FastQCFastQC Report
Fri 27 May 2016
SRR939210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145598
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.36856857621977646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.3267620495544832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.20064336376152478No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28030.13063956994739928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133600.028.3260121
GGTATCA96250.027.5324881
TATCAAC159350.023.6463132
TCAACGC161800.023.2882584
ATCAACG162000.023.2302113
CAACGCA162450.023.1658635
GTACATG102600.022.7625081
AACGCAG165900.022.7413226
GAGTACT80500.021.8732612-13
TACATGG104200.021.7698352
AGTACTT82000.021.12586612-13
ACATGGG104100.020.7422393
GTACTTT85400.020.5626614-15
ACGCAGA184150.020.2814047
CGCAGAG185850.020.0448158
GCAGAGT194350.018.9732449
AGAGTAC152750.018.75130710-11
TACTTTT94000.018.45419114-15
TAACGCA1300.00760535618.252474
GTATAGA6300.018.1569161
Produced by FastQC (version 0.11.5)