FastQCFastQC Report
Fri 27 May 2016
SRR939211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178977
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82950.38068322887299866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.31166001293267437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.19527512222478713No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25710.11799114905756233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121600.030.2152081
GGTATCA84500.028.931351
TATCAAC147450.024.7098872
TCAACGC148050.024.4817354
CAACGCA149350.024.2686375
ATCAACG149100.024.2457773
AACGCAG152150.023.9372356
GTACATG102800.023.7349431
TACATGG102700.022.7900712
GAGTACT78850.022.37402512-13
ACATGGG102650.021.462643
ACGCAGA169300.021.4569477
AGTACTT81300.021.37938112-13
CGCAGAG171550.021.1207948
GTACTTT85250.020.66869514-15
GCAGAGT180350.019.7737249
AGAGTAC144250.019.76492910-11
GTAAGGT9600.018.7543324
CATGGGG53500.018.6860664
TACTTTT95250.018.2998414-15
Produced by FastQC (version 0.11.5)