##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499040 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.505169975450954 34.0 31.0 34.0 31.0 34.0 2 32.710331278685025 34.0 31.0 34.0 31.0 34.0 3 32.793500506991144 34.0 31.0 34.0 31.0 34.0 4 36.24534902337496 37.0 35.0 37.0 35.0 37.0 5 36.215157033834984 37.0 37.0 37.0 35.0 37.0 6 36.262407941082294 37.0 37.0 37.0 35.0 37.0 7 36.216814761447324 37.0 37.0 37.0 35.0 37.0 8 36.20966752054648 37.0 37.0 37.0 35.0 37.0 9 37.95174311559398 39.0 38.0 39.0 35.0 39.0 10-11 37.896148201515636 39.0 38.0 39.0 35.0 39.0 12-13 37.832057183263956 39.0 38.0 39.0 35.0 39.0 14-15 39.17198807236632 40.5 39.0 41.0 36.0 41.0 16-17 39.12038738125734 40.0 38.5 41.0 36.0 41.0 18-19 39.1793217659302 40.5 38.5 41.0 36.0 41.0 20-21 39.25464530632938 41.0 39.0 41.0 36.0 41.0 22-23 39.21245463763475 41.0 39.0 41.0 36.0 41.0 24-25 39.1566766063614 41.0 39.0 41.0 35.0 41.0 26-27 39.01277484256591 40.5 39.0 41.0 35.0 41.0 28-29 38.86018451809158 40.0 38.0 41.0 35.0 41.0 30-31 38.65365533941723 40.0 38.0 41.0 35.0 41.0 32-33 38.47774342245704 40.0 38.0 41.0 34.0 41.0 34-35 38.39611684811612 40.0 38.0 41.0 34.0 41.0 36-37 38.27159682196606 40.0 38.0 41.0 34.0 41.0 38-39 38.140563293841396 40.0 38.0 41.0 34.0 41.0 40-41 37.96475177446899 40.0 38.0 41.0 33.0 41.0 42-43 37.782205278044614 40.0 37.0 41.0 33.0 41.0 44-45 37.521671869996794 40.0 37.0 41.0 33.0 41.0 46-47 37.15860684171203 40.0 37.0 41.0 32.0 41.0 48-49 36.789123705838406 40.0 36.5 41.0 31.0 41.0 50-51 36.516241060945674 40.0 36.0 41.0 30.5 41.0 52-53 36.281355400789835 39.5 36.0 41.0 30.0 41.0 54-55 36.0477682383392 39.0 35.0 41.0 29.5 41.0 56-57 35.79313327196073 39.0 35.0 41.0 28.5 41.0 58-59 35.5052460241221 39.0 35.0 40.0 28.0 41.0 60-61 35.16225884566123 38.0 35.0 40.0 28.0 41.0 62-63 34.775207799658446 38.0 34.0 40.0 26.5 41.0 64-65 34.37334127174725 37.0 34.0 40.0 26.0 41.0 66-67 34.01270746611164 37.0 34.0 39.5 26.0 41.0 68-69 33.55702949887929 36.0 33.5 39.0 25.5 40.5 70-71 33.1262678113993 36.0 33.0 39.0 24.0 40.0 72-73 32.659226571672534 35.0 32.5 38.0 22.5 40.0 74-75 32.17608202582987 35.0 32.0 37.0 21.5 39.0 76-77 30.192845754616286 33.5 29.5 35.5 18.0 37.5 78-79 31.14845000800512 34.5 31.0 36.0 18.5 39.0 80-81 31.17385259899669 35.0 31.0 36.0 18.5 37.5 82-83 30.87470747945352 35.0 31.0 35.5 17.5 37.0 84-85 30.493861071085494 34.5 31.0 35.0 12.5 37.0 86-87 29.94706345394386 34.0 30.5 35.0 4.5 36.0 88-89 29.405534208560148 34.0 30.0 35.0 2.0 36.0 90-91 28.953208053154018 34.0 29.0 35.0 2.0 35.5 92-93 28.680947806596222 34.0 29.0 35.0 2.0 35.0 94-95 28.368281033194577 34.0 29.0 35.0 2.0 35.0 96-97 27.832368048884618 34.0 29.0 35.0 2.0 35.0 98-99 27.089219433770946 34.0 27.0 35.0 2.0 35.0 100-101 23.576142064254455 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18684972094582974 1101 2 0.18416652405549883 1101 3 0.13880884759133494 1101 4 0.1034186658777898 1101 5 0.06658016010523227 1101 6 0.1141282594011983 1101 7 0.06095562802090626 1101 8 0.1098220107209471 1101 9 0.31012573699310053 1101 10-11 0.19755750291755447 1101 12-13 0.22204370627223824 1101 14-15 0.24848878668004204 1101 16-17 0.2715804875547789 1101 18-19 0.2415765694178731 1101 20-21 0.20903313978360671 1101 22-23 0.16025414514741243 1101 24-25 0.11486995322896121 1101 26-27 0.08595228702910873 1101 28-29 0.13221459625252407 1101 30-31 0.17781412769641491 1101 32-33 0.21600451226251494 1101 34-35 0.3209046815254837 1101 36-37 0.41631848021025775 1101 38-39 0.45232883964758486 1101 40-41 0.41812777710227067 1101 42-43 0.3977491844786414 1101 44-45 0.41493711963688185 1101 46-47 0.23814926056588348 1101 48-49 0.33182002561553503 1101 50-51 0.3567496748575323 1101 52-53 0.19281833805933246 1101 54-55 0.22221741829201136 1101 56-57 0.2807774987427294 1101 58-59 0.10770919949029434 1101 60-61 0.09948237610408484 1101 62-63 -0.036571888985697854 1101 64-65 0.0156165209864767 1101 66-67 0.11864443618639342 1101 68-69 0.15981139623631435 1101 70-71 0.08965772177042908 1101 72-73 0.052602624358684125 1101 74-75 0.0860092602595941 1101 76-77 0.10500255879473741 1101 78-79 0.3621488322167927 1101 80-81 0.42073833841184083 1101 82-83 0.3869190558802238 1101 84-85 0.31992334163549074 1101 86-87 0.32286166933580773 1101 88-89 0.5147997709716599 1101 90-91 0.576709990471791 1101 92-93 0.41265562744125717 1101 94-95 0.38853977899887937 1101 96-97 0.6165950764635362 1101 98-99 0.6914866742053363 1101 100-101 0.2125165244007512 1102 1 0.2440539261257939 1102 2 0.192573196852031 1102 3 0.218653227357251 1102 4 0.14223961158239007 1102 5 0.12112847427697915 1102 6 0.13604829000443175 1102 7 0.19634212065469825 1102 8 0.10884491936521101 1102 9 0.27038300699063456 1102 10-11 0.19154718123787973 1102 12-13 0.14988268668663096 1102 14-15 0.1892292905734294 1102 16-17 0.2176235556216355 1102 18-19 0.21413274640967472 1102 20-21 0.2514715127421425 1102 22-23 0.30376789471244336 1102 24-25 0.16419491906533068 1102 26-27 0.15718288058906182 1102 28-29 0.161113398163387 1102 30-31 0.09825665329321254 1102 32-33 0.15173425432421794 1102 34-35 0.13023936066390007 1102 36-37 0.267444843010729 1102 38-39 0.19899514912283678 1102 40-41 0.25711798544856634 1102 42-43 0.30893584349449554 1102 44-45 0.31083108909355417 1102 46-47 0.4901616309111674 1102 48-49 0.33060557480309427 1102 50-51 0.460793196903154 1102 52-53 0.4858803557068967 1102 54-55 0.33614880527779434 1102 56-57 0.28589426576866117 1102 58-59 0.47992483479554693 1102 60-61 0.5267867216257045 1102 62-63 0.32980136221665646 1102 64-65 0.23931311798929045 1102 66-67 0.3083608953363708 1102 68-69 0.41513930947797917 1102 70-71 0.2898272148581924 1102 72-73 0.11757948757280445 1102 74-75 0.20627820235080208 1102 76-77 0.19846947019513905 1102 78-79 0.29319619810293673 1102 80-81 0.3062950730831453 1102 82-83 0.2640941787655926 1102 84-85 0.26328977854742064 1102 86-87 0.4056877332477242 1102 88-89 0.28902138174363046 1102 90-91 0.47245993113192597 1102 92-93 0.22847131436643764 1102 94-95 0.35443808084175643 1102 96-97 0.2686500217210721 1102 98-99 0.33092498494343303 1102 100-101 -0.24096419071950592 1103 1 -0.02409765200881253 1103 2 -0.04908497797951128 1103 3 0.030281504943744153 1103 4 0.011748910702984006 1103 5 0.11697271232368678 1103 6 0.12868845017465702 1103 7 0.1307241769207792 1103 8 0.10420545949081372 1103 9 0.07034529875362949 1103 10-11 0.17198210535005387 1103 12-13 0.12226420875267507 1103 14-15 0.1726752623012331 1103 16-17 0.10022580554644378 1103 18-19 0.13406446756624035 1103 20-21 0.1770920152492934 1103 22-23 0.12501738408006702 1103 24-25 0.021300340522216743 1103 26-27 0.012362488513176118 1103 28-29 0.09310558268235525 1103 30-31 0.19043309192979052 1103 32-33 0.20870174892610294 1103 34-35 0.13423014908028819 1103 36-37 0.18721776310966476 1103 38-39 0.15392426661541236 1103 40-41 0.19726289281648945 1103 42-43 0.294616813853132 1103 44-45 0.21206973734303602 1103 46-47 0.3304885703041478 1103 48-49 0.4725916766243188 1103 50-51 0.40134773244091804 1103 52-53 0.35603664288206005 1103 54-55 0.4018711200248575 1103 56-57 0.3802237774021009 1103 58-59 0.4610622992757385 1103 60-61 0.45873962673113766 1103 62-63 0.36568759556779895 1103 64-65 0.28182410500239286 1103 66-67 0.27671739978279675 1103 68-69 0.38174507434916194 1103 70-71 0.22089296635193278 1103 72-73 0.24291167918451606 1103 74-75 0.34531105340775525 1103 76-77 0.4502566835115296 1103 78-79 0.43522775181697426 1103 80-81 0.378430593793496 1103 82-83 0.27763772055664404 1103 84-85 0.28729679497294924 1103 86-87 0.41633862679826805 1103 88-89 0.11189254975626994 1103 90-91 0.2627342361107594 1103 92-93 0.17316533895759534 1103 94-95 0.15746921707174266 1103 96-97 0.3050581101379102 1103 98-99 0.3113615924590434 1103 100-101 0.14809222798557542 1104 1 0.050724603425024384 1104 2 0.10687885974812161 1104 3 0.007682244430249341 1104 4 -0.00850381917710763 1104 5 0.07247393904195576 1104 6 0.045167941518521104 1104 7 0.0480621550817375 1104 8 0.10512117876238136 1104 9 0.06659603305681827 1104 10-11 0.048002030883075975 1104 12-13 0.1524899637891295 1104 14-15 0.1461647574461935 1104 16-17 0.17623536444619958 1104 18-19 0.23236282791041418 1104 20-21 0.24896193991842352 1104 22-23 0.2576822135489536 1104 24-25 0.06511070456382129 1104 26-27 0.12341342755737372 1104 28-29 0.11499807637598991 1104 30-31 0.0846669761344927 1104 32-33 0.09030021898381335 1104 34-35 0.16777646633381949 1104 36-37 0.1626531617067215 1104 38-39 0.14014204366369398 1104 40-41 0.24706322714040851 1104 42-43 0.0940105817787682 1104 44-45 0.07868572269152452 1104 46-47 -0.05086099256948273 1104 48-49 -0.07300531186872661 1104 50-51 -0.12052640367297585 1104 52-53 -0.046592594479285765 1104 54-55 0.0181663876567697 1104 56-57 -0.014372938966808135 1104 58-59 -0.064843485305623 1104 60-61 -0.07329758948228005 1104 62-63 -0.2539476717185707 1104 64-65 -0.2384739788982202 1104 66-67 -0.12305399435660291 1104 68-69 -0.1005296025574367 1104 70-71 -0.0010966065427808758 1104 72-73 -0.20383136221290243 1104 74-75 -0.2158218468306785 1104 76-77 -0.14372494595044216 1104 78-79 0.01515287448880187 1104 80-81 0.07000111713569623 1104 82-83 0.006031929064196362 1104 84-85 2.1826340655195509E-4 1104 86-87 0.09161787798178977 1104 88-89 0.08409269488913651 1104 90-91 0.34696043172912994 1104 92-93 0.2819869119214182 1104 94-95 0.29278887652784036 1104 96-97 0.303443722978713 1104 98-99 0.393580872669137 1104 100-101 0.14017471183090535 1105 1 0.033726312919618806 1105 2 0.06623447642007818 1105 3 0.0204721061659896 1105 4 0.07156081978097717 1105 5 0.08149789704440025 1105 6 -0.013573469211586087 1105 7 0.05659447665062345 1105 8 0.08795099836682141 1105 9 0.10216522370099312 1105 10-11 0.1476148345377979 1105 12-13 0.048494229166230696 1105 14-15 0.11860072454364712 1105 16-17 0.0562888749706687 1105 18-19 0.1715467218799489 1105 20-21 0.20519375825222852 1105 22-23 0.1752116385903335 1105 24-25 0.15604124014973308 1105 26-27 0.16078746204819794 1105 28-29 0.34036374730036556 1105 30-31 0.35233009911779334 1105 32-33 0.2558758986019569 1105 34-35 0.43041246310709624 1105 36-37 0.41766252421988526 1105 38-39 0.4568024059595146 1105 40-41 0.43399034791682567 1105 42-43 0.4940767254264671 1105 44-45 0.36785362933390076 1105 46-47 0.15877361863544337 1105 48-49 0.23760807903292402 1105 50-51 0.28064448518062335 1105 52-53 0.26399728564710045 1105 54-55 0.23582757753188588 1105 56-57 0.26379845562967574 1105 58-59 0.4625220121614788 1105 60-61 0.4570323555129576 1105 62-63 0.43385640097466194 1105 64-65 0.18718338237774645 1105 66-67 0.02968062228192281 1105 68-69 0.08525698438854334 1105 70-71 0.1695367632932161 1105 72-73 0.014243356075766656 1105 74-75 0.025448863550003864 1105 76-77 0.055232899751995745 1105 78-79 0.20335532420708802 1105 80-81 0.14651223303023642 1105 82-83 0.09550662520048903 1105 84-85 0.17997825496357223 1105 86-87 0.220994539409908 1105 88-89 0.36090124821366487 1105 90-91 0.5519814451375851 1105 92-93 0.37669353924323445 1105 94-95 0.20225697919782704 1105 96-97 0.052482915963516064 1105 98-99 -0.11516565818535796 1105 100-101 -0.23479185062207364 1106 1 0.04533336933427279 1106 2 -0.0017995903085221698 1106 3 0.057625604195692404 1106 4 0.03888864767169764 1106 5 0.06822592145017126 1106 6 0.0786356565848223 1106 7 0.07563470455864518 1106 8 0.07968304988919073 1106 9 0.08113924622400503 1106 10-11 0.12052964299741831 1106 12-13 0.213943577102917 1106 14-15 0.19729506330345004 1106 16-17 0.06809728721557207 1106 18-19 0.09927285323193757 1106 20-21 0.16621930314891387 1106 22-23 0.16232254805951385 1106 24-25 0.17958957275866538 1106 26-27 0.21306221645588863 1106 28-29 0.15008274519720288 1106 30-31 0.20459954926703716 1106 32-33 0.20760276946025868 1106 34-35 0.15292610483584923 1106 36-37 0.23091297379441045 1106 38-39 0.24135939223435798 1106 40-41 0.13616602083668283 1106 42-43 0.1759036150299167 1106 44-45 0.3104025881543109 1106 46-47 0.19541574495301006 1106 48-49 0.2408086582785387 1106 50-51 0.19301581829011383 1106 52-53 0.20057122906972324 1106 54-55 0.11643927697509326 1106 56-57 0.17706584793263147 1106 58-59 0.041594343707508585 1106 60-61 0.06489625339104776 1106 62-63 0.18825430069430382 1106 64-65 0.0947059537998527 1106 66-67 0.01825818495971987 1106 68-69 -0.1321159039501154 1106 70-71 0.018221461692043306 1106 72-73 -0.15312273457246306 1106 74-75 -0.022070597413737403 1106 76-77 0.06269851476789157 1106 78-79 0.15392040797317463 1106 80-81 -0.13207437263574207 1106 82-83 -0.0439114307012467 1106 84-85 0.155825854863874 1106 86-87 0.1836311884369124 1106 88-89 0.42379250164598403 1106 90-91 0.653010316782769 1106 92-93 0.4338368819354095 1106 94-95 0.4777890014695352 1106 96-97 0.6857632238782934 1106 98-99 0.5471946029283536 1106 100-101 0.26366708205813083 1107 1 -0.03308694675105528 1107 2 -0.035834662254480065 1107 3 0.05116395387534567 1107 4 0.02984507355425592 1107 5 0.03994594005722263 1107 6 0.020436826952703768 1107 7 0.0059139666874372665 1107 8 0.015556154491306984 1107 9 0.09105746901607858 1107 10-11 0.06496550926119227 1107 12-13 0.0508880530368927 1107 14-15 0.08542163425065752 1107 16-17 0.13014163923283206 1107 18-19 0.11396499644368419 1107 20-21 0.03423530677650888 1107 22-23 0.048405666069648134 1107 24-25 0.0442418437179839 1107 26-27 0.08652645065455289 1107 28-29 0.06684111152938499 1107 30-31 0.10541487819357087 1107 32-33 0.12535794210320006 1107 34-35 0.181732829955763 1107 36-37 0.11818395265820669 1107 38-39 0.28601338317319147 1107 40-41 0.20312990517587792 1107 42-43 0.23176776792045928 1107 44-45 0.2668817314481515 1107 46-47 0.22820480901051354 1107 48-49 0.27889056839397597 1107 50-51 0.24521182168316358 1107 52-53 0.16669740520344334 1107 54-55 0.10077231582708635 1107 56-57 0.240482763149366 1107 58-59 0.14790715716399916 1107 60-61 0.07072268400354886 1107 62-63 0.06556992084465207 1107 64-65 0.22164630569202615 1107 66-67 0.23248700868918348 1107 68-69 0.11098121234785907 1107 70-71 0.0426319778031754 1107 72-73 0.06291597669378746 1107 74-75 -0.006309330570651639 1107 76-77 0.02305689595444349 1107 78-79 0.19607896272129466 1107 80-81 0.047191958374316556 1107 82-83 0.0986348035208735 1107 84-85 0.03451354958314212 1107 86-87 0.059065092360420124 1107 88-89 0.00956621879535291 1107 90-91 0.23618647395762693 1107 92-93 0.14687198124468281 1107 94-95 0.011015878494905706 1107 96-97 -0.014099861843138939 1107 98-99 0.2370334330295556 1107 100-101 -0.21962810551736212 1108 1 0.09754571559082592 1108 2 0.030986991372863315 1108 3 0.09168793973162792 1108 4 0.07466043489661445 1108 5 0.04398952201933781 1108 6 0.09720084770425075 1108 7 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94-95 -0.5528877246116224 2316 96-97 -0.9348631395737748 2316 98-99 -0.770242001277964 2316 100-101 -0.1502743193814915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 7.0 5 45.0 6 79.0 7 175.0 8 339.0 9 714.0 10 1232.0 11 1847.0 12 2867.0 13 4283.0 14 5384.0 15 7438.0 16 10318.0 17 15494.0 18 15244.0 19 9404.0 20 7850.0 21 7857.0 22 8041.0 23 8772.0 24 9779.0 25 11319.0 26 13616.0 27 16177.0 28 19333.0 29 23871.0 30 28928.0 31 35815.0 32 45104.0 33 58190.0 34 81528.0 35 121165.0 36 193421.0 37 344909.0 38 349224.0 39 39259.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68227518558114 19.55682775914919 15.065258822844235 21.695638232425438 2 15.321605827729748 21.1395292987512 40.53347475717793 23.005390116341125 3 19.704944497811933 25.687239833493436 30.133085174511688 24.474730494182946 4 12.667774042053582 17.389329170669228 37.36578076635714 32.57711602092005 5 13.142411143131605 38.500907247304944 33.65874159462056 14.697940014942898 6 30.585774895933397 36.61663464617355 17.74235510726865 15.055235350624399 7 27.807663571352332 33.89315828797097 20.54881791012915 17.750360230547553 8 26.028658341338456 35.237418614579994 19.523561746184225 19.21036129789732 9 26.5898841925499 17.433223929981857 20.650683103853133 35.326208773615114 10-11 24.832526150069377 27.28713043014196 26.78974543707973 21.090597982708932 12-13 26.335621464403886 24.74396947379656 27.66697352972569 21.253435532073862 14-15 22.91660621076412 25.781232019580706 27.011930743052243 24.29023102660293 16-17 21.326523056232485 29.245888450302832 27.54169588041166 21.885892613053024 18-19 21.100571032127228 27.574481001174085 30.495283648201514 20.82966431849717 20-21 22.391463870210266 26.328717045575832 30.303761073753872 20.976058010460026 22-23 21.845447753228733 26.51763795495784 30.166940175045365 21.469974116768064 24-25 21.740760469488862 26.56092971842876 30.490525735064804 21.207784077017575 26-27 21.636499785693676 26.591662233221985 30.6430357116054 21.128802269478943 28-29 21.15917520546483 27.06038531326716 30.687540025616393 21.09289945565162 30-31 21.40091007520362 26.70762516536057 30.51517114443858 21.376293614997227 32-33 21.320244956772335 26.787510673497707 30.348289571992744 21.543954797737218 34-35 21.901314553896643 26.727539048492083 30.185102345194576 21.186044052416698 36-37 21.864180407698335 26.4922174840441 30.40217645529929 21.241425652958277 38-39 21.650155217078716 26.64495907306796 30.525903153217023 21.178982556636303 40-41 21.632478775040447 26.742481610594965 30.403983120110755 21.22105649425383 42-43 21.430465705790773 26.969584865454138 29.800907413591055 21.799042015164037 44-45 21.60077705292272 27.022302246101894 29.60312795822807 21.773792742747318 46-47 21.862803534696372 27.26961616063236 29.06691714050228 21.800663164168988 48-49 21.96038235417727 27.293467971600283 28.96718475759975 21.778964916622694 50-51 21.865634628040155 27.5859308577259 28.944167248428077 21.60426726580587 52-53 22.008407389136437 27.719067922538347 28.55106790119133 21.721456787133885 54-55 21.493289038317855 27.449400949941293 28.881484149855908 22.17582586188494 56-57 21.874332906393427 27.763035009072475 28.48743195645213 21.87520012808197 58-59 22.05919240868433 27.307675373054675 28.625108073778367 22.008024144482633 60-61 21.919932642837914 27.364113964101755 29.07636424773105 21.639589145329282 62-63 21.939086778674803 27.357913124925577 28.923156157050666 21.779843939348957 64-65 21.616945567128738 27.490249407693 28.8262631740984 22.06654185107986 66-67 21.557959412400432 27.600935302672486 28.63984175906283 22.201263525864253 68-69 21.473242087236944 27.635939349687273 28.68865063161009 22.202167931465695 70-71 21.670709811182427 27.60641063827871 28.423367096063846 22.299512454475014 72-73 21.679641637314546 27.5227478919842 28.43723316255737 22.360377308143878 74-75 21.530879763048354 27.995116874799876 28.14674725157434 22.327256110577437 76-77 21.622833173768633 27.4528058427423 28.45014093396161 22.474220049527457 78-79 21.59322231765383 27.54627552679636 28.52030052617096 22.34020162937885 80-81 21.564355940259674 27.952134357357885 28.235225443841262 22.248284258541183 82-83 21.672576023908714 27.775735723315332 28.226225538429723 22.325462714346227 84-85 21.60809584800939 27.665372505069914 28.057723609776925 22.668808037143773 86-87 21.538710175057115 27.593917978999556 28.291662131018025 22.575709714925303 88-89 21.60985685152394 27.4280330673951 28.102998846867873 22.859111234213092 90-91 21.44816657636123 27.528982017720406 28.29993818151862 22.72291322439975 92-93 21.514769452449567 27.575381577542963 28.210321272280925 22.699527697726545 94-95 21.33785622798591 27.500400256163942 28.091111644786 23.070631871064148 96-97 21.152177777837075 27.38737037949967 28.233111936009692 23.22733990665356 98-99 21.230625239114794 27.472175960470135 27.848989188592405 23.44820961182267 100-101 21.30747390976019 26.9090370560123 28.524850769965603 23.258638264261908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1628.0 1 1066.0 2 702.0 3 864.5 4 669.5 5 584.0 6 1060.0 7 2243.5 8 3522.0 9 3965.0 10 3297.0 11 2546.0 12 2480.5 13 2650.0 14 2815.0 15 2947.0 16 3053.5 17 3165.5 18 3309.0 19 3465.5 20 3708.5 21 4214.5 22 4948.5 23 5793.5 24 6784.0 25 8136.0 26 9967.5 27 12129.5 28 13833.5 29 15833.5 30 18527.5 31 20967.5 32 23548.0 33 26194.5 34 28759.0 35 31217.5 36 34479.5 37 37970.0 38 40544.0 39 42140.5 40 43332.5 41 45864.5 42 48008.5 43 49506.0 44 51971.5 45 54270.5 46 54744.0 47 57793.5 48 69698.5 49 69575.0 50 58403.0 51 53782.0 52 50441.0 53 48409.5 54 45943.5 55 41733.5 56 37041.0 57 32747.5 58 29031.0 59 25053.5 60 20874.0 61 17007.5 62 13925.0 63 11118.0 64 8369.5 65 6284.5 66 4604.0 67 3629.0 68 2764.0 69 1852.5 70 1377.0 71 1108.0 72 925.0 73 807.0 74 657.0 75 537.0 76 375.0 77 214.5 78 138.0 79 86.5 80 47.0 81 30.5 82 16.5 83 6.5 84 4.0 85 4.0 86 4.5 87 2.5 88 0.5 89 1.0 90 1.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34815615327142707 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.005763688760808E-4 16-17 0.002067990180382111 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.6690148361618105E-4 26-27 0.0011674138115060305 28-29 0.0 30-31 0.0028685025082719607 32-33 0.0 34-35 0.011107108549471662 36-37 0.030452823140143023 38-39 0.02218086241861458 40-41 0.014342512541359804 42-43 0.010973689828156687 44-45 0.0028685025082719607 46-47 9.672857295335681E-4 48-49 7.338029672323621E-4 50-51 3.335468032874373E-5 52-53 1.0006404098623118E-4 54-55 0.0 56-57 0.0 58-59 0.004269399082079198 60-61 0.010973689828156687 62-63 0.004769719287010353 64-65 0.0019012167787383926 66-67 0.004002561639449247 68-69 0.0035689507951755786 70-71 3.335468032874373E-5 72-73 0.0 74-75 0.0 76-77 0.22040772761233854 78-79 1.6677340164371863E-4 80-81 0.0035022414345180918 82-83 3.3354680328743727E-4 84-85 0.0 86-87 0.1096034795602519 88-89 0.41022921336321916 90-91 0.3975210801579677 92-93 0.0 94-95 0.0 96-97 3.335468032874373E-5 98-99 0.0014342512541359804 100-101 8.005123278898495E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499040.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.261606445126525 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42429311717945 46.475495944475334 2 13.020696848247242 15.432548245242547 3 3.907568990387838 6.947064469966333 4 1.6214613853108049 3.843616259290343 5 0.8339479594916914 2.4710547885556466 6 0.4986535608982255 1.7730606647046943 7 0.3107631101964425 1.289142479388754 8 0.2301845038231808 1.091288278026885 9 0.16399097637251162 0.8746531832105844 >10 0.8813156634358518 9.632411929527397 >50 0.07055097020480094 2.885524891561906 >100 0.032509391745478344 3.4569850095644505 >500 0.002596139506879643 1.0639827531941763 >1k 0.0011287563073389753 1.2748929519129768 >5k 3.3862689220169265E-4 1.4882781513779224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9049 0.603653004589604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7899 0.5269372398334934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5301 0.353626320845341 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3787 0.25262834880990503 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2437 0.16257071192229694 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2185 0.1457599530366101 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1888 0.12594727292133634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1887 0.12588056356067884 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1753 0.11694150923257551 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.335468032874373E-5 0.0 20-21 0.0 0.0 0.0 6.670936065748746E-5 0.0 22-23 0.0 0.0 0.0 2.6683744262994986E-4 0.0 24-25 0.0 0.0 0.0 7.004482869036183E-4 0.0 26-27 0.0 0.0 0.0 0.0012674778524922616 0.0 28-29 0.0 0.0 0.0 0.002801793147614473 0.0 30-31 0.0 0.0 0.0 0.009039118369089551 0.0 32-33 0.0 0.0 0.0 0.020346355000533674 0.0 34-35 0.0 0.0 0.0 0.0361564734763582 0.0 36-37 0.0 0.0 0.0 0.06190628669014836 0.0 38-39 0.0 0.0 0.0 0.10353292774042053 0.0 40-41 0.0 0.0 0.0 0.1489620023481695 0.0 42-43 0.0 0.0 0.0 0.19322366314441242 0.0 44-45 0.0 0.0 0.0 0.2503602305475504 0.0 46-47 0.0 0.0 0.0 0.31860390650016013 0.0 48-49 0.0 0.0 0.0 0.4010566762728146 0.0 50-51 0.0 0.0 0.0 0.49098089443910775 0.0 52-53 0.0 0.0 0.0 0.5768691962856227 0.0 54-55 0.0 6.670936065748746E-5 0.0 0.6764662717472516 0.0 56-57 0.0 6.670936065748746E-5 0.0 0.7948086775536343 0.0 58-59 0.0 6.670936065748746E-5 0.0 0.9357655566229053 0.0 60-61 0.0 6.670936065748746E-5 0.0 1.09149855907781 0.0 62-63 0.0 6.670936065748746E-5 0.0 1.249599743836055 0.0 64-65 0.0 6.670936065748746E-5 0.0 1.4226104707012488 0.0 66-67 0.0 6.670936065748746E-5 0.0 1.6136994343046216 0.0 68-69 0.0 6.670936065748746E-5 0.0 1.8401443590564628 0.0 70-71 0.0 6.670936065748746E-5 0.0 2.0923057423417655 0.0 72-73 0.0 6.670936065748746E-5 0.0 2.356941776070018 0.0 74-75 0.0 6.670936065748746E-5 0.0 2.6225117408474756 0.0 76-77 0.0 6.670936065748746E-5 0.0 2.9158995090191056 0.0 78-79 0.0 6.670936065748746E-5 0.0 3.2582853025936602 0.0 80-81 0.0 6.670936065748746E-5 0.0 3.6301566335788236 0.0 82-83 0.0 6.670936065748746E-5 0.0 4.018972142170989 0.0 84-85 0.0 6.670936065748746E-5 0.0 4.4146587149108765 0.0 86-87 0.0 6.670936065748746E-5 0.0 4.823953997224891 0.0 88-89 0.0 6.670936065748746E-5 0.0 5.269572526416907 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1340 0.0 25.138529 2 GTACCTG 1455 0.0 23.885857 1 CATGGGG 3070 0.0 22.40866 4 GAGTACT 7605 0.0 22.399559 12-13 GTACATG 9010 0.0 22.350979 1 TACATGG 9075 0.0 22.062366 2 ACATGGG 9035 0.0 21.214828 3 GTATCAA 15520 0.0 21.043276 1 GGTATCA 12165 0.0 21.015665 1 AGTACTT 7825 0.0 20.860195 12-13 GTACTTT 8125 0.0 20.819986 14-15 GTATTAG 500 0.0 19.043243 1 TACTTTT 8815 0.0 18.409756 14-15 ACTTTTT 9290 0.0 18.335554 16-17 GTCTTAG 600 0.0 18.249777 1 ATCAACG 18050 0.0 18.031557 3 CAACGCA 18130 0.0 17.97816 5 TCAACGC 18140 0.0 17.942095 4 TATCAAC 18165 0.0 17.9174 2 AACGCAG 18540 0.0 17.580585 6 >>END_MODULE