FastQCFastQC Report
Fri 27 May 2016
SRR939211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178977
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.3878425518029791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.337084787953246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.2040865966001477No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27550.12643547866728286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.028.8518331
GTATCAA126700.028.7404671
TATCAAC156500.023.1010762
TCAACGC157800.023.0009634
ATCAACG157900.022.8962523
CAACGCA159750.022.72025
AACGCAG162600.022.292796
CGTAAGA3450.022.0035342
GTACATG104300.021.9061431
GAGTACT87200.021.52045612-13
TACATGG103900.021.5078472
AGTACTT89400.021.0970212-13
ACCGTCC2257.247036E-821.0867218
ACATGGG103450.020.9134623
GTACTTT91450.020.3387314-15
ACGCAGA181250.019.9989387
TCGCACG2150.019.8607522-23
CGCAGAG182900.019.7147588
AGAGTAC156050.018.82124510-11
GCAGAGT192950.018.5653749
Produced by FastQC (version 0.11.5)