##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2145598 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.494724547655245 34.0 31.0 34.0 31.0 34.0 2 32.71402844335239 34.0 31.0 34.0 31.0 34.0 3 32.815228668184815 34.0 31.0 34.0 31.0 34.0 4 36.26817139091293 37.0 37.0 37.0 35.0 37.0 5 36.23024676570355 37.0 37.0 37.0 35.0 37.0 6 36.27107128175921 37.0 37.0 37.0 35.0 37.0 7 36.22678479379641 37.0 37.0 37.0 35.0 37.0 8 36.20330649077786 37.0 37.0 37.0 35.0 37.0 9 37.94563007609067 39.0 38.0 39.0 35.0 39.0 10-11 37.86223188127506 39.0 38.0 39.0 35.0 39.0 12-13 37.77188550697754 39.0 38.0 39.0 35.0 39.0 14-15 39.18487899410793 40.5 38.5 41.0 36.0 41.0 16-17 39.28134347627095 41.0 39.0 41.0 36.0 41.0 18-19 39.316068760317634 41.0 39.0 41.0 36.0 41.0 20-21 39.28826508973256 41.0 39.0 41.0 36.0 41.0 22-23 39.218955974045464 41.0 39.0 41.0 36.0 41.0 24-25 39.14026252820892 41.0 39.0 41.0 35.5 41.0 26-27 38.99826505244692 40.0 39.0 41.0 35.0 41.0 28-29 38.838090825960876 40.0 38.0 41.0 35.0 41.0 30-31 38.64075632993692 40.0 38.0 41.0 35.0 41.0 32-33 38.541343485592364 40.0 38.0 41.0 34.0 41.0 34-35 38.42743491558065 40.0 38.0 41.0 34.0 41.0 36-37 38.27094101504569 40.0 38.0 41.0 34.0 41.0 38-39 38.137004229124 40.0 38.0 41.0 34.0 41.0 40-41 37.94168502207776 40.0 38.0 41.0 33.0 41.0 42-43 37.73636557267484 40.0 37.0 41.0 33.0 41.0 44-45 37.448684236282844 40.0 37.0 41.0 33.0 41.0 46-47 37.09525968983938 40.0 37.0 41.0 31.5 41.0 48-49 36.711022521460215 40.0 36.0 41.0 31.0 41.0 50-51 36.42074913380792 39.5 36.0 41.0 30.0 41.0 52-53 36.13769424654572 39.0 35.0 41.0 30.0 41.0 54-55 35.867984356808684 39.0 35.0 40.0 29.0 41.0 56-57 35.60902065531381 38.5 35.0 40.0 28.5 41.0 58-59 35.2557349978887 38.0 35.0 40.0 28.0 41.0 60-61 34.86778068398647 38.0 34.0 40.0 27.0 41.0 62-63 34.45439593064498 37.0 34.0 40.0 26.0 41.0 64-65 33.9815349846523 36.5 33.5 39.5 26.0 41.0 66-67 33.54236464612663 36.0 33.0 39.0 25.0 40.5 68-69 33.04106780487305 35.5 32.5 39.0 23.5 40.0 70-71 32.51789384591149 35.0 32.0 38.0 22.0 40.0 72-73 31.935424995735453 35.0 31.5 37.0 20.5 39.0 74-75 31.381505528994715 35.0 31.0 36.5 18.5 39.0 76-77 29.398803736767093 32.5 28.5 35.0 14.5 36.5 78-79 30.392227481569243 34.0 30.0 35.0 16.5 37.0 80-81 30.400764961563162 34.0 30.0 35.0 14.0 37.0 82-83 30.018183974817276 34.0 30.0 35.0 8.5 37.0 84-85 29.544167639977292 34.0 29.5 35.0 4.5 36.0 86-87 28.82478101676083 34.0 29.0 35.0 2.0 36.0 88-89 28.13600334265785 33.0 27.5 35.0 2.0 35.0 90-91 27.55568331066677 33.0 26.5 35.0 2.0 35.0 92-93 27.089706692493188 33.0 26.0 34.5 2.0 35.0 94-95 26.522288424951924 32.0 25.0 34.0 2.0 35.0 96-97 25.687827822360013 32.0 23.5 34.0 2.0 35.0 98-99 24.430250913731278 32.0 10.5 34.0 2.0 35.0 100-101 18.58622351437688 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.044307873745964343 1101 2 0.053835539835574764 1101 3 0.08725997229440452 1101 4 0.08832183634601876 1101 5 0.074200385192988 1101 6 0.05958438585255976 1101 7 0.16162286892523525 1101 8 0.15869522676992887 1101 9 0.22770730977708098 1101 10-11 0.12946464280292957 1101 12-13 0.1491830971994048 1101 14-15 0.2748498291679766 1101 16-17 0.2265105276269921 1101 18-19 0.24252109679240874 1101 20-21 0.22881993221030683 1101 22-23 0.16450062543265176 1101 24-25 0.1296763345034364 1101 26-27 0.12030478575324821 1101 28-29 0.1273735002812515 1101 30-31 0.10206983843760753 1101 32-33 0.22501055882851517 1101 34-35 0.1763660420664408 1101 36-37 0.11142303261262754 1101 38-39 0.17003036690378082 1101 40-41 0.21489259403291783 1101 42-43 0.10075588145559067 1101 44-45 0.1367292411730432 1101 46-47 0.20468112394789273 1101 48-49 0.11976981856245317 1101 50-51 0.14838084507809413 1101 52-53 0.12907253386519812 1101 54-55 0.060592479053227066 1101 56-57 0.0756860554549803 1101 58-59 0.02687006521958324 1101 60-61 -0.002131424407672 1101 62-63 -0.12325195175118608 1101 64-65 -0.04914602164146231 1101 66-67 -0.12040053824542696 1101 68-69 -0.06650723290484706 1101 70-71 -0.27501667399766205 1101 72-73 -0.27834148688647176 1101 74-75 -0.39507618495499486 1101 76-77 -0.15031798763383364 1101 78-79 -0.08798119031555984 1101 80-81 -0.17050793258670183 1101 82-83 -0.24016219501015357 1101 84-85 -0.20821281577844886 1101 86-87 0.0533958586908625 1101 88-89 0.034422453502827466 1101 90-91 -0.011285126026653103 1101 92-93 -0.20332241135804452 1101 94-95 -0.3040020306890021 1101 96-97 -0.060954209407384496 1101 98-99 -0.2516870056687104 1101 100-101 -0.5117712275178228 1102 1 -0.02556835840380245 1102 2 -0.023208210999570156 1102 3 -0.005116030828531848 1102 4 0.06134305911006521 1102 5 0.11470312388708948 1102 6 0.04976482398526372 1102 7 0.1210889125611132 1102 8 0.09208430455328198 1102 9 0.0806755850387404 1102 10-11 0.08955255993080868 1102 12-13 0.1306625580148122 1102 14-15 0.09467310798027029 1102 16-17 0.17469896915337557 1102 18-19 0.1224472888843593 1102 20-21 0.19052795888595142 1102 22-23 0.059215094618473074 1102 24-25 0.11600182801979031 1102 26-27 0.07608918660447017 1102 28-29 0.02798314928377721 1102 30-31 -0.021061550608671098 1102 32-33 0.05983451159238484 1102 34-35 0.027504582584931825 1102 36-37 0.04714730881490681 1102 38-39 0.0341874052046478 1102 40-41 0.07984991335117542 1102 42-43 -0.05708824375818722 1102 44-45 0.11406419160113757 1102 46-47 -0.020332778126650908 1102 48-49 0.011062160080264505 1102 50-51 -0.10688020758173877 1102 52-53 0.05256663139152806 1102 54-55 -0.1514611301470552 1102 56-57 -0.013857454683176229 1102 58-59 -0.21597831668397305 1102 60-61 -0.4468898609100549 1102 62-63 -0.3954698884324088 1102 64-65 -0.16147449221487165 1102 66-67 -0.15153797593408314 1102 68-69 -0.1397704363746115 1102 70-71 -0.2701955862573371 1102 72-73 -0.3453281881590442 1102 74-75 -0.27492491637974936 1102 76-77 -0.31935831561192884 1102 78-79 -0.19336197878637762 1102 80-81 -0.27250233324536666 1102 82-83 -0.23186690152612854 1102 84-85 -0.34430799874608553 1102 86-87 -0.030731093945220778 1102 88-89 -0.14195213052901323 1102 90-91 -0.08723086096354749 1102 92-93 -0.20157013728207218 1102 94-95 -0.1860590475751316 1102 96-97 -0.4449826075674679 1102 98-99 -0.35461078845674976 1102 100-101 -0.6066608365850676 1103 1 0.03359588194186358 1103 2 0.08368691552026064 1103 3 0.08410983749505618 1103 4 0.039670802982669784 1103 5 0.11474399430024818 1103 6 0.02422484523788171 1103 7 0.09583099722849653 1103 8 0.004245727718632963 1103 9 0.0601004980308133 1103 10-11 0.043345967475602265 1103 12-13 0.06291092578866397 1103 14-15 -0.11189689728107055 1103 16-17 0.07003358270923599 1103 18-19 0.10824136647367055 1103 20-21 0.16734349747164856 1103 22-23 0.15146422223663336 1103 24-25 0.032431331162207755 1103 26-27 -0.03181676309153403 1103 28-29 0.07245994467497496 1103 30-31 0.2104696277601832 1103 32-33 0.13345478976447822 1103 34-35 0.15246385608168112 1103 36-37 0.08323991311217327 1103 38-39 0.11454381823298831 1103 40-41 0.21058659391378143 1103 42-43 0.10558031791411082 1103 44-45 0.19819564927032474 1103 46-47 0.3275757504348533 1103 48-49 0.22829847971682682 1103 50-51 0.11968636686745526 1103 52-53 0.1760364373664487 1103 54-55 0.15088120503868652 1103 56-57 0.17010516788489127 1103 58-59 0.13834139816718505 1103 60-61 0.18785499834029906 1103 62-63 0.09032936991349771 1103 64-65 0.03367683768534846 1103 66-67 -0.014728585179547338 1103 68-69 0.10681314246901508 1103 70-71 0.20752399435465918 1103 72-73 0.09843153295507179 1103 74-75 -0.12752749823858167 1103 76-77 -0.13284273132044078 1103 78-79 0.0170219267903029 1103 80-81 -0.05910840808542517 1103 82-83 -0.07162408522079033 1103 84-85 -0.4265222886543185 1103 86-87 -0.26518076519743516 1103 88-89 -0.06593891844840982 1103 90-91 0.07356975013547995 1103 92-93 0.19446128803616958 1103 94-95 -0.07625866788324132 1103 96-97 -0.3173575469813983 1103 98-99 -0.5379986348622801 1103 100-101 -1.1959744899510056 1104 1 0.13369231298356965 1104 2 0.09444663393488639 1104 3 0.13100426990666136 1104 4 0.06409495146964161 1104 5 0.1244089763687839 1104 6 0.07093499843364981 1104 7 0.13067239088424287 1104 8 0.12065381446281265 1104 9 0.18099187090255242 1104 10-11 0.14214445015925037 1104 12-13 0.1701572221130121 1104 14-15 0.0737437969551138 1104 16-17 0.10077623636360045 1104 18-19 0.18903728439453715 1104 20-21 0.2562901944981064 1104 22-23 0.22365960196120938 1104 24-25 0.17860051126275067 1104 26-27 0.1724184613336206 1104 28-29 0.1939857765820392 1104 30-31 0.1397501646774444 1104 32-33 0.20486551027097022 1104 34-35 0.2530883017714771 1104 36-37 0.27988603138408763 1104 38-39 0.25428468294848017 1104 40-41 0.22848734230618106 1104 42-43 0.2546567340451915 1104 44-45 0.35361160700895056 1104 46-47 0.376986561818768 1104 48-49 0.3283385330988935 1104 50-51 0.23176599568368061 1104 52-53 0.11896709957675 1104 54-55 0.09510434613261509 1104 56-57 0.035394536741200966 1104 58-59 0.039897169135883814 1104 60-61 0.06126064654034025 1104 62-63 -0.03526238514829316 1104 64-65 -0.06860443803169858 1104 66-67 -0.06322767724679323 1104 68-69 0.05927709863244246 1104 70-71 0.03936205752119548 1104 72-73 -0.10714034687805096 1104 74-75 -0.03015023745235723 1104 76-77 0.10230848702602913 1104 78-79 0.1648534063925311 1104 80-81 0.2596483551300892 1104 82-83 0.3073187747589792 1104 84-85 0.08370394563777239 1104 86-87 0.1516743879730953 1104 88-89 0.17849778458605314 1104 90-91 0.29392898521685495 1104 92-93 0.07942646010583232 1104 94-95 0.09397409611987584 1104 96-97 -0.29844662873237127 1104 98-99 -0.35583147762583067 1104 100-101 -0.8415727410655158 1105 1 -0.016500409230701507 1105 2 -0.04555917132038445 1105 3 0.01936611909103192 1105 4 0.019694877158109136 1105 5 0.022831402245827803 1105 6 -0.007948238905072458 1105 7 0.048504839702438574 1105 8 -0.0020556351760632197 1105 9 0.12768374165273144 1105 10-11 0.09138138427013587 1105 12-13 0.04209483667550984 1105 14-15 0.07690070436928664 1105 16-17 0.06994041553968344 1105 18-19 0.08847776226992465 1105 20-21 0.08260298047956383 1105 22-23 0.06679323504614842 1105 24-25 0.05044203792227364 1105 26-27 0.19834540307557091 1105 28-29 0.14601445742174235 1105 30-31 0.0944579788229234 1105 32-33 0.17562426886323834 1105 34-35 0.17345701810906178 1105 36-37 0.2664577136260533 1105 38-39 0.2590331045614036 1105 40-41 0.2093166728653557 1105 42-43 0.1549891901932554 1105 44-45 0.049419429070688636 1105 46-47 0.08382596814745824 1105 48-49 0.21186373571642747 1105 50-51 0.148548007815009 1105 52-53 0.09229491018788849 1105 54-55 -0.09247310058391633 1105 56-57 -0.03660568821910459 1105 58-59 0.03444521348718865 1105 60-61 -0.25563938978915246 1105 62-63 -0.32774130111682354 1105 64-65 -0.07595203075652535 1105 66-67 0.027200235102917247 1105 68-69 -0.1686601992797705 1105 70-71 -0.14589501959940776 1105 72-73 -0.39227206993272645 1105 74-75 -0.32806844838889404 1105 76-77 -0.3923232510881931 1105 78-79 -0.42991978484265303 1105 80-81 -0.2858593288369313 1105 82-83 -0.332262697787467 1105 84-85 -0.3707240008291386 1105 86-87 -0.26947875748640016 1105 88-89 -0.32779257466542333 1105 90-91 -0.30835012388702765 1105 92-93 -0.4084295482400826 1105 94-95 -0.3008689554186894 1105 96-97 -0.6961230073405638 1105 98-99 -0.5158336952440088 1105 100-101 -1.1238382192807492 1106 1 0.1004328773429819 1106 2 0.09417997665205746 1106 3 0.04733018610119899 1106 4 0.06973463570363236 1106 5 0.12382170475761711 1106 6 0.1348647135169898 1106 7 0.09482849096283275 1106 8 0.10903115406767228 1106 9 0.17984078726522768 1106 10-11 0.15999641235804063 1106 12-13 0.15108848943889086 1106 14-15 0.1756674829251068 1106 16-17 0.051553726109808906 1106 18-19 0.05706812592894295 1106 20-21 0.17744683023722274 1106 22-23 0.18955053835053803 1106 24-25 0.12545555839696476 1106 26-27 0.1852363875845029 1106 28-29 0.22290401601838283 1106 30-31 0.23047189880997365 1106 32-33 0.3071645496009623 1106 34-35 0.26282754848420353 1106 36-37 0.2783052463481752 1106 38-39 0.34116295507184446 1106 40-41 0.22753957507217848 1106 42-43 0.27788446014671564 1106 44-45 0.4145768125344702 1106 46-47 0.30210552146437664 1106 48-49 0.1848468084675048 1106 50-51 0.22912014548876414 1106 52-53 0.21462238443476167 1106 54-55 0.2633216913677785 1106 56-57 0.18236883676583204 1106 58-59 0.1456643171150418 1106 60-61 0.32184815274735 1106 62-63 0.37212933909210477 1106 64-65 0.31986439395465993 1106 66-67 0.2818956615213466 1106 68-69 0.020415514093876652 1106 70-71 -0.11075308994184496 1106 72-73 -0.06136561363308246 1106 74-75 -0.2860966557602218 1106 76-77 -0.1965083679038102 1106 78-79 -0.22470942424099505 1106 80-81 -0.05693874444412117 1106 82-83 -0.07140103568757183 1106 84-85 -0.15543680796229964 1106 86-87 -0.07024155633385121 1106 88-89 0.11563313466171365 1106 90-91 0.16726677587133665 1106 92-93 -0.015312336964843354 1106 94-95 -0.2240780545375749 1106 96-97 -0.5522668266530673 1106 98-99 -0.5163368752853437 1106 100-101 -0.8608064728208014 1107 1 0.03607168124085547 1107 2 0.032147024217231035 1107 3 0.023670401564139354 1107 4 0.027151627131878797 1107 5 -0.04465468896238889 1107 6 0.0358357537213152 1107 7 0.023367229112551513 1107 8 0.1193564298854568 1107 9 0.1303163900484421 1107 10-11 0.11459884418259492 1107 12-13 0.04953881652676273 1107 14-15 0.053849381246877215 1107 16-17 0.07394384894988093 1107 18-19 0.12501045365838337 1107 20-21 0.11107584659835368 1107 22-23 0.11580596685830358 1107 24-25 0.06197320816378493 1107 26-27 0.07765172000446796 1107 28-29 0.15986941437014934 1107 30-31 0.09576128250369464 1107 32-33 0.1206279834665267 1107 34-35 5.740867450043652E-5 1107 36-37 0.11782600721467418 1107 38-39 0.03812788816667734 1107 40-41 0.126180045324503 1107 42-43 0.09491127241432196 1107 44-45 0.045610552233007695 1107 46-47 0.058321905120145345 1107 48-49 -0.024827230072908435 1107 50-51 -0.11201983195851994 1107 52-53 -0.05572483192073463 1107 54-55 -0.325188176364577 1107 56-57 -0.2716503097815348 1107 58-59 -0.25515061714293097 1107 60-61 -0.2539693832763845 1107 62-63 -0.2175442831657861 1107 64-65 -0.21483863131736314 1107 66-67 -0.22250811783422364 1107 68-69 -0.36007356889694364 1107 70-71 -0.3277977778707495 1107 72-73 -0.4712656690966064 1107 74-75 -0.4959811618471299 1107 76-77 -0.5169128231063027 1107 78-79 -0.2184585748409944 1107 80-81 -0.1489530988450234 1107 82-83 -0.2619133447944897 1107 84-85 -0.28074972386220765 1107 86-87 -0.3040980588505242 1107 88-89 -0.3850061038840913 1107 90-91 -0.17340598566121912 1107 92-93 0.054196559849739145 1107 94-95 -0.10136421081738334 1107 96-97 -0.19326588022250846 1107 98-99 -0.3953732547048254 1107 100-101 -1.2570726634561602 1108 1 -0.12874979417389198 1108 2 -0.07603071198349909 1108 3 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-0.5837211150126116 2316 100-101 0.49335457311068254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 49.0 6 74.0 7 187.0 8 520.0 9 1035.0 10 1722.0 11 2735.0 12 4238.0 13 5955.0 14 7536.0 15 10507.0 16 13958.0 17 20692.0 18 20638.0 19 13927.0 20 12467.0 21 13289.0 22 13845.0 23 15150.0 24 17159.0 25 19826.0 26 23415.0 27 28077.0 28 33483.0 29 40968.0 30 49773.0 31 61565.0 32 77626.0 33 101727.0 34 141982.0 35 214321.0 36 344647.0 37 515856.0 38 304356.0 39 12273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13859395214816 19.06088105941319 14.851003637847848 22.9495213505908 2 15.689285690982189 20.354511888993184 40.00134228313039 23.954860136894236 3 19.482400710664347 24.367146128957987 29.811782076605216 26.338671083772446 4 13.236449698405758 16.783293049303737 36.21615046248179 33.764106789808714 5 14.051140987267885 37.19340715269123 33.52328814624175 15.232163713799137 6 32.28046446724876 35.5425387234701 17.11066099054902 15.06633581873212 7 28.992336868322955 32.80297613998522 20.078924383784845 18.12576260790698 8 26.480356525313688 35.06984999053877 19.28012610004297 19.16966738410457 9 26.6612851055976 17.164212494605234 20.370964178751098 35.80353822104607 10-11 25.560170171672418 26.465092715410808 26.42899555275499 21.545741560161783 12-13 26.287240200634045 23.81345433767183 27.412078124606754 22.487227337087376 14-15 22.993486594502638 25.077333581906945 26.079745522761964 25.84943430082845 16-17 22.40225881317694 28.468531284380727 26.627023401675515 22.50218650076682 18-19 22.200692347935718 26.680167179538568 29.304634256891145 21.814506215634573 20-21 23.066296668807485 25.774376187897268 29.281789971840016 21.87753717145523 22-23 22.687730879689486 25.796887394563196 29.17459374962132 22.340787976126002 24-25 22.5654031408049 26.089994087891178 29.25969538074843 22.084907390555493 26-27 22.379505297059467 26.151300611037833 29.289872432452587 22.179321659450114 28-29 21.99989606619884 26.48375405161222 29.3707600660236 22.14558981616534 30-31 22.209892067386342 26.1007187739735 29.251215744981117 22.438173413659037 32-33 22.07604689976729 26.24488662129934 29.114181274489106 22.564885204444266 34-35 22.73516723495398 26.272959700932493 28.834704137116113 22.157168926997414 36-37 22.675107597863704 25.954628013486097 28.935830088614395 22.434434300035804 38-39 22.36027016311458 26.284520628758333 29.026009155077144 22.32920005304994 40-41 22.574732306100355 26.18381356455592 28.94746103013064 22.293993099213086 42-43 22.400189801701035 26.309862463683874 28.544949955649233 22.74499777896585 44-45 22.50445231928431 26.496368464202703 28.475780361881153 22.523398854631836 46-47 22.78366288235863 26.71620486300867 27.888808228452106 22.61132402618059 48-49 22.910335187654145 26.72349646089695 27.713881351899378 22.652286999549528 50-51 22.58667630507039 27.052757443371465 27.666995947722377 22.693570303835763 52-53 23.03131807542699 26.971431740708184 27.37994256146771 22.617307622397114 54-55 22.5004870437053 26.766057761053098 27.674825386675415 23.058629808566188 56-57 22.734925819031762 27.131649808503226 27.432731441940998 22.700692930524013 58-59 22.882674575688984 26.571071090082736 27.638551403557056 22.907702930671224 60-61 22.693731752879458 26.672606108287688 28.03025712826211 22.603405010570746 62-63 22.58125406797589 26.793375837976818 27.84167560174583 22.78369449230146 64-65 22.376387416838885 26.672497382935905 27.755584390645872 23.19553080957934 66-67 22.36336654613858 26.90691652241515 27.538220826470315 23.191496104975958 68-69 22.404453978622673 26.688798285712288 27.790896983100655 23.11585075256438 70-71 22.401250562489793 26.810518017267476 27.588204574536334 23.2000268457064 72-73 22.53784259679586 26.605403248884464 27.600580351025684 23.256173803294 74-75 22.38012432897495 26.98268734404115 27.331284797991053 23.30590352899285 76-77 22.39669980545927 26.80974196528124 27.444331960196127 23.349226269063358 78-79 22.42000488519785 26.802954985325762 27.514408536790015 23.262631592686375 80-81 22.30652425997114 27.047919641758543 27.453308923597774 23.192247174672538 82-83 22.44089992626764 26.83473325385277 27.346921464319042 23.377445355560546 84-85 22.298725110668446 26.719613832600515 27.36605365963242 23.615607397098618 86-87 22.384788533546274 26.661613782848704 27.41928974556187 23.534307938043153 88-89 22.17703098484501 26.69523690962137 27.269761812720606 23.85797029281301 90-91 22.17518203079769 26.700057896020247 27.40267313928884 23.72208693389323 92-93 22.211336886033635 26.51214253555419 27.44081137286668 23.835709205545495 94-95 22.002803881623624 26.39976193106161 27.506703262541848 24.09073092477292 96-97 22.09167327710037 26.283534939909526 27.47003399518456 24.154757787805544 98-99 21.787321148221487 26.383082746453024 27.169087943609156 24.660508161716336 100-101 22.211173738175475 25.26829988998382 27.965362497602648 24.555163874238062 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 450.0 1 326.0 2 225.0 3 282.5 4 289.0 5 300.5 6 516.5 7 1087.0 8 1787.0 9 2187.5 10 2207.0 11 2149.5 12 2232.0 13 2468.5 14 2789.0 15 2960.5 16 3115.0 17 3328.0 18 3535.0 19 3808.0 20 4192.0 21 4779.0 22 5545.0 23 6660.5 24 7987.0 25 9666.5 26 11926.5 27 14580.0 28 17145.5 29 20040.0 30 23106.5 31 26730.0 32 31680.0 33 34421.5 34 36271.5 35 39344.5 36 43141.5 37 47368.5 38 50165.5 39 52057.0 40 54608.5 41 59769.5 42 65023.5 43 69497.5 44 73730.0 45 77212.5 46 77932.5 47 79364.5 48 89687.0 49 93251.5 50 87573.5 51 84104.5 52 80411.0 53 79607.0 54 78417.0 55 71728.5 56 64185.0 57 57938.0 58 51767.0 59 45389.5 60 38282.0 61 31332.0 62 25946.0 63 20581.5 64 15708.0 65 12179.5 66 9103.5 67 7291.5 68 5481.0 69 3701.5 70 2777.5 71 2095.5 72 1707.0 73 1490.5 74 1245.5 75 1082.5 76 749.0 77 399.5 78 239.0 79 147.5 80 84.5 81 53.5 82 37.0 83 20.5 84 11.5 85 8.0 86 6.0 87 4.5 88 2.5 89 1.0 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4273866772806462 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001071962222187008 16-17 0.0021672279709432987 18-19 2.3303526569282783E-5 20-21 0.0 22-23 0.0 24-25 6.991057970784835E-5 26-27 0.0031692796134224588 28-29 6.991057970784835E-5 30-31 0.0 32-33 4.6607053138565565E-5 34-35 0.012257654975442745 36-37 0.026752448501536637 38-39 0.020949870385785222 40-41 0.00887864362289674 42-43 0.009368017680851678 44-45 0.004054813623055204 46-47 0.005383114637504323 48-49 0.0031925831399917414 50-51 6.758022705092007E-4 52-53 0.0 54-55 0.0 56-57 2.3303526569282783E-5 58-59 0.0014681221738648152 60-61 0.0025167808694825404 62-63 0.0013283010144491186 64-65 0.002982851400868196 66-67 0.0018642821255426226 68-69 0.004684008840425839 70-71 1.6312468598497947E-4 72-73 0.0 74-75 0.0 76-77 0.0973155269533249 78-79 0.015939612173389423 80-81 2.796423188313934E-4 82-83 0.0 84-85 0.0 86-87 0.10568149299169742 88-89 0.413684203657908 90-91 0.4198829417253372 92-93 0.0 94-95 4.6607053138565565E-5 96-97 0.0 98-99 6.991057970784835E-5 100-101 2.0973173912354506E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2145598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.27216897617043 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16268072722255 38.024814979719324 2 14.204459888315807 14.56586935217926 3 4.952001603772856 7.616995889967265 4 2.254865114966852 4.624473007722096 5 1.2363124721380652 3.1694210989404934 6 0.7279584021751415 2.2394403722368135 7 0.44745263251696504 1.6059306888269484 8 0.3279448668747488 1.345155570341587 9 0.23540644639526576 1.0862819187892083 >10 1.2622375238444314 11.886285547817165 >50 0.11185622701261524 3.955139821930604 >100 0.07081906615349984 6.750714596629701 >500 0.004913205227441811 1.6439504939875258 >1k 9.098528198966245E-4 0.7895534083637544 >5k 1.819705639793249E-4 0.6959732525482334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7908 0.36856857621977646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7011 0.3267620495544832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4305 0.20064336376152478 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2803 0.13063956994739928 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.6607053138565565E-5 8 0.0 0.0 0.0 0.0 4.6607053138565565E-5 9 0.0 0.0 0.0 0.0 4.6607053138565565E-5 10-11 0.0 0.0 0.0 0.0 4.6607053138565565E-5 12-13 0.0 0.0 0.0 0.0 4.6607053138565565E-5 14-15 0.0 0.0 0.0 0.0 4.6607053138565565E-5 16-17 0.0 0.0 0.0 6.991057970784835E-5 4.6607053138565565E-5 18-19 0.0 0.0 0.0 1.398211594156967E-4 4.6607053138565565E-5 20-21 0.0 0.0 0.0 1.8642821255426226E-4 4.6607053138565565E-5 22-23 0.0 0.0 0.0 2.796423188313934E-4 4.6607053138565565E-5 24-25 0.0 0.0 0.0 4.427670048163729E-4 4.6607053138565565E-5 26-27 0.0 0.0 0.0 8.389269564941802E-4 9.321410627713113E-5 28-29 0.0 0.0 0.0 0.002889637294591065 9.321410627713113E-5 30-31 0.0 0.0 0.0 0.009321410627713113 9.321410627713113E-5 32-33 0.0 0.0 0.0 0.020390585748122435 9.321410627713113E-5 34-35 0.0 0.0 0.0 0.035980645022972615 9.321410627713113E-5 36-37 0.0 0.0 0.0 0.06282630763078638 9.321410627713113E-5 38-39 0.0 0.0 0.0 0.10523872598688105 9.321410627713113E-5 40-41 0.0 0.0 0.0 0.15261479550223295 9.321410627713113E-5 42-43 0.0 0.0 0.0 0.2019250577228353 1.1651763284641391E-4 44-45 0.0 0.0 0.0 0.2628870832280791 1.398211594156967E-4 46-47 0.0 0.0 0.0 0.3403946125975136 1.398211594156967E-4 48-49 0.0 0.0 0.0 0.4247766823048866 1.8642821255426226E-4 50-51 0.0 0.0 0.0 0.5169421298864 1.8642821255426226E-4 52-53 0.0 0.0 0.0 0.611880697129658 2.3303526569282783E-4 54-55 0.0 0.0 0.0 0.716373710266322 2.796423188313934E-4 56-57 0.0 0.0 0.0 0.8427487348515426 2.796423188313934E-4 58-59 0.0 0.0 0.0 0.9867179219965716 2.796423188313934E-4 60-61 0.0 0.0 0.0 1.1494464480298734 2.796423188313934E-4 62-63 0.0 0.0 0.0 1.3128740798602534 3.26249371969959E-4 64-65 0.0 0.0 0.0 1.4817314333812766 3.26249371969959E-4 66-67 0.0 0.0 0.0 1.681955333664554 3.4955289853924175E-4 68-69 0.0 0.0 0.0 1.9209096951059799 3.728564251085245E-4 70-71 0.0 0.0 0.0 2.1789729483342173 3.728564251085245E-4 72-73 0.0 0.0 0.0 2.4412308363449258 3.728564251085245E-4 74-75 0.0 0.0 0.0 2.7153968264325377 3.728564251085245E-4 76-77 0.0 0.0 0.0 3.013145985408264 3.728564251085245E-4 78-79 0.0 0.0 0.0 3.3533541697932234 3.728564251085245E-4 80-81 0.0 0.0 0.0 3.734623167993259 3.728564251085245E-4 82-83 0.0 0.0 0.0 4.127474018898228 4.194634782470901E-4 84-85 0.0 0.0 0.0 4.525894412653256 4.194634782470901E-4 86-87 0.0 0.0 0.0 4.947198869499319 4.194634782470901E-4 88-89 0.0 0.0 0.0 5.4127567233004505 4.194634782470901E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13360 0.0 28.326012 1 GGTATCA 9625 0.0 27.532488 1 TATCAAC 15935 0.0 23.646313 2 TCAACGC 16180 0.0 23.288258 4 ATCAACG 16200 0.0 23.230211 3 CAACGCA 16245 0.0 23.165863 5 GTACATG 10260 0.0 22.762508 1 AACGCAG 16590 0.0 22.741322 6 GAGTACT 8050 0.0 21.87326 12-13 TACATGG 10420 0.0 21.769835 2 AGTACTT 8200 0.0 21.125866 12-13 ACATGGG 10410 0.0 20.742239 3 GTACTTT 8540 0.0 20.56266 14-15 ACGCAGA 18415 0.0 20.281404 7 CGCAGAG 18585 0.0 20.044815 8 GCAGAGT 19435 0.0 18.973244 9 AGAGTAC 15275 0.0 18.751307 10-11 TACTTTT 9400 0.0 18.454191 14-15 TAACGCA 130 0.007605356 18.25247 4 GTATAGA 630 0.0 18.156916 1 >>END_MODULE