##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499040 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02142371117515 33.0 31.0 34.0 30.0 34.0 2 32.109810945671896 34.0 31.0 34.0 30.0 34.0 3 32.15602252108016 34.0 31.0 34.0 30.0 34.0 4 35.608421389689404 37.0 35.0 37.0 33.0 37.0 5 35.66422577116021 37.0 35.0 37.0 35.0 37.0 6 35.75903244743302 37.0 36.0 37.0 35.0 37.0 7 35.68514449247518 37.0 36.0 37.0 35.0 37.0 8 35.67765970220941 37.0 36.0 37.0 33.0 37.0 9 37.3411830238019 39.0 38.0 39.0 34.0 39.0 10-11 37.24786730173978 39.0 37.0 39.0 34.0 39.0 12-13 37.14758645533141 39.0 37.0 39.0 34.0 39.0 14-15 38.412428954530895 40.0 38.0 41.0 34.0 41.0 16-17 38.35285215871491 40.0 38.0 41.0 34.0 41.0 18-19 38.31241027590991 40.0 38.0 41.0 33.5 41.0 20-21 38.22677713736792 40.0 38.0 41.0 33.5 41.0 22-23 38.14040552620344 40.0 38.0 41.0 33.5 41.0 24-25 38.1779729026577 40.0 38.0 41.0 33.0 41.0 26-27 38.08886387287864 40.0 38.0 41.0 33.0 41.0 28-29 37.9523645132885 40.0 38.0 41.0 33.0 41.0 30-31 37.760636140463234 40.0 37.5 41.0 33.0 41.0 32-33 37.528251080691646 40.0 37.0 41.0 32.5 41.0 34-35 37.20821292293735 40.0 37.0 41.0 31.5 41.0 36-37 36.938186439321164 40.0 36.0 41.0 31.0 41.0 38-39 36.689948567082936 40.0 36.0 41.0 30.0 41.0 40-41 36.5304384806276 40.0 36.0 41.0 30.0 41.0 42-43 36.314422563774144 39.5 35.0 41.0 30.0 41.0 44-45 36.02082199274202 39.0 35.0 41.0 28.5 41.0 46-47 35.60305462162451 39.0 35.0 41.0 26.5 41.0 48-49 35.49892497865301 39.0 35.0 41.0 26.0 41.0 50-51 34.486760860283916 38.0 34.0 40.0 24.0 40.5 52-53 34.643800699114095 38.5 34.5 39.5 24.0 40.5 54-55 35.14190481908422 39.0 35.0 41.0 25.0 41.0 56-57 35.042202009285944 39.0 35.0 41.0 24.5 41.0 58-59 34.86408834987726 39.0 35.0 41.0 24.0 41.0 60-61 34.57478419521827 38.5 35.0 40.5 23.0 41.0 62-63 34.21757191269079 38.0 34.0 40.0 22.5 41.0 64-65 33.85487111751521 37.5 34.0 40.0 21.5 41.0 66-67 33.46287090404525 37.0 34.0 40.0 20.0 41.0 68-69 33.0600754482869 36.0 33.0 39.0 18.5 41.0 70-71 32.643304047924005 36.0 33.0 39.0 17.0 41.0 72-73 32.19130977158715 35.0 32.5 38.5 12.5 40.0 74-75 31.73744529832426 35.0 32.0 37.5 8.0 39.5 76-77 31.291754723022734 35.0 31.5 37.0 7.0 39.0 78-79 30.86565635339951 35.0 31.0 36.5 2.0 39.0 80-81 30.434800605720994 35.0 31.0 36.0 2.0 38.5 82-83 30.01040299124773 35.0 30.5 35.5 2.0 37.0 84-85 29.622196205571566 34.0 30.0 35.0 2.0 37.0 86-87 29.24161129789732 34.0 30.0 35.0 2.0 36.0 88-89 28.84394879389476 34.0 29.0 35.0 2.0 36.0 90-91 28.45669061532714 34.0 29.0 35.0 2.0 36.0 92-93 28.05173711175152 34.0 29.0 35.0 2.0 35.0 94-95 27.598148148148148 34.0 27.5 35.0 2.0 35.0 96-97 27.040160702849825 34.0 26.5 35.0 2.0 35.0 98-99 26.35535609456719 34.0 25.0 35.0 2.0 35.0 100-101 23.812690121677875 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30405027796199846 1101 2 0.39451067483276603 1101 3 0.3258649634904458 1101 4 0.13575031493435574 1101 5 0.2111596013204533 1101 6 0.33494860899816103 1101 7 0.22618117636856283 1101 8 0.27639477109101307 1101 9 0.4234972558987522 1101 10-11 0.39884051334120585 1101 12-13 0.3182018533012396 1101 14-15 0.3921408865450857 1101 16-17 0.4954389754711457 1101 18-19 0.5466900157894017 1101 20-21 0.6155696692359456 1101 22-23 0.5899867185624501 1101 24-25 0.529459974725178 1101 26-27 0.5724934038746099 1101 28-29 0.448157116769778 1101 30-31 0.45699142781152347 1101 32-33 0.45613253446654056 1101 34-35 0.4575973374877478 1101 36-37 0.26927483901432225 1101 38-39 0.4706720485293445 1101 40-41 0.36375972020670844 1101 42-43 0.3945464665947114 1101 44-45 0.33354436088985295 1101 46-47 0.3252241762165795 1101 48-49 0.28226460147691057 1101 50-51 0.27744755681664657 1101 52-53 0.35417357431190766 1101 54-55 0.26883384200100835 1101 56-57 0.3312846303871311 1101 58-59 0.374213178086201 1101 60-61 0.3110573402515868 1101 62-63 0.39684005760177143 1101 64-65 0.3233450871347685 1101 66-67 0.13645726996738006 1101 68-69 0.24948308783984174 1101 70-71 0.35951183229594363 1101 72-73 0.30186370066514456 1101 74-75 0.2072661686439865 1101 76-77 0.17465636141888297 1101 78-79 0.1984567594850084 1101 80-81 0.14828461300965756 1101 82-83 0.15694593305431326 1101 84-85 -0.12635527380130895 1101 86-87 -0.0566853366103679 1101 88-89 0.09434703046204262 1101 90-91 -0.0867850854203347 1101 92-93 -0.0812561935777012 1101 94-95 -0.16026197197549763 1101 96-97 -0.27650762746325697 1101 98-99 -0.4697093640984029 1101 100-101 -0.5761493654867849 1102 1 0.25234912504028983 1102 2 0.4346451631618251 1102 3 0.44477398568471216 1102 4 0.42137958250759766 1102 5 0.27872581423879694 1102 6 0.2893456353220145 1102 7 0.37651440139224235 1102 8 0.48293264144665926 1102 9 0.5472329711227104 1102 10-11 0.3382355002173014 1102 12-13 0.3186618812773858 1102 14-15 0.48090813831433366 1102 16-17 0.4648777931906096 1102 18-19 0.5364953695126005 1102 20-21 0.5911256780021219 1102 22-23 0.5073349294943483 1102 24-25 0.4327198648945867 1102 26-27 0.5235054654221472 1102 28-29 0.6002999195932048 1102 30-31 0.6260524291918941 1102 32-33 0.5239398515902991 1102 34-35 0.5100007748213926 1102 36-37 0.5787742870959747 1102 38-39 0.5122436561903001 1102 40-41 0.5390061822833516 1102 42-43 0.48175619095815847 1102 44-45 0.43101556089169435 1102 46-47 0.5842470042047196 1102 48-49 0.4988139134801557 1102 50-51 0.5264180389789672 1102 52-53 0.4400098400715393 1102 54-55 0.43715482342474843 1102 56-57 0.5456150347508526 1102 58-59 0.43145742327161685 1102 60-61 0.5745787636708926 1102 62-63 0.558454194085769 1102 64-65 0.5787035344812992 1102 66-67 0.5503335227298507 1102 68-69 0.41583894036357094 1102 70-71 0.3215222208049724 1102 72-73 0.22473772040515883 1102 74-75 0.15290797248368193 1102 76-77 0.15071877543368828 1102 78-79 0.07650239241667478 1102 80-81 0.010105978126112802 1102 82-83 -0.06325738951718662 1102 84-85 0.002513627731385526 1102 86-87 -0.28151025520529416 1102 88-89 -0.18089516836221975 1102 90-91 -0.24011468552726356 1102 92-93 -0.40176750732772604 1102 94-95 -0.42969347303209204 1102 96-97 -0.29608603789232646 1102 98-99 -0.32235235012645447 1102 100-101 -0.4779136606329004 1103 1 0.10123673204863337 1103 2 0.1727614571603766 1103 3 0.274101567015542 1103 4 0.20376153103585182 1103 5 0.16801285921484777 1103 6 0.2252079643443352 1103 7 0.2423796932046116 1103 8 0.3092536642377013 1103 9 0.2523298678245922 1103 10-11 0.28365956076595467 1103 12-13 0.32362062667047553 1103 14-15 0.41402764200211095 1103 16-17 0.3610690367992859 1103 18-19 0.400363583713343 1103 20-21 0.450937451052404 1103 22-23 0.31826515578865866 1103 24-25 0.35241478730090847 1103 26-27 0.2486791926569225 1103 28-29 0.3341325129771988 1103 30-31 0.4707236717231993 1103 32-33 0.30561196500230636 1103 34-35 0.25548139772555345 1103 36-37 0.156959398627059 1103 38-39 0.04896261310863537 1103 40-41 -0.004544025785314432 1103 42-43 -0.13826879074886023 1103 44-45 -0.08757145759880558 1103 46-47 0.004422818859758593 1103 48-49 -0.04466446937516366 1103 50-51 -0.052973511682502306 1103 52-53 -0.07472386794999153 1103 54-55 -0.21150709529604228 1103 56-57 -0.15602415128991254 1103 58-59 -0.1369196186967585 1103 60-61 -0.11878705560788916 1103 62-63 -0.10572028731375127 1103 64-65 -0.30579478884887834 1103 66-67 -0.32153646914491674 1103 68-69 -0.43794232043826753 1103 70-71 -0.332278006342257 1103 72-73 -0.5288608350102422 1103 74-75 -0.46875159396137533 1103 76-77 -0.5957898458364497 1103 78-79 -0.5986837920035377 1103 80-81 -0.5944687268293798 1103 82-83 -0.6533879318424383 1103 84-85 -0.6463127033918248 1103 86-87 -0.7888768286488208 1103 88-89 -0.7427899848263522 1103 90-91 -0.8303439875208625 1103 92-93 -0.8460861329522658 1103 94-95 -0.7979037458332314 1103 96-97 -0.8683046065056033 1103 98-99 -0.7680277989218105 1103 100-101 -0.6677173982374391 1104 1 0.19048804515551154 1104 2 0.21696003002053033 1104 3 0.2518662185238867 1104 4 0.1712632156137559 1104 5 0.2418923989363293 1104 6 0.3561029071550763 1104 7 0.20016805838655216 1104 8 0.3071220252729816 1104 9 0.33638274629308995 1104 10-11 0.41467976706195486 1104 12-13 0.5286272785934827 1104 14-15 0.41949496119075036 1104 16-17 0.5002972409528468 1104 18-19 0.447861121637942 1104 20-21 0.37442197885226136 1104 22-23 0.3569526752261041 1104 24-25 0.3598236905111136 1104 26-27 0.5137723627733735 1104 28-29 0.3185679850045702 1104 30-31 0.2532732803827926 1104 32-33 0.41680436539623855 1104 34-35 0.4037534095435191 1104 36-37 0.509381195337383 1104 38-39 0.5674773121307197 1104 40-41 0.37948034444171697 1104 42-43 0.4096833672386353 1104 44-45 0.40422702368942254 1104 46-47 0.5157991190083422 1104 48-49 0.3931341112599682 1104 50-51 0.43492182419641523 1104 52-53 0.4382840970798938 1104 54-55 0.4169333311181376 1104 56-57 0.39695852347423966 1104 58-59 0.4882806948938949 1104 60-61 0.35939442094623786 1104 62-63 0.5166696967907498 1104 64-65 0.355665524223447 1104 66-67 0.3961671488100862 1104 68-69 0.3368927853013872 1104 70-71 0.35733797468680706 1104 72-73 0.25311806889106947 1104 74-75 0.3752521725820479 1104 76-77 0.33165721954244987 1104 78-79 0.4176879295486913 1104 80-81 0.21496879692749005 1104 82-83 0.15204332010130273 1104 84-85 0.3188570286307062 1104 86-87 0.08223838352160939 1104 88-89 0.22581261460693725 1104 90-91 0.053809425693138735 1104 92-93 0.05153094521214996 1104 94-95 0.11434810288303154 1104 96-97 0.16818054329224807 1104 98-99 0.20507510719104616 1104 100-101 0.13774624943524927 1105 1 0.1010316351777476 1105 2 0.12188140998809871 1105 3 0.2472189219495604 1105 4 0.251873304557634 1105 5 0.25139702167720657 1105 6 0.21166890033381947 1105 7 0.1854962897075012 1105 8 0.26044007486085974 1105 9 0.2541164538483045 1105 10-11 0.3138002550829526 1105 12-13 0.33018693117737286 1105 14-15 0.3489913708341348 1105 16-17 0.3566599881817112 1105 18-19 0.38223627837644614 1105 20-21 0.4110899063930944 1105 22-23 0.3433198215822486 1105 24-25 0.43062612894421903 1105 26-27 0.4125198131604151 1105 28-29 0.26538687262097227 1105 30-31 0.2485327243999791 1105 32-33 0.3235854476860496 1105 34-35 0.37556294509598587 1105 36-37 0.25286061243734537 1105 38-39 0.19954804717159647 1105 40-41 0.1839004746809607 1105 42-43 0.1751555471995161 1105 44-45 0.0909490782594915 1105 46-47 0.1841357473332934 1105 48-49 0.16731703345079296 1105 50-51 0.0841695316345934 1105 52-53 0.20374373796064305 1105 54-55 0.3092341269317487 1105 56-57 0.3084663165125505 1105 58-59 0.3419901205204283 1105 60-61 0.32223851420355487 1105 62-63 0.17473472864001138 1105 64-65 0.08906796182062493 1105 66-67 0.007787950308092206 1105 68-69 0.06817533466357162 1105 70-71 0.01881285263975485 1105 72-73 -0.04916676049384705 1105 74-75 -0.12006448098825118 1105 76-77 0.008811694740149534 1105 78-79 0.001094233362241681 1105 80-81 -0.0860725736775656 1105 82-83 0.0144122867139167 1105 84-85 -0.1665425187084999 1105 86-87 -0.10125511914503704 1105 88-89 -0.29746592642929315 1105 90-91 -0.5169776001206472 1105 92-93 -0.3060561909455437 1105 94-95 -0.4081569336474864 1105 96-97 -0.33447617431094656 1105 98-99 -0.32069897202250175 1105 100-101 -0.5374320144873082 1106 1 0.07760947182306666 1106 2 0.12311632375012493 1106 3 0.18122210244472114 1106 4 0.17496773683718914 1106 5 0.20294390823199393 1106 6 0.20528370611555857 1106 7 0.23105780983587465 1106 8 0.21262862219441558 1106 9 0.24547308746721086 1106 10-11 0.28268511456645484 1106 12-13 0.19442907109031893 1106 14-15 0.1978421491696949 1106 16-17 0.2576521419990314 1106 18-19 0.29584664506035097 1106 20-21 0.2507401137226495 1106 22-23 0.2519256461729924 1106 24-25 0.40183533507697433 1106 26-27 0.3175285265636063 1106 28-29 0.28420797800783504 1106 30-31 0.28207546436359365 1106 32-33 0.22765201149473313 1106 34-35 0.22972004178181038 1106 36-37 0.28748995291717705 1106 38-39 0.4698988903941981 1106 40-41 0.3699993522380751 1106 42-43 0.40468986420671627 1106 44-45 0.6226896577935079 1106 46-47 0.3104803511000327 1106 48-49 0.3305872581247584 1106 50-51 0.21188320055411936 1106 52-53 0.31830258845059234 1106 54-55 0.29665196242353176 1106 56-57 0.23426257325101574 1106 58-59 0.36185740076449235 1106 60-61 0.3815445009089231 1106 62-63 0.40455743251407483 1106 64-65 0.12743036729668233 1106 66-67 0.04386488986425974 1106 68-69 0.0915145867940339 1106 70-71 0.14409686220596285 1106 72-73 0.17434233090212814 1106 74-75 0.10282097380813937 1106 76-77 0.06128651753671832 1106 78-79 0.17582371618159698 1106 80-81 0.32862825584665956 1106 82-83 0.15269301690946335 1106 84-85 0.35664043803964773 1106 86-87 0.2658683145537921 1106 88-89 0.3221541135740864 1106 90-91 0.33046677726240503 1106 92-93 0.31965061436654096 1106 94-95 0.26794933897285844 1106 96-97 0.08585437883565916 1106 98-99 0.1439702894393271 1106 100-101 0.1430729303175049 1107 1 -0.012515500324987272 1107 2 0.0017307238877251052 1107 3 -0.04266743577986887 1107 4 0.08720578200228601 1107 5 0.14525227710505817 1107 6 0.17435241538820634 1107 7 0.006136283901319928 1107 8 0.039995450825820456 1107 9 0.054940401992830346 1107 10-11 0.1319782616207945 1107 12-13 0.13696074412221293 1107 14-15 0.18967029194362794 1107 16-17 0.1781304526664229 1107 18-19 0.25808967425700047 1107 20-21 0.0957934135424452 1107 22-23 0.19987232289893342 1107 24-25 0.21924875951710732 1107 26-27 0.22417224062482433 1107 28-29 0.09834318012497789 1107 30-31 0.2269672229253885 1107 32-33 0.1862415501102319 1107 34-35 0.06284098370513647 1107 36-37 0.2502264989637695 1107 38-39 0.2276153439527988 1107 40-41 -0.0207990505263993 1107 42-43 0.03937696475124852 1107 44-45 0.08422857037554365 1107 46-47 0.21766644506563892 1107 48-49 0.2913814977569942 1107 50-51 0.41556395671965873 1107 52-53 0.38782445369020024 1107 54-55 0.3675761973258247 1107 56-57 0.4262518663785215 1107 58-59 0.4634231684812562 1107 60-61 0.331356769053734 1107 62-63 0.4148352873869001 1107 64-65 0.2553768094799622 1107 66-67 0.26969184819775904 1107 68-69 0.37903750356656474 1107 70-71 0.38712605885825013 1107 72-73 0.3217611889139107 1107 74-75 0.39577550771323544 1107 76-77 0.3930700776597753 1107 78-79 0.35091372192789194 1107 80-81 0.28216437737103206 1107 82-83 0.28016841924785396 1107 84-85 0.3306956220188191 1107 86-87 0.6525407210005874 1107 88-89 0.5779390105903097 1107 90-91 0.45402189674234705 1107 92-93 0.5887599777004873 1107 94-95 0.544206878542159 1107 96-97 0.35360144021156614 1107 98-99 0.49524532198212157 1107 100-101 0.49481460503028885 1108 1 0.04931872859167896 1108 2 0.12177137316243858 1108 3 0.15764161007585642 1108 4 0.25670954530842494 1108 5 0.2756687001544904 1108 6 0.20443287867856696 1108 7 0.16885774356294547 1108 8 0.2196457556953817 1108 9 0.31435794800168537 1108 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-1.6721176428207656 2316 68-69 -1.703414657418577 2316 70-71 -1.5989349710905607 2316 72-73 -1.3507610720926984 2316 74-75 -1.314732724645431 2316 76-77 -1.4555820887996838 2316 78-79 -1.1715619353858635 2316 80-81 -1.093998013808985 2316 82-83 -1.2737535660262473 2316 84-85 -1.0798166440443246 2316 86-87 -1.014225587770845 2316 88-89 -1.1302793741703532 2316 90-91 -1.2145729046769382 2316 92-93 -1.2615672565756277 2316 94-95 -0.9309962833059124 2316 96-97 -1.0929692627046599 2316 98-99 -1.0397402215091347 2316 100-101 -0.9051293552895849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5185.0 3 1386.0 4 1444.0 5 1572.0 6 1876.0 7 2188.0 8 2630.0 9 3204.0 10 3809.0 11 4909.0 12 6431.0 13 9429.0 14 8675.0 15 9118.0 16 11669.0 17 15936.0 18 15013.0 19 9310.0 20 8929.0 21 9606.0 22 9886.0 23 10705.0 24 11692.0 25 13185.0 26 15046.0 27 17174.0 28 20068.0 29 24041.0 30 28613.0 31 34897.0 32 43280.0 33 56661.0 34 78684.0 35 118665.0 36 185603.0 37 309427.0 38 325909.0 39 63146.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5902681789734 19.004193538670584 14.564999849394061 21.84053843296195 2 15.003342788426538 20.602129366246054 41.19437396351852 23.200153881808884 3 19.606477322891756 25.705664752392067 29.871098683204362 24.81675924151182 4 12.483018777191663 16.81865723777452 37.74720452672313 32.95111945831068 5 13.252566979271114 38.24927705072769 33.742108346532014 14.756047623469183 6 30.656290019109022 36.289177831399954 18.055754005082385 14.998778144408643 7 27.929001115784512 33.69255228862694 20.678338222263474 17.700108373325072 8 26.03217595003993 34.94613747031751 19.783160914474372 19.238525665168186 9 26.82663661165335 17.276513689675756 20.773146522196065 35.12370317647483 10-11 24.782868512595897 27.134558759706483 26.982560041663607 21.100012686034013 12-13 26.387327651337472 24.572756934259537 27.68939698708881 21.350518427314178 14-15 23.079360246361105 25.56517291550233 27.004538476895966 24.35092836124059 16-17 21.460205720420387 29.056265165897265 27.548797093355333 21.934732020327015 18-19 21.269989339464114 27.4695934339379 30.38960535984901 20.87081186674898 20-21 22.528812545160466 26.27197252907967 30.194138254805875 21.00507667095399 22-23 21.97285637816342 26.471526178404716 29.970250408810607 21.585367034621257 24-25 21.832878515790814 26.498635213397986 30.288376920895814 21.380109349915386 26-27 21.77806707068224 26.456563427395086 30.47710396576933 21.28826553615334 28-29 21.231690729492165 27.01401558147682 30.569690023520156 21.184603665510863 30-31 21.467752540236905 26.716924327336855 30.343563514354187 21.471759618072053 32-33 21.343707886628298 26.731581639671813 30.246533097019856 21.678177376680026 34-35 22.01680711638687 26.692808987711235 29.991386285923106 21.29899760997879 36-37 21.949663736652706 26.52870439394833 30.163009959187175 21.358621910211788 38-39 21.755552010894917 26.653774080607366 30.2864741111689 21.304199797328817 40-41 21.765729316350622 26.706962467011596 30.238635522241143 21.288672694396638 42-43 21.475912659259578 26.94878490841234 29.736832932950108 21.83846949937798 44-45 21.64024152152528 27.004425883626578 29.493847167082553 21.86148542776559 46-47 21.884246017686657 27.218633636557744 28.97965695944096 21.917463386314644 48-49 21.99335788731606 27.24348971384341 28.938411786179884 21.82474061266064 50-51 21.885267166719512 27.52215747173209 28.887269567992146 21.70530579355625 52-53 22.063675573085856 27.652324841223365 28.6128786479575 21.671120937733278 54-55 21.53811812945112 27.394886614053416 28.908430889722986 22.158564366772477 56-57 21.88639186733554 27.76325925816713 28.465029281465636 21.885319593031692 58-59 22.076344450217913 27.27387242333879 28.634043335849313 22.015739790593983 60-61 21.917944465802492 27.26038838306782 29.153879745114402 21.66778740601529 62-63 21.905193089999642 27.278140952666917 29.036505415675418 21.780160541658024 64-65 21.703730272596843 27.40268708349535 28.864291556604403 22.029291087303395 66-67 21.593435973080112 27.531768314657594 28.687077281527362 22.187718430734932 68-69 21.53384754397641 27.520540206164828 28.812951268455173 22.13266098140359 70-71 21.650578788138866 27.514697678410506 28.54279760116723 22.291925932283398 72-73 21.739889809101896 27.380611162234057 28.558617903758122 22.320881124905924 74-75 21.56236149842196 27.82801364347406 28.317986548290914 22.29163830981307 76-77 21.69488469000173 27.425190283954475 28.46235012247623 22.417574903567562 78-79 21.733442223340017 27.398191250752753 28.583124081994576 22.285242443912654 80-81 21.762520492709754 27.761639792117126 28.294684831172244 22.181154884000872 82-83 21.826807703393865 27.614139870358827 28.284198389875385 22.274854036371924 84-85 21.77163091329871 27.545527519284363 28.189507094534015 22.493334472882907 86-87 21.77706362916649 27.439227539679827 28.385530312323827 22.39817851882986 88-89 21.87578879408741 27.293205635706443 28.178331070098384 22.652674500107768 90-91 21.780386082781835 27.37952514097587 28.380269413097846 22.459819363144447 92-93 21.921185403711092 27.37659028813758 28.317135721349672 22.385088586801654 94-95 21.783683517267484 27.16797732110212 28.35398632386826 22.694352837762136 96-97 21.62556757380136 27.064755041449985 28.40145336440853 22.90822402034013 98-99 21.762666500392555 27.12869415150924 28.02017146347367 23.08846788462454 100-101 22.052708761050823 26.47742908618595 28.648761888800433 22.821100263962794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2735.0 1 1681.0 2 812.0 3 955.0 4 730.0 5 664.5 6 1140.5 7 2317.0 8 3703.5 9 4196.5 10 3493.5 11 2769.0 12 2775.5 13 2924.0 14 3072.0 15 3216.0 16 3361.0 17 3500.5 18 3528.0 19 3604.5 20 3872.5 21 4330.5 22 4969.0 23 5805.0 24 6716.5 25 7959.0 26 9809.0 27 12001.0 28 13695.0 29 15648.5 30 18327.0 31 20726.5 32 23216.5 33 25774.0 34 28533.0 35 31200.5 36 34394.0 37 37762.0 38 39885.0 39 41459.0 40 42887.0 41 45309.5 42 47765.0 43 49294.0 44 51648.5 45 54156.0 46 54878.0 47 57609.5 48 68473.5 49 68443.5 50 58101.0 51 53503.5 52 49971.0 53 48297.5 54 46275.5 55 42019.0 56 37222.0 57 32842.5 58 28964.0 59 25098.5 60 21063.0 61 17380.5 62 14350.5 63 11398.0 64 8521.5 65 6503.5 66 5031.0 67 3953.0 68 2994.0 69 2087.0 70 1543.0 71 1222.5 72 988.0 73 834.0 74 665.0 75 543.5 76 416.5 77 263.0 78 165.5 79 123.5 80 86.0 81 59.5 82 43.5 83 34.5 84 26.5 85 19.0 86 14.0 87 11.0 88 11.0 89 6.0 90 4.0 91 7.0 92 4.5 93 2.0 94 2.0 95 3.0 96 3.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33855000533674884 2 0.11920962749493008 3 0.11293894759312627 4 0.11994343046216245 5 0.13655406126587682 6 0.08792293734656847 7 0.09599476998612445 8 0.10166506564201089 9 0.08952396200234818 10-11 0.08872344967445832 12-13 0.09242581919094887 14-15 0.09345981428113993 16-17 0.08208586828903831 18-19 0.09742902124026044 20-21 0.1068683957732949 22-23 0.11397294268331733 24-25 0.12804861778204718 26-27 0.12694791333119862 28-29 0.12097742555235351 30-31 0.11270546483082505 32-33 0.10633472088803501 34-35 0.3276096701889209 36-37 0.4904472195538478 38-39 0.46483082506137263 40-41 0.44425098729853774 42-43 0.47603799765183047 44-45 0.4834760913651403 46-47 0.4903471555128615 48-49 0.5000867221688547 50-51 0.4890463229800406 52-53 0.49368262354573594 54-55 0.48100784502081334 56-57 0.4592605934464724 58-59 0.43898094780659624 60-61 0.3987885580104601 62-63 0.46886674138115064 64-65 0.49765183050485645 66-67 0.5054901803821111 68-69 0.5174978653004589 70-71 0.5101931903084641 72-73 0.5028218059558117 74-75 0.5090591311772868 76-77 0.4884459387341232 78-79 0.4679328103319458 80-81 0.4700341551926566 82-83 0.4823086775536343 84-85 0.4818083573487032 86-87 0.4861111111111111 88-89 0.49655112605400786 90-91 0.510326609029779 92-93 0.5046563133738926 94-95 0.5076248799231509 96-97 0.5072579784395346 98-99 0.5021213576689081 100-101 0.4904805742341765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499040.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.74924197677951 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58103511326686 48.34487558858436 2 12.357437155403124 15.014098799328607 3 3.660046041274176 6.6703506782255655 4 1.5661793860886883 3.805768420181828 5 0.782910388781744 2.3780606327118345 6 0.4719964763498378 1.7204056892378152 7 0.2901991856556803 1.2340566385602898 8 0.21234037514955606 1.0319613465120414 9 0.14833925467998504 0.811034754948858 >10 0.8312403685716039 9.348563945234229 >50 0.06555253434781325 2.7541291598217406 >100 0.029087867285258077 3.2968679682155937 >500 0.002203547360978874 0.9116796267422305 >1k 0.0012119510485383805 1.577919917806922 >5k 2.203547360978874E-4 1.1002268338880248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9248 0.616928167360444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7190 0.4796403031273348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4909 0.32747625146760595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3383 0.22567776710428009 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2295 0.1530979827089337 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2194 0.1463603372825275 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2095 0.13975611057743623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1716 0.11447326288824848 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1664 0.11100437613405913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.670936065748746E-5 0.0 5 0.0 0.0 0.0 6.670936065748746E-5 0.0 6 0.0 0.0 0.0 6.670936065748746E-5 0.0 7 0.0 0.0 0.0 6.670936065748746E-5 0.0 8 0.0 0.0 0.0 6.670936065748746E-5 0.0 9 0.0 0.0 0.0 6.670936065748746E-5 0.0 10-11 0.0 0.0 0.0 6.670936065748746E-5 0.0 12-13 0.0 0.0 0.0 6.670936065748746E-5 0.0 14-15 0.0 0.0 0.0 6.670936065748746E-5 0.0 16-17 0.0 0.0 0.0 6.670936065748746E-5 0.0 18-19 0.0 0.0 0.0 1.000640409862312E-4 0.0 20-21 0.0 0.0 0.0 1.3341872131497493E-4 0.0 22-23 0.0 0.0 0.0 3.3354680328743727E-4 0.0 24-25 0.0 0.0 0.0 7.671576475611057E-4 0.0 26-27 0.0 0.0 0.0 0.0013341872131497493 0.0 28-29 0.0 0.0 0.0 0.002735083786956986 0.0 30-31 0.0 0.0 0.0 0.008838990287117088 0.0 32-33 0.0 0.0 0.0 0.01984603479560252 0.0 34-35 0.0 0.0 0.0 0.035289251787810866 0.0 36-37 0.0 0.0 0.0 0.06050539011634112 0.0 38-39 0.0 0.0 0.0 0.10133151883872345 0.0 40-41 0.0 0.0 0.0 0.14565988899562388 0.0 42-43 0.0 0.0 0.0 0.18918774682463443 0.0 44-45 0.0 0.0 0.0 0.245990767424485 0.0 46-47 0.0 0.0 0.0 0.3135006404098623 0.0 48-49 0.0 0.0 0.0 0.3957199274202156 0.0 50-51 0.0 0.0 0.0 0.4851104707012488 0.0 52-53 0.0 0.0 0.0 0.5704650976625041 0.0 54-55 0.0 6.670936065748746E-5 0.0 0.6694617888782154 0.0 56-57 0.0 6.670936065748746E-5 0.0 0.7866034261927634 0.0 58-59 0.0 6.670936065748746E-5 0.0 0.9267931476144733 0.0 60-61 0.0 6.670936065748746E-5 0.0 1.0817589924218165 0.0 62-63 0.0 6.670936065748746E-5 0.0 1.237892251040666 0.0 64-65 0.0 6.670936065748746E-5 0.0 1.4092018892090938 0.0 66-67 6.670936065748746E-5 6.670936065748746E-5 0.0 1.6000240153698368 0.0 68-69 6.670936065748746E-5 6.670936065748746E-5 0.0 1.8251681075888568 0.0 70-71 6.670936065748746E-5 6.670936065748746E-5 0.0 2.0756284021773936 0.0 72-73 6.670936065748746E-5 6.670936065748746E-5 0.0 2.338096381684278 0.0 74-75 6.670936065748746E-5 6.670936065748746E-5 0.0 2.6022988045682567 0.0 76-77 6.670936065748746E-5 6.670936065748746E-5 0.0 2.893351745116875 0.0 78-79 6.670936065748746E-5 6.670936065748746E-5 0.0 3.2345034155192653 0.0 80-81 6.670936065748746E-5 6.670936065748746E-5 0.0 3.604840431209307 0.0 82-83 6.670936065748746E-5 6.670936065748746E-5 0.0 3.992221688547337 0.0 84-85 6.670936065748746E-5 6.670936065748746E-5 0.0 4.386574074074074 0.0 86-87 6.670936065748746E-5 6.670936065748746E-5 0.0 4.793534528765076 0.0 88-89 6.670936065748746E-5 6.670936065748746E-5 0.0 5.238052353506244 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1440 0.0 25.00518 2 GTATCAA 13550 0.0 23.227127 1 GTACATG 8480 0.0 22.839447 1 GAGTACT 6685 0.0 22.598497 12-13 TACATGG 8615 0.0 22.43798 2 GTACCTG 1720 0.0 21.527193 1 AGTACTT 6755 0.0 21.452917 12-13 GGTATCA 10170 0.0 21.237907 1 ACATGGG 8710 0.0 21.214136 3 GTACTTT 7245 0.0 20.819767 14-15 ATAACGC 140 4.937398E-4 20.304958 3 TCAACGC 15645 0.0 20.047552 4 ATCAACG 15635 0.0 19.999767 3 TATCAAC 15710 0.0 19.934446 2 GTACACG 215 1.0857475E-6 19.871256 1 CAACGCA 15795 0.0 19.82717 5 AACGCAG 16140 0.0 19.453615 6 GTGTAGC 830 0.0 19.445635 1 ATGGGAT 2435 0.0 19.068037 5 CATGGGA 5740 0.0 18.241446 4 >>END_MODULE