FastQCFastQC Report
Fri 27 May 2016
SRR939209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576684
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112390.7128251444170169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81630.517732151781841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58920.3736956803011891No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56140.3560637388341608No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44750.28382351821925006No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39650.25147715077973776No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32580.20663620611359027No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23390.14834932047258678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21790.1382014404915633No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20490.12995628800698172No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20240.12837068175994684No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19770.12538974201552117No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18210.11549555903402331No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.11219749804019068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.10300098180738816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT77400.023.74412312-13
TGGACCG1601.9003019E-623.6919545
GTACATG98600.022.4853251
AGTACTT82200.021.63732312-13
TACATGG99350.021.6053432
GTACTTT85100.021.54039614-15
ACATGGG97650.021.1565633
TACGATG1605.085738E-520.730464
GTCTACG1404.870523E-420.3461571
CATGGGG32550.020.0890314
GTATTGA3800.019.9892081
TCGTATA1250.00611796618.9535622
TACTTTT94050.018.65957314-15
ACTTTTT101550.018.28575516-17
CATGGGA63650.018.2388974
GTATCAA210950.017.5764311
ATGGGAT24050.016.9439335
GTACACG1700.001794423916.755661
TAGGACC4255.820766E-1116.7237324
GTAGGAC3001.5047353E-615.7946373
Produced by FastQC (version 0.11.5)