0.0 3.228415963871258E-4 10-11 2.3060114027651844E-4 0.0 0.0 0.0 3.9202193847008137E-4 12-13 2.3060114027651844E-4 0.0 0.0 2.3060114027651843E-5 0.001314426499576155 14-15 2.7672136833182214E-4 0.0 0.0 4.6120228055303685E-5 0.0016603282099909326 16-17 2.7672136833182214E-4 0.0 0.0 1.1530057013825922E-4 0.0019139894642951029 18-19 2.7672136833182214E-4 0.0 0.0 2.3060114027651844E-4 0.0020292900344333624 20-21 2.7672136833182214E-4 0.0 0.0 2.7672136833182214E-4 0.0023290715167928362 22-23 2.7672136833182214E-4 0.0 0.0 3.228415963871258E-4 0.0023982518588757918 24-25 3.228415963871258E-4 0.0 0.0 4.3814216652538504E-4 0.0024443720869310953 26-27 3.228415963871258E-4 0.0 0.0 6.226230787465998E-4 0.0028363940254011767 28-29 4.3814216652538504E-4 0.0 0.0 0.0020062299204057104 0.003090055279705347 30-31 5.073225086083406E-4 0.0 0.0 0.006157050445383042 0.003136175507760651 32-33 5.073225086083406E-4 0.0 0.0 0.013467106592148677 0.00325147607789891 34-35 5.534427366636443E-4 0.0 0.0 0.025227764746251116 0.0035281974462307323 36-37 5.534427366636443E-4 0.0 0.0 0.044782741441699886 0.0035743176742860355 38-39 5.995629647189479E-4 0.0 0.0 0.07683629994013594 0.0037587985865072506 40-41 6.226230787465998E-4 0.0 0.0 0.11345576101604707 0.003827978928590206 42-43 7.37923648884859E-4 0.0 0.0 0.15152800927570026 0.003943279498728466 44-45 9.224045611060738E-4 0.0 0.0 0.20106113420709643 0.004081640182894376 46-47 0.0010146450172166812 0.0 0.0 0.26115579136315714 0.004289181209143243 48-49 0.0011068854733272885 0.0 0.0 0.3260008320089141 0.004542842463447413 50-51 0.0011068854733272885 0.0 0.0 0.39663396127561173 0.0046120228055303684 52-53 0.0011068854733272885 0.0 0.0 0.46740545122647525 0.005119345314138709 54-55 0.0011068854733272885 0.0 0.0 0.5494763970508882 0.005303826226359924 56-57 0.0011068854733272885 0.0 0.0 0.6488193682820123 0.005373006568442879 58-59 0.0011068854733272885 0.0 0.0 0.7558182973703168 0.005396066682470531 60-61 0.0011068854733272885 0.0 0.0 0.8829025857767061 0.005419126796498183 62-63 0.0011530057013825921 0.0 0.0 1.0087416280256023 0.005580547594691746 64-65 0.0011530057013825921 0.0 0.0 1.144611819876527 0.0058111487349682646 66-67 0.0011991259294378959 0.0 0.0 1.307739066508136 0.00588032907705122 68-69 0.0011991259294378959 0.0 0.0 1.497569925183766 0.005926449305106524 70-71 0.0012221860434655477 0.0 0.0 1.7063331374760982 0.00599562964718948 72-73 0.0012683062715208515 0.0 0.0 1.922613946941445 0.006318471243576605 74-75 0.0012913663855485032 0.0 0.0 2.143437598870239 0.00664131283996373 76-77 0.0012913663855485032 0.0 0.0 2.3888433323525096 0.006733553296074338 78-79 0.0012913663855485032 0.0 0.0 2.667478690148627 0.0068719139802402495 80-81 0.001614207981935629 0.0 0.0 2.976322797320968 0.006941094322323205 82-83 0.0017064484380462364 0.0 0.0 3.306981772363468 0.007425356716903894 84-85 0.0017064484380462364 0.0 0.0 3.6437747377373233 0.007448416830931545 86-87 0.0017064484380462364 0.0 0.0 4.000630002315235 0.007725138199263367 88-89 0.0017064484380462364 0.0 0.0 4.387694016269371 0.007978799453567538 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4430 0.0 45.620136 1 GTATCAA 7485 0.0 37.7622 1 ATCAACG 9355 0.0 29.571402 3 TCAACGC 9400 0.0 29.429834 4 TATCAAC 9435 0.0 29.370955 2 CAACGCA 9640 0.0 28.647917 5 AACGCAG 9840 0.0 28.113865 6 ACGCAGA 11245 0.0 24.516802 7 CGCAGAG 11370 0.0 24.247269 8 GTAAGGT 1080 0.0 22.84687 4 GTACATG 7760 0.0 22.173788 1 TACATGG 7585 0.0 21.895739 2 ACATGGG 7555 0.0 21.417416 3 GCAGAGT 12875 0.0 21.302853 9 GAGTACT 7460 0.0 20.864702 12-13 AGAGTAC 11010 0.0 20.7317 10-11 TAAGGTG 1265 0.0 20.63095 5 GTACTTT 7585 0.0 20.364445 14-15 AGTACTT 7995 0.0 19.794958 12-13 TGGTATC 2485 0.0 19.6679 2 >>END_MODULE