FastQCFastQC Report
Fri 27 May 2016
SRR939209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576684
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.7077512044265053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.547478124976216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.3950696525112198No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54990.3487699500978002No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA45310.2873752762126083No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44200.2803351844757732No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31130.19743968988078778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22540.14295825923266806No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21380.13560104624642605No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20440.12963916675757475No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.12887807575899798No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20200.12811698476042124No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT19310.12247222652097695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.11232434653995348No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17100.10845546729718827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96950.024.2082711
TACATGG98500.023.4612062
GAGTACT82000.022.94566712-13
ACATGGG97250.022.7380873
CATGGGG34350.021.5504064
GTACTTT88550.021.16800514-15
AGTACTT86700.021.12708512-13
ACTTTTT102250.018.35471316-17
TAAGGTG6800.018.1435415
CATGGGA60600.018.0882664
ATGGGGA20500.018.0550355
ATGGGAT23550.017.9331535
TACTTTT100100.017.9196314-15
GTATCAA215550.017.1387211
GTATAGA3902.910383E-1017.089451
GTAAGGT6200.016.8379254
ACCGTCC1700.001800007416.7478838
GTGTAGC8350.016.5339341
TAGTACT3251.9352956E-716.0607914
CATGGGT12200.015.9470974
Produced by FastQC (version 0.11.5)