FastQCFastQC Report
Fri 27 May 2016
SRR939213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499040
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90490.603653004589604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78990.5269372398334934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53010.353626320845341No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37870.25262834880990503No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24370.16257071192229694No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21850.1457599530366101No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18880.12594727292133634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.12588056356067884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17530.11694150923257551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13400.025.1385292
GTACCTG14550.023.8858571
CATGGGG30700.022.408664
GAGTACT76050.022.39955912-13
GTACATG90100.022.3509791
TACATGG90750.022.0623662
ACATGGG90350.021.2148283
GTATCAA155200.021.0432761
GGTATCA121650.021.0156651
AGTACTT78250.020.86019512-13
GTACTTT81250.020.81998614-15
GTATTAG5000.019.0432431
TACTTTT88150.018.40975614-15
ACTTTTT92900.018.33555416-17
GTCTTAG6000.018.2497771
ATCAACG180500.018.0315573
CAACGCA181300.017.978165
TCAACGC181400.017.9420954
TATCAAC181650.017.91742
AACGCAG185400.017.5805856
Produced by FastQC (version 0.11.5)