FastQCFastQC Report
Fri 27 May 2016
SRR939212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473444
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89220.60552012835235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76560.5195989803480824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.355833000779127No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36690.24900844551947682No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23190.15738636826374128No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20970.14231962667057588No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19380.13152858201601147No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.12833877636340438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15710.10662095064352634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG14250.026.974772
GTACATG84100.023.5762921
GAGTACT71250.023.17832212-13
TACATGG85450.022.8253442
GTACCTG17800.022.4924831
ACATGGG85800.021.7919713
GTATCAA147700.021.6531051
GTACTTT76650.021.45253814-15
AGTACTT73050.020.62581312-13
GGTATCA114900.020.160181
CATGGGA56050.019.3039784
CCTATAC3707.2759576E-1219.2387623
ACTTTTT89000.018.6623216-17
TATCAAC171050.018.5882842
ATCAACG170950.018.5436383
TCAACGC172300.018.4258884
GTATTAG5700.018.3961091
CAACGCA173200.018.3301415
CATGGGG30450.018.2341774
AACGCAG177100.017.9532816
Produced by FastQC (version 0.11.5)