FastQCFastQC Report
Fri 27 May 2016
SRR939212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473444
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.5998870673062566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.4819999945705436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48830.33140044684426423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33070.22444015517386479No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23440.15908307339810673No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21590.14652745540380224No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20310.13784032511585104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16920.11483300349385522No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16570.11245761630574355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90900.024.238931
TACATGG90950.023.5966322
TACCTGG12550.023.4046672
AGTACTT67850.022.19233912-13
GTATCAA138400.022.164391
ACATGGG93450.022.002143
GAGTACT66050.021.82932512-13
GGTATCA105600.021.1345631
GTACTTT70750.020.37952414-15
CATGGGG30450.019.7592744
CATGGGA62450.019.268854
GTATAGC7250.018.9941271
ATCAACG162600.018.8220333
TACTTTT79500.018.82145314-15
TATCAAC163250.018.718072
TCAACGC163600.018.6780224
CAACGCA163650.018.6716825
AACGCAG166900.018.4387366
GTACCTG16500.018.4185491
ACTTTTT83350.017.6973316-17
Produced by FastQC (version 0.11.5)