##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2178977 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49485790809173 34.0 31.0 34.0 31.0 34.0 2 32.70360953787029 34.0 31.0 34.0 31.0 34.0 3 32.787654022965825 34.0 31.0 34.0 31.0 34.0 4 36.25180945003091 37.0 35.0 37.0 35.0 37.0 5 36.21155064968561 37.0 37.0 37.0 35.0 37.0 6 36.25367821688802 37.0 37.0 37.0 35.0 37.0 7 36.208403760113114 37.0 37.0 37.0 35.0 37.0 8 36.19506952115603 37.0 37.0 37.0 35.0 37.0 9 37.93568725140284 39.0 38.0 39.0 35.0 39.0 10-11 37.8768130641122 39.0 38.0 39.0 35.0 39.0 12-13 37.80935939204498 39.0 38.0 39.0 35.0 39.0 14-15 39.17131617268103 40.5 39.0 41.0 36.0 41.0 16-17 39.111824034856724 40.0 38.0 41.0 36.0 41.0 18-19 39.14361280545871 40.5 38.5 41.0 36.0 41.0 20-21 39.21861199085626 41.0 39.0 41.0 36.0 41.0 22-23 39.18046151932765 41.0 39.0 41.0 36.0 41.0 24-25 39.12106093822927 41.0 39.0 41.0 35.5 41.0 26-27 38.9896866740677 40.0 38.5 41.0 35.0 41.0 28-29 38.8345154629902 40.0 38.0 41.0 35.0 41.0 30-31 38.63739107847398 40.0 38.0 41.0 34.5 41.0 32-33 38.49109192065818 40.0 38.0 41.0 34.0 41.0 34-35 38.43364638543684 40.0 38.0 41.0 34.0 41.0 36-37 38.32389465331667 40.0 38.0 41.0 34.0 41.0 38-39 38.18777940290329 40.0 38.0 41.0 34.0 41.0 40-41 38.009604048138186 40.0 38.0 41.0 33.0 41.0 42-43 37.83299961403907 40.0 37.5 41.0 33.0 41.0 44-45 37.58824049083584 40.0 37.0 41.0 33.0 41.0 46-47 37.26141051511787 40.0 37.0 41.0 32.0 41.0 48-49 36.91493439352504 40.0 36.5 41.0 31.0 41.0 50-51 36.63859003559927 40.0 36.0 41.0 31.0 41.0 52-53 36.39939912169794 39.0 36.0 41.0 30.0 41.0 54-55 36.14660572369511 39.0 35.0 41.0 30.0 41.0 56-57 35.86727900294496 39.0 35.0 40.5 29.0 41.0 58-59 35.56033932437102 38.5 35.0 40.0 28.5 41.0 60-61 35.20024327930033 38.0 35.0 40.0 28.0 41.0 62-63 34.80348278113996 37.5 34.0 40.0 27.0 41.0 64-65 34.38540769361035 37.0 34.0 40.0 26.0 41.0 66-67 34.02269849567021 36.5 34.0 39.0 26.0 41.0 68-69 33.55433926103855 36.0 33.0 39.0 26.0 40.0 70-71 33.11064159924589 35.0 33.0 38.5 25.0 40.0 72-73 32.64580580703697 35.0 32.5 37.5 23.5 40.0 74-75 32.160991832405756 35.0 32.0 37.0 22.5 39.0 76-77 30.18860249557476 33.5 29.5 35.0 20.0 37.5 78-79 31.166689689703013 34.5 31.0 36.0 20.0 38.0 80-81 31.197832744448426 35.0 31.0 36.0 20.0 37.0 82-83 30.917268057441635 34.5 31.0 35.0 19.0 37.0 84-85 30.55636957159254 34.0 31.0 35.0 17.5 36.5 86-87 30.01525968378739 34.0 30.5 35.0 9.5 36.0 88-89 29.487441583825806 34.0 30.0 35.0 4.5 36.0 90-91 29.043092010608646 34.0 29.0 35.0 2.0 35.0 92-93 28.765625337027423 34.0 29.0 35.0 2.0 35.0 94-95 28.437935095230465 34.0 29.0 35.0 2.0 35.0 96-97 27.894315314021213 34.0 29.0 35.0 2.0 35.0 98-99 27.12744856875497 33.5 27.0 35.0 2.0 35.0 100-101 23.521536482486965 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14974839336889545 1101 2 0.20669676433040252 1101 3 0.1717257686158149 1101 4 0.16154795125708432 1101 5 0.14075363349206071 1101 6 0.1553822638211244 1101 7 0.16451434913643226 1101 8 0.17888232464809306 1101 9 0.3172259530510999 1101 10-11 0.21563490073092595 1101 12-13 0.22522701152941949 1101 14-15 0.29895073410857265 1101 16-17 0.34010012890286845 1101 18-19 0.3347276701267319 1101 20-21 0.30120449230006585 1101 22-23 0.260516607789647 1101 24-25 0.21080104318053117 1101 26-27 0.2186056966288774 1101 28-29 0.07702738652178454 1101 30-31 0.16959188222577382 1101 32-33 0.17567522320623397 1101 34-35 0.29479783138928184 1101 36-37 0.34429843509028757 1101 38-39 0.4454535511523119 1101 40-41 0.3250703862483846 1101 42-43 0.2757436919343448 1101 44-45 0.309967518418631 1101 46-47 0.1936362869193573 1101 48-49 0.18032868751431863 1101 50-51 0.21892515430727855 1101 52-53 0.28096252647812037 1101 54-55 0.13616565089615307 1101 56-57 0.13042674622745665 1101 58-59 0.19019540756173114 1101 60-61 0.16696904058293427 1101 62-63 0.13656540087528413 1101 64-65 0.1421138024484847 1101 66-67 0.16008377896611137 1101 68-69 0.13299301215122483 1101 70-71 0.11963012996488942 1101 72-73 0.08906008890539852 1101 74-75 0.23230019646027955 1101 76-77 0.25556612513639365 1101 78-79 0.15096170597240288 1101 80-81 0.20594920541885742 1101 82-83 0.1991874653730612 1101 84-85 0.21915059894094924 1101 86-87 0.1743628813267648 1101 88-89 0.38540405790792676 1101 90-91 0.48944522106164357 1101 92-93 0.47520690190492587 1101 94-95 0.3397899860660445 1101 96-97 0.3127619990665238 1101 98-99 0.32906926426787564 1101 100-101 -0.08729243084872351 1102 1 0.26477614938121974 1102 2 0.19338929534939098 1102 3 0.2065398646694092 1102 4 0.10832636400882478 1102 5 0.06309039907393554 1102 6 0.1539118527359591 1102 7 0.18593366882402762 1102 8 0.20758518004164017 1102 9 0.14384812978997985 1102 10-11 0.19801400486874599 1102 12-13 0.1941100793119972 1102 14-15 0.32182623598789206 1102 16-17 0.27058134731610295 1102 18-19 0.23039189144984817 1102 20-21 0.2301415304136114 1102 22-23 0.2517342032116474 1102 24-25 0.32157947140446197 1102 26-27 0.2599222588398149 1102 28-29 0.0971172026126439 1102 30-31 0.11755419416952861 1102 32-33 0.17795723379584416 1102 34-35 0.2668227217301009 1102 36-37 0.34885154284638276 1102 38-39 0.3120496627687288 1102 40-41 0.2554692704821022 1102 42-43 0.21157925676339318 1102 44-45 0.16473541339337316 1102 46-47 0.0898435546513241 1102 48-49 0.1335451910414207 1102 50-51 0.13486458176975447 1102 52-53 0.14866849178282848 1102 54-55 0.12299342547510861 1102 56-57 0.23345622959981682 1102 58-59 0.1687635167158561 1102 60-61 0.013964920558485971 1102 62-63 0.08331245480361815 1102 64-65 0.11841122886990973 1102 66-67 0.1752079875357211 1102 68-69 0.07672561283929014 1102 70-71 -0.06595373540933025 1102 72-73 -0.24057032456690308 1102 74-75 -0.11895813504033725 1102 76-77 0.15825223629969187 1102 78-79 0.114353802333234 1102 80-81 0.029706953949276027 1102 82-83 -0.001455404321220044 1102 84-85 0.020008255853866785 1102 86-87 0.2080417050863126 1102 88-89 0.325067471361443 1102 90-91 0.3921037033056436 1102 92-93 0.3201284026194102 1102 94-95 0.2890272344909235 1102 96-97 0.26020682135179385 1102 98-99 0.1511525621440981 1102 100-101 -0.17387220824804572 1103 1 0.06375867779969724 1103 2 0.0863537461696069 1103 3 0.08002346362902557 1103 4 0.12494050480573549 1103 5 0.12021748727894277 1103 6 0.11217918063715615 1103 7 0.13499192944198057 1103 8 0.15379900767245402 1103 9 0.15977456758123765 1103 10-11 0.19036387251310316 1103 12-13 0.14716393947940531 1103 14-15 0.22867231543097688 1103 16-17 0.18379405016926142 1103 18-19 0.15443598294590544 1103 20-21 0.24587767630105617 1103 22-23 0.17382485217466837 1103 24-25 0.13874701023130598 1103 26-27 0.1705770937261164 1103 28-29 0.26291595254733124 1103 30-31 0.27105597628304423 1103 32-33 0.2811781927980803 1103 34-35 0.26042334424321467 1103 36-37 0.33687163979314505 1103 38-39 0.32144278939863824 1103 40-41 0.3711647588798712 1103 42-43 0.39790844770056566 1103 44-45 0.5471086279315216 1103 46-47 0.5588271677999543 1103 48-49 0.7027671530875352 1103 50-51 0.6528402047801478 1103 52-53 0.6811533751796404 1103 54-55 0.6675208027116426 1103 56-57 0.5959756997104648 1103 58-59 0.6467816855481345 1103 60-61 0.567864074601566 1103 62-63 0.5388277772137968 1103 64-65 0.47415219636964423 1103 66-67 0.5301080902535631 1103 68-69 0.3940516177463067 1103 70-71 0.27169862740611705 1103 72-73 0.41757219306261106 1103 74-75 0.22179989773124476 1103 76-77 0.20547658687387127 1103 78-79 0.3068061491296845 1103 80-81 0.25494626425185274 1103 82-83 0.3085094201872103 1103 84-85 0.2898354124578688 1103 86-87 0.528709951905121 1103 88-89 0.4125117065511006 1103 90-91 0.4408251936665266 1103 92-93 0.45842672356852177 1103 94-95 0.13063528196868646 1103 96-97 0.09981700275103123 1103 98-99 0.1295689515945817 1103 100-101 0.03885716643021553 1104 1 0.08588358096723425 1104 2 0.12619980040097545 1104 3 0.17776060096956314 1104 4 0.06362534807664844 1104 5 0.13322969368357462 1104 6 0.16948012099874177 1104 7 0.09849157455182933 1104 8 0.1818110648545357 1104 9 0.1525122304446711 1104 10-11 0.16237335528178676 1104 12-13 0.13516047751623006 1104 14-15 0.1458983071445843 1104 16-17 0.25392709180494677 1104 18-19 0.20644990346348635 1104 20-21 0.19592043180575303 1104 22-23 0.20557941092358334 1104 24-25 0.1838227309437812 1104 26-27 0.17135032605524003 1104 28-29 0.12679488245296255 1104 30-31 0.10609096572717647 1104 32-33 0.2153665064664807 1104 34-35 0.3088391846711076 1104 36-37 0.37607863223210103 1104 38-39 0.2604894000354818 1104 40-41 0.3125686965385839 1104 42-43 0.3203781198391624 1104 44-45 0.3675188991143088 1104 46-47 0.2779501025274058 1104 48-49 0.1798220449271355 1104 50-51 0.30566498766133776 1104 52-53 0.3933740532728578 1104 54-55 0.311052913458127 1104 56-57 0.4755859304085277 1104 58-59 0.4699920205722208 1104 60-61 0.484569733769483 1104 62-63 0.4214151924326828 1104 64-65 0.3750912603679524 1104 66-67 0.45972461425049715 1104 68-69 0.30924948036034294 1104 70-71 0.3098778008688967 1104 72-73 0.23906062961253127 1104 74-75 0.3676600100244585 1104 76-77 0.3962390622034917 1104 78-79 0.5128068420413534 1104 80-81 0.3819316702864093 1104 82-83 0.5247683200147613 1104 84-85 0.45808773092215205 1104 86-87 0.5502108452940426 1104 88-89 0.6205592138208331 1104 90-91 0.7042058500121868 1104 92-93 0.7514240336698492 1104 94-95 0.670824548066232 1104 96-97 0.6270258533351409 1104 98-99 0.6252002343505509 1104 100-101 0.43655342976778755 1105 1 -0.0026518800799806286 1105 2 0.030662996770381312 1105 3 0.046574380061002785 1105 4 0.020828333410328526 1105 5 0.042763567113183854 1105 6 -0.00879044783800964 1105 7 0.03836706653461874 1105 8 0.07551189848139472 1105 9 0.029177314598612725 1105 10-11 0.037850406294850814 1105 12-13 0.09874213772523177 1105 14-15 0.16641137308432263 1105 16-17 0.10069047041856294 1105 18-19 0.10942668647036413 1105 20-21 0.019751541963948682 1105 22-23 0.1034454435641905 1105 24-25 0.020715569307974135 1105 26-27 0.06543160084581956 1105 28-29 -0.028942491903187317 1105 30-31 0.03731151695208723 1105 32-33 0.07385349668042096 1105 34-35 0.16335036691020122 1105 36-37 0.11456814936152426 1105 38-39 0.12472397436508231 1105 40-41 0.02120296253344378 1105 42-43 0.07873021360559562 1105 44-45 0.07930147207579807 1105 46-47 0.0674905867763087 1105 48-49 0.10623533088718773 1105 50-51 0.10674518021740198 1105 52-53 -0.02862759560329664 1105 54-55 0.035504394128501815 1105 56-57 0.08460612531283118 1105 58-59 -0.08250707331161777 1105 60-61 0.006431764184348765 1105 62-63 0.13573935740197385 1105 64-65 0.06618426617220052 1105 66-67 0.10177070110491826 1105 68-69 -0.07820668148476528 1105 70-71 -0.019317134069410713 1105 72-73 -0.07325323370941561 1105 74-75 -0.05551001820552415 1105 76-77 -0.09108781856183512 1105 78-79 0.08476165364576715 1105 80-81 0.0910252478371909 1105 82-83 0.05285064641056181 1105 84-85 0.1502303000039369 1105 86-87 0.24768980042725275 1105 88-89 0.42353076432596026 1105 90-91 0.3004184121002851 1105 92-93 0.2753328656223104 1105 94-95 0.24926332269089357 1105 96-97 0.18840922469982502 1105 98-99 0.06057717867162893 1105 100-101 -0.4000891418664345 1106 1 0.037211536898773545 1106 2 0.07137278598316499 1106 3 0.06600994753093659 1106 4 0.05578970545839468 1106 5 0.09180308959712846 1106 6 0.048668451849479766 1106 7 0.09628253039905132 1106 8 0.07021217780807376 1106 9 0.0672082760870083 1106 10-11 0.10567736584922471 1106 12-13 0.10586394284886325 1106 14-15 0.08108000524188697 1106 16-17 0.06866529477844807 1106 18-19 0.017526039603559695 1106 20-21 0.15331623293556618 1106 22-23 0.09529583260931673 1106 24-25 0.18449443775359953 1106 26-27 0.1634275582859246 1106 28-29 -0.0024257690183375757 1106 30-31 0.013375752551596065 1106 32-33 0.2367964831394218 1106 34-35 0.038085130541091416 1106 36-37 0.13356717949047692 1106 38-39 0.3062132414114984 1106 40-41 0.3126586321783904 1106 42-43 0.36147027576507895 1106 44-45 0.3375394156821443 1106 46-47 0.42558507624934094 1106 48-49 0.27149737726743695 1106 50-51 0.22058868289193612 1106 52-53 0.41052646607744947 1106 54-55 0.35135251155823966 1106 56-57 0.3771260782402379 1106 58-59 0.2872231270616439 1106 60-61 0.34472543484589124 1106 62-63 0.3539578415993816 1106 64-65 0.5652601707414675 1106 66-67 0.4824839520993507 1106 68-69 0.3243895832242316 1106 70-71 0.3373306549202155 1106 72-73 0.17485220376727995 1106 74-75 0.16195471787415272 1106 76-77 0.31014817099751113 1106 78-79 0.46745398517954584 1106 80-81 0.318661947681651 1106 82-83 0.41496814576446184 1106 84-85 0.4190500422680934 1106 86-87 0.4098859527952392 1106 88-89 0.6369977624844481 1106 90-91 0.7693333056767244 1106 92-93 0.6797959175404529 1106 94-95 0.38730806454974953 1106 96-97 0.4339790024877743 1106 98-99 0.4137896500499423 1106 100-101 0.08470834390735504 1107 1 0.019090761803880696 1107 2 0.04124299284759303 1107 3 0.10055601217249688 1107 4 0.07955481109080154 1107 5 0.057263469043434156 1107 6 0.04159721614039569 1107 7 0.060799081118162235 1107 8 0.05706324665210616 1107 9 -0.004881734932382642 1107 10-11 0.056746140837333314 1107 12-13 0.08288800783471828 1107 14-15 0.057036192809029274 1107 16-17 0.09538605035695724 1107 18-19 0.09179576334550887 1107 20-21 0.16282954780198367 1107 22-23 0.037552662652025504 1107 24-25 0.03237000609913565 1107 26-27 0.08037281668004681 1107 28-29 0.020450052487795745 1107 30-31 0.08541329028403766 1107 32-33 0.18399592857963398 1107 34-35 0.2589914546768668 1107 36-37 0.1903544380558131 1107 38-39 0.17380932966604234 1107 40-41 0.17146602023307622 1107 42-43 0.20640549887320958 1107 44-45 0.21062264475934 1107 46-47 0.15582980429502413 1107 48-49 0.13420251790044802 1107 50-51 0.16941031238081905 1107 52-53 0.15807414542332054 1107 54-55 0.03853013455516674 1107 56-57 0.016541387735962587 1107 58-59 0.16439363394438544 1107 60-61 0.11912689415562028 1107 62-63 -0.02504600828283543 1107 64-65 0.06370944702437242 1107 66-67 0.2520784670758687 1107 68-69 0.20083927284934333 1107 70-71 0.21891593735998782 1107 72-73 0.17797263824609644 1107 74-75 0.13524719569038268 1107 76-77 -0.04165633470872976 1107 78-79 0.25886864198123405 1107 80-81 0.1461303555408584 1107 82-83 0.19187750598620923 1107 84-85 0.20013482621163803 1107 86-87 0.2117212750502624 1107 88-89 0.48116982147178433 1107 90-91 0.6195503793796 1107 92-93 0.6392726161789994 1107 94-95 0.4928296582279117 1107 96-97 0.34435888607072584 1107 98-99 0.4326394125853028 1107 100-101 0.042527070794943 1108 1 -0.016497086862678145 1108 2 -0.043667780926554656 1108 3 -0.006275061243307789 1108 4 -0.008581072077760155 1108 5 -0.03887036129168564 1108 6 -0.05399909596231822 1108 7 -0.04378918171654789 1108 8 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-0.7739295684984704 2316 90-91 -0.8162191797256142 2316 92-93 -0.6994561676264013 2316 94-95 -0.24984280011126003 2316 96-97 -0.48027508192571844 2316 98-99 -0.5068157723843782 2316 100-101 -0.08849960968905535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 12.0 4 16.0 5 45.0 6 83.0 7 213.0 8 506.0 9 971.0 10 1618.0 11 2658.0 12 3990.0 13 5949.0 14 7407.0 15 9901.0 16 13304.0 17 19492.0 18 19472.0 19 12283.0 20 10648.0 21 11109.0 22 11400.0 23 12402.0 24 14398.0 25 16807.0 26 19861.0 27 23953.0 28 28923.0 29 35510.0 30 44016.0 31 54040.0 32 67499.0 33 88215.0 34 122920.0 35 183203.0 36 301838.0 37 523064.0 38 466805.0 39 44444.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10016683804829 19.18660580124859 14.82339711369716 22.889830247005964 2 15.689013697712276 20.34674987390872 40.02506680887407 23.939169619504934 3 19.496717955260657 24.379330300411613 29.748271780748485 26.37567996357924 4 13.307988106345316 16.80637289884198 36.10028926418223 33.78534973063048 5 14.089272167627287 37.23986072363316 33.55937212737904 15.111494981360519 6 32.37510079271144 35.459988792906024 17.10265872471348 15.06225168966905 7 28.99277963925273 32.85027790564104 20.008242399988617 18.148700055117608 8 26.453881798660568 35.07154963085888 19.273448044655815 19.201120525824734 9 26.650120675895156 17.18407307649415 20.30870449756927 35.85710175004142 10-11 25.506923661883533 26.47533682090265 26.43504268287366 21.58269683434015 12-13 26.336992083899922 23.80775473995366 27.408251670393952 22.44700150575247 14-15 23.023215256854776 25.074542613316936 26.038949863581394 25.863292266246894 16-17 22.445098739773957 28.478827610467413 26.581478240855795 22.494595408902835 18-19 22.209537874671405 26.6622257427342 29.299542537412073 21.828693845182322 20-21 23.081725965900514 25.78173151896509 29.239455028667123 21.897087486467274 22-23 22.720684981989255 25.749193314110247 29.2176787547551 22.312442949145403 24-25 22.59440648178255 26.073896892038594 29.250262222698492 22.081434403480362 26-27 22.34075289067181 26.203143396918282 29.24878674277983 22.207316969630075 28-29 22.014619704567785 26.46347345566291 29.37031919106994 22.151587648699365 30-31 22.240643733529637 26.07711915201793 29.210170754788855 22.472066359663575 32-33 22.115263263448856 26.267211631880468 29.067057614651283 22.5504674900194 34-35 22.775904512505182 26.293622072957675 28.787842973732953 22.142630440804194 36-37 22.693396193934444 25.94223190328526 28.902387800024332 22.461984102755967 38-39 22.389778669113607 26.28020250603281 28.949787996619698 22.380230828233884 40-41 22.535689655765943 26.148408839766326 28.959885067890955 22.356016436576777 42-43 22.446497356852962 26.289875260901045 28.514020096408604 22.74960728583739 44-45 22.49634164503321 26.494819787724772 28.466097714162064 22.54274085307996 46-47 22.79720315023074 26.69107137079462 27.877720086931717 22.634005392042916 48-49 22.89283939194929 26.745497743651452 27.72752451510191 22.634138349297352 50-51 22.6168674223276 26.994658728379463 27.726493482831728 22.661980366461208 52-53 23.02396769123097 26.945100333872578 27.404490643536526 22.626441331359928 54-55 22.503243494538953 26.797758764778152 27.679548705654078 23.019449035028824 56-57 22.738445610027092 27.165201835540252 27.390766400930346 22.705586153502306 58-59 22.87662075851372 26.62554308303878 27.55824725506849 22.939588903379008 60-61 22.677646667881433 26.69497496040159 28.00182581839395 22.62555255332303 62-63 22.647647699331422 26.811741366827647 27.83189968229054 22.708711251550397 64-65 22.36403998107329 26.712846804235657 27.727312154446377 23.19580106024468 66-67 22.36508309859963 26.923831542472936 27.538732495367473 23.172352863559958 68-69 22.390949709978674 26.770539056212755 27.72737880706772 23.111132426740845 70-71 22.49176275731416 26.821136814066975 27.53172305058042 23.15537737803844 72-73 22.569513124736975 26.650166568990862 27.528468634593207 23.251851671678956 74-75 22.365151169562598 27.010220851344464 27.344414374268293 23.28021360482465 76-77 22.42952355240022 26.829848815929452 27.4637263554985 23.276901276171824 78-79 22.42489967156478 26.86128780659289 27.479653790314085 23.234158731528243 80-81 22.30015145256781 27.079374913947408 27.48556611134059 23.134907522144204 82-83 22.44208200730209 26.886601410899537 27.369009453802484 23.30230712799589 84-85 22.35978680816429 26.697327850942866 27.359473587714234 23.583411753178616 86-87 22.371578184457867 26.686744151054352 27.493013655675735 23.448664008812038 88-89 22.18707172330019 26.734558076048575 27.25544853214281 23.82292166850842 90-91 22.17123008065876 26.797820471825645 27.432341278800283 23.598608168715312 92-93 22.18050029899352 26.633392642510685 27.424153628055738 23.761953430440062 94-95 22.07526743054195 26.49403825740244 27.45696719148481 23.9737271205708 96-97 22.14135348835715 26.36175140903277 27.45859639638234 24.03829870622774 98-99 21.83359798801252 26.460572571984542 27.17307498141298 24.532754458589956 100-101 21.935624660531158 25.847453057210206 27.905824602659397 24.31109767959924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 487.0 1 333.5 2 242.0 3 317.0 4 291.5 5 292.0 6 542.5 7 1195.5 8 1988.0 9 2410.0 10 2345.0 11 2248.5 12 2408.0 13 2649.0 14 2870.5 15 3092.5 16 3206.5 17 3349.0 18 3533.0 19 3750.5 20 4122.5 21 4732.0 22 5612.0 23 6641.0 24 7944.0 25 9679.5 26 11993.5 27 14574.5 28 17225.0 29 20317.5 30 23300.0 31 26937.0 32 31969.5 33 34943.5 34 36900.5 35 39959.0 36 43722.5 37 47961.5 38 50990.0 39 52678.5 40 55508.5 41 61442.5 42 66350.5 43 70468.5 44 74955.5 45 78403.5 46 79112.5 47 80877.5 48 90976.5 49 94038.5 50 88906.5 51 86005.0 52 82094.0 53 81235.0 54 80078.5 55 73393.5 56 65282.0 57 58479.5 58 52392.0 59 45785.5 60 38674.0 61 31741.0 62 26371.0 63 20956.0 64 15867.5 65 12275.0 66 9060.0 67 7250.0 68 5490.5 69 3771.5 70 2824.5 71 2079.0 72 1744.0 73 1516.0 74 1227.0 75 1065.0 76 752.5 77 421.5 78 231.0 79 146.5 80 93.0 81 49.5 82 32.5 83 15.5 84 6.5 85 5.5 86 4.5 87 2.5 88 2.0 89 2.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34006783917407113 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010096481055100626 16-17 0.001950456567462621 18-19 4.589309570500285E-5 20-21 0.0 22-23 0.0 24-25 3.900913134925243E-4 26-27 0.0013079532275925812 28-29 0.0 30-31 0.0028912650294151796 32-33 0.0 34-35 0.010119427602953129 36-37 0.028086574571461742 38-39 0.021753327364171352 40-41 0.013744982163648353 42-43 0.010532465464298155 44-45 0.0027765322901526722 46-47 0.0011702739404775729 48-49 6.4250333987004E-4 50-51 6.883964355750428E-5 52-53 9.17861914100057E-5 54-55 0.0 56-57 0.0 58-59 0.004474576831237778 60-61 0.01046362582074065 62-63 0.004566363022647783 64-65 0.002111082402430131 66-67 0.004107432065597755 68-69 0.003923859682777744 70-71 6.883964355750428E-5 72-73 0.0 74-75 0.0 76-77 0.21753327364171351 78-79 3.900913134925243E-4 80-81 0.0035337683692852192 82-83 5.736636963125356E-4 84-85 2.2946547852501426E-5 86-87 0.11057941410120437 88-89 0.4104219548898405 90-91 0.3946347299673195 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0016980445410851054 100-101 9.867015576575614E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2178977.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.94897157115808 #Duplication Level Percentage of deduplicated Percentage of total 1 73.81947396950571 37.610262806701904 2 14.376098860436382 14.648949042890628 3 5.0555767328987145 7.7272930572079455 4 2.2564102808297237 4.5984713300344975 5 1.2385928432866309 3.155251578042521 6 0.7249579511581468 2.216151722630486 7 0.495112395272915 1.7657827145901412 8 0.33255523381496593 1.3554677722782824 9 0.25007098655476423 1.1466773626275124 >10 1.2621739887308647 11.804848570550746 >50 0.1102688794437637 3.897785217219278 >100 0.07292711977742306 6.962934680630347 >500 0.004696866110440337 1.6035434327928422 >1k 9.03243482776981E-4 0.7796594300007511 >5k 1.806486965553962E-4 0.7269212818021406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8451 0.3878425518029791 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7345 0.337084787953246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4447 0.2040865966001477 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2755 0.12643547866728286 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.589309570500285E-5 2 0.0 0.0 0.0 0.0 4.589309570500285E-5 3 0.0 0.0 0.0 0.0 4.589309570500285E-5 4 0.0 0.0 0.0 0.0 4.589309570500285E-5 5 0.0 0.0 0.0 0.0 4.589309570500285E-5 6 0.0 0.0 0.0 0.0 4.589309570500285E-5 7 0.0 0.0 0.0 0.0 4.589309570500285E-5 8 0.0 0.0 0.0 0.0 4.589309570500285E-5 9 0.0 0.0 0.0 4.589309570500285E-5 4.589309570500285E-5 10-11 0.0 0.0 0.0 4.589309570500285E-5 6.883964355750428E-5 12-13 0.0 0.0 0.0 4.589309570500285E-5 9.17861914100057E-5 14-15 0.0 0.0 0.0 4.589309570500285E-5 1.3767928711500856E-4 16-17 0.0 0.0 0.0 1.3767928711500856E-4 1.3767928711500856E-4 18-19 0.0 0.0 0.0 1.3767928711500856E-4 1.835723828200114E-4 20-21 0.0 0.0 0.0 1.3767928711500856E-4 1.835723828200114E-4 22-23 0.0 0.0 0.0 1.835723828200114E-4 1.835723828200114E-4 24-25 0.0 0.0 0.0 5.507171484600342E-4 2.5241202637751566E-4 26-27 0.0 0.0 0.0 0.0012850066797400798 2.753585742300171E-4 28-29 0.0 0.0 0.0 0.003441982177875214 2.753585742300171E-4 30-31 0.0 0.0 0.0 0.009545763906640593 2.753585742300171E-4 32-33 0.0 0.0 0.0 0.020697786162956287 2.753585742300171E-4 34-35 0.0 0.0 0.0 0.03802242979159486 2.753585742300171E-4 36-37 0.0 0.0 0.0 0.06737106449494418 2.753585742300171E-4 38-39 0.0 0.0 0.0 0.10954681944784181 2.753585742300171E-4 40-41 0.0 0.0 0.0 0.1564954563540597 2.753585742300171E-4 42-43 0.0 0.0 0.0 0.20537160327988777 2.753585742300171E-4 44-45 0.0 0.0 0.0 0.2668683515245916 2.9830512208251853E-4 46-47 0.0 0.0 0.0 0.3424542801507313 3.2125166993501994E-4 48-49 0.0 0.0 0.0 0.425956767785984 3.2125166993501994E-4 50-51 0.0 0.0 0.0 0.5187067142057947 3.2125166993501994E-4 52-53 0.0 0.0 0.0 0.6101716539458655 3.2125166993501994E-4 54-55 0.0 0.0 0.0 0.7121920056980868 3.2125166993501994E-4 56-57 0.0 0.0 0.0 0.8375260500684496 3.441982177875214E-4 58-59 0.0 0.0 0.0 0.9777065108993808 3.671447656400228E-4 60-61 0.0 0.0 0.0 1.1399844973122708 3.671447656400228E-4 62-63 0.0 0.0 0.0 1.3013675683589132 3.671447656400228E-4 64-65 0.0 0.0 0.0 1.4706671984146689 3.671447656400228E-4 66-67 0.0 0.0 0.0 1.6731016435694364 3.671447656400228E-4 68-69 0.0 0.0 0.0 1.9027048013815657 3.671447656400228E-4 70-71 0.0 0.0 0.0 2.1574114825443313 3.671447656400228E-4 72-73 0.0 0.0 0.0 2.4219622327358206 3.671447656400228E-4 74-75 0.0 0.0 0.0 2.695806334807573 3.671447656400228E-4 76-77 0.0 0.0 0.0 2.997828797642196 3.671447656400228E-4 78-79 0.0 0.0 0.0 3.3439086323536227 3.671447656400228E-4 80-81 0.0 0.0 0.0 3.727001248751134 4.130378613450257E-4 82-83 0.0 0.0 0.0 4.128657622361319 4.589309570500285E-4 84-85 0.0 0.0 0.0 4.533159367905214 4.589309570500285E-4 86-87 0.0 0.0 0.0 4.957808182463605 4.589309570500285E-4 88-89 0.0 0.0 0.0 5.418092985836932 4.589309570500285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8975 0.0 28.851833 1 GTATCAA 12670 0.0 28.740467 1 TATCAAC 15650 0.0 23.101076 2 TCAACGC 15780 0.0 23.000963 4 ATCAACG 15790 0.0 22.896252 3 CAACGCA 15975 0.0 22.7202 5 AACGCAG 16260 0.0 22.29279 6 CGTAAGA 345 0.0 22.003534 2 GTACATG 10430 0.0 21.906143 1 GAGTACT 8720 0.0 21.520456 12-13 TACATGG 10390 0.0 21.507847 2 AGTACTT 8940 0.0 21.09702 12-13 ACCGTCC 225 7.247036E-8 21.086721 8 ACATGGG 10345 0.0 20.913462 3 GTACTTT 9145 0.0 20.33873 14-15 ACGCAGA 18125 0.0 19.998938 7 TCGCACG 215 0.0 19.86075 22-23 CGCAGAG 18290 0.0 19.714758 8 AGAGTAC 15605 0.0 18.821245 10-11 GCAGAGT 19295 0.0 18.565374 9 >>END_MODULE