FastQCFastQC Report
Fri 27 May 2016
SRR939210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145598
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79840.3721107122583075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66760.31114868675306373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40550.18899160047688338No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25340.11810227265312515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84400.031.2241211
GTATCAA122550.029.9118791
GTACATG102400.024.9472121
TATCAAC148250.024.8273852
TACATGG101250.024.7026522
TCAACGC149850.024.4358484
CAACGCA150250.024.3707945
ATCAACG151700.024.1066233
AACGCAG153050.023.9711786
ACATGGG102200.023.2215673
GAGTACT80000.022.60684212-13
ACGCAGA170050.021.463417
GTACTTT84450.021.36028714-15
CGCAGAG173200.020.9637248
AGTACTT82250.020.34792112-13
GCAGAGT179550.019.7741039
ATAACGC1708.127096E-519.5053543
TACCTGG17250.019.2226662
CATGGGG49650.019.1770364
AGAGTAC146900.018.99757610-11
Produced by FastQC (version 0.11.5)