##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2178977 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11848449983639 33.0 31.0 34.0 30.0 34.0 2 32.214572710037785 34.0 31.0 34.0 30.0 34.0 3 32.27254670425617 34.0 31.0 34.0 30.0 34.0 4 35.71394007371349 37.0 35.0 37.0 35.0 37.0 5 35.75826270768347 37.0 35.0 37.0 35.0 37.0 6 35.846374238920376 37.0 37.0 37.0 35.0 37.0 7 35.7703367222325 37.0 36.0 37.0 35.0 37.0 8 35.758013967104745 37.0 36.0 37.0 35.0 37.0 9 37.444469583662425 39.0 38.0 39.0 35.0 39.0 10-11 37.35957676469279 39.0 37.5 39.0 34.5 39.0 12-13 37.26944639617582 39.0 37.0 39.0 34.0 39.0 14-15 38.58508327531681 40.0 38.0 41.0 34.0 41.0 16-17 38.522864169745716 40.0 38.0 41.0 34.0 41.0 18-19 38.45706655003701 40.0 38.0 41.0 34.0 41.0 20-21 38.363672723484456 40.0 38.0 41.0 34.0 41.0 22-23 38.28283800150254 40.0 38.0 41.0 34.0 41.0 24-25 38.31722982849291 40.0 38.0 41.0 34.0 41.0 26-27 38.240080551561576 40.0 38.0 41.0 34.0 41.0 28-29 38.114950043070664 40.0 38.0 41.0 33.5 41.0 30-31 37.94272105671607 40.0 38.0 41.0 33.0 41.0 32-33 37.737013286510134 40.0 38.0 41.0 33.0 41.0 34-35 37.42269881692188 40.0 37.0 41.0 32.5 41.0 36-37 37.175571151049326 40.0 37.0 41.0 31.0 41.0 38-39 36.93623223191433 40.0 36.5 41.0 30.5 41.0 40-41 36.774701614565004 40.0 36.0 41.0 30.5 41.0 42-43 36.570694871951375 40.0 36.0 41.0 30.0 41.0 44-45 36.29046956438732 39.5 36.0 41.0 30.0 41.0 46-47 35.892664309903225 39.0 35.0 41.0 28.5 41.0 48-49 35.84247217845805 39.0 35.5 41.0 28.0 41.0 50-51 34.827070226073985 38.0 34.0 40.0 25.5 40.5 52-53 34.98459644135757 38.5 35.0 40.0 26.5 40.5 54-55 35.476404753239706 39.0 35.0 41.0 27.0 41.0 56-57 35.358298871442884 39.0 35.0 41.0 26.0 41.0 58-59 35.16328258627787 39.0 35.0 41.0 26.0 41.0 60-61 34.87309480549818 38.5 35.0 40.5 26.0 41.0 62-63 34.507437664555425 38.0 34.5 40.0 25.5 41.0 64-65 34.131825163826875 37.0 34.0 40.0 23.5 41.0 66-67 33.74220792601299 37.0 34.0 39.5 23.0 41.0 68-69 33.34405755544918 36.0 34.0 39.0 22.0 41.0 70-71 32.936370140666924 36.0 33.0 39.0 21.5 40.5 72-73 32.492266095511795 35.0 33.0 38.5 20.0 40.0 74-75 32.04317668337022 35.0 32.5 37.0 18.5 39.5 76-77 31.614073943873663 35.0 32.0 37.0 17.5 39.0 78-79 31.200850903887464 35.0 32.0 36.0 13.0 39.0 80-81 30.789462669867557 35.0 31.0 36.0 7.5 37.5 82-83 30.3797247515692 35.0 31.0 35.0 6.0 37.0 84-85 30.02238550475751 34.0 31.0 35.0 2.0 37.0 86-87 29.652942871815537 34.0 30.0 35.0 2.0 36.0 88-89 29.27958991765402 34.0 30.0 35.0 2.0 36.0 90-91 28.92509374812125 34.0 29.5 35.0 2.0 35.5 92-93 28.535115102178686 34.0 29.0 35.0 2.0 35.0 94-95 28.09538099759658 34.0 29.0 35.0 2.0 35.0 96-97 27.563621828041324 34.0 28.0 35.0 2.0 35.0 98-99 26.898925275484782 34.0 26.5 35.0 2.0 35.0 100-101 24.40725762594098 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.162759222430509 1101 2 0.2276466435530935 1101 3 0.21674493602203881 1101 4 0.09403965410636772 1101 5 0.2738420898826206 1101 6 0.32260114364811443 1101 7 0.2361219349286685 1101 8 0.32469670819077834 1101 9 0.4278763515334134 1101 10-11 0.4083522306563694 1101 12-13 0.23987892568306535 1101 14-15 0.3131217678231195 1101 16-17 0.3766098533471691 1101 18-19 0.4344682426768074 1101 20-21 0.4476374598527926 1101 22-23 0.358075700998711 1101 24-25 0.2268007259831748 1101 26-27 0.24822906926293342 1101 28-29 0.2490819439318841 1101 30-31 0.23596710431429102 1101 32-33 0.2842278005408403 1101 34-35 0.34328422181820883 1101 36-37 0.27810269064244864 1101 38-39 0.3321060830822873 1101 40-41 0.3007338341363166 1101 42-43 0.19360546943237011 1101 44-45 0.21344618793917647 1101 46-47 0.1342436425895599 1101 48-49 0.3509858224421407 1101 50-51 0.3387156342268298 1101 52-53 0.3893818503816817 1101 54-55 0.4362921172445553 1101 56-57 0.30360969958096007 1101 58-59 0.38776101935446405 1101 60-61 0.30421494386701653 1101 62-63 0.3390004998287779 1101 64-65 0.2984147001842814 1101 66-67 0.33515258225849465 1101 68-69 0.2617268709179754 1101 70-71 0.16824177307063337 1101 72-73 0.179077429774253 1101 74-75 0.024913407806174348 1101 76-77 -0.17562860781747247 1101 78-79 -0.08884754433513109 1101 80-81 -0.08491376095100733 1101 82-83 -0.10340283478429413 1101 84-85 -0.22231560281856844 1101 86-87 -0.1578220177200116 1101 88-89 0.02011009544358089 1101 90-91 -0.3654533586796269 1101 92-93 -0.4368634875859776 1101 94-95 -0.3383148518021031 1101 96-97 -0.2589740743074884 1101 98-99 -0.41482012247977096 1101 100-101 -0.5075394427118916 1102 1 0.20127697511631482 1102 2 0.28313794652187596 1102 3 0.28159967974170286 1102 4 0.18267101690833698 1102 5 0.19484520752988033 1102 6 0.194038532648932 1102 7 0.3210093028762486 1102 8 0.3004371355991111 1102 9 0.3541748278537682 1102 10-11 0.21557821532342558 1102 12-13 0.32695616177439746 1102 14-15 0.43249721057655677 1102 16-17 0.4375809380253841 1102 18-19 0.45192695678269956 1102 20-21 0.5258085138076538 1102 22-23 0.5795362706628211 1102 24-25 0.4126559073449272 1102 26-27 0.36858437163935065 1102 28-29 0.4686457882860253 1102 30-31 0.4910314852220452 1102 32-33 0.5030877981758053 1102 34-35 0.5822318252304868 1102 36-37 0.6121446136594386 1102 38-39 0.6271350050350222 1102 40-41 0.5652644353747505 1102 42-43 0.5198476103862788 1102 44-45 0.4229198128202327 1102 46-47 0.4185243623560595 1102 48-49 0.24581040090772177 1102 50-51 0.36513357799424284 1102 52-53 0.28757425596536734 1102 54-55 0.44942939208267774 1102 56-57 0.3531035930372113 1102 58-59 0.3729305061970294 1102 60-61 0.24729080514331514 1102 62-63 0.11914061160433675 1102 64-65 0.22232341312975734 1102 66-67 0.2670899828634532 1102 68-69 0.17892921628167358 1102 70-71 0.03897255246034348 1102 72-73 -0.009672986040982323 1102 74-75 -0.17316908993654323 1102 76-77 -0.12138350725373215 1102 78-79 -0.11214314393241054 1102 80-81 -0.13810592340021444 1102 82-83 -0.11455718208422994 1102 84-85 -0.16010024975966175 1102 86-87 -0.44290950193008527 1102 88-89 -0.46042892266883584 1102 90-91 -0.5846864848697386 1102 92-93 -0.6622818999398632 1102 94-95 -0.6900804502610711 1102 96-97 -0.8245452359046155 1102 98-99 -0.7471751618288778 1102 100-101 -0.7810088282115721 1103 1 0.2415220551755013 1103 2 0.3331583283097572 1103 3 0.41657830207075364 1103 4 0.3651515375151604 1103 5 0.38940919772434057 1103 6 0.27347737972664987 1103 7 0.20958895084550733 1103 8 0.30234217635870664 1103 9 0.4154753423977766 1103 10-11 0.31621001096556967 1103 12-13 0.29824237326283054 1103 14-15 0.3271429931377554 1103 16-17 0.3021704127481186 1103 18-19 0.38944596939712994 1103 20-21 0.38748566045282473 1103 22-23 0.40020252836765735 1103 24-25 0.44071766429483006 1103 26-27 0.38883779536863017 1103 28-29 0.4472657304001544 1103 30-31 0.4880415816993846 1103 32-33 0.39535473302807844 1103 34-35 0.3704020605002398 1103 36-37 0.22715571424151193 1103 38-39 0.31137297414434073 1103 40-41 0.2968130826048636 1103 42-43 0.2922500065525355 1103 44-45 0.22312511590418893 1103 46-47 0.21445413125018575 1103 48-49 0.26185180948684916 1103 50-51 0.2555566041898132 1103 52-53 0.3479990299290847 1103 54-55 0.2313900113389522 1103 56-57 0.25268953907175273 1103 58-59 0.32832077964184947 1103 60-61 0.2906908851852634 1103 62-63 0.15403842286288238 1103 64-65 0.22860609592999737 1103 66-67 0.11855727267974459 1103 68-69 0.04305708395414598 1103 70-71 0.05098030310926305 1103 72-73 -0.0408443911749643 1103 74-75 0.041206475469103054 1103 76-77 0.0799680028683376 1103 78-79 -0.06462957618597542 1103 80-81 -0.08162251247817309 1103 82-83 0.04501776282394587 1103 84-85 -0.10074522129279018 1103 86-87 -0.359506350696158 1103 88-89 -0.09586729554177609 1103 90-91 -0.26258183254432055 1103 92-93 -0.2445102804589574 1103 94-95 -0.2147319376011545 1103 96-97 -0.33594909155010555 1103 98-99 -0.6164175719666787 1103 100-101 -0.7333882461331704 1104 1 0.21547195013580023 1104 2 0.21356203370032745 1104 3 0.2511190404758423 1104 4 0.11176619687900313 1104 5 0.22659753345172362 1104 6 0.16872859913440408 1104 7 0.2147248017579102 1104 8 0.248366433457484 1104 9 0.387992441355685 1104 10-11 0.3501271288682517 1104 12-13 0.34946268932916524 1104 14-15 0.3875952542486658 1104 16-17 0.371450290671099 1104 18-19 0.4680721098142513 1104 20-21 0.41675443102769805 1104 22-23 0.3066497211022039 1104 24-25 0.39719606400624485 1104 26-27 0.40511862442316726 1104 28-29 0.38032332641189015 1104 30-31 0.26144765778212076 1104 32-33 0.3573927756953523 1104 34-35 0.2874378259016268 1104 36-37 0.33875056593375774 1104 38-39 0.4529096549936398 1104 40-41 0.2591180389994392 1104 42-43 0.24081961108682037 1104 44-45 0.22580891591482555 1104 46-47 0.4582779396432457 1104 48-49 0.4010616566180758 1104 50-51 0.3889010967747524 1104 52-53 0.4700602215554994 1104 54-55 0.38764496650081526 1104 56-57 0.26220072492390045 1104 58-59 0.37517159991171667 1104 60-61 0.28954096323514733 1104 62-63 0.334000040227707 1104 64-65 0.2599554181352204 1104 66-67 0.2166813505817018 1104 68-69 0.21611268168128817 1104 70-71 0.1946478819798685 1104 72-73 0.22575843419183173 1104 74-75 0.23141189652972116 1104 76-77 0.2204392347069799 1104 78-79 0.12793330165525418 1104 80-81 0.20650832585411294 1104 82-83 0.10093742140275097 1104 84-85 0.12630532362904034 1104 86-87 0.19863983809502272 1104 88-89 0.29616460953711155 1104 90-91 0.1950689373791583 1104 92-93 0.07762435461404849 1104 94-95 -0.09984056512999118 1104 96-97 -0.17243335635654944 1104 98-99 -0.1538920257148213 1104 100-101 -0.446895352370543 1105 1 0.010721859524188915 1105 2 0.10763297110939618 1105 3 0.20112184815970835 1105 4 0.06806306605624712 1105 5 0.020359625635641976 1105 6 0.18287319995832974 1105 7 0.1411839040137508 1105 8 0.1136958768724412 1105 9 0.2136620739574866 1105 10-11 0.13715677800986015 1105 12-13 0.264622778358266 1105 14-15 0.3155075881486411 1105 16-17 0.22070548871490558 1105 18-19 0.24906842919595107 1105 20-21 0.24615037060634393 1105 22-23 0.3268822738994217 1105 24-25 0.2383036187177865 1105 26-27 0.24645513299171284 1105 28-29 0.2779259472571596 1105 30-31 0.1834073190223009 1105 32-33 0.17417570599842946 1105 34-35 0.10945325795312755 1105 36-37 0.07157494275556076 1105 38-39 0.2518907209946306 1105 40-41 0.2842256243402588 1105 42-43 0.23645634273861305 1105 44-45 0.2418175635811295 1105 46-47 0.11665619591841647 1105 48-49 0.2501526051557832 1105 50-51 0.2508248196614531 1105 52-53 0.19325296650038837 1105 54-55 0.3348459609313892 1105 56-57 0.17693123832165725 1105 58-59 0.2838135232262218 1105 60-61 0.20702032675873738 1105 62-63 0.3966399777439875 1105 64-65 0.39380689676363545 1105 66-67 0.25926102499247605 1105 68-69 0.38460268377428974 1105 70-71 0.5111938182403364 1105 72-73 0.34994024958916725 1105 74-75 0.35219067146068284 1105 76-77 0.4954183046999816 1105 78-79 0.3956781189260816 1105 80-81 0.17222101811664814 1105 82-83 0.21763637643386247 1105 84-85 0.34351731907548455 1105 86-87 0.19880510857707634 1105 88-89 0.28111504115469543 1105 90-91 0.1448115238175376 1105 92-93 0.14678475504081945 1105 94-95 -0.04163179086266666 1105 96-97 0.1567774037090004 1105 98-99 0.04538895197175208 1105 100-101 0.09128774435161446 1106 1 0.2017230392001892 1106 2 0.18119082434701994 1106 3 0.27998275987664556 1106 4 0.24167260760383158 1106 5 0.2367040509751206 1106 6 0.1966030264973213 1106 7 0.1836407962493496 1106 8 0.24727034284732952 1106 9 0.2612393990016173 1106 10-11 0.25351376780475476 1106 12-13 0.26576633453306187 1106 14-15 0.29045914227879877 1106 16-17 0.346456614270771 1106 18-19 0.3457849340743948 1106 20-21 0.36813859673411287 1106 22-23 0.3839662664494341 1106 24-25 0.35998238254703807 1106 26-27 0.2483355098395421 1106 28-29 0.2048335987482517 1106 30-31 0.23827798857664817 1106 32-33 0.3316992875977647 1106 34-35 0.26913592320074287 1106 36-37 0.30663230056740787 1106 38-39 0.37689155544612873 1106 40-41 0.356405917361478 1106 42-43 0.2350552986728971 1106 44-45 0.23641007945716552 1106 46-47 0.381874803137805 1106 48-49 0.36293456631707244 1106 50-51 0.2558553701228803 1106 52-53 0.2326498058842077 1106 54-55 0.2246900186353784 1106 56-57 0.2988101513031438 1106 58-59 0.28259129019586027 1106 60-61 0.40250012683369363 1106 62-63 0.2156531697069326 1106 64-65 0.17956981907954628 1106 66-67 0.17255948778976204 1106 68-69 0.14902069438469567 1106 70-71 0.1429489415341223 1106 72-73 0.1517189187200927 1106 74-75 0.1452170172402134 1106 76-77 0.23940441275144053 1106 78-79 0.2574209495572255 1106 80-81 0.3490366415282118 1106 82-83 0.3500703856252265 1106 84-85 0.28960877209872393 1106 86-87 0.20014344957305852 1106 88-89 0.5798216174907935 1106 90-91 0.560809079589891 1106 92-93 0.5453149760131133 1106 94-95 0.3788615511680362 1106 96-97 0.48081697387587496 1106 98-99 0.5802807069233822 1106 100-101 0.48936557340444864 1107 1 0.17752986201631415 1107 2 0.15141246414644627 1107 3 0.154224313748756 1107 4 0.2231921143181097 1107 5 0.17758852044717344 1107 6 0.13882257288877042 1107 7 0.1266090075638786 1107 8 0.11992619081104294 1107 9 0.2484941439216044 1107 10-11 0.21856621347419747 1107 12-13 0.2740446859880947 1107 14-15 0.36464486273241903 1107 16-17 0.417057005334847 1107 18-19 0.3175384256192899 1107 20-21 0.3135560217318343 1107 22-23 0.2907839503153724 1107 24-25 0.30499682190679067 1107 26-27 0.30908565229991325 1107 28-29 0.3084501589493769 1107 30-31 0.40409311506028445 1107 32-33 0.3333256142743579 1107 34-35 0.27343021732917094 1107 36-37 0.3167320274301417 1107 38-39 0.4443829042581555 1107 40-41 0.32096861235267227 1107 42-43 0.31038507486972833 1107 44-45 0.37129415952219347 1107 46-47 0.4414401540137831 1107 48-49 0.49805420080840435 1107 50-51 0.3475215995121559 1107 52-53 0.4253379295223283 1107 54-55 0.45377573144816097 1107 56-57 0.3835930591885983 1107 58-59 0.4761470642366774 1107 60-61 0.5033308848333391 1107 62-63 0.36283475581789304 1107 64-65 0.3704735057422184 1107 66-67 0.2566708296605995 1107 68-69 0.37036958926965724 1107 70-71 0.18939666110951237 1107 72-73 0.1733627687127708 1107 74-75 0.3666545183334051 1107 76-77 0.4766523683645687 1107 78-79 0.3284988935617825 1107 80-81 0.30728470577024325 1107 82-83 0.2801933434247381 1107 84-85 0.3163188258884482 1107 86-87 0.30129088846239327 1107 88-89 0.2925343989708544 1107 90-91 0.34061356136094645 1107 92-93 0.3218075418371704 1107 94-95 0.26570440380002935 1107 96-97 0.38895178327356916 1107 98-99 0.2786651740939803 1107 100-101 0.09054212576220166 1108 1 -0.11741998762263961 1108 2 -0.040702134380559585 1108 3 0.031178557171585908 1108 4 -0.046172947897147765 1108 5 0.0045715755675530545 1108 6 0.020483027033151302 1108 7 0.09864095253353611 1108 8 0.15906868266273477 1108 9 0.09363577142237034 1108 10-11 0.17324721396039422 1108 12-13 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-1.1145728054978647 2316 98-99 -0.9953282909853911 2316 100-101 -0.9226745954801139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7226.0 3 1875.0 4 1835.0 5 1930.0 6 2362.0 7 2686.0 8 3337.0 9 4178.0 10 4995.0 11 6109.0 12 8268.0 13 12549.0 14 11072.0 15 11754.0 16 14680.0 17 20368.0 18 18908.0 19 11824.0 20 11690.0 21 12106.0 22 12742.0 23 13991.0 24 15780.0 25 17782.0 26 20211.0 27 24013.0 28 27930.0 29 33411.0 30 40179.0 31 49666.0 32 62679.0 33 82533.0 34 115625.0 35 173882.0 36 284908.0 37 475076.0 38 472732.0 39 86055.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9484237072659 18.654297663706103 14.443314287450853 22.953964341577134 2 15.408118444678461 19.873639773652453 40.68710153477456 24.031140246894523 3 19.50596933051429 24.306104953262526 29.496385690513666 26.691540025709514 4 12.994435944442149 16.307001704595056 36.538615280706836 34.15994707025597 5 14.159176133230398 37.00369471710599 33.63690673137017 15.200222418293446 6 32.365977719372864 35.138470781365264 17.4393630536971 15.056188445564775 7 29.080807454414746 32.62817730188588 20.232341815325462 18.058673428373908 8 26.47348503293297 34.760704248256424 19.55250853758144 19.21330218122917 9 26.783513611958696 17.177079711339847 20.494576942739698 35.544829733961755 10-11 25.554596084285468 26.312269621634037 26.601779870241714 21.531354423838778 12-13 26.363152178381615 23.765313507029383 27.42723435783579 22.444299956753216 14-15 23.183185252300454 24.949615701297763 26.095610163905437 25.77158888249635 16-17 22.518625109686113 28.355407579933363 26.603162588521084 22.52280472185944 18-19 22.353732359364134 26.583075095524254 29.269238295694773 21.793954249416842 20-21 23.21765823857724 25.810493635174613 29.070407937781873 21.901440188466275 22-23 22.822099785623646 25.741633762851784 29.055898657781643 22.38036779374293 24-25 22.69863409942716 26.007435244880373 29.118695337613005 22.175235318079466 26-27 22.386881801905044 26.175282575796388 29.139798311288395 22.29803731101017 28-29 22.118576878958255 26.45179358160583 29.246679873579385 22.18294966585653 30-31 22.262437950661 26.08166105354029 29.125245882960744 22.53065511283797 32-33 22.15436831898042 26.194937327020213 29.02318632649672 22.627508027502653 34-35 22.888738642613145 26.2620974917236 28.655364125839466 22.19379973982379 36-37 22.771391469236274 25.99904174629057 28.76013129643703 22.469435488036122 38-39 22.44191739670439 26.24804869930191 28.884690461239998 22.4253434427537 40-41 22.591203253557683 26.16263594036312 28.854859514367753 22.391301291711443 42-43 22.513818268331683 26.237788560668513 28.478035546653828 22.770357624345973 44-45 22.5739264355308 26.461006773611963 28.37352619760134 22.5915405932559 46-47 22.8138124394037 26.699215259948744 27.81590484897184 22.671067451675714 48-49 22.94342596850014 26.664428381881518 27.681871784098984 22.710273865519362 50-51 22.658538733571554 26.99014696774973 27.688394800078026 22.662919498600694 52-53 23.05314712306613 26.89316320806205 27.408524079418743 22.645165589453075 54-55 22.589160458277664 26.740218257101656 27.680565850037294 22.990055434583386 56-57 22.77514491622875 27.134121816527724 27.404650549574157 22.686082717669372 58-59 22.941945492795814 26.548036078189536 27.60245594216465 22.907562486849997 60-61 22.778908842623498 26.584631063233203 28.057388064893175 22.57907202925012 62-63 22.689004537528895 26.72649240072254 27.918227662052296 22.666275399696268 64-65 22.453882677448416 26.632256564940576 27.79251372756912 23.121347030041893 66-67 22.428771597746668 26.846929617012723 27.570951670674663 23.15334711456595 68-69 22.509849940484056 26.696607994855864 27.78669190760764 23.00685015705244 70-71 22.488006608645758 26.79854746320343 27.64554541391441 23.0679005142364 72-73 22.718688127202515 26.56167920898749 27.63897209696812 23.080660566841875 74-75 22.530408586518174 26.920319275456606 27.482955671022946 23.066316467002277 76-77 22.65289366526322 26.743661378208568 27.454952882990124 23.148492073538087 78-79 22.629717122484976 26.818340244141947 27.479085468123145 23.07285716524993 80-81 22.546986287065003 27.082579821536136 27.42983494086359 22.94059895053528 82-83 22.66926419901094 26.858038709379933 27.344960748827074 23.12773634278205 84-85 22.67283464612313 26.690144335791384 27.329851260704725 23.30716975738077 86-87 22.705331050820494 26.595632100430567 27.559521133417487 23.139515715331456 88-89 22.550427309434383 26.66252688914627 27.35423713454223 23.432808666877115 90-91 22.595379603106096 26.66662207996502 27.482258912977752 23.255739403951132 92-93 22.691960898711613 26.46580251497199 27.512308511840384 23.32992807447601 94-95 22.659406341085095 26.332132682972727 27.52500368404946 23.483457291892716 96-97 22.766472843636148 26.23259501625595 27.486164338007562 23.514767802100337 98-99 22.529018183998403 26.30069606745524 27.223628244404193 23.94665750414216 100-101 22.761436815929574 25.76575537975924 27.818352858714025 23.654454945597163 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2118.0 1 1227.5 2 383.0 3 437.0 4 386.0 5 392.0 6 654.5 7 1347.5 8 2225.0 9 2726.5 10 2698.5 11 2549.5 12 2739.5 13 3087.0 14 3331.0 15 3506.0 16 3714.5 17 3919.0 18 3984.0 19 4135.0 20 4466.0 21 4968.5 22 5733.5 23 6707.5 24 7955.5 25 9573.0 26 11744.0 27 14511.5 28 17178.0 29 19910.5 30 23006.5 31 26837.5 32 31830.0 33 34708.5 34 36629.0 35 39778.5 36 43458.0 37 47585.0 38 50676.0 39 52566.5 40 55232.5 41 60739.5 42 65805.5 43 70200.5 44 74743.5 45 78174.0 46 78775.0 47 80002.0 48 89835.0 49 93329.0 50 88114.5 51 84832.0 52 81232.0 53 80596.5 54 79691.0 55 73313.5 56 65544.5 57 58864.0 58 52527.5 59 45955.5 60 38759.0 61 31854.5 62 26396.5 63 21171.0 64 16229.0 65 12563.5 66 9388.0 67 7503.0 68 5681.5 69 3954.5 70 3060.0 71 2349.5 72 1896.5 73 1542.5 74 1248.0 75 1081.0 76 810.0 77 523.0 78 339.5 79 216.0 80 132.0 81 98.5 82 77.0 83 55.5 84 44.0 85 32.5 86 21.0 87 20.0 88 21.0 89 15.5 90 11.5 91 12.5 92 9.5 93 8.0 94 7.0 95 4.0 96 4.0 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33061386145884053 2 0.11477863235821215 3 0.1078946680024617 4 0.11505399093244215 5 0.13295229825739327 6 0.08439740300150024 7 0.09233690855846574 8 0.09779818694736107 9 0.08646259230822537 10-11 0.08499401324566529 12-13 0.08887197983273802 14-15 0.08992752103395309 16-17 0.07992282617026246 18-19 0.09375959452532082 20-21 0.10316767914484641 22-23 0.11156611565886193 24-25 0.12485216686546026 26-27 0.12402609114277022 28-29 0.1189319575195149 30-31 0.1093403005171693 32-33 0.1028693740227639 34-35 0.32067800623870746 36-37 0.4795369570215748 38-39 0.45484647153228325 40-41 0.4320146564190444 42-43 0.4639333044818738 44-45 0.47118441360326435 46-47 0.4784125761768022 48-49 0.4887614692582804 50-51 0.4775635539062597 52-53 0.4817168790675624 54-55 0.46831609512170164 56-57 0.4454613334606102 58-59 0.426828736604379 60-61 0.38807201728150414 62-63 0.45686576774330334 64-65 0.4874535160306878 66-67 0.4961502576667858 68-69 0.5080595160022341 70-71 0.5006018879501711 72-73 0.49188219976622055 74-75 0.49933982781828357 76-77 0.4766686385400122 78-79 0.45484647153228325 80-81 0.4573935383439109 82-83 0.47049601716768924 84-85 0.47038128442842675 86-87 0.47336433564925195 88-89 0.48570957839389767 90-91 0.5009919792636637 92-93 0.4939014959772407 94-95 0.49777946256431344 96-97 0.4978483022078709 98-99 0.4921805048883031 100-101 0.47875677439458975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2178977.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.40384201954094 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9379674397682 39.27037406979116 2 13.873571047271133 14.540568508161469 3 4.854697870602376 7.632144607909462 4 2.1366192614598627 4.478682329338035 5 1.156925333003434 3.031366618955837 6 0.6978950320165145 2.1943428602409534 7 0.45623908514517664 1.6736076658761405 8 0.3185806852263615 1.3355881519263477 9 0.21710413858769712 1.023938188230438 >10 1.1723485545865029 11.354028317280864 >50 0.10437823599870857 3.810256944237252 >100 0.06786692313670983 6.5578814219708095 >500 0.004838660998014183 1.6891079016475228 >1k 7.917808905841289E-4 0.7126082437841995 >5k 1.759513090186953E-4 0.6955041706494425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8295 0.38068322887299866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6791 0.31166001293267437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4255 0.19527512222478713 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2571 0.11799114905756233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.2946547852501426E-5 16-17 0.0 0.0 0.0 4.589309570500285E-5 4.589309570500285E-5 18-19 0.0 0.0 0.0 1.3767928711500856E-4 4.589309570500285E-5 20-21 0.0 0.0 0.0 1.3767928711500856E-4 4.589309570500285E-5 22-23 0.0 0.0 0.0 1.835723828200114E-4 6.883964355750428E-5 24-25 0.0 0.0 0.0 5.048240527550313E-4 9.17861914100057E-5 26-27 0.0 0.0 0.0 0.0011932204883300741 9.17861914100057E-5 28-29 0.0 0.0 0.0 0.0033501959864652084 9.17861914100057E-5 30-31 0.0 0.0 0.0 0.009545763906640593 1.1473273926250713E-4 32-33 0.0 0.0 0.0 0.020514213780136275 1.3767928711500856E-4 34-35 0.0 0.0 0.0 0.03756349883454484 1.835723828200114E-4 36-37 0.0 0.0 0.0 0.06652204222440164 1.835723828200114E-4 38-39 0.0 0.0 0.0 0.10803234728957671 1.835723828200114E-4 40-41 0.0 0.0 0.0 0.15452205323874463 1.835723828200114E-4 42-43 0.0 0.0 0.0 0.20241149860691507 1.835723828200114E-4 44-45 0.0 0.0 0.0 0.26312806422463386 1.835723828200114E-4 46-47 0.0 0.0 0.0 0.338094436058756 1.835723828200114E-4 48-49 0.0 0.0 0.0 0.42079379451917115 2.2946547852501425E-4 50-51 0.0 0.0 0.0 0.5128094514077018 2.2946547852501425E-4 52-53 0.0 0.0 0.0 0.6034024223293775 2.2946547852501425E-4 54-55 0.0 0.0 0.0 0.7047802707417288 2.2946547852501425E-4 56-57 0.0 0.0 0.0 0.8286686825973839 2.2946547852501425E-4 58-59 0.0 0.0 0.0 0.9682066400884451 2.2946547852501425E-4 60-61 0.0 0.0 0.0 1.1290160474387751 2.2946547852501425E-4 62-63 0.0 0.0 0.0 1.2892517910927925 2.2946547852501425E-4 64-65 0.0 0.0 0.0 1.4572893610166606 2.2946547852501425E-4 66-67 0.0 0.0 0.0 1.658530585683098 2.2946547852501425E-4 68-69 0.0 0.0 0.0 1.8867110575283723 2.2946547852501425E-4 70-71 0.0 0.0 0.0 2.1402474647506606 2.2946547852501425E-4 72-73 0.0 0.0 0.0 2.4038803530280495 2.753585742300171E-4 74-75 0.0 0.0 0.0 2.675705158888781 2.753585742300171E-4 76-77 0.0 0.0 0.0 2.97575421860809 2.753585742300171E-4 78-79 0.0 0.0 0.0 3.3209161914054164 2.753585742300171E-4 80-81 0.0 0.0 0.0 3.70302210624527 2.753585742300171E-4 82-83 0.0 0.0 0.0 4.103599992106387 2.753585742300171E-4 84-85 0.0 0.0 0.0 4.506931463709805 2.753585742300171E-4 86-87 0.0 0.0 0.0 4.930134645753489 2.9830512208251853E-4 88-89 0.0 2.2946547852501426E-5 0.0 5.387826489219482 3.2125166993501994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12160 0.0 30.215208 1 GGTATCA 8450 0.0 28.93135 1 TATCAAC 14745 0.0 24.709887 2 TCAACGC 14805 0.0 24.481735 4 CAACGCA 14935 0.0 24.268637 5 ATCAACG 14910 0.0 24.245777 3 AACGCAG 15215 0.0 23.937235 6 GTACATG 10280 0.0 23.734943 1 TACATGG 10270 0.0 22.790071 2 GAGTACT 7885 0.0 22.374025 12-13 ACATGGG 10265 0.0 21.46264 3 ACGCAGA 16930 0.0 21.456947 7 AGTACTT 8130 0.0 21.379381 12-13 CGCAGAG 17155 0.0 21.120794 8 GTACTTT 8525 0.0 20.668695 14-15 GCAGAGT 18035 0.0 19.773724 9 AGAGTAC 14425 0.0 19.764929 10-11 GTAAGGT 960 0.0 18.754332 4 CATGGGG 5350 0.0 18.686066 4 TACTTTT 9525 0.0 18.29984 14-15 >>END_MODULE