##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1473444 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50794329475705 34.0 31.0 34.0 31.0 34.0 2 32.723083469748424 34.0 31.0 34.0 31.0 34.0 3 32.81926018226685 34.0 31.0 34.0 31.0 34.0 4 36.26067634738748 37.0 35.0 37.0 35.0 37.0 5 36.23200270929876 37.0 37.0 37.0 35.0 37.0 6 36.279585108086906 37.0 37.0 37.0 35.0 37.0 7 36.23969964247029 37.0 37.0 37.0 35.0 37.0 8 36.219929634244664 37.0 37.0 37.0 35.0 37.0 9 37.96289102266527 39.0 38.0 39.0 35.0 39.0 10-11 37.88405599398416 39.0 38.0 39.0 35.0 39.0 12-13 37.795129302504876 39.0 38.0 39.0 35.0 39.0 14-15 39.18665317446744 40.5 38.5 41.0 36.0 41.0 16-17 39.28903439832121 41.0 39.0 41.0 36.0 41.0 18-19 39.34947612532271 41.0 39.0 41.0 36.0 41.0 20-21 39.32951337139382 41.0 39.0 41.0 36.0 41.0 22-23 39.25389495630645 41.0 39.0 41.0 36.0 41.0 24-25 39.18110189460882 41.0 39.0 41.0 35.5 41.0 26-27 39.025164851870855 40.5 39.0 41.0 35.0 41.0 28-29 38.8720918473997 40.0 38.0 41.0 35.0 41.0 30-31 38.66050966307508 40.0 38.0 41.0 35.0 41.0 32-33 38.51288613615448 40.0 38.0 41.0 34.5 41.0 34-35 38.38785423809796 40.0 38.0 41.0 34.0 41.0 36-37 38.226023520405256 40.0 38.0 41.0 34.0 41.0 38-39 38.088519821588065 40.0 38.0 41.0 34.0 41.0 40-41 37.88899238790208 40.0 37.5 41.0 33.0 41.0 42-43 37.67900849981404 40.0 37.0 41.0 33.0 41.0 44-45 37.373220834996104 40.0 37.0 41.0 32.5 41.0 46-47 36.990266341985176 40.0 37.0 41.0 31.5 41.0 48-49 36.587621246548906 40.0 36.0 41.0 30.5 41.0 50-51 36.304580628785345 39.5 36.0 41.0 30.0 41.0 52-53 36.024208928198156 39.0 35.0 41.0 29.5 41.0 54-55 35.779266806203694 39.0 35.0 40.0 29.0 41.0 56-57 35.53962790577721 39.0 35.0 40.0 28.0 41.0 58-59 35.20929875855479 38.0 35.0 40.0 27.5 41.0 60-61 34.83232990191687 38.0 34.0 40.0 26.5 41.0 62-63 34.43275652145586 37.5 34.0 40.0 26.0 41.0 64-65 33.97843419906016 37.0 34.0 40.0 25.5 41.0 66-67 33.548521694750534 36.5 33.0 39.0 23.5 40.5 68-69 33.06174343918059 36.0 33.0 39.0 22.5 40.0 70-71 32.54426669761457 35.0 32.0 38.5 21.5 40.0 72-73 31.95987054818507 35.0 31.5 37.5 19.5 39.5 74-75 31.4116993927153 35.0 31.0 37.0 17.5 39.0 76-77 29.41732295221264 32.5 28.5 35.0 12.0 37.5 78-79 30.38067683603856 34.0 30.0 36.0 11.0 38.0 80-81 30.37942568567248 34.0 30.5 35.5 8.5 37.0 82-83 29.990296203995538 34.0 30.0 35.0 6.0 37.0 84-85 29.498998943970726 34.0 29.5 35.0 2.0 36.5 86-87 28.79153262696105 34.0 29.0 35.0 2.0 36.0 88-89 28.097935177719684 33.5 28.0 35.0 2.0 35.5 90-91 27.524715903692304 33.0 26.5 35.0 2.0 35.0 92-93 27.06723228029026 33.0 26.0 35.0 2.0 35.0 94-95 26.526165568559104 32.5 25.0 34.0 2.0 35.0 96-97 25.72342382879838 32.0 23.5 34.0 2.0 35.0 98-99 24.5179626779165 32.0 10.5 34.0 2.0 35.0 100-101 18.742030236642858 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1524945746337849 1101 2 0.09963125450299515 1101 3 0.19976936926663313 1101 4 0.1358303421360958 1101 5 0.08930386566375148 1101 6 0.13606353608037125 1101 7 0.1907019481819603 1101 8 0.13539368867818524 1101 9 0.17282373106449 1101 10-11 0.17790291315446893 1101 12-13 0.22656812219876343 1101 14-15 0.1918566493524878 1101 16-17 0.10528118290133648 1101 18-19 0.16447257208343302 1101 20-21 0.20239640180753327 1101 22-23 0.25539150408544486 1101 24-25 0.22065414165196984 1101 26-27 0.2834782696954363 1101 28-29 0.29327873964424356 1101 30-31 0.35971329778454475 1101 32-33 0.21566876173314142 1101 34-35 0.3072411101819128 1101 36-37 0.3991749948977059 1101 38-39 0.16488481174246772 1101 40-41 0.2415193153501889 1101 42-43 0.2836896718956652 1101 44-45 0.4243106507991854 1101 46-47 0.3805656665225996 1101 48-49 0.3410505837764788 1101 50-51 0.3949497571678009 1101 52-53 0.55565887513999 1101 54-55 0.4773586287198057 1101 56-57 0.41648174949727235 1101 58-59 0.3935882631172447 1101 60-61 0.3244303858757931 1101 62-63 0.2514893740367157 1101 64-65 0.2707947519695253 1101 66-67 0.2808867958949648 1101 68-69 0.23324114585621913 1101 70-71 0.25863457715118443 1101 72-73 0.6319426370755998 1101 74-75 0.2664877351020394 1101 76-77 0.3920611861198111 1101 78-79 0.40482546832932087 1101 80-81 0.5106024033232295 1101 82-83 0.5850379484141044 1101 84-85 0.4529747701778781 1101 86-87 0.5071383519253345 1101 88-89 0.35217072278901185 1101 90-91 0.4408239321789793 1101 92-93 0.3051029150755298 1101 94-95 0.06583826690902583 1101 96-97 0.056007853635012594 1101 98-99 0.07637362152564364 1101 100-101 -0.5297271454028554 1102 1 0.07116890279275623 1102 2 0.07435106438264683 1102 3 0.04623500255161872 1102 4 0.10199063437653422 1102 5 0.16277414630754805 1102 6 0.17847954119135068 1102 7 0.15433720447030908 1102 8 0.15916929793711176 1102 9 0.12505126893380947 1102 10-11 0.14810027985829777 1102 12-13 0.14616409158759325 1102 14-15 0.20091054703104305 1102 16-17 0.16977524971939317 1102 18-19 0.20135500099028292 1102 20-21 0.26476845650194036 1102 22-23 0.09479474294278134 1102 24-25 0.25483068659735864 1102 26-27 0.24486007278116517 1102 28-29 0.3126192223779256 1102 30-31 0.2576774215169024 1102 32-33 0.24183237262778334 1102 34-35 0.2509339892449347 1102 36-37 0.45325196778790655 1102 38-39 0.33293362951852146 1102 40-41 0.39233630089353255 1102 42-43 0.49178731241953955 1102 44-45 0.4917868453593286 1102 46-47 0.4953966948206556 1102 48-49 0.6044016414576703 1102 50-51 0.4003413272889773 1102 52-53 0.5107924105756396 1102 54-55 0.3680558941238914 1102 56-57 0.34889306871824033 1102 58-59 0.2503150011536235 1102 60-61 0.13725300691655207 1102 62-63 0.17629792994982552 1102 64-65 0.28390498970697564 1102 66-67 0.304718337527909 1102 68-69 0.30396856502461134 1102 70-71 0.33165398071319885 1102 72-73 0.4277077484493894 1102 74-75 0.2861252808888999 1102 76-77 0.2132528439926631 1102 78-79 0.42987291762662494 1102 80-81 0.3263661350317939 1102 82-83 0.48861706102835 1102 84-85 0.3590242293118244 1102 86-87 0.45429593082416275 1102 88-89 0.7412764014165063 1102 90-91 0.886927807298143 1102 92-93 0.6091938710592366 1102 94-95 0.6145103080553653 1102 96-97 0.3836755482144163 1102 98-99 0.6407005126924297 1102 100-101 0.16796272325550277 1103 1 0.014321340462700505 1103 2 0.045403865465239335 1103 3 0.02366693867960379 1103 4 0.08266721879126493 1103 5 0.08985624433685757 1103 6 0.07628395571894941 1103 7 0.110211793697232 1103 8 0.02169373062029223 1103 9 0.05354740756155962 1103 10-11 0.07912020103561446 1103 12-13 0.07653989891207402 1103 14-15 -0.09914447353544631 1103 16-17 0.04856355718683858 1103 18-19 0.06768301627082707 1103 20-21 0.1627284817126764 1103 22-23 0.16321739239375432 1103 24-25 0.12164479714535759 1103 26-27 0.10942580459984441 1103 28-29 0.18308010088902194 1103 30-31 0.15922492009774203 1103 32-33 0.256053142349856 1103 34-35 0.17966339340350146 1103 36-37 0.17400363059491042 1103 38-39 0.33239696830374044 1103 40-41 0.47514195248091085 1103 42-43 0.347909433998133 1103 44-45 0.2749230417285773 1103 46-47 0.31185253961209014 1103 48-49 0.2953714662286018 1103 50-51 0.28981740989687665 1103 52-53 0.31709172366132066 1103 54-55 0.2924504722503869 1103 56-57 0.19404659355605247 1103 58-59 0.263578629729885 1103 60-61 0.2904593996562497 1103 62-63 0.24096124200733016 1103 64-65 0.279239679090594 1103 66-67 0.20512328648941747 1103 68-69 0.01850151927386179 1103 70-71 0.03722893660344084 1103 72-73 -0.04845281528868739 1103 74-75 -0.0032395566348846216 1103 76-77 0.006845957873800046 1103 78-79 0.1862711653961533 1103 80-81 0.18715386821455482 1103 82-83 0.05595013470955479 1103 84-85 -0.033843383620297374 1103 86-87 -0.28714954493819533 1103 88-89 -0.19684442350672882 1103 90-91 0.05710840598692002 1103 92-93 0.1762557547491319 1103 94-95 -0.12523308180146486 1103 96-97 -0.33923449797516625 1103 98-99 -0.7545921587968643 1103 100-101 -1.0493420274754435 1104 1 0.11180598748784831 1104 2 0.04520374167852026 1104 3 0.1546540478073979 1104 4 0.1136098366308218 1104 5 0.05582069887192631 1104 6 0.048765484873499076 1104 7 0.13040805422475898 1104 8 0.0717995061927752 1104 9 0.18448463075991128 1104 10-11 0.16613721387776081 1104 12-13 0.14085823378935913 1104 14-15 0.10495713598185574 1104 16-17 0.13371390815756 1104 18-19 0.14984195168189274 1104 20-21 0.23242956638360113 1104 22-23 0.17672893837610815 1104 24-25 0.16125074470828338 1104 26-27 0.07931790597501731 1104 28-29 0.17028145964252417 1104 30-31 0.11446041938918938 1104 32-33 0.13817965190677484 1104 34-35 0.14173442516634083 1104 36-37 0.1394004876653412 1104 38-39 0.2744788241531353 1104 40-41 0.16221447792128885 1104 42-43 0.2358627361276291 1104 44-45 0.35424224960961936 1104 46-47 0.3193621121998831 1104 48-49 0.2939114326890149 1104 50-51 0.25570674550331063 1104 52-53 0.09010320380585313 1104 54-55 0.36280819496931116 1104 56-57 0.3084489514203099 1104 58-59 0.35161453367742723 1104 60-61 0.39943724276268 1104 62-63 0.26792284666366584 1104 64-65 0.12775380347941478 1104 66-67 0.24932715056069554 1104 68-69 0.1831897641186444 1104 70-71 0.10705096564981886 1104 72-73 0.006715221984222808 1104 74-75 -0.03982856560449122 1104 76-77 -0.04517404522376722 1104 78-79 0.14315319039364738 1104 80-81 0.32793254116761617 1104 82-83 0.2693896941355902 1104 84-85 0.3505804890011994 1104 86-87 0.29931060171483637 1104 88-89 0.17443392663797042 1104 90-91 0.22852603068579214 1104 92-93 0.28258268276779575 1104 94-95 0.42765702471659495 1104 96-97 -0.014700423855117606 1104 98-99 0.1325908117710206 1104 100-101 -0.5817388781651189 1105 1 0.11648484054745722 1105 2 0.13343686602875948 1105 3 0.14139323054880748 1105 4 0.09979459372770094 1105 5 0.10526956459764136 1105 6 0.03931981972161935 1105 7 0.13825116254943026 1105 8 0.09502182576893858 1105 9 0.2161896059497863 1105 10-11 0.05678909813047994 1105 12-13 0.130228810301233 1105 14-15 0.13766881648741247 1105 16-17 0.18133464469812566 1105 18-19 0.21184228762864876 1105 20-21 0.1888098308735664 1105 22-23 0.15983853419584193 1105 24-25 0.10960432186026736 1105 26-27 0.1463733326500929 1105 28-29 0.1990755583291559 1105 30-31 0.24135522719586788 1105 32-33 0.16406344156779085 1105 34-35 0.21597695602577716 1105 36-37 0.2807211443050619 1105 38-39 0.28259488921784737 1105 40-41 0.32695153148663536 1105 42-43 0.34011887510062877 1105 44-45 0.3248880505021532 1105 46-47 0.3081879101869305 1105 48-49 0.3545750750782304 1105 50-51 0.2347234089164374 1105 52-53 0.26871735533600116 1105 54-55 0.310492715995494 1105 56-57 0.4769810116364894 1105 58-59 0.2974771677723922 1105 60-61 0.21953945683339526 1105 62-63 0.15496500571175176 1105 64-65 0.2399441309367134 1105 66-67 0.14058623587755648 1105 68-69 0.23625289487003442 1105 70-71 0.23154420812619492 1105 72-73 -0.0998355127858126 1105 74-75 -0.06402836539838219 1105 76-77 -0.19754632870835565 1105 78-79 -0.051241662811243316 1105 80-81 -0.001598313726081102 1105 82-83 0.103216638427444 1105 84-85 0.04073231196634097 1105 86-87 0.06344221339670142 1105 88-89 0.12593303229322572 1105 90-91 0.07777928687243474 1105 92-93 -0.22371858883607487 1105 94-95 -0.14630971201464504 1105 96-97 -0.16703184895034084 1105 98-99 -0.49950010495504316 1105 100-101 -1.0370673228678307 1106 1 0.0025519528480941744 1106 2 0.07223647967637703 1106 3 0.07330847832684384 1106 4 0.10831942409622286 1106 5 0.14877034650127996 1106 6 0.15054561246678588 1106 7 0.1118170154814635 1106 8 0.1288243742929538 1106 9 0.16366517419880466 1106 10-11 0.17047148098730247 1106 12-13 0.19956800316641932 1106 14-15 0.23410359871520114 1106 16-17 0.132738074123111 1106 18-19 0.07780604409523306 1106 20-21 0.17310057637598675 1106 22-23 0.17463556778046296 1106 24-25 0.12581540737030394 1106 26-27 0.18289940267537474 1106 28-29 0.16083647322211903 1106 30-31 0.05555126817900202 1106 32-33 0.17531015078286316 1106 34-35 0.31818457443927883 1106 36-37 0.24115233021414895 1106 38-39 0.33132150329035426 1106 40-41 0.40619045670861453 1106 42-43 0.2532145016967391 1106 44-45 0.3679078083529461 1106 46-47 0.5031660957324391 1106 48-49 0.3962281442142839 1106 50-51 0.2521730560742057 1106 52-53 0.3705011688355526 1106 54-55 0.303430122090397 1106 56-57 0.2862072008089953 1106 58-59 0.3609427749632772 1106 60-61 0.3290174649722033 1106 62-63 0.20058946637345798 1106 64-65 0.21556056449242078 1106 66-67 0.38070813180771523 1106 68-69 0.26950296386153383 1106 70-71 0.19887947176133025 1106 72-73 0.12077410922838894 1106 74-75 0.17832261296675966 1106 76-77 0.11359656472934887 1106 78-79 0.1896632318617364 1106 80-81 0.5009324696230948 1106 82-83 0.36950821401803324 1106 84-85 0.4151510859764791 1106 86-87 0.23933521524961776 1106 88-89 0.04498492485046768 1106 90-91 0.37568868022909996 1106 92-93 0.5911980300110855 1106 94-95 0.48801509119532227 1106 96-97 0.09844086326969759 1106 98-99 0.0862795975273336 1106 100-101 -0.7978111059673232 1107 1 0.022304798349402688 1107 2 -0.040430449269315716 1107 3 0.015127929481899116 1107 4 -0.061515809716482295 1107 5 -0.11115564489777796 1107 6 0.014217741424580765 1107 7 0.01996526643866048 1107 8 2.662584442418847E-4 1107 9 0.04475572620570745 1107 10-11 0.14379963483169433 1107 12-13 0.1019148960469991 1107 14-15 0.02588240357856364 1107 16-17 0.022782811132728398 1107 18-19 0.13570639963288755 1107 20-21 -0.010601617271504438 1107 22-23 -0.049688915896837216 1107 24-25 -0.04009894635365896 1107 26-27 -0.025064818152436885 1107 28-29 0.07289621231362986 1107 30-31 0.01049597565614846 1107 32-33 0.14477534159085792 1107 34-35 -0.039053283068078315 1107 36-37 -0.008381463603228667 1107 38-39 0.029470609958963223 1107 40-41 0.06849803904214014 1107 42-43 -0.008902945737006007 1107 44-45 -0.0512720757503331 1107 46-47 -0.13673358775869104 1107 48-49 -0.19713623126770585 1107 50-51 -0.2310894375731749 1107 52-53 -0.1264292273901333 1107 54-55 -0.33093430869976714 1107 56-57 -0.28389043377510603 1107 58-59 -0.376974214769227 1107 60-61 -0.4619794596844926 1107 62-63 -0.3994082378154573 1107 64-65 -0.6292504328600899 1107 66-67 -0.6970126391256457 1107 68-69 -0.5407732952604007 1107 70-71 -0.540246732104606 1107 72-73 -0.8290738976423491 1107 74-75 -0.9220332899287804 1107 76-77 -0.5687226636276748 1107 78-79 -0.47055229262151954 1107 80-81 -0.39978909336509005 1107 82-83 -0.6724412113508862 1107 84-85 -0.6176977964331236 1107 86-87 -0.4174165042607072 1107 88-89 -0.40571070590797476 1107 90-91 -0.2557193720152142 1107 92-93 -0.14383484704718796 1107 94-95 -0.13198564972552518 1107 96-97 -0.42347474062775703 1107 98-99 -0.5442698735490978 1107 100-101 -1.3679040445742032 1108 1 0.024007741486997247 1108 2 0.020428649805289467 1108 3 0.0066522010450995595 1108 4 0.06524124072802095 1108 5 -0.006800269105660561 1108 6 -0.009188104863682156 1108 7 0.04811556129224215 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-0.11514989856539515 2316 98-99 -0.2860163581629145 2316 100-101 0.9037252982391628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 10.0 5 28.0 6 63.0 7 164.0 8 354.0 9 661.0 10 1185.0 11 1947.0 12 3038.0 13 4441.0 14 5562.0 15 7813.0 16 10629.0 17 15762.0 18 16545.0 19 10670.0 20 8905.0 21 9310.0 22 9795.0 23 10442.0 24 11863.0 25 13389.0 26 15575.0 27 18809.0 28 22397.0 29 27174.0 30 33432.0 31 40558.0 32 50803.0 33 66495.0 34 92773.0 35 138757.0 36 221278.0 37 351603.0 38 239435.0 39 11774.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74685628681297 19.502131250110754 15.010148485497002 21.740863977579274 2 15.290638802696268 21.071177459068686 40.593670339693944 23.044513398541106 3 19.629792513322528 25.680921704523552 30.104774935457336 24.584510846696585 4 12.658777666473922 17.30768186642994 37.336132218122984 32.697408248973154 5 13.174847500142523 38.37451576035465 33.72024997217404 14.73038676732879 6 30.5428642011505 36.6125214124188 17.74434589981024 15.100268486620461 7 27.758774680272886 33.94177179451679 20.52891049812548 17.77054302708484 8 26.007639245196966 35.250406530550194 19.550929658677223 19.191024565575617 9 26.65835959832881 17.29926620896349 20.71799131829917 35.32438287440853 10-11 24.816178965742843 27.33629510181588 26.78618936315191 21.061336569289367 12-13 26.30754884474741 24.682512535257533 27.661010530430747 21.348928089564314 14-15 22.936325256619998 25.772489901987434 27.004408410695156 24.286776430697415 16-17 21.315638284478858 29.251533775641686 27.559841768038893 21.872986171840562 18-19 21.148682949606503 27.494122613414557 30.51137335385668 20.845821083122264 20-21 22.38381641921919 26.26811741742475 30.319340266749194 21.02872589660686 22-23 21.80025165530553 26.449087987056174 30.236710726705596 21.5139496309327 24-25 21.74869395989324 26.535545160398183 30.536210269487952 21.179550610220623 26-27 21.663457511899438 26.48591105268195 30.649271848778564 21.201359586640052 28-29 21.108742510743536 27.02780017428555 30.745858003425987 21.117599311544925 30-31 21.438649857069557 26.655644870113825 30.54632547962461 21.359379793192005 32-33 21.279159574439205 26.751101500973228 30.4051596124454 21.564579312142165 34-35 21.98121179828476 26.65389458108351 30.173322518352098 21.19157110227963 36-37 21.868467009831623 26.42485586902028 30.414123053998416 21.292554067149677 38-39 21.642401728272286 26.707407099424874 30.512117761459734 21.13807341084311 40-41 21.64954646278986 26.781229722502015 30.33374555761592 21.235478257092204 42-43 21.465150895026095 26.939291078789314 29.81343357028295 21.78212445590165 44-45 21.5582219033796 27.010193198041655 29.621833268912496 21.809751629666255 46-47 21.878167713762036 27.192172457336504 29.08970405430032 21.83995577460114 48-49 21.953371508309743 27.2096816120093 29.009543291631346 21.827403588049606 50-51 21.788522594157595 27.58939321340488 28.93241231029011 21.68967188214742 52-53 22.048038473128262 27.718359163972302 28.570749889374824 21.662852473524612 54-55 21.509029186043037 27.465482230746467 28.870964895849454 22.15452368736104 56-57 21.848351106897248 27.783463629064713 28.45875273084877 21.90943253318927 58-59 22.05762383790615 27.30304638602216 28.638478693758323 22.000851082313368 60-61 21.912387912615955 27.287050937828322 29.132166059003506 21.668395090552217 62-63 21.89809358563967 27.345825580681144 28.95992691835087 21.79615391532831 64-65 21.623894353375945 27.443756882454046 28.848159277590472 22.084189486579533 66-67 21.516562073799477 27.577937324365937 28.699687765434163 22.205812836400423 68-69 21.43389933581019 27.59757536104207 28.724648324186525 22.243876978961215 70-71 21.625120508686635 27.583551841046965 28.47442535044656 22.316902299819844 72-73 21.619213217468733 27.45784705764183 28.495687654230494 22.427252070658945 74-75 21.45755115226639 27.99770469729423 28.109280026930104 22.435464123509274 76-77 21.638387298162616 27.41207943539539 28.42676043229833 22.522772834143662 78-79 21.634309600141734 27.52683449667628 28.444905874774385 22.393950028407605 80-81 21.53975939265853 27.884681947934016 28.208312803117607 22.36724585628985 82-83 21.67058944893732 27.76403446618942 28.17816625538534 22.38720982948792 84-85 21.537364161786943 27.594940832498555 28.125398725706575 22.742296280007928 86-87 21.55855000149473 27.56013577599678 28.256375030913773 22.624939191594713 88-89 21.538467829768557 27.405525801751057 28.05743627771046 22.998570090769928 90-91 21.402493074131417 27.457827092014455 28.280445450328852 22.859234383525276 92-93 21.54449032335128 27.472744128721555 28.206874506258806 22.775891041668363 94-95 21.28015088464539 27.422836369361974 28.10728745282734 23.189725293165296 96-97 21.10405960457269 27.2881086759999 28.229678223264678 23.37815349616273 98-99 21.120357041781787 27.38002403896729 27.85364327448469 23.64597564476623 100-101 21.465663889608106 26.44377126611406 28.637071780589864 23.453493063687972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1438.0 1 989.5 2 680.5 3 807.5 4 639.0 5 552.5 6 1006.0 7 2154.5 8 3406.0 9 3829.5 10 3201.5 11 2494.5 12 2385.5 13 2575.0 14 2815.0 15 2945.5 16 2993.5 17 3118.0 18 3274.5 19 3420.5 20 3682.0 21 4124.5 22 4755.0 23 5743.0 24 6834.0 25 7988.0 26 9737.5 27 11890.5 28 13761.5 29 15604.5 30 17922.0 31 20472.5 32 23000.5 33 25549.0 34 28379.5 35 31035.0 36 34182.0 37 37316.5 38 39514.0 39 40956.5 40 42234.5 41 44823.5 42 47104.5 43 48662.5 44 51082.0 45 53387.5 46 54103.5 47 57224.0 48 68256.0 49 67993.0 50 57672.5 51 53127.0 52 49485.0 53 47631.0 54 45583.0 55 41314.0 56 36292.5 57 32027.5 58 28427.0 59 24622.5 60 20604.0 61 16834.0 62 13797.5 63 10951.0 64 8188.5 65 6147.5 66 4572.5 67 3663.0 68 2811.0 69 1897.0 70 1410.5 71 1103.0 72 931.5 73 844.0 74 685.5 75 561.0 76 397.0 77 220.0 78 118.0 79 77.5 80 53.5 81 30.0 82 13.5 83 7.0 84 5.0 85 3.0 86 0.5 87 1.5 88 2.0 89 1.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4228189194838759 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.126161564334987E-4 16-17 0.0025450577015482096 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.018023080619284E-4 26-27 0.0037327512956040407 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012148408762056787 36-37 0.026570402404163307 38-39 0.02012292289357451 40-41 0.009230075930948172 42-43 0.009162207725573555 44-45 0.004038158219789825 46-47 0.005599126943406061 48-49 0.0035970148848548027 50-51 6.786820537461893E-4 52-53 0.0 54-55 0.0 56-57 6.786820537461892E-5 58-59 0.001764573339740092 60-61 0.002714728214984757 62-63 0.0015949028263035445 64-65 0.002816530523046685 66-67 0.0022057166746751147 68-69 0.004750774376223324 70-71 1.696705134365473E-4 72-73 0.0 74-75 0.0 76-77 0.09535482855133959 78-79 0.016763446727530874 80-81 4.072092322477136E-4 82-83 0.0 84-85 0.0 86-87 0.10913207424238722 88-89 0.4220045010193805 90-91 0.4275357597574119 92-93 0.0 94-95 3.393410268730946E-5 96-97 0.0 98-99 2.036046161238568E-4 100-101 1.696705134365473E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1473444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.67581435084091 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73669339469349 46.986762976208105 2 12.89718527508304 15.39300068248509 3 3.78245481512715 6.771632140139163 4 1.6158121495742277 3.856996234552992 5 0.7949698585016176 2.3720236845228397 6 0.48084715018296637 1.7216967159269774 7 0.32646385095564096 1.3637397313322597 8 0.21575986869093963 1.030051669468986 9 0.165243549959589 0.8874939069056105 >10 0.8785899817190054 9.571159424869307 >50 0.06981382023065408 2.8592124983639033 >100 0.03218583448632023 3.4448246098452144 >500 0.002501997642845976 1.0161817574754868 >1k 0.0011372716558390801 1.2442848020807837 >5k 3.4118149675172404E-4 1.4809391658233217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8922 0.60552012835235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7656 0.5195989803480824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5243 0.355833000779127 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3669 0.24900844551947682 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2319 0.15738636826374128 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2097 0.14231962667057588 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1938 0.13152858201601147 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.12833877636340438 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1571 0.10662095064352634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.393410268730946E-5 0.0 12-13 0.0 0.0 0.0 6.786820537461892E-5 0.0 14-15 0.0 0.0 0.0 6.786820537461892E-5 0.0 16-17 0.0 0.0 0.0 6.786820537461892E-5 0.0 18-19 0.0 0.0 0.0 1.3573641074923783E-4 0.0 20-21 0.0 1.3573641074923783E-4 0.0 1.3573641074923783E-4 0.0 22-23 0.0 1.3573641074923783E-4 0.0 3.054069241857851E-4 0.0 24-25 0.0 1.3573641074923783E-4 0.0 5.768797456842608E-4 0.0 26-27 0.0 1.3573641074923783E-4 0.0 0.0010858912859939027 0.0 28-29 0.0 1.3573641074923783E-4 0.0 0.003189805652607089 0.0 30-31 0.0 1.3573641074923783E-4 0.0 0.009230075930948173 0.0 32-33 0.0 1.3573641074923783E-4 0.0 0.02005505468819989 0.0 34-35 0.0 1.3573641074923783E-4 0.0 0.036852435518418075 0.0 36-37 0.0 1.696705134365473E-4 0.0 0.06199760560971438 0.0 38-39 0.0 2.3753871881116622E-4 0.0 0.1044491680715385 0.0 40-41 0.0 2.7147282149847567E-4 0.0 0.15005660208328242 0.0 42-43 0.0 2.7147282149847567E-4 0.0 0.19824302789926185 0.0 44-45 0.0 2.7147282149847567E-4 0.0 0.2569150914456199 0.0 46-47 0.0 3.054069241857851E-4 0.0 0.32827850939703174 0.0 48-49 6.786820537461892E-5 3.393410268730946E-4 0.0 0.41413178919592464 0.0 50-51 6.786820537461892E-5 3.393410268730946E-4 0.0 0.5053127231167251 0.0 52-53 6.786820537461892E-5 3.393410268730946E-4 0.0 0.592693037536547 0.0 54-55 6.786820537461892E-5 3.393410268730946E-4 0.0 0.6898803076330013 0.0 56-57 6.786820537461892E-5 3.393410268730946E-4 0.0 0.8080727872929001 0.0 58-59 6.786820537461892E-5 4.0720923224771353E-4 0.0 0.947541949337742 0.0 60-61 6.786820537461892E-5 4.0720923224771353E-4 0.0 1.1122241496792549 0.0 62-63 6.786820537461892E-5 4.0720923224771353E-4 0.0 1.2764312725831453 0.0 64-65 6.786820537461892E-5 4.0720923224771353E-4 0.0 1.4407401977950978 0.0 66-67 6.786820537461892E-5 4.0720923224771353E-4 0.0 1.6339609784966378 0.0 68-69 6.786820537461892E-5 4.0720923224771353E-4 0.0 1.8588762111081247 0.0 70-71 6.786820537461892E-5 4.0720923224771353E-4 0.0 2.1113120009990203 0.0 72-73 6.786820537461892E-5 4.0720923224771353E-4 0.0 2.3756247268304733 0.0 74-75 6.786820537461892E-5 4.7507743762233243E-4 0.0 2.6483531101283795 0.0 76-77 6.786820537461892E-5 4.7507743762233243E-4 0.0 2.938761160926374 6.786820537461892E-5 78-79 6.786820537461892E-5 4.7507743762233243E-4 0.0 3.274776645736112 6.786820537461892E-5 80-81 6.786820537461892E-5 4.7507743762233243E-4 0.0 3.656128091736096 6.786820537461892E-5 82-83 6.786820537461892E-5 4.7507743762233243E-4 0.0 4.048304516493332 6.786820537461892E-5 84-85 6.786820537461892E-5 4.7507743762233243E-4 0.0 4.44825185076596 6.786820537461892E-5 86-87 6.786820537461892E-5 4.7507743762233243E-4 0.0 4.860381527903334 6.786820537461892E-5 88-89 6.786820537461892E-5 4.7507743762233243E-4 0.0 5.315302108529405 6.786820537461892E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1425 0.0 26.97477 2 GTACATG 8410 0.0 23.576292 1 GAGTACT 7125 0.0 23.178322 12-13 TACATGG 8545 0.0 22.825344 2 GTACCTG 1780 0.0 22.492483 1 ACATGGG 8580 0.0 21.791971 3 GTATCAA 14770 0.0 21.653105 1 GTACTTT 7665 0.0 21.452538 14-15 AGTACTT 7305 0.0 20.625813 12-13 GGTATCA 11490 0.0 20.16018 1 CATGGGA 5605 0.0 19.303978 4 CCTATAC 370 7.2759576E-12 19.238762 3 ACTTTTT 8900 0.0 18.66232 16-17 TATCAAC 17105 0.0 18.588284 2 ATCAACG 17095 0.0 18.543638 3 TCAACGC 17230 0.0 18.425888 4 GTATTAG 570 0.0 18.396109 1 CAACGCA 17320 0.0 18.330141 5 CATGGGG 3045 0.0 18.234177 4 AACGCAG 17710 0.0 17.953281 6 >>END_MODULE