##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 873508 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.213975143902516 33.0 31.0 34.0 31.0 34.0 2 32.287499370354936 34.0 31.0 34.0 31.0 34.0 3 32.3005410368308 34.0 31.0 34.0 30.0 34.0 4 35.768836690677134 37.0 35.0 37.0 35.0 37.0 5 35.85016737110593 37.0 35.0 37.0 35.0 37.0 6 35.973898350100974 37.0 37.0 37.0 35.0 37.0 7 35.92681120264497 37.0 37.0 37.0 35.0 37.0 8 35.92406480535954 37.0 37.0 37.0 35.0 37.0 9 37.5961055880427 39.0 38.0 39.0 35.0 39.0 10-11 37.53453374210655 39.0 38.0 39.0 35.0 39.0 12-13 37.477660193152204 39.0 38.0 39.0 35.0 39.0 14-15 38.71581370748751 40.0 38.0 41.0 35.0 41.0 16-17 38.69418883398893 40.0 38.0 41.0 35.0 41.0 18-19 38.75506291871397 40.0 38.5 41.0 35.0 41.0 20-21 38.71714855502182 40.0 38.5 41.0 35.0 41.0 22-23 38.62077107479267 40.0 38.0 41.0 35.0 41.0 24-25 38.60432417333327 40.5 38.0 41.0 35.0 41.0 26-27 38.496776217275624 40.0 38.0 41.0 34.5 41.0 28-29 38.374908987668114 40.0 38.0 41.0 34.0 41.0 30-31 38.1122720112466 40.0 37.5 41.0 34.0 41.0 32-33 37.78861269730787 40.0 36.5 41.0 34.0 41.0 34-35 37.44330561368642 40.0 36.0 41.0 33.0 41.0 36-37 37.14789961854957 40.0 35.0 41.0 33.0 41.0 38-39 36.91194356548537 40.0 35.0 41.0 32.5 41.0 40-41 36.78506149915055 40.0 35.0 41.0 32.0 41.0 42-43 36.509813876919274 40.0 35.0 41.0 31.0 41.0 44-45 36.11412145051906 40.0 35.0 41.0 30.0 41.0 46-47 35.58434095623623 39.0 35.0 41.0 28.0 41.0 48-49 35.32041950388548 39.5 35.0 41.0 24.5 41.0 50-51 34.31244934219263 38.5 34.0 40.0 23.0 40.5 52-53 34.53033629915238 38.5 35.0 40.0 23.0 40.5 54-55 35.060647984906836 39.0 35.0 41.0 23.5 41.0 56-57 34.97436772187547 39.5 35.0 41.0 23.0 41.0 58-59 34.84075932905022 39.0 35.0 41.0 22.0 41.0 60-61 34.56972632191119 39.0 35.0 41.0 21.0 41.0 62-63 34.270837817169394 39.0 35.0 41.0 18.5 41.0 64-65 33.962443389184756 38.0 35.0 40.0 14.0 41.0 66-67 33.57079671851889 37.5 34.5 40.0 8.5 41.0 68-69 33.14874734976669 37.0 34.0 39.5 4.5 41.0 70-71 32.73827200208813 36.5 34.0 39.0 2.0 41.0 72-73 32.27672442610715 36.0 33.5 39.0 2.0 40.5 74-75 31.799580541906884 35.5 33.0 38.5 2.0 40.0 76-77 31.35835046731112 35.0 32.5 37.0 2.0 39.5 78-79 30.911014552814628 35.0 32.0 37.0 2.0 39.0 80-81 30.427073363953163 35.0 31.5 36.0 2.0 39.0 82-83 29.951187625070403 35.0 31.0 36.0 2.0 37.5 84-85 29.510022804599387 35.0 31.0 35.5 2.0 37.0 86-87 29.159639636958104 35.0 30.5 35.0 2.0 37.0 88-89 28.729179927373302 34.0 30.0 35.0 2.0 36.0 90-91 28.325243157475377 34.0 29.0 35.0 2.0 36.0 92-93 27.921086011805272 34.0 29.0 35.0 2.0 36.0 94-95 27.472841691203744 34.0 28.5 35.0 2.0 35.0 96-97 26.950590607069426 34.0 26.5 35.0 2.0 35.0 98-99 26.39101817041172 34.0 25.0 35.0 2.0 35.0 100-101 24.104821020528718 31.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1779673186065196 1101 2 0.25923784274299067 1101 3 0.2864285038856309 1101 4 0.3188953285932854 1101 5 0.24427982935446124 1101 6 0.3791138069564255 1101 7 0.2664767265763359 1101 8 0.4413901384114567 1101 9 0.37575140209785474 1101 10-11 0.40684938121194136 1101 12-13 0.3745774429630657 1101 14-15 0.45849166047806733 1101 16-17 0.32082484568242364 1101 18-19 0.3782751638181452 1101 20-21 0.5109082452468243 1101 22-23 0.4691924406707173 1101 24-25 0.4541836128400192 1101 26-27 0.3895740808923236 1101 28-29 0.42147355537644415 1101 30-31 0.5306733270956272 1101 32-33 0.4576623748752482 1101 34-35 0.4419772588770101 1101 36-37 0.42995405783108254 1101 38-39 0.6325772463886707 1101 40-41 0.43030285157006176 1101 42-43 0.3016352894996359 1101 44-45 0.3666872929917133 1101 46-47 0.5918224463594512 1101 48-49 0.6765462373606965 1101 50-51 0.469472662555404 1101 52-53 0.5724542382632549 1101 54-55 0.6612021163440645 1101 56-57 0.6430075907512887 1101 58-59 0.5717288596019969 1101 60-61 0.5298109766393182 1101 62-63 0.6891167658683344 1101 64-65 0.545096607669322 1101 66-67 0.5662866909741737 1101 68-69 0.6017978196936582 1101 70-71 0.6365064463637324 1101 72-73 0.7269982043901138 1101 74-75 0.5606679826581349 1101 76-77 0.6386685640023586 1101 78-79 0.42345094096527447 1101 80-81 0.34736543156641275 1101 82-83 0.43033471021343317 1101 84-85 0.2740249751249344 1101 86-87 0.37397310773909354 1101 88-89 0.6869000916177619 1101 90-91 0.2872156812176634 1101 92-93 0.4039512484001655 1101 94-95 0.17102650149574572 1101 96-97 0.41470261144528564 1101 98-99 0.37056369571728 1101 100-101 0.44499188647543875 1102 1 0.16093985329209204 1102 2 0.24694666407687293 1102 3 0.3464833683241295 1102 4 0.2381725300656541 1102 5 0.28075806192526187 1102 6 0.22107333523992168 1102 7 0.2990515676275862 1102 8 0.2670282316883572 1102 9 0.3232569455389438 1102 10-11 0.1978184445818414 1102 12-13 0.20020726244729303 1102 14-15 0.45931260457296474 1102 16-17 0.39641605413419256 1102 18-19 0.6357234158415466 1102 20-21 0.4255374138593737 1102 22-23 0.401115674583707 1102 24-25 0.3575861641099536 1102 26-27 0.37782989185271276 1102 28-29 0.29140861529709383 1102 30-31 0.4577591502276803 1102 32-33 0.6313200899111138 1102 34-35 0.5977994348477154 1102 36-37 0.5813800919632328 1102 38-39 0.4792353695983209 1102 40-41 0.5949651153532756 1102 42-43 0.4725764426089327 1102 44-45 0.7704869863098054 1102 46-47 1.0357500341260035 1102 48-49 1.0365122964245401 1102 50-51 0.8110396453422339 1102 52-53 0.9169869160670601 1102 54-55 1.1422863876803362 1102 56-57 1.1442091293090044 1102 58-59 0.8921654952204889 1102 60-61 0.8575303884278256 1102 62-63 0.844119836364726 1102 64-65 1.0693590450183237 1102 66-67 1.0277308171329693 1102 68-69 1.1197041456516743 1102 70-71 1.07266862005455 1102 72-73 0.9008728766559884 1102 74-75 0.8123264184332832 1102 76-77 0.7518053242793314 1102 78-79 0.6241281049297847 1102 80-81 0.6204143793238437 1102 82-83 0.4978157632282212 1102 84-85 0.5900765006284381 1102 86-87 0.612281319386387 1102 88-89 0.675891350815462 1102 90-91 0.862524661408365 1102 92-93 0.8888957036720413 1102 94-95 0.47028222787958285 1102 96-97 0.6270210016961819 1102 98-99 0.2618718007245775 1102 100-101 0.26916459913949 1103 1 0.14081406712857358 1103 2 0.34931724946633125 1103 3 0.2794987805848308 1103 4 0.16674050297324783 1103 5 0.18675018301172486 1103 6 0.07776004475682186 1103 7 0.199099946921244 1103 8 0.1226298627616913 1103 9 0.05300329981360363 1103 10-11 0.0813159219472368 1103 12-13 0.19103247497061915 1103 14-15 0.17660129072957886 1103 16-17 0.19884020231306465 1103 18-19 0.32161566824323273 1103 20-21 0.25995064214052377 1103 22-23 0.23228230669373318 1103 24-25 0.18684181412032075 1103 26-27 0.25316854690834845 1103 28-29 0.14684610233835116 1103 30-31 0.04750965129893814 1103 32-33 0.06174202646512583 1103 34-35 -0.06861496835914238 1103 36-37 -0.3033320512394724 1103 38-39 -0.2078991939513415 1103 40-41 -0.26343905908863263 1103 42-43 -0.2304299849257987 1103 44-45 -0.20188670917710283 1103 46-47 -0.3639424117928556 1103 48-49 -0.23385884187112538 1103 50-51 -0.22791950871125977 1103 52-53 -0.29303969254445406 1103 54-55 -0.28461941092357534 1103 56-57 -0.42801770046499854 1103 58-59 -0.5284268055604855 1103 60-61 -0.5380826463361572 1103 62-63 -0.5537497468822181 1103 64-65 -0.546053215676956 1103 66-67 -0.47204629254979835 1103 68-69 -0.7003438880756079 1103 70-71 -0.6419522936229711 1103 72-73 -0.793556593245178 1103 74-75 -0.8344408080509282 1103 76-77 -0.9118255417100265 1103 78-79 -1.078840310302784 1103 80-81 -1.0374196105856157 1103 82-83 -0.9549453121743632 1103 84-85 -0.8440689137056552 1103 86-87 -0.865804588416502 1103 88-89 -0.7403675475189075 1103 90-91 -1.0742764305814063 1103 92-93 -1.1494742596979215 1103 94-95 -1.1305056634743451 1103 96-97 -1.0387316418866526 1103 98-99 -1.1998809119715297 1103 100-101 -1.1294099395293884 1104 1 0.0837920705126649 1104 2 0.1687692864310577 1104 3 0.29373801005248623 1104 4 0.2535685649282158 1104 5 0.20024100690863378 1104 6 0.1482012786498359 1104 7 0.2840146937649237 1104 8 0.2540368652346814 1104 9 0.40532010314563394 1104 10-11 0.4638780721489866 1104 12-13 0.33755726997257085 1104 14-15 0.4501728122706439 1104 16-17 0.44707656831592146 1104 18-19 0.3960364674102266 1104 20-21 0.38168043519011263 1104 22-23 0.42770727090042726 1104 24-25 0.4261038211488213 1104 26-27 0.35061746009329653 1104 28-29 0.3404033065554515 1104 30-31 0.20733328789922467 1104 32-33 0.40287830135774527 1104 34-35 0.27356256645798993 1104 36-37 0.4210985097393163 1104 38-39 0.6481043203009023 1104 40-41 0.6343873108189584 1104 42-43 0.627369702696221 1104 44-45 0.49127351469378766 1104 46-47 0.4891976376669902 1104 48-49 0.7184547075236409 1104 50-51 0.7186052862906962 1104 52-53 0.6095211560823799 1104 54-55 0.669457772468327 1104 56-57 0.6870057278833102 1104 58-59 0.8034715414393858 1104 60-61 0.8678297576726948 1104 62-63 0.873856656866316 1104 64-65 0.8388289917940881 1104 66-67 0.7340157925731958 1104 68-69 0.8104494218293823 1104 70-71 0.8888722540797502 1104 72-73 0.7162572815433137 1104 74-75 0.6418412432311236 1104 76-77 0.793647580768166 1104 78-79 0.6499928277943141 1104 80-81 0.6472283134024082 1104 82-83 0.5489756822288641 1104 84-85 0.35426507041513133 1104 86-87 0.47533494041338287 1104 88-89 0.23515633638061217 1104 90-91 0.32731149080347066 1104 92-93 0.21494719025652032 1104 94-95 0.18697774144560597 1104 96-97 0.2742222823616878 1104 98-99 0.37532734001337076 1104 100-101 0.32901450404733623 1105 1 0.0575227153233655 1105 2 0.08077110207887017 1105 3 0.12538782017664118 1105 4 0.151294529970599 1105 5 0.018430090006489763 1105 6 0.22403014745010097 1105 7 0.12365591152758526 1105 8 0.19342363657813166 1105 9 0.3875056572378739 1105 10-11 0.2024471626628639 1105 12-13 0.2997323393379858 1105 14-15 0.40771606176878805 1105 16-17 0.20648245510645324 1105 18-19 0.3814409604992548 1105 20-21 0.3492017562656926 1105 22-23 0.29555815762702053 1105 24-25 0.18645429232221744 1105 26-27 0.24577818328372558 1105 28-29 0.3422685010982818 1105 30-31 0.25503080353041696 1105 32-33 0.25371548122195975 1105 34-35 0.10784954110284417 1105 36-37 0.19161724799194246 1105 38-39 0.22478826971849486 1105 40-41 0.23480094607832314 1105 42-43 0.1669639309819715 1105 44-45 0.2494583143971454 1105 46-47 0.31188268670226904 1105 48-49 0.1541957987024034 1105 50-51 0.07498690477450509 1105 52-53 0.11502883422684818 1105 54-55 0.02431942521929642 1105 56-57 0.05308092383261709 1105 58-59 0.18355407669746882 1105 60-61 0.09967327951800797 1105 62-63 0.15836515333091938 1105 64-65 0.1806502655605371 1105 66-67 0.21817362607061597 1105 68-69 0.34878580255282543 1105 70-71 0.38812585660630816 1105 72-73 0.5578707787111625 1105 74-75 0.4680377167827565 1105 76-77 0.3700981810827102 1105 78-79 0.43997064364670635 1105 80-81 0.16205077629031095 1105 82-83 0.161278933772806 1105 84-85 0.383894013375663 1105 86-87 0.3165712431406895 1105 88-89 0.17209773698667874 1105 90-91 0.12854440517393684 1105 92-93 0.22399825854789768 1105 94-95 0.3464271014936813 1105 96-97 0.5572235993456722 1105 98-99 0.6132011780627025 1105 100-101 0.4959582061788055 1106 1 0.11897498666880324 1106 2 0.2765497870436917 1106 3 0.17097913930656716 1106 4 0.18235879272947386 1106 5 0.11842884917314933 1106 6 0.11104916942525733 1106 7 0.1863552203834189 1106 8 0.1216383255632536 1106 9 0.25805571865912214 1106 10-11 0.2362862389865228 1106 12-13 0.22163118660380832 1106 14-15 0.34814985710573154 1106 16-17 0.3594818595064453 1106 18-19 0.33217522363037233 1106 20-21 0.3624042230423754 1106 22-23 0.4730205913975354 1106 24-25 0.40932192022111735 1106 26-27 0.41346285030609664 1106 28-29 0.24178736796927325 1106 30-31 0.4616280042103966 1106 32-33 0.536202049201016 1106 34-35 0.4960938860048856 1106 36-37 0.44470382093432903 1106 38-39 0.500996220978557 1106 40-41 0.3399460615378729 1106 42-43 0.37891067441486115 1106 44-45 0.16860716476504933 1106 46-47 0.15341896246506792 1106 48-49 0.0631760198189113 1106 50-51 -0.08081833402387417 1106 52-53 -0.12632565945111196 1106 54-55 -0.2196547778554887 1106 56-57 -0.17733562392084679 1106 58-59 -0.2129790349403038 1106 60-61 -0.27707784653825485 1106 62-63 -0.18390321410465305 1106 64-65 -0.38269272559232803 1106 66-67 -0.4467905138457482 1106 68-69 -0.37150089199123215 1106 70-71 -0.49089444342718735 1106 72-73 -0.5725795817509223 1106 74-75 -0.4899321557836558 1106 76-77 -0.5440283963266737 1106 78-79 -0.38514320281257497 1106 80-81 -0.3249685558931823 1106 82-83 -0.3578283486780869 1106 84-85 -0.35989561573925144 1106 86-87 -0.4609132154216802 1106 88-89 -0.30397434302225435 1106 90-91 -0.23153612491006825 1106 92-93 -0.3684934588895352 1106 94-95 -0.3734764857555142 1106 96-97 -0.3048267536714171 1106 98-99 -0.08589518505477045 1106 100-101 0.33481893504431426 1107 1 0.041206223991316904 1107 2 0.12093112578608611 1107 3 0.11500405961951543 1107 4 0.24989284083147112 1107 5 0.2864786897990541 1107 6 0.26798628910071187 1107 7 0.2761436874943968 1107 8 0.1685725475279014 1107 9 0.3270907809599066 1107 10-11 0.2611877168190375 1107 12-13 0.21460713560410483 1107 14-15 0.23780792723032818 1107 16-17 0.20453401830098983 1107 18-19 0.13750556266253966 1107 20-21 0.11327643399572196 1107 22-23 0.3501706928174215 1107 24-25 0.2973848005456645 1107 26-27 0.4421806132733579 1107 28-29 0.3742895412523737 1107 30-31 0.4196966799565516 1107 32-33 0.33131216221173787 1107 34-35 0.3026369170103962 1107 36-37 0.3848429110531342 1107 38-39 0.3124175481301066 1107 40-41 0.22892896504409777 1107 42-43 0.3156333634904911 1107 44-45 0.38439534210495196 1107 46-47 0.526675989413782 1107 48-49 0.4844669499840819 1107 50-51 0.2997727989810528 1107 52-53 0.4105274266108978 1107 54-55 0.4194831531789873 1107 56-57 0.3213488153721116 1107 58-59 0.4528250798090454 1107 60-61 0.2835538544179954 1107 62-63 0.27401043411325077 1107 64-65 0.1339618672793037 1107 66-67 0.16580438618323257 1107 68-69 0.16500301991835897 1107 70-71 0.23500094921262615 1107 72-73 0.2413453675391395 1107 74-75 0.18184124323112272 1107 76-77 0.21021819886312443 1107 78-79 0.08842967461425744 1107 80-81 0.06540090223489514 1107 82-83 0.15553734242594075 1107 84-85 0.09418146336825117 1107 86-87 0.031880177797678044 1107 88-89 0.1419703107013035 1107 90-91 -0.00757205652409354 1107 92-93 -0.03278496859686442 1107 94-95 -0.10059616121190373 1107 96-97 -0.052917162248643734 1107 98-99 -0.053485738517533576 1107 100-101 0.1849565763077159 1108 1 0.12784892807647452 1108 2 0.17917955324167423 1108 3 0.2578560676377677 1108 4 0.03716594513782212 1108 5 0.0783166658825536 1108 6 0.2559395620667715 1108 7 0.22066432065953023 1108 8 0.1143982197474358 1108 9 0.1309146559858263 1108 10-11 0.23555904466861932 1108 12-13 0.2014274853656346 1108 14-15 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Count 2 3309.0 3 569.0 4 463.0 5 514.0 6 642.0 7 665.0 8 901.0 9 1039.0 10 1482.0 11 2187.0 12 3589.0 13 6023.0 14 5511.0 15 6466.0 16 9184.0 17 13648.0 18 12434.0 19 5743.0 20 5193.0 21 4831.0 22 5220.0 23 5692.0 24 6403.0 25 7090.0 26 8070.0 27 9120.0 28 10790.0 29 12503.0 30 14768.0 31 17873.0 32 22323.0 33 28915.0 34 39778.0 35 70422.0 36 91914.0 37 161495.0 38 225381.0 39 51348.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37544926480808 23.281134955759835 15.578878207742115 18.76453757168997 2 14.256403908137674 24.2979427717334 40.91933391102614 20.52631940910279 3 18.068348011865083 30.842452972918455 32.57923427120058 18.509964744015882 4 10.262232471986357 20.120307370660406 42.39505199259083 27.222408164762413 5 9.566910131874758 41.930280521904415 35.975796401789815 12.527012944431007 6 23.705179739385912 42.48167772038299 19.343698006664315 14.469444533566783 7 23.078342302368007 37.9189624033957 23.82206932590141 15.180625968334876 8 23.96735225255476 34.98321657324714 21.580083132111756 19.46934804208635 9 25.789144995044033 18.590556730091613 24.630078436090916 30.99021983877344 10-11 22.1999667696045 29.551848011046246 28.85160336658283 19.39658185276643 12-13 24.528112702912683 29.296661036410264 28.838983070269723 17.336243190407327 14-15 21.874004469145707 29.50575832235146 29.94803185698734 18.6722053515155 16-17 16.977935695956837 32.87903848147998 30.78077260870736 19.362253213855833 18-19 16.707664292758004 31.319264071124437 34.90018181057664 17.07288982554092 20-21 18.22512998098063 29.408565765247445 34.73931886699793 17.626985386774003 22-23 18.013584561372944 29.612613056629467 34.60504251765982 17.768759864337774 24-25 18.12952411303168 30.5825885449415 34.700538035936695 16.58734930609012 26-27 17.49825470827549 29.633964465975122 36.341788302132066 16.525992523617322 28-29 16.369037384484532 30.74476669945002 35.93983910939323 16.946356806672213 30-31 17.076399546579342 30.055336575436197 35.351968356831506 17.516295521152944 32-33 17.23860895536415 30.583063096929248 35.21661221825742 16.961715729449185 34-35 18.01147284631451 30.16138822703808 35.151944290826144 16.67519463582126 36-37 17.914184600713547 29.492838938963178 35.3750836142523 17.217892846070978 38-39 16.845124425913436 31.464630172385537 34.75214008430957 16.938105317391454 40-41 16.750812618788366 31.174149351687152 35.106223964291914 16.968814065232564 42-43 16.632382481538585 31.14940102835303 33.96158599388003 18.256630496228357 44-45 17.356982234568115 30.44698263715155 33.93720848645882 18.258826641821518 46-47 18.28961285680516 31.445326541892342 33.23946788875794 17.02559271254455 48-49 17.483138957994797 31.39308877033032 33.1232258157293 18.000546455945585 50-51 16.439125140841465 31.460086722107928 33.798673343851036 18.30211479319958 52-53 17.269211899019727 32.627400139307724 32.08753955056865 18.015848411103896 54-55 17.237567789835694 31.474899788938536 32.39114807082923 18.89638435039653 56-57 17.866132940206462 31.50134140746013 31.981902211223666 18.65062344110974 58-59 18.53764677138874 30.833244707606962 32.328020580330836 18.30108794067346 60-61 17.989927860773708 31.906121318480263 32.74697945003177 17.356971370714255 62-63 17.33231820395705 31.58187097007918 33.10801524764192 17.97779557832185 64-65 17.230476140077 32.074816177845165 32.44030345682861 18.254404225249225 66-67 17.668979593245535 31.356128181616317 31.366425326143354 19.60846689899479 68-69 17.32152452797189 31.16892904936202 32.221089464703034 19.288456957963064 70-71 18.302768602030284 31.287055350179894 32.17799454402576 18.23218150376407 72-73 18.313003511819677 30.264234949652412 33.00633047920088 18.41643105932703 74-75 17.101276359760064 32.489617747884 31.824946513927664 18.58415937842827 76-77 17.351889683350358 31.215088939808037 32.2517806366121 19.181240740229505 78-79 17.589719085003626 31.05429520541762 32.30209071875897 19.05389499081979 80-81 18.043114864674482 30.88148616268044 31.774800765254636 19.300598207390447 82-83 17.913778210255472 30.740077454914776 32.49799576493917 18.84814856989058 84-85 17.517345215803008 30.89674075573611 32.06357458672838 19.5223394417325 86-87 17.279556182066116 31.059088496150355 32.29084890072127 19.37050642106226 88-89 17.855891808131073 30.491084190709337 31.754787491350505 19.898236509809088 90-91 18.060459066415 29.705735655109372 32.384610649572245 19.849194628903387 92-93 18.881651079554672 30.285227467524773 32.28689336528605 18.546228087634507 94-95 18.466898152942015 29.201326328917602 32.587100737948795 19.744674780191588 96-97 18.317084858515447 29.145201939577188 32.81231213869507 19.725401063212292 98-99 18.437368055931056 29.565307927976225 31.827584263005733 20.169739753086986 100-101 18.418909405469076 28.513937266197644 32.71122159286986 20.355931735463418 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6127.0 1 3821.5 2 2041.5 3 2509.5 4 2022.5 5 1866.0 6 3199.0 7 6111.5 8 8874.5 9 8957.0 10 6253.0 11 3967.0 12 3474.0 13 3372.0 14 3193.0 15 2923.0 16 2803.5 17 2740.0 18 2625.0 19 2629.0 20 2855.5 21 3235.5 22 3652.0 23 4123.0 24 4604.5 25 5891.0 26 8752.0 27 11432.5 28 11777.0 29 12557.5 30 15496.0 31 18613.0 32 23417.0 33 29668.0 34 36061.0 35 39990.5 36 44689.5 37 49242.5 38 46760.0 39 43166.5 40 39291.5 41 34818.5 42 31410.0 43 29384.0 44 28182.5 45 27057.5 46 25448.0 47 26249.5 48 34823.0 49 32104.5 50 18569.5 51 10894.5 52 6929.0 53 5432.5 54 4582.5 55 3783.0 56 3212.5 57 2835.0 58 2545.5 59 2428.5 60 2302.5 61 2019.5 62 1569.5 63 986.5 64 554.0 65 446.5 66 436.0 67 397.5 68 341.5 69 278.0 70 226.0 71 212.5 72 203.0 73 241.0 74 241.5 75 157.0 76 112.0 77 84.5 78 66.0 79 53.5 80 38.5 81 25.5 82 21.0 83 20.5 84 12.0 85 8.0 86 8.0 87 8.5 88 5.5 89 3.0 90 1.5 91 2.0 92 3.0 93 3.0 94 2.5 95 1.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33416980725992207 2 0.12272354689367469 3 0.11814431006928386 4 0.12318147057611378 5 0.14207082247672603 6 0.09158473648781694 7 0.10028528645415954 8 0.10543692788159925 9 0.09421779766184167 10-11 0.09307298845574397 12-13 0.09639293515342734 14-15 0.09822462988318366 16-17 0.08740618288556029 18-19 0.10263214532665987 20-21 0.11179061897544157 22-23 0.11986152387843042 24-25 0.1337709557325176 26-27 0.13291234882794434 28-29 0.1275317455592851 30-31 0.11780086730745455 32-33 0.11098925253117316 34-35 0.3255264977538843 36-37 0.4795033359740266 38-39 0.45500441896353555 40-41 0.433768208190423 42-43 0.4647352972153661 44-45 0.4720048356740865 46-47 0.47835852676792884 48-49 0.4888907714640278 50-51 0.4784730076885386 52-53 0.4819074353068317 54-55 0.4685704080557934 56-57 0.4463611094574978 58-59 0.42878828814389797 60-61 0.38751791626407545 62-63 0.45752299921695044 64-65 0.4874025194961008 66-67 0.496274790843358 68-69 0.5081808065867743 70-71 0.5001671421440903 72-73 0.4923251990823209 74-75 0.4987361306364681 76-77 0.47578270605420897 78-79 0.45500441896353555 80-81 0.4571795564551212 82-83 0.46925729357945206 84-85 0.46982969818250087 86-87 0.4742372136259771 88-89 0.48511290108390537 90-91 0.500796787207444 92-93 0.4930693250662844 94-95 0.49707615728762644 96-97 0.4979347641921998 98-99 0.49169555401896725 100-101 0.4789881718312826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 873508.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.522377059482842 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89552201544697 18.85989375885553 2 11.637793952862282 5.94048330811041 3 4.387858473074837 3.359657353003879 4 2.2574378334729746 2.304607182969572 5 1.3664997246644532 1.743816061228283 6 0.9320567106467533 1.427298168596864 7 0.6283268365724255 1.122547610771545 8 0.4740462595867012 0.9679029904647416 9 0.3676387063106793 0.8444712315709467 >10 3.016326207034633 16.28082459750842 >50 0.5606302901051152 10.070748512332223 >100 0.4350491051042062 21.53195125325351 >500 0.026461749691905324 4.448606463241901 >1k 0.012109614265787183 5.688534034706693 >5k 0.0013455126961985759 2.6513296169006226 >10k+ 8.970084641323839E-4 2.7573278564848542 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13639 1.561405276196669 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10449 1.196211139451499 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8665 0.9919771770836673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7920 0.9066888912293877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6577 0.7529410148504649 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4933 0.5647343813680012 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3763 0.430791704254569 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3061 0.3504260979865096 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2653 0.30371788237772296 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2253 0.2579255141338145 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2248 0.25735310953076557 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2244 0.25689518584832655 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2117 0.24235610893088558 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2107 0.24121129972478786 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2037 0.23319763528210388 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1958 0.22415364255393197 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1801 0.2061801380181979 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1761 0.20160090119380703 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1753 0.20068505382892887 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1604 0.18362739665807298 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1375 0.15741126583843537 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1197 0.1370336619698961 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1170 0.13394267711343227 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1122 0.12844759292416327 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1118 0.12798966924172417 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1108 0.12684486003562645 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1086 0.1243262797822115 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1081 0.12375387517916264 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1047 0.11986152387843042 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1043 0.11940360019599135 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1032 0.11814431006928386 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1023 0.11711398178379591 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 983 0.11253474495940506 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 959 0.10978720286477055 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 938 0.10738310353196535 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 905 0.10360523315184292 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 901 0.10314730946940384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1448092060977117E-4 0.0 10-11 0.0 0.0 0.0 2.862023015244279E-4 0.0 12-13 0.0 0.0 0.0 4.579236824390847E-4 0.0 14-15 0.0 0.0 0.0 4.579236824390847E-4 0.0 16-17 0.0 0.0 0.0 6.296450633537414E-4 0.0 18-19 0.0 0.0 0.0 8.013664442683982E-4 0.0 20-21 0.0 0.0 0.0 0.0014310115076221397 0.0 22-23 0.0 0.0 0.0 0.0026330611740247372 0.0 24-25 0.0 0.0 0.0 0.004521996364085961 0.0 26-27 0.0 0.0 0.0 0.007841943061769326 0.0 28-29 0.0 0.0 0.0 0.017000416710551018 0.0 30-31 0.0 0.0 0.0 0.03869455116610266 0.0 32-33 0.0 0.0 0.0 0.07567188852305876 0.0 34-35 0.0 0.0 0.0 0.1267876195753216 0.0 36-37 0.0 0.0 0.0 0.19684994298850153 0.0 38-39 0.0 0.0 0.0 0.298680721870893 0.0 40-41 0.0 0.0 0.0 0.41476437536920097 0.0 42-43 0.0 0.0 0.0 0.528844612756838 0.0 44-45 0.0 0.0 0.0 0.6701713092496004 0.0 46-47 0.0 0.0 0.0 0.8387444648474884 0.0 48-49 0.0 0.0 0.0 1.0148733612056215 0.0 50-51 0.0 0.0 0.0 1.207315788750647 0.0 52-53 0.0 0.0 0.0 1.3910576663293295 0.0 54-55 0.0 0.0 0.0 1.5963791974429542 0.0 56-57 0.0 0.0 0.0 1.8528737000691464 0.0 58-59 0.0 0.0 0.0 2.124880367437963 0.0 60-61 0.0 0.0 0.0 2.410624745279952 0.0 62-63 0.0 0.0 0.0 2.69167540537694 0.0 64-65 0.0 0.0 0.0 2.9871506614707592 0.0 66-67 0.0 0.0 0.0 3.3264721101581207 0.0 68-69 0.0 0.0 0.0 3.7174244540404895 0.0 70-71 0.0 0.0 0.0 4.154798811230121 0.0 72-73 0.0 0.0 0.0 4.567731491869565 0.0 74-75 0.0 0.0 0.0 5.018500116770539 0.0 76-77 0.0 0.0 0.0 5.4923366471743815 0.0 78-79 0.0 0.0 0.0 6.086607106059704 0.0 80-81 0.0 0.0 0.0 6.702228256638748 0.0 82-83 0.0 0.0 0.0 7.2935794520485215 0.0 84-85 0.0 0.0 0.0 7.887449227711709 0.0 86-87 0.0 0.0 0.0 8.516350165081487 0.0 88-89 0.0 0.0 0.0 9.194592379234077 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 40 5.4597284E-4 47.371788 4 ATGGGCG 55 5.0421015E-5 43.065266 5 CATATAG 290 0.0 40.83775 3 CATGGGG 1490 0.0 34.972458 4 GACATAT 340 0.0 34.896214 1 CATTCCG 275 0.0 34.436417 9 GACCGTC 30 0.0040052575 31.579382 20-21 GACTGTG 125 2.2122731E-7 30.305784 7 GTACATG 6830 0.0 29.46201 1 GGATGCC 145 2.4721885E-8 29.391384 8 GGCATTC 325 0.0 29.140179 7 ACATGGG 6725 0.0 28.387854 3 TACATGG 7035 0.0 28.281666 2 ATGGGGA 745 0.0 27.977968 5 TGGGGAG 330 0.0 27.263725 6 CATGGGT 540 0.0 27.194914 4 CCGGCGA 35 0.008342457 27.176311 94-95 CGACTCT 35 0.00835183 27.170055 82-83 CCTTTAG 385 0.0 27.119343 1 TTAGGCA 385 0.0 27.069593 4 >>END_MODULE