##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2145598 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15751785749241 33.0 31.0 34.0 30.0 34.0 2 32.25520670694137 34.0 31.0 34.0 30.0 34.0 3 32.315078127403176 34.0 31.0 34.0 30.0 34.0 4 35.75803389078476 37.0 35.0 37.0 35.0 37.0 5 35.79365892399228 37.0 35.0 37.0 35.0 37.0 6 35.88266627765313 37.0 37.0 37.0 35.0 37.0 7 35.814042518682434 37.0 36.0 37.0 35.0 37.0 8 35.811223724108615 37.0 36.0 37.0 35.0 37.0 9 37.49550847828904 39.0 38.0 39.0 35.0 39.0 10-11 37.40123942136411 39.0 38.0 39.0 35.0 39.0 12-13 37.30704633393581 39.0 37.0 39.0 34.5 39.0 14-15 38.60840311185972 40.0 38.0 41.0 34.0 41.0 16-17 38.52154667370122 40.0 38.0 41.0 34.0 41.0 18-19 38.42609985654349 40.0 38.0 41.0 34.0 41.0 20-21 38.414847049633714 40.0 38.0 41.0 34.0 41.0 22-23 38.4524204906977 40.0 38.0 41.0 34.0 41.0 24-25 38.39877344218255 40.0 38.0 41.0 34.0 41.0 26-27 38.291446021109266 40.0 38.0 41.0 34.0 41.0 28-29 38.15210980808148 40.0 38.0 41.0 33.5 41.0 30-31 37.93688379649869 40.0 38.0 41.0 33.0 41.0 32-33 37.734754366847845 40.0 37.5 41.0 33.0 41.0 34-35 37.42795108869416 40.0 37.0 41.0 32.5 41.0 36-37 37.16998291385432 40.0 37.0 41.0 31.0 41.0 38-39 36.98916129675736 40.0 37.0 41.0 31.0 41.0 40-41 36.79366172041547 40.0 36.5 41.0 30.5 41.0 42-43 36.56948762070061 40.0 36.0 41.0 30.0 41.0 44-45 36.28379384208971 39.5 36.0 41.0 30.0 41.0 46-47 36.09955056818659 40.0 36.0 41.0 29.0 41.0 48-49 35.95226878473973 40.0 36.0 41.0 28.0 41.0 50-51 34.818358564838334 38.5 34.0 40.0 25.5 40.5 52-53 34.95302381900058 38.5 34.5 39.5 26.5 40.5 54-55 35.46823030222809 39.0 35.0 41.0 27.0 41.0 56-57 35.37442288816451 39.0 35.0 41.0 26.5 41.0 58-59 35.171075849250414 39.0 35.0 41.0 26.0 41.0 60-61 34.87311835674716 38.5 35.0 40.5 26.0 41.0 62-63 34.48779990473518 38.0 34.5 40.0 25.5 41.0 64-65 34.11036759914951 37.0 34.0 40.0 23.5 41.0 66-67 33.72082421777053 37.0 34.0 39.5 22.5 41.0 68-69 33.3140366928008 36.0 34.0 39.0 22.0 41.0 70-71 32.88219088571112 36.0 33.0 39.0 21.0 40.5 72-73 32.43428941488573 35.0 33.0 38.0 20.0 40.0 74-75 31.9956133441586 35.0 32.0 37.0 18.0 39.5 76-77 31.56256088046316 35.0 32.0 37.0 16.0 39.0 78-79 31.140702498790546 35.0 31.5 36.0 11.5 39.0 80-81 30.70090226594171 35.0 31.0 36.0 7.0 37.5 82-83 30.31424945399837 35.0 31.0 35.0 4.5 37.0 84-85 29.952594335005905 34.0 31.0 35.0 2.0 37.0 86-87 29.55416182341706 34.0 30.0 35.0 2.0 36.0 88-89 29.146085380392783 34.0 30.0 35.0 2.0 36.0 90-91 28.751418252627005 34.0 29.0 35.0 2.0 35.5 92-93 28.348510531795796 34.0 29.0 35.0 2.0 35.0 94-95 27.86845112644587 34.0 28.5 35.0 2.0 35.0 96-97 27.28141548416805 34.0 27.0 35.0 2.0 35.0 98-99 26.569311912110287 33.5 25.5 35.0 2.0 35.0 100-101 23.677668649952135 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.020506760414605196 1101 2 0.1273853365312121 1101 3 0.04966639562385211 1101 4 0.1374320363635846 1101 5 0.07572184528271464 1101 6 0.10512456888679367 1101 7 0.061607161718988834 1101 8 0.12221104189875831 1101 9 0.13633518694798852 1101 10-11 0.09406259401485784 1101 12-13 0.1142447555011259 1101 14-15 0.27745585434222164 1101 16-17 0.2109614719469235 1101 18-19 0.26044810236633253 1101 20-21 0.21923620785029385 1101 22-23 0.32208393400929225 1101 24-25 0.3497127838379015 1101 26-27 0.27639627404159484 1101 28-29 0.2223358760349754 1101 30-31 0.29036576694928584 1101 32-33 0.07760890614461147 1101 34-35 0.018806399944949703 1101 36-37 -0.0050438394112077845 1101 38-39 -0.09230864358321611 1101 40-41 -0.024098603906033134 1101 42-43 -0.05415712105553183 1101 44-45 -0.03070951294576929 1101 46-47 -0.005150566345726304 1101 48-49 -0.16182587921432656 1101 50-51 -0.16217092942590483 1101 52-53 -0.21874774364125926 1101 54-55 -0.22632347265591335 1101 56-57 -0.10540232950438622 1101 58-59 -0.15227095961154902 1101 60-61 -0.3809181193699587 1101 62-63 -0.33216463617290515 1101 64-65 -0.33863945631940595 1101 66-67 -0.29387188099197914 1101 68-69 -0.23361096178719265 1101 70-71 -0.3057828193338139 1101 72-73 -0.48007806662971575 1101 74-75 -0.7957702006913969 1101 76-77 -0.7282738629030483 1101 78-79 -0.7719478356619582 1101 80-81 -0.8430335072521657 1101 82-83 -0.8908756123393253 1101 84-85 -0.9113118488939733 1101 86-87 -1.0959205239691627 1101 88-89 -1.1062208274426553 1101 90-91 -1.1348499774888658 1101 92-93 -0.9920877981598721 1101 94-95 -1.2126396071518748 1101 96-97 -1.3831504655076259 1101 98-99 -1.3558170143952317 1101 100-101 -1.3980060934713556 1102 1 0.09575739162421826 1102 2 0.11327436617516184 1102 3 0.2675000877172664 1102 4 0.08415456029177903 1102 5 0.21470903903591676 1102 6 0.1684938132382925 1102 7 0.23664405400285204 1102 8 0.22868605346680226 1102 9 0.2520605484748657 1102 10-11 0.22585732412205317 1102 12-13 0.24521367612246792 1102 14-15 0.2825898481675395 1102 16-17 0.23170342551954093 1102 18-19 0.24039074937967797 1102 20-21 0.35791560121219135 1102 22-23 0.3963227626056067 1102 24-25 0.4191208968143556 1102 26-27 0.3471781103758147 1102 28-29 0.4218291869927384 1102 30-31 0.41884836061833397 1102 32-33 0.4063807154799193 1102 34-35 0.42512173458960945 1102 36-37 0.43632120718481104 1102 38-39 0.3828527401006241 1102 40-41 0.29095055954793736 1102 42-43 0.36233313173757153 1102 44-45 0.4042704989927941 1102 46-47 0.5606205421966592 1102 48-49 0.38653014576681244 1102 50-51 0.26204846835899076 1102 52-53 0.33680176612462276 1102 54-55 0.32809549064902654 1102 56-57 0.18802684693847027 1102 58-59 0.08570017192104018 1102 60-61 0.06712634159424624 1102 62-63 -5.209470728928522E-4 1102 64-65 -0.014848469998575808 1102 66-67 -0.0028021185438547036 1102 68-69 -0.050589785703778034 1102 70-71 -0.16695769488617884 1102 72-73 -0.054792600001405845 1102 74-75 -0.17348898258374845 1102 76-77 -0.28925510137512234 1102 78-79 -0.27550417013566175 1102 80-81 -0.2545055925283357 1102 82-83 -0.35758459305030854 1102 84-85 -0.4089306992317958 1102 86-87 -0.4802628493529788 1102 88-89 -0.5792407373140342 1102 90-91 -0.5590601850251034 1102 92-93 -0.8752387463893037 1102 94-95 -0.786250143669843 1102 96-97 -0.7303548502190225 1102 98-99 -0.8824325579940648 1102 100-101 -0.92353324905147 1103 1 0.16452560342276001 1103 2 0.2899036277791964 1103 3 0.24258870743706495 1103 4 0.2972540457850883 1103 5 0.40098314250598577 1103 6 0.29329248328050994 1103 7 0.29719961531480976 1103 8 0.22329403712784313 1103 9 0.37949392106681756 1103 10-11 0.3395927762697113 1103 12-13 0.3374665453981649 1103 14-15 0.3951956075570848 1103 16-17 0.3610484972960464 1103 18-19 0.3644911685604413 1103 20-21 0.3682489080661071 1103 22-23 0.4341609940564908 1103 24-25 0.4793336647314277 1103 26-27 0.36570622288802923 1103 28-29 0.3087108205198277 1103 30-31 0.39958199029094743 1103 32-33 0.4169707576052204 1103 34-35 0.2995612055077288 1103 36-37 0.24067655412856936 1103 38-39 0.20873787236534014 1103 40-41 0.2869412295809397 1103 42-43 0.25172887131359545 1103 44-45 0.22580868865224346 1103 46-47 0.351002812956807 1103 48-49 0.35705797276177975 1103 50-51 0.18695023545749478 1103 52-53 0.23573499077719617 1103 54-55 0.19024764601646638 1103 56-57 0.18058181188777667 1103 58-59 0.23762413222953427 1103 60-61 0.229694166332699 1103 62-63 0.029276174498740204 1103 64-65 0.1568910208366603 1103 66-67 0.15388813783452093 1103 68-69 0.06495720433420615 1103 70-71 -0.043149988453173194 1103 72-73 -0.014932146558557235 1103 74-75 -0.09804306335059465 1103 76-77 -0.039216821340257724 1103 78-79 0.09672513245390846 1103 80-81 0.09943362453270055 1103 82-83 0.01724717627017469 1103 84-85 -0.2750412404596787 1103 86-87 -0.3175007462421071 1103 88-89 -0.17002860375182394 1103 90-91 -0.1722076212989201 1103 92-93 -0.40369779365967773 1103 94-95 -0.6970415445349118 1103 96-97 -0.5015614138922473 1103 98-99 -0.5554686770777835 1103 100-101 -0.6534111904260307 1104 1 0.06951410069728325 1104 2 0.1349576241138024 1104 3 0.17028477968125344 1104 4 0.08786657643518225 1104 5 0.06917453035567434 1104 6 0.07989630989312246 1104 7 0.194984394019869 1104 8 0.1379643456230326 1104 9 0.28409871367276196 1104 10-11 0.23104684867208647 1104 12-13 0.28017716644436774 1104 14-15 0.3711160359599788 1104 16-17 0.3262306644532842 1104 18-19 0.3285335813896424 1104 20-21 0.2434657937641873 1104 22-23 0.2318674330583832 1104 24-25 0.21736350954981276 1104 26-27 0.2576252006180155 1104 28-29 0.4210062832121224 1104 30-31 0.41777640738802546 1104 32-33 0.3068470969953694 1104 34-35 0.21378974875948842 1104 36-37 0.32774175194683863 1104 38-39 0.13424741701897602 1104 40-41 0.2169576211751192 1104 42-43 0.3653870745996599 1104 44-45 0.33284672421896744 1104 46-47 0.4922490453303823 1104 48-49 0.337893355044379 1104 50-51 0.35718299946061194 1104 52-53 0.36788632861064485 1104 54-55 0.44574825439031684 1104 56-57 0.4578357823054944 1104 58-59 0.34057058018454 1104 60-61 0.288495644605419 1104 62-63 0.242395087604784 1104 64-65 0.32515813987094333 1104 66-67 0.2603106471620791 1104 68-69 0.3663113620603866 1104 70-71 0.2673133685924327 1104 72-73 0.3811756071366972 1104 74-75 0.23960358167378715 1104 76-77 0.16568567896687014 1104 78-79 0.14679059785569848 1104 80-81 0.1361220176347686 1104 82-83 0.15416873727501113 1104 84-85 0.007194579717463512 1104 86-87 0.06923569408928998 1104 88-89 0.06200248978964851 1104 90-91 0.3142495392528737 1104 92-93 0.10831275148704478 1104 94-95 0.03724844195128085 1104 96-97 -0.10303993905267106 1104 98-99 0.020678187567028772 1104 100-101 -0.27145338784208306 1105 1 0.08234856589546524 1105 2 0.22769671040713746 1105 3 0.17704131852301686 1105 4 0.19549089165894173 1105 5 0.10432671040494768 1105 6 0.14168682866917948 1105 7 0.13367658035416952 1105 8 0.18775200601719888 1105 9 0.14147281386485844 1105 10-11 0.20328823960911535 1105 12-13 0.24038704588003412 1105 14-15 0.16145009934815135 1105 16-17 0.153101586547983 1105 18-19 0.11957251151681447 1105 20-21 0.13689657968860303 1105 22-23 0.15595345851887998 1105 24-25 0.26767665482806535 1105 26-27 0.3318517063261126 1105 28-29 0.19718930387995215 1105 30-31 0.2274318689524577 1105 32-33 0.2336535695618025 1105 34-35 0.17310405162375986 1105 36-37 0.25221919234635237 1105 38-39 0.2095900616832438 1105 40-41 0.24641862534568304 1105 42-43 0.30449466453114127 1105 44-45 0.1924137875780758 1105 46-47 0.2685166926533924 1105 48-49 0.3884109369983051 1105 50-51 0.24296526827058784 1105 52-53 0.2276293087962813 1105 54-55 0.3466726973137 1105 56-57 0.28821491238612396 1105 58-59 0.2669825725945216 1105 60-61 0.3245492651203108 1105 62-63 0.30008701086346434 1105 64-65 0.47011600379287444 1105 66-67 0.5541741085175502 1105 68-69 0.45242771291636075 1105 70-71 0.32763378243409846 1105 72-73 0.35884403217200855 1105 74-75 0.3751781486851691 1105 76-77 0.41297542704065293 1105 78-79 0.43957243726869066 1105 80-81 0.3656852134891011 1105 82-83 0.4097594939652467 1105 84-85 0.2931120645972811 1105 86-87 0.30508844957316583 1105 88-89 0.33104102170509364 1105 90-91 0.3208270960506887 1105 92-93 0.4609672164560088 1105 94-95 0.3534925217503506 1105 96-97 0.2788047247902625 1105 98-99 0.033105378657747764 1105 100-101 -0.3092735064899834 1106 1 0.15026746617024145 1106 2 0.1848545974363489 1106 3 0.31488027778689087 1106 4 0.2328397738593182 1106 5 0.19258836916486644 1106 6 0.19389268929717218 1106 7 0.23930893391876396 1106 8 0.24168386282477172 1106 9 0.24036535531587333 1106 10-11 0.3700495817137366 1106 12-13 0.32676868424041317 1106 14-15 0.4013667798243219 1106 16-17 0.5003125149453425 1106 18-19 0.51673301914159 1106 20-21 0.4131759505915156 1106 22-23 0.47863098781110125 1106 24-25 0.5009938021299405 1106 26-27 0.40794288249822586 1106 28-29 0.4622877239434331 1106 30-31 0.41555517568161093 1106 32-33 0.4983680209902204 1106 34-35 0.5242945599293733 1106 36-37 0.43342014449607547 1106 38-39 0.4931004779083281 1106 40-41 0.5678786053829086 1106 42-43 0.6890949402075179 1106 44-45 0.5202993651817494 1106 46-47 0.6596652457377914 1106 48-49 0.6669715106518126 1106 50-51 0.750409044291878 1106 52-53 0.7924086328110818 1106 54-55 0.6702498359573354 1106 56-57 0.7132066587946895 1106 58-59 0.6878127104712561 1106 60-61 0.8338684369607279 1106 62-63 0.695243276720312 1106 64-65 0.6598255416045973 1106 66-67 0.6617354259577368 1106 68-69 0.7160237866475896 1106 70-71 0.6553786957167986 1106 72-73 0.7069729397818847 1106 74-75 0.777599116532766 1106 76-77 0.733950725358202 1106 78-79 0.7202179259262671 1106 80-81 0.6404041738834252 1106 82-83 0.6516507418183473 1106 84-85 0.6081505578062121 1106 86-87 0.5133944529208456 1106 88-89 0.5317192934993926 1106 90-91 0.6266369821190096 1106 92-93 0.5897319781809252 1106 94-95 0.6355914132641161 1106 96-97 0.6663206699581536 1106 98-99 0.6311986384602548 1106 100-101 0.3390967785254766 1107 1 0.09256115838808654 1107 2 0.198950413429678 1107 3 0.24249095078105398 1107 4 0.18146879634225144 1107 5 0.17755885595830279 1107 6 0.18075138464325846 1107 7 0.17607463909523347 1107 8 0.24105650057450134 1107 9 0.17828832590796395 1107 10-11 0.19836009909170116 1107 12-13 0.19719536158350337 1107 14-15 0.27621719359691355 1107 16-17 0.1829883055454431 1107 18-19 0.2441470598613691 1107 20-21 0.2759584564408968 1107 22-23 0.32930248825248043 1107 24-25 0.2885819713806228 1107 26-27 0.2316494376011704 1107 28-29 0.2026257453526057 1107 30-31 0.18519018161363476 1107 32-33 0.15580252077167955 1107 34-35 0.15120955136497827 1107 36-37 0.1598179661141188 1107 38-39 0.14061900554152373 1107 40-41 0.19290367416135012 1107 42-43 0.1692928703038845 1107 44-45 0.17536717638048316 1107 46-47 0.22942146334580826 1107 48-49 0.1729728354097233 1107 50-51 0.11909921133136692 1107 52-53 0.27511050228692113 1107 54-55 0.17732835296308735 1107 56-57 0.13640094152152926 1107 58-59 0.12135247096187385 1107 60-61 -5.294777087030411E-4 1107 62-63 0.08141143282136198 1107 64-65 0.07358416875381835 1107 66-67 0.14475171117493346 1107 68-69 0.1154147898027631 1107 70-71 0.098581353068532 1107 72-73 0.17284732285730797 1107 74-75 0.1503220817376807 1107 76-77 0.21089687676505164 1107 78-79 0.2327867196741913 1107 80-81 0.09171638210304423 1107 82-83 0.09682939436079252 1107 84-85 0.1211994232526834 1107 86-87 0.15079131586472627 1107 88-89 0.09921509700424735 1107 90-91 0.1955962635664008 1107 92-93 0.16364771525198663 1107 94-95 0.10991633156867309 1107 96-97 0.17442862915757118 1107 98-99 0.10945681443266864 1107 100-101 0.04825499404473277 1108 1 -0.020525150945282178 1108 2 0.15588487976371823 1108 3 0.12772752398358023 1108 4 0.10030108058190024 1108 5 0.18163323846717105 1108 6 0.23305487702846506 1108 7 0.10596130675515525 1108 8 0.21090876170804052 1108 9 0.2217080305152308 1108 10-11 0.2662445156411124 1108 12-13 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-1.0233720552634011 2316 62-63 -1.0572280552909135 2316 64-65 -1.269141443406177 2316 66-67 -1.2576529305661666 2316 68-69 -1.2442475134754858 2316 70-71 -1.1918413020976075 2316 72-73 -1.515993160243017 2316 74-75 -1.2151634367410473 2316 76-77 -1.1276678493129282 2316 78-79 -0.9553206345564291 2316 80-81 -0.9889919576175927 2316 82-83 -1.0128527255706388 2316 84-85 -1.1279352786577164 2316 86-87 -1.2175235318194169 2316 88-89 -1.2716937243920121 2316 90-91 -1.4440062091328834 2316 92-93 -1.402488924325521 2316 94-95 -1.2970952891961112 2316 96-97 -1.3629695857024373 2316 98-99 -1.4919485791190787 2316 100-101 -1.397783037168157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6735.0 3 1765.0 4 1751.0 5 1941.0 6 2313.0 7 2705.0 8 3192.0 9 4043.0 10 4939.0 11 6072.0 12 8261.0 13 13116.0 14 11176.0 15 11574.0 16 14409.0 17 21116.0 18 17593.0 19 11899.0 20 11553.0 21 12119.0 22 12641.0 23 13999.0 24 15453.0 25 17498.0 26 20215.0 27 23560.0 28 28270.0 29 33228.0 30 40344.0 31 49249.0 32 62294.0 33 82064.0 34 114720.0 35 172967.0 36 283490.0 37 469891.0 38 458048.0 39 79371.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.930525759231145 18.584406195971823 14.435744047688676 23.04932399710836 2 15.39537622637882 19.86227414705254 40.751612852048765 23.990736774519878 3 19.491671737288293 24.305620364114183 29.535002221118923 26.667705677478597 4 13.023819079530712 16.299875864514984 36.52285306278642 34.153451993167884 5 14.128694860027235 37.050244216142254 33.610357415201044 15.21070350862947 6 32.30987149164927 35.11160819434876 17.484047810162295 15.094472503839674 7 29.023339818559812 32.69109222813009 20.23847783913684 18.04709011417326 8 26.45494896274308 34.74605443103393 19.579052242523524 19.219944363699458 9 26.834527642627958 17.158733290514512 20.4707085208989 35.53603054595864 10-11 25.48128599777189 26.318518057276467 26.625728584291895 21.57446736065975 12-13 26.35075190775603 23.743026661940032 27.473109502397524 22.433111927906413 14-15 23.181498476225478 24.9400191602869 26.08611494866181 25.792367414825808 16-17 22.542146508397117 28.38604566881353 26.53672761330041 22.535080209488946 18-19 22.334054800399294 26.6238373340548 29.25612702795996 21.785980837585946 20-21 23.199294901187745 25.82242481037292 29.111890822567084 21.866389465872256 22-23 22.819695058787964 25.75164087780636 29.029151282647575 22.399512780758105 24-25 22.728012873540052 26.025201195720083 29.075948380799616 22.170837549940256 26-27 22.4267388671259 26.20333580305437 29.102800738303507 22.26712459151623 28-29 22.089954927361717 26.462528033352168 29.243562534611595 22.20395450467452 30-31 22.27746789714959 26.09015808032722 29.102883530340907 22.529490492182287 32-33 22.143898910214382 26.231708719381196 29.00747476825466 22.616917602149762 34-35 22.864555200916605 26.309260753159595 28.641506786854197 22.1846772590696 36-37 22.790808709062794 25.9657037956256 28.75015901299725 22.493328482314357 38-39 22.45605569967149 26.254454744016915 28.869879899047273 22.41960965726432 40-41 22.648436256006292 26.16526792990081 28.821267667637663 22.36502814645523 42-43 22.469446489400053 26.304232086052252 28.435816254277913 22.790505170269785 44-45 22.603605019676117 26.47822696392802 28.355674473005926 22.56249354338994 46-47 22.823777601729294 26.71293170854429 27.759235947497157 22.704054742229257 48-49 22.965169873033002 26.68926033798677 27.655000401757235 22.690569387222993 50-51 22.645398133752064 27.032718255032645 27.635677555572684 22.686206055642607 52-53 23.030115057341266 26.925399664732335 27.396067492061054 22.648417785865345 54-55 22.546552002404287 26.73840483686859 27.685244678959663 23.02979848176746 56-57 22.748606402819135 27.153155651058814 27.377194371094486 22.721043575027565 58-59 22.902760290942435 26.564173843326333 27.59988882314333 22.9331770425879 60-61 22.774554758830575 26.611097966280116 28.01358512432631 22.600762150563 62-63 22.705507036061764 26.746965264341526 27.90066719801426 22.64686050158245 64-65 22.440203515844445 26.68181931057813 27.756373223582088 23.121603949995336 66-67 22.422561030040747 26.89098646400989 27.546640744143243 23.139811761806122 68-69 22.493776724116316 26.69336022216562 27.79047988176537 23.022383171952693 70-71 22.506377022006877 26.79366192199671 27.616312991691476 23.08364806430494 72-73 22.715826604920366 26.537140324135272 27.651516393961135 23.095516676983227 74-75 22.517218531253896 26.95493238639612 27.4636627838867 23.064186298463287 76-77 22.630612397871225 26.796388951352974 27.4282756165205 23.144723034255303 78-79 22.60482519532805 26.821836275704573 27.501187283560785 23.072151245406594 80-81 22.532933294519168 27.05716431623176 27.42044989612215 22.98945249312692 82-83 22.676426849340608 26.830766218218876 27.347546529083445 23.145260403357067 84-85 22.62891051616202 26.69797164096291 27.354682829270782 23.31843501360429 86-87 22.705944021057096 26.66979581378202 27.476792549257134 23.14746761590375 88-89 22.569343510234486 26.635872054006132 27.287922991606045 23.506861444153337 90-91 22.626075010097622 26.69640037372913 27.4263342214242 23.25119039474905 92-93 22.723665611625275 26.467153021300682 27.494922188647397 23.314259178426642 94-95 22.634397290898107 26.364402693545262 27.517514088819894 23.48368592673673 96-97 22.70474051082509 26.29053434771118 27.498739524936788 23.505985616526935 98-99 22.539421918080382 26.33126875504899 27.167971202741025 23.961338124129604 100-101 22.92032315875006 25.63073976051436 27.795578268977124 23.65335881175846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1953.0 1 1095.5 2 312.5 3 394.5 4 362.0 5 372.5 6 634.5 7 1327.0 8 2143.5 9 2670.5 10 2704.0 11 2564.5 12 2704.0 13 3005.0 14 3340.5 15 3600.5 16 3776.0 17 3930.0 18 4113.5 19 4208.5 20 4436.5 21 4950.0 22 5641.5 23 6693.0 24 7893.5 25 9400.5 26 11639.5 27 14467.5 28 16990.5 29 19689.5 30 22714.5 31 26414.5 32 31219.5 33 34013.0 34 36133.5 35 39050.0 36 42620.0 37 47194.5 38 50223.5 39 51920.0 40 54245.5 41 59512.5 42 64764.5 43 69079.0 44 73535.0 45 76751.0 46 77173.0 47 78666.0 48 89114.5 49 92420.0 50 86557.0 51 83518.0 52 80116.0 53 79259.0 54 77835.5 55 71533.0 56 64350.0 57 57917.0 58 51668.0 59 45232.5 60 38161.5 61 31239.5 62 26007.5 63 21000.5 64 16193.0 65 12510.5 66 9266.0 67 7409.5 68 5703.0 69 3968.0 70 3011.5 71 2277.5 72 1846.5 73 1542.5 74 1278.5 75 1115.0 76 779.5 77 474.5 78 322.5 79 189.0 80 119.5 81 98.5 82 70.0 83 45.0 84 36.5 85 29.5 86 28.0 87 23.0 88 13.5 89 7.0 90 8.0 91 7.5 92 5.5 93 6.0 94 4.5 95 3.5 96 4.0 97 3.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39163906752336647 2 0.11651763284641391 3 0.11810227265312515 4 0.12947439361893515 5 0.13161831806330918 6 0.07154182656769814 7 0.0816089500456283 8 0.07802020695395875 9 0.07834645632592872 10-11 0.07589958603615402 12-13 0.08007091729205564 14-15 0.07363914395893359 16-17 0.07543351550476836 18-19 0.0847316226059122 20-21 0.08319358985233953 22-23 0.09342383801625467 24-25 0.10659033052789946 26-27 0.10747586453753219 28-29 0.10109069825754871 30-31 0.09046429014195576 32-33 0.08946223849947661 34-35 0.3389730974767874 36-37 0.5284540720116256 38-39 0.5096714295967838 40-41 0.48182371534649077 42-43 0.5099510719156151 44-45 0.5200181953935453 46-47 0.5166391840409993 48-49 0.5231408679538292 50-51 0.5223951551036121 52-53 0.5212532823017173 54-55 0.5162430240893215 56-57 0.4882554886796129 58-59 0.4782582757813906 60-61 0.42615159037247424 62-63 0.5021676940414747 64-65 0.5274520203691465 66-67 0.5381483390644473 68-69 0.5534354524938968 70-71 0.5483319801752239 72-73 0.5354684335089798 74-75 0.538777534281818 76-77 0.5144486525434867 78-79 0.4880690604670586 80-81 0.49582913481462976 82-83 0.5098112507561994 84-85 0.5030532280511074 86-87 0.5048009925438036 88-89 0.5138660643792546 90-91 0.5328118314800815 92-93 0.5387076237021101 94-95 0.5340702219148228 96-97 0.5351421841370098 98-99 0.5362607534123354 100-101 0.5118153540411577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2145598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.748341768726114 #Duplication Level Percentage of deduplicated Percentage of total 1 75.35777341413281 39.749975869789004 2 13.571903812088268 14.317908414646174 3 4.767420759817631 7.544206187825411 4 2.1298027807445554 4.4937425991478825 5 1.1578652014629505 3.0537734684441316 6 0.6871392343555369 2.1747273105891995 7 0.46130884806238087 1.7033293744972269 8 0.3116046509451899 1.3149302899825186 9 0.21444287892462907 1.01803556406473 >10 1.1661098618722383 11.329225060004386 >50 0.1026792027332605 3.774137683126809 >100 0.06644450234167155 6.530213782962692 >500 0.004439397192593795 1.5452933755492348 >1k 8.878794385187488E-4 0.7639121154291058 >5k 1.7757588770374975E-4 0.6865889039415543 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7984 0.3721107122583075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6676 0.31114868675306373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4055 0.18899160047688338 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2534 0.11810227265312515 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.6607053138565565E-5 9 0.0 0.0 0.0 4.6607053138565565E-5 4.6607053138565565E-5 10-11 0.0 0.0 0.0 4.6607053138565565E-5 4.6607053138565565E-5 12-13 0.0 0.0 0.0 9.321410627713113E-5 4.6607053138565565E-5 14-15 0.0 0.0 0.0 1.398211594156967E-4 6.991057970784835E-5 16-17 0.0 0.0 0.0 2.0973173912354503E-4 1.398211594156967E-4 18-19 0.0 0.0 0.0 2.563387922621106E-4 1.398211594156967E-4 20-21 0.0 0.0 0.0 2.796423188313934E-4 1.398211594156967E-4 22-23 0.0 0.0 0.0 3.4955289853924175E-4 1.398211594156967E-4 24-25 0.0 0.0 0.0 5.35981111093504E-4 1.398211594156967E-4 26-27 0.0 0.0 0.0 8.855340096327458E-4 1.398211594156967E-4 28-29 0.0 0.0 0.0 0.0029595478742989135 1.398211594156967E-4 30-31 0.0 0.0 0.0 0.009298107101143831 1.398211594156967E-4 32-33 0.0 0.0 0.0 0.02011094342929104 1.8642821255426226E-4 34-35 0.0 0.0 0.0 0.03570100270414123 1.8642821255426226E-4 36-37 0.0 0.0 0.0 0.06250005825881642 1.8642821255426226E-4 38-39 0.0 0.0 0.0 0.1043531919772483 1.8642821255426226E-4 40-41 0.0 0.0 0.0 0.15165935091289234 2.0973173912354503E-4 42-43 0.0 0.0 0.0 0.20038702496926264 2.3303526569282783E-4 44-45 0.0 0.0 0.0 0.26085967641655144 2.3303526569282783E-4 46-47 0.0 0.0 0.0 0.3375748858826304 2.3303526569282783E-4 48-49 0.0 0.0 0.0 0.4215840991648948 2.3303526569282783E-4 50-51 0.0 0.0 0.0 0.5124678527850977 2.3303526569282783E-4 52-53 0.0 0.0 0.0 0.6068238318641237 2.796423188313934E-4 54-55 0.0 0.0 0.0 0.7099652404597693 3.26249371969959E-4 56-57 0.0 0.0 0.0 0.8353615169290799 3.26249371969959E-4 58-59 0.0 0.0 0.0 0.9783053489050606 3.26249371969959E-4 60-61 0.0 0.0 0.0 1.1399153056630364 3.26249371969959E-4 62-63 0.0 0.0 0.0 1.3017116906335668 3.26249371969959E-4 64-65 0.0 0.0 0.0 1.4687280655556165 3.26249371969959E-4 66-67 0.0 0.0 0.0 1.6669711660805053 3.26249371969959E-4 68-69 0.0 0.0 0.0 1.904037941869819 3.26249371969959E-4 70-71 0.0 0.0 0.0 2.1600504847599598 3.26249371969959E-4 72-73 0.0 0.0 0.0 2.4205839118045414 3.26249371969959E-4 74-75 0.0 0.0 0.0 2.692862316240041 3.26249371969959E-4 76-77 0.0 0.0 0.0 2.9886306754573786 3.26249371969959E-4 78-79 0.0 0.0 0.0 3.3285825210500755 3.26249371969959E-4 80-81 0.0 0.0 0.0 3.7084766111825234 3.4955289853924175E-4 82-83 0.0 0.0 0.0 4.101443979720339 3.728564251085245E-4 84-85 0.0 0.0 0.0 4.501099460383539 3.728564251085245E-4 86-87 0.0 0.0 0.0 4.921471776166831 3.728564251085245E-4 88-89 0.0 0.0 0.0 5.385911060692637 3.728564251085245E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8440 0.0 31.224121 1 GTATCAA 12255 0.0 29.911879 1 GTACATG 10240 0.0 24.947212 1 TATCAAC 14825 0.0 24.827385 2 TACATGG 10125 0.0 24.702652 2 TCAACGC 14985 0.0 24.435848 4 CAACGCA 15025 0.0 24.370794 5 ATCAACG 15170 0.0 24.106623 3 AACGCAG 15305 0.0 23.971178 6 ACATGGG 10220 0.0 23.221567 3 GAGTACT 8000 0.0 22.606842 12-13 ACGCAGA 17005 0.0 21.46341 7 GTACTTT 8445 0.0 21.360287 14-15 CGCAGAG 17320 0.0 20.963724 8 AGTACTT 8225 0.0 20.347921 12-13 GCAGAGT 17955 0.0 19.774103 9 ATAACGC 170 8.127096E-5 19.505354 3 TACCTGG 1725 0.0 19.222666 2 CATGGGG 4965 0.0 19.177036 4 AGAGTAC 14690 0.0 18.997576 10-11 >>END_MODULE