FastQCFastQC Report
Fri 27 May 2016
SRR939213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499040
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92480.616928167360444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71900.4796403031273348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.32747625146760595No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33830.22567776710428009No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22950.1530979827089337No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21940.1463603372825275No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20950.13975611057743623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.11447326288824848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16640.11100437613405913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG14400.025.005182
GTATCAA135500.023.2271271
GTACATG84800.022.8394471
GAGTACT66850.022.59849712-13
TACATGG86150.022.437982
GTACCTG17200.021.5271931
AGTACTT67550.021.45291712-13
GGTATCA101700.021.2379071
ACATGGG87100.021.2141363
GTACTTT72450.020.81976714-15
ATAACGC1404.937398E-420.3049583
TCAACGC156450.020.0475524
ATCAACG156350.019.9997673
TATCAAC157100.019.9344462
GTACACG2151.0857475E-619.8712561
CAACGCA157950.019.827175
AACGCAG161400.019.4536156
GTGTAGC8300.019.4456351
ATGGGAT24350.019.0680375
CATGGGA57400.018.2414464
Produced by FastQC (version 0.11.5)