##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 858467 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.250322959414866 33.0 31.0 34.0 31.0 34.0 2 32.326437708147196 34.0 31.0 34.0 31.0 34.0 3 32.352329210091945 34.0 31.0 34.0 30.0 34.0 4 35.814550821406065 37.0 35.0 37.0 35.0 37.0 5 35.883282642198246 37.0 35.0 37.0 35.0 37.0 6 36.00344451213617 37.0 37.0 37.0 35.0 37.0 7 35.96855441152659 37.0 37.0 37.0 35.0 37.0 8 35.96850898170809 37.0 37.0 37.0 35.0 37.0 9 37.64157154555737 39.0 38.0 39.0 35.0 39.0 10-11 37.57244949427293 39.0 38.0 39.0 35.0 39.0 12-13 37.50911217321108 39.0 38.0 39.0 35.0 39.0 14-15 38.74670604694182 40.0 38.0 41.0 35.0 41.0 16-17 38.70595433487834 40.0 38.0 41.0 35.0 41.0 18-19 38.73465374906665 40.0 38.5 41.0 35.0 41.0 20-21 38.76043575350013 40.5 38.5 41.0 35.0 41.0 22-23 38.75888531533536 41.0 39.0 41.0 35.0 41.0 24-25 38.68940215523718 41.0 38.0 41.0 35.0 41.0 26-27 38.552662478581006 40.0 38.0 41.0 35.0 41.0 28-29 38.42052460956566 40.0 38.0 41.0 35.0 41.0 30-31 38.12659834332595 40.0 37.5 41.0 34.0 41.0 32-33 37.800960316471105 40.0 36.5 41.0 34.0 41.0 34-35 37.45970899289081 40.0 36.0 41.0 33.0 41.0 36-37 37.15379274916799 40.0 35.0 41.0 33.0 41.0 38-39 36.955937153088 40.0 35.0 41.0 33.0 41.0 40-41 36.79216207495454 40.0 35.0 41.0 32.0 41.0 42-43 36.51162129703297 40.0 35.0 41.0 31.0 41.0 44-45 36.10447052711403 40.0 35.0 41.0 30.0 41.0 46-47 35.6631990513322 40.0 35.0 41.0 27.5 41.0 48-49 35.382180095449215 40.0 35.0 41.0 24.5 41.0 50-51 34.26685125927962 38.5 34.0 40.0 23.0 40.5 52-53 34.46640231948345 38.5 35.0 40.0 23.0 40.5 54-55 35.02150111769002 39.0 35.0 41.0 23.0 41.0 56-57 34.95346879961606 39.5 35.0 41.0 22.5 41.0 58-59 34.809440549258156 39.0 35.0 41.0 22.0 41.0 60-61 34.53262559888732 39.0 35.0 41.0 20.0 41.0 62-63 34.22997447776093 39.0 35.0 41.0 17.0 41.0 64-65 33.90814731375813 38.0 35.0 40.0 12.0 41.0 66-67 33.52775004746833 37.5 34.5 40.0 7.5 41.0 68-69 33.095418926994284 37.0 34.0 39.5 2.0 41.0 70-71 32.663669657657195 36.5 34.0 39.0 2.0 41.0 72-73 32.2002692007963 36.0 33.0 39.0 2.0 40.5 74-75 31.732972845782072 35.5 33.0 38.0 2.0 40.0 76-77 31.289996004505706 35.0 32.5 37.0 2.0 39.5 78-79 30.827830306814356 35.0 32.0 37.0 2.0 39.0 80-81 30.31343080164992 35.0 31.0 36.0 2.0 39.0 82-83 29.8809843593289 35.0 31.0 36.0 2.0 37.5 84-85 29.448893783919473 35.0 31.0 35.5 2.0 37.0 86-87 29.064976289129344 35.0 30.5 35.0 2.0 37.0 88-89 28.613391662113976 34.0 29.5 35.0 2.0 36.0 90-91 28.16836116006789 34.0 29.0 35.0 2.0 36.0 92-93 27.7517673946698 34.0 29.0 35.0 2.0 36.0 94-95 27.27704967109976 34.0 27.0 35.0 2.0 35.0 96-97 26.711473475392765 34.0 25.5 35.0 2.0 35.0 98-99 26.12538571663209 34.0 24.5 35.0 2.0 35.0 100-101 23.466289327370767 30.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22863728311912013 1101 2 0.30653701174974657 1101 3 0.37804853573712904 1101 4 0.38358790595334824 1101 5 0.3024438351884555 1101 6 0.38674455611896263 1101 7 0.3552714329879407 1101 8 0.35605441753310174 1101 9 0.47936600298874765 1101 10-11 0.35024137648537135 1101 12-13 0.5122378124522484 1101 14-15 0.5905752020713706 1101 16-17 0.5623941694289698 1101 18-19 0.5548304004776483 1101 20-21 0.5397273789373145 1101 22-23 0.6997670018455509 1101 24-25 0.5470824144772948 1101 26-27 0.4632165101080119 1101 28-29 0.43215091158316454 1101 30-31 0.5301464581755653 1101 32-33 0.4773969247436085 1101 34-35 0.5954505284724263 1101 36-37 0.654414611723908 1101 38-39 0.6691396546986752 1101 40-41 0.7236862536133444 1101 42-43 0.6117320892837768 1101 44-45 0.4975649029097937 1101 46-47 0.4435766541105224 1101 48-49 0.37340683943224207 1101 50-51 0.4660788231819666 1101 52-53 0.3244427550086826 1101 54-55 0.3552811675574432 1101 56-57 0.32331909622404 1101 58-59 0.20829666847062356 1101 60-61 -0.03645516933717374 1101 62-63 -0.0047353123128388575 1101 64-65 0.0724566946959655 1101 66-67 0.27210584192912535 1101 68-69 0.4070628230254485 1101 70-71 0.21051933415425594 1101 72-73 0.2171200095429242 1101 74-75 -0.07170260768595682 1101 76-77 0.0798010001863041 1101 78-79 0.015985381294466805 1101 80-81 -0.06020518560503163 1101 82-83 -0.04660299635726517 1101 84-85 0.03064662457028433 1101 86-87 -0.16496406647150508 1101 88-89 0.0919742169587856 1101 90-91 0.1827018756462202 1101 92-93 -0.012192422537495418 1101 94-95 -0.32912382742293644 1101 96-97 -0.41712503018927904 1101 98-99 -0.7857786851915378 1101 100-101 -1.1042460086269728 1102 1 0.13489347501244708 1102 2 0.08349870068456511 1102 3 0.27861498470815604 1102 4 0.11768518132602424 1102 5 0.21952394226207161 1102 6 0.14893505858017875 1102 7 0.2485474739059086 1102 8 0.3447234955577585 1102 9 0.18407630979584866 1102 10-11 0.2628419522585972 1102 12-13 0.224712510676369 1102 14-15 0.2775606678765783 1102 16-17 0.2614739812342606 1102 18-19 0.3575260837324308 1102 20-21 0.4433737484967111 1102 22-23 0.5265756174644665 1102 24-25 0.6322197336740061 1102 26-27 0.5151050618557349 1102 28-29 0.4852263642955563 1102 30-31 0.4770923735656467 1102 32-33 0.6097324971950684 1102 34-35 0.47137955529134246 1102 36-37 0.6931744070563468 1102 38-39 0.5608587379682319 1102 40-41 0.6841882874681815 1102 42-43 0.7107858708551476 1102 44-45 0.7233480438654567 1102 46-47 0.8749248259722364 1102 48-49 0.4546512788692141 1102 50-51 0.6444072936531953 1102 52-53 0.8789955206239028 1102 54-55 0.9468386302701006 1102 56-57 0.9429950392372604 1102 58-59 0.8409721880069014 1102 60-61 0.8901463699432242 1102 62-63 0.7723769246166867 1102 64-65 0.6642190969368968 1102 66-67 0.6514246268222692 1102 68-69 0.6122150610428037 1102 70-71 0.5323520957289674 1102 72-73 0.603560200224095 1102 74-75 0.6391879371229514 1102 76-77 0.4917488775567094 1102 78-79 0.27933850278573047 1102 80-81 0.30467361840284823 1102 82-83 -0.08854663895826675 1102 84-85 -0.23176824296691123 1102 86-87 -0.2393755695038351 1102 88-89 -0.24310385431743597 1102 90-91 -0.35626188793709446 1102 92-93 -0.8120830567039228 1102 94-95 -0.9168156061426345 1102 96-97 -0.913768295546145 1102 98-99 -0.7374586065569204 1102 100-101 -0.8208097846426377 1103 1 0.04208083808642016 1103 2 0.12808738418957688 1103 3 0.25887907091492224 1103 4 0.14864624420616934 1103 5 0.1310632321405052 1103 6 0.16883435529098278 1103 7 0.03864869704984386 1103 8 0.17420000183685858 1103 9 0.1343299246428984 1103 10-11 0.2141259934607973 1103 12-13 0.20168108917071947 1103 14-15 0.30194185507627225 1103 16-17 0.274008050285822 1103 18-19 0.28428040780988084 1103 20-21 0.29676914258372733 1103 22-23 0.2411048147344914 1103 24-25 0.3268682004738608 1103 26-27 0.2876574743530398 1103 28-29 0.21567425628458636 1103 30-31 0.4596122580093649 1103 32-33 0.29330805808120175 1103 34-35 0.3763389694893178 1103 36-37 0.263141284883055 1103 38-39 0.22657171874103454 1103 40-41 0.13468140681940355 1103 42-43 0.0548443025276697 1103 44-45 0.3338784442278637 1103 46-47 0.6079051531836583 1103 48-49 0.5676621289802384 1103 50-51 0.5347940655780619 1103 52-53 0.3464526140418016 1103 54-55 0.15695648876564405 1103 56-57 0.2603013747812071 1103 58-59 0.32724616630667924 1103 60-61 0.2685356371708423 1103 62-63 0.2136853247265904 1103 64-65 0.35184766815327606 1103 66-67 0.2953631205802978 1103 68-69 0.25148600190560444 1103 70-71 0.2688284460516499 1103 72-73 0.3889734476081159 1103 74-75 0.4543888609790905 1103 76-77 0.3602701878877106 1103 78-79 0.4134610251209203 1103 80-81 0.4551511421119372 1103 82-83 0.5409231834742059 1103 84-85 0.5802002983949599 1103 86-87 0.6951895720080508 1103 88-89 0.5474299622332559 1103 90-91 0.5946991006780884 1103 92-93 0.5788569891918236 1103 94-95 0.438772460963186 1103 96-97 0.3065832747864867 1103 98-99 0.22014538295686492 1103 100-101 0.014409259864354595 1104 1 0.25889200442956906 1104 2 0.26425117319303837 1104 3 0.23978468050862034 1104 4 0.25406172334501775 1104 5 0.1789700712438389 1104 6 0.2504434980514958 1104 7 0.32860687963026436 1104 8 0.2746287448834366 1104 9 0.33955586905779 1104 10-11 0.3720818720317567 1104 12-13 0.36221929028186395 1104 14-15 0.4031271516910735 1104 16-17 0.36956455312355985 1104 18-19 0.38892612231703794 1104 20-21 0.4196760697608255 1104 22-23 0.32232202927863796 1104 24-25 0.43013379921686834 1104 26-27 0.48179581356382783 1104 28-29 0.5338274905290774 1104 30-31 0.3571807177402775 1104 32-33 0.14920618140559583 1104 34-35 0.16147423316452603 1104 36-37 0.3459403040937019 1104 38-39 0.25592846325315577 1104 40-41 0.15632729395112932 1104 42-43 0.07045713734005687 1104 44-45 -0.2491513282247766 1104 46-47 -0.2045030299252062 1104 48-49 -0.11540798125287921 1104 50-51 -0.3716125956162841 1104 52-53 -0.300965239565528 1104 54-55 -0.3945340714891401 1104 56-57 -0.3886841916915813 1104 58-59 -0.44296588006943693 1104 60-61 -0.35112841429241826 1104 62-63 -0.33491465484029703 1104 64-65 -0.27531410219967967 1104 66-67 -0.4368776871617399 1104 68-69 -0.3273135880229816 1104 70-71 -0.4376137969403189 1104 72-73 -0.12468591128539686 1104 74-75 -0.1723563205056955 1104 76-77 -0.14344828611115545 1104 78-79 -0.06449851731830236 1104 80-81 -0.16463797408553305 1104 82-83 -0.12655611038564274 1104 84-85 0.008125139774126211 1104 86-87 -0.11397925833723122 1104 88-89 -0.021175971119571813 1104 90-91 -0.06044722506094047 1104 92-93 -0.3585947904265012 1104 94-95 -0.38848231527662946 1104 96-97 -0.5085499103480728 1104 98-99 -0.3987031055213208 1104 100-101 -0.6012870688691798 1105 1 0.23899121431291093 1105 2 0.2895829650539312 1105 3 0.3651783105081847 1105 4 0.31556546556063836 1105 5 0.3762001259235461 1105 6 0.3671092125004449 1105 7 0.24987583856503903 1105 8 0.2916207899446164 1105 9 0.35635635031982815 1105 10-11 0.3215308740515752 1105 12-13 0.19567977268656023 1105 14-15 0.4284186143310862 1105 16-17 0.36010062929201325 1105 18-19 0.3756166392368172 1105 20-21 0.34916966917739956 1105 22-23 0.25432108154194566 1105 24-25 0.29912432521073384 1105 26-27 0.3825094727348599 1105 28-29 0.2924965926325811 1105 30-31 0.5821429931149211 1105 32-33 0.5104593783365416 1105 34-35 0.4864042451624684 1105 36-37 0.4716120376788666 1105 38-39 0.5286396959494013 1105 40-41 0.43088862965492325 1105 42-43 0.20524747580304137 1105 44-45 0.1464800089241507 1105 46-47 0.1703318723268481 1105 48-49 0.3018574623181465 1105 50-51 0.3308795821952444 1105 52-53 0.22721480351952295 1105 54-55 0.22544452041611862 1105 56-57 0.3356118113465527 1105 58-59 0.19282764776276906 1105 60-61 0.46808241007046547 1105 62-63 0.40350245684812336 1105 64-65 0.4777450372419523 1105 66-67 0.43236489646258036 1105 68-69 0.24903130747392765 1105 70-71 0.26992037367244137 1105 72-73 0.2187081623356022 1105 74-75 0.11949448826322495 1105 76-77 0.2184653018115874 1105 78-79 -0.08045880647881631 1105 80-81 -0.06694967863365875 1105 82-83 0.11025287362046043 1105 84-85 0.14268358521709246 1105 86-87 0.1192740326952304 1105 88-89 0.2781180994767247 1105 90-91 0.229150188491694 1105 92-93 0.25230720620600877 1105 94-95 0.29887716259439046 1105 96-97 0.373898812727969 1105 98-99 0.4366864579484684 1105 100-101 0.09214867828005069 1106 1 0.18545803763460356 1106 2 0.20846530501264482 1106 3 0.21105378274519637 1106 4 0.23445754236487915 1106 5 0.07837274072095113 1106 6 0.2093074027454236 1106 7 0.22271328517466316 1106 8 0.2411969126029092 1106 9 0.4083089124084438 1106 10-11 0.25990313312955493 1106 12-13 0.3758876867945986 1106 14-15 0.14623833988186874 1106 16-17 0.19496210643639245 1106 18-19 0.2894569160228855 1106 20-21 0.24459919388950624 1106 22-23 0.3242997903064051 1106 24-25 0.28998791849620176 1106 26-27 0.2614772372849501 1106 28-29 0.234609423198485 1106 30-31 0.2515397630787035 1106 32-33 0.1181148108472243 1106 34-35 0.09609305557959402 1106 36-37 0.2251059424135704 1106 38-39 0.19389830656645302 1106 40-41 0.5259543209060453 1106 42-43 0.6512501761548606 1106 44-45 0.3640563133368886 1106 46-47 0.30856586819842136 1106 48-49 0.579609094862569 1106 50-51 0.6916832614246076 1106 52-53 0.43957702715538716 1106 54-55 0.3339665321782306 1106 56-57 0.47355207904489305 1106 58-59 0.5626930845576581 1106 60-61 0.5480024381125759 1106 62-63 0.491405075410448 1106 64-65 0.23502495207701912 1106 66-67 0.3565830277671864 1106 68-69 0.22939695847941977 1106 70-71 -0.04029227903374277 1106 72-73 0.0019108055645915556 1106 74-75 -0.07019062215260519 1106 76-77 -0.10435332023003596 1106 78-79 -0.05168642796219203 1106 80-81 -0.07625323452964494 1106 82-83 -0.049387577130573845 1106 84-85 0.0014214303882660317 1106 86-87 -0.08105020426064513 1106 88-89 0.025494019608625962 1106 90-91 0.11299124422539819 1106 92-93 0.06563078105638454 1106 94-95 0.22022617008527234 1106 96-97 0.2290632355926796 1106 98-99 0.34775660218360827 1106 100-101 0.22529030394377614 1107 1 -0.020469918462232783 1107 2 0.1570428271604527 1107 3 0.02272987469478238 1107 4 0.09495048787837845 1107 5 0.07585879580577881 1107 6 -0.03306712518622135 1107 7 -0.05131314421934974 1107 8 0.11288439209567969 1107 9 0.18389549955531947 1107 10-11 0.12445892244070933 1107 12-13 0.12502638321041815 1107 14-15 0.05315321638813941 1107 16-17 0.028371839247313346 1107 18-19 -0.03381661662462676 1107 20-21 0.042386665018547376 1107 22-23 0.1866198896418112 1107 24-25 0.11631984286233177 1107 26-27 0.2299717011375435 1107 28-29 0.2787015722273267 1107 30-31 0.5786348780156843 1107 32-33 0.4457841281736563 1107 34-35 0.5566500158132683 1107 36-37 0.47230328789361664 1107 38-39 0.5123884277119046 1107 40-41 0.44184814654152405 1107 42-43 0.5388239217558635 1107 44-45 0.6074328807324889 1107 46-47 0.837737601356757 1107 48-49 0.5524020345858034 1107 50-51 0.39767153289849233 1107 52-53 0.20078684103018674 1107 54-55 0.20804008798683782 1107 56-57 0.11494821114040121 1107 58-59 0.05334259270586017 1107 60-61 0.09605851372072038 1107 62-63 0.24399059457304162 1107 64-65 0.1271231551612999 1107 66-67 -0.015884530602015445 1107 68-69 0.1092334475132759 1107 70-71 0.1167448366366699 1107 72-73 0.28369459665142926 1107 74-75 0.23825176138242554 1107 76-77 0.1254852597368945 1107 78-79 0.23190039079572955 1107 80-81 0.1896370526037181 1107 82-83 0.23286024025698282 1107 84-85 0.41027926624036937 1107 86-87 0.20292665393549214 1107 88-89 0.32322278377067803 1107 90-91 0.47519312542180003 1107 92-93 0.4607552076581989 1107 94-95 0.5600011856868505 1107 96-97 0.4966463743083942 1107 98-99 0.35609138308598887 1107 100-101 0.2922773658932982 1108 1 0.007233061570154575 1108 2 0.16875186919302365 1108 3 0.1750917544560906 1108 4 0.23812464860257165 1108 5 0.20044552886578515 1108 6 0.2047491114121982 1108 7 0.18312027977336243 1108 8 0.1295458717830158 1108 9 0.32833257698564466 1108 10-11 0.10260595300781006 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-0.9938966897615842 2316 96-97 -1.173710076850437 2316 98-99 -1.3218639777011063 2316 100-101 -1.2983674164563368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3057.0 3 567.0 4 474.0 5 507.0 6 529.0 7 729.0 8 873.0 9 1071.0 10 1399.0 11 2102.0 12 3565.0 13 6353.0 14 5644.0 15 6363.0 16 9204.0 17 14854.0 18 11156.0 19 5686.0 20 4968.0 21 4796.0 22 5054.0 23 5722.0 24 6178.0 25 7267.0 26 7866.0 27 9238.0 28 10595.0 29 12314.0 30 14723.0 31 17763.0 32 21833.0 33 28054.0 34 39428.0 35 69492.0 36 91078.0 37 160221.0 38 220704.0 39 47030.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.402991724346 23.262574562157447 15.550558434162538 18.78387527933402 2 14.171458826300881 24.30059348530631 40.885994609767955 20.64195307862485 3 18.03120579286248 30.889629829183328 32.486422249667335 18.592742128286854 4 10.264712571951781 20.157579504166932 42.25498486606909 27.322723057812198 5 9.536301328319281 41.95111766380039 36.02220397422678 12.490377033653555 6 23.62204265477978 42.45008532821053 19.43396622308431 14.493905793925377 7 23.246471024393884 37.825558783616785 23.786251568044992 15.141718623944339 8 24.093445762962805 34.93490762793767 21.588610612732154 19.38303599636737 9 25.724621208146953 18.657764752102196 24.628086263236 30.989527776514848 10-11 22.152814369582146 29.6053214457247 28.872177120394678 19.36968706429847 12-13 24.497914949526272 29.338531289746822 28.8381658084365 17.32538795229041 14-15 21.861759886971075 29.501062851940386 29.923289309638758 18.713887951449788 16-17 16.99749771075215 32.846611942325524 30.814645823776587 19.34124452314574 18-19 16.71980138167747 31.29206255381238 34.91711509101688 17.07102097349327 20-21 18.301790114353032 29.459153343355815 34.76321746079953 17.47583908149162 22-23 17.974946554320457 29.59634301544984 34.73552331875662 17.693187111473083 24-25 18.121602112142252 30.597783370145 34.68737682474304 16.59323769296971 26-27 17.465267189148122 29.739169374602408 36.22288006969011 16.57268336655936 28-29 16.418912064218738 30.794781527783773 35.85682318274098 16.929483225256508 30-31 17.12720162488253 30.139903598387242 35.31921666110892 17.413678115621305 32-33 17.19305732146178 30.65797811655972 35.19643969986493 16.95252486211357 34-35 17.986554981336752 30.250473813304406 35.043892381409336 16.719078823949502 36-37 17.866427920637634 29.514958320939304 35.36085939201739 17.25775436640567 38-39 16.863947414177165 31.554479401876073 34.65024257365171 16.93133061029505 40-41 16.81423439389346 31.212096027493953 35.09874200658553 16.874927572027058 42-43 16.6000738814022 31.239363449078912 33.947567621680385 18.2129950478385 44-45 17.29343778542823 30.37723365886789 34.011686456357296 18.31764209934659 46-47 18.357424849282875 31.45326027326682 33.14541384928932 17.04390102816099 48-49 17.49949206657763 31.41985396109025 33.12124063397117 17.959413338360946 50-51 16.463981582102978 31.478467173798997 33.741006520272805 18.316544723825228 52-53 17.24133489310527 32.673826160373125 32.10278803378569 17.98205091273591 54-55 17.26143673372845 31.457523797925912 32.35370401053379 18.92733545781185 56-57 17.89522910950245 31.48657791970632 31.905125347667703 18.713067623123532 58-59 18.52851961811926 30.966915803291506 32.300794580210344 18.203769998378885 60-61 18.017913150699997 31.93646166138857 32.65423945736443 17.391385730547 62-63 17.35621418159893 31.64183647784015 33.03230753920633 17.969641801354584 64-65 17.178198255696504 32.154733011485234 32.4294066499009 18.23766208291736 66-67 17.682935041861157 31.446726502618482 31.262030288071045 19.608308167449312 68-69 17.3789030287128 31.210348882974735 32.08103352949996 19.329714558812505 70-71 18.32601167443928 31.308686253690354 32.12983260233875 18.235469469531612 72-73 18.29147035159283 30.34583074215003 32.976482010453736 18.386216895803393 74-75 17.183067744481896 32.52073607503168 31.716356728420074 18.579839452066345 76-77 17.39578954517299 31.250080501910382 32.22974484114485 19.12438511177178 78-79 17.544166674470656 31.126033638313217 32.24413062004252 19.085669067173612 80-81 18.079621536635248 30.9409722181573 31.74811020017771 19.23129604502974 82-83 17.926809778132153 30.770428626039774 32.50069225766153 18.80206933816655 84-85 17.47399071322698 30.874656663365013 32.08455974467228 19.566792878735725 86-87 17.25896140069065 31.021406205089086 32.28404858091033 19.435583813309933 88-89 17.874917961859364 30.460695753688277 31.740637113615726 19.923749170836633 90-91 18.067306853362737 29.773424503039415 32.290214325324065 19.86905431827378 92-93 18.883461686888378 30.33631539897157 32.19746634708574 18.582756567054314 94-95 18.502102811126452 29.222516182452097 32.552581989724274 19.722799016697177 96-97 18.365798128004048 29.237229577161273 32.7025419044139 19.694430390420777 98-99 18.47371943781925 29.70907772820513 31.641727767116954 20.17547506685866 100-101 18.511638417814485 28.4662486176104 32.65852620436944 20.363586760205678 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5969.0 1 3698.0 2 1954.5 3 2513.0 4 2103.0 5 1921.0 6 3236.0 7 6104.0 8 8729.5 9 8690.5 10 6099.5 11 3943.0 12 3498.5 13 3419.5 14 3220.0 15 2986.0 16 2814.0 17 2665.0 18 2545.0 19 2581.0 20 2849.5 21 3148.0 22 3544.0 23 4103.5 24 4631.0 25 5812.5 26 8417.0 27 11043.0 28 11337.0 29 12127.0 30 15135.0 31 18347.0 32 23163.5 33 29027.5 34 35405.0 35 39523.5 36 43739.0 37 48161.0 38 45873.5 39 42258.0 40 38739.0 41 34573.0 42 31106.0 43 28826.5 44 27756.5 45 26700.0 46 24790.0 47 25601.0 48 34121.5 49 31431.5 50 18327.0 51 10834.0 52 6843.0 53 5320.0 54 4481.0 55 3840.0 56 3235.5 57 2760.5 58 2507.5 59 2397.5 60 2213.5 61 1991.5 62 1593.0 63 962.5 64 558.0 65 448.5 66 407.0 67 397.5 68 345.0 69 272.0 70 231.5 71 219.0 72 208.0 73 222.5 74 223.0 75 163.0 76 110.0 77 78.5 78 74.0 79 61.0 80 35.5 81 22.5 82 18.0 83 19.5 84 16.5 85 7.5 86 7.5 87 7.0 88 3.5 89 2.5 90 1.5 91 1.0 92 0.5 93 0.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38533805026867657 2 0.1153218469667442 3 0.1162537406796068 4 0.13069809322897677 5 0.13314431422524103 6 0.07024148860701693 7 0.08282205373066175 8 0.07990988587796619 9 0.07851204530867233 10-11 0.07629879774062369 12-13 0.08084177959082878 14-15 0.07379433338730551 16-17 0.07600758095535415 18-19 0.08637489851095033 20-21 0.08486057122754864 22-23 0.0955773489254683 24-25 0.11054589168832349 26-27 0.11141954204413217 28-29 0.10460506926882455 30-31 0.09377180485679705 32-33 0.09161680064580234 34-35 0.33880160798260156 36-37 0.5312959030457781 38-39 0.5121338385750412 40-41 0.4857495978296196 42-43 0.5120173518609334 44-45 0.5226758862017993 46-47 0.5202879085625889 48-49 0.52611224426798 50-51 0.5259375141968183 52-53 0.5237242666287697 54-55 0.5187735812791872 56-57 0.4895936593951777 58-59 0.479517558624851 60-61 0.4282051610603552 62-63 0.5046204455150868 64-65 0.529373872262999 66-67 0.5396247031044874 68-69 0.5557581130084208 70-71 0.5499920206600837 72-73 0.5370037520370614 74-75 0.540614840174404 76-77 0.5183658777798098 78-79 0.4895936593951777 80-81 0.4980971895250487 82-83 0.5099205910069927 84-85 0.5066007196549198 86-87 0.5061930161555424 88-89 0.5176087141381089 90-91 0.536013614967145 92-93 0.54055659681735 94-95 0.5370037520370614 96-97 0.5380521324640318 98-99 0.5374114555364389 100-101 0.5149295197136291 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 858467.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.86912066692923 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2049368938726 19.19616466589459 2 11.51677797062834 5.958578380328338 3 4.306383941699028 3.342070974778155 4 2.228111685227232 2.305571601781532 5 1.3559148868222004 1.7538162910644597 6 0.8887219240890862 1.3794272816163653 7 0.6353590146227865 1.1505325311268684 8 0.48550423633308587 1.004765413920474 9 0.3751475678495439 0.8734263930544384 >10 2.976233814376976 16.203780770176177 >50 0.5618506201165946 10.23069159339773 >100 0.4267903748845713 21.45411373609703 >500 0.02386064332160578 4.128962479973552 >1k 0.012155422069497284 5.676639189881558 >5k 0.001350602452166365 2.6264734112307435 >10k+ 9.004016347775766E-4 2.714985285678037 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13213 1.5391389535066577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10099 1.1763993257748988 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8220 0.9575207899663004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7656 0.8918222832094886 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6676 0.7776653033838226 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4762 0.5547097325814504 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3735 0.43507787719271673 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3018 0.35155690317740806 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2739 0.31905710994132563 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2227 0.25941591231812056 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2193 0.2554553640384546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2155 0.2510288689023573 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2035 0.23705046320941864 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2004 0.23343937507207618 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1926 0.224353411371666 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1915 0.22307205751647996 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1788 0.20827824482478652 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1725 0.2009395818359937 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1707 0.1988428209820529 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.1827676544351734 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1337 0.15574273676215858 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.13955108350117126 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1137 0.1324453939405941 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1119 0.1303486330866533 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1106 0.12883430580325161 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1078 0.12557267780823259 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1059 0.12335943024018396 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1051 0.12242753652732137 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1050 0.12231104981321354 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1048 0.1220780763849979 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1035 0.12056374910159622 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1026 0.11951536867462582 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 985 0.11473941339620511 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 959 0.11171075882940172 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 916 0.10670183012276536 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 890 0.10367317555596196 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 884 0.10297425527131504 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 863 0.10052803427505075 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.494601423234673E-4 0.0 18-19 0.0 0.0 0.0 3.494601423234673E-4 0.0 20-21 0.0 0.0 0.0 5.824335705391122E-4 0.0 22-23 0.0 0.0 0.0 0.0014560839263477803 0.0 24-25 0.0 0.0 0.0 0.0039023049226120515 0.0 26-27 0.0 0.0 0.0 0.006872716132361524 0.0 28-29 0.0 0.0 0.0 0.01590043647571776 0.0 30-31 0.0 0.0 0.0 0.037566965299772735 0.0 32-33 0.0 0.0 0.0 0.07379433338730551 0.0 34-35 0.0 0.0 0.0 0.12487375752358565 0.0 36-37 0.0 0.0 0.0 0.198784577624999 0.0 38-39 0.0 0.0 0.0 0.3080491154581364 0.0 40-41 0.0 0.0 0.0 0.42925354148732564 0.0 42-43 0.0 0.0 0.0 0.5469051227362263 0.0 44-45 0.0 0.0 0.0 0.6799911936044134 0.0 46-47 0.0 0.0 0.0 0.8478485486337857 0.0 48-49 0.0 0.0 0.0 1.024617137292406 0.0 50-51 0.0 0.0 0.0 1.221421440777572 0.0 52-53 0.0 0.0 0.0 1.4093727539905436 0.0 54-55 0.0 0.0 0.0 1.6220192505943736 0.0 56-57 0.0 0.0 0.0 1.8818428664118714 0.0 58-59 0.0 0.0 0.0 2.1580328655615184 0.0 60-61 0.0 0.0 0.0 2.4484924871893736 0.0 62-63 0.0 0.0 0.0 2.731147498972005 0.0 64-65 0.0 0.0 0.0 3.034362415794667 0.0 66-67 0.0 0.0 0.0 3.3763091650581796 0.0 68-69 0.0 0.0 0.0 3.767588037746355 0.0 70-71 0.0 0.0 0.0 4.213324449279938 0.0 72-73 0.0 0.0 0.0 4.634598650850878 0.0 74-75 0.0 0.0 0.0 5.085809937947527 0.0 76-77 0.0 0.0 0.0 5.550708413951847 0.0 78-79 0.0 0.0 0.0 6.1284242725695925 0.0 80-81 0.0 0.0 0.0 6.743940069915325 0.0 82-83 0.0 0.0 0.0 7.323228499173527 0.0 84-85 0.0 0.0 0.0 7.902109224932351 0.0 86-87 0.0 0.0 0.0 8.519255836275594 0.0 88-89 0.0 0.0 0.0 9.199538246665277 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 20 5.355746E-4 47.559532 94-95 TAGCACG 25 0.001643196 37.8713 10-11 CATTCCG 310 0.0 33.59453 9 CCTTTAG 315 0.0 33.16374 1 GGATATG 505 0.0 30.089155 1 TAGGCAT 335 0.0 29.689983 5 GTCGGGA 160 1.9645086E-9 29.601622 2 GGGACAT 80 4.5820663E-4 29.587816 7 GGCATTC 370 0.0 28.148407 7 GACATAT 340 0.0 27.932028 1 CATGGGT 495 0.0 27.747782 4 CGACGAG 35 0.008341572 27.176876 94-95 GGTCGGG 175 4.974936E-9 27.13397 1 CATATAG 300 0.0 26.907854 1 CTTTAGG 395 0.0 26.379166 2 CCGCGAT 90 9.1261865E-4 26.298748 9 GTACATG 6540 0.0 25.993015 1 GTATAGG 480 0.0 25.720741 1 TCGGGAG 185 9.242285E-9 25.601402 3 GATTGGC 540 0.0 25.422121 9 >>END_MODULE