FastQCFastQC Report
Fri 27 May 2016
SRR939208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558712
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108800.6980122049487013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80150.5142066013477794No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56100.3599125431766741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.3551650336944862No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44970.28850743434322695No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39110.2509122916869826No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33520.2150493484364013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22670.14544059454216046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21250.1363305087790432No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20730.13299442103480308No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20550.13183962143102768No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18900.12125395839641961No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18470.11849527045406721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.11240049476747468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.10162236513223738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99150.024.7147751
TACATGG100300.023.947442
GAGTACT76400.023.1807812-13
ACATGGG98950.022.3597563
CATGGGG35500.021.7533044
AGTACTT80100.021.10500712-13
GTACTTT84450.020.83161514-15
GTACGTG1604.9990544E-520.7767561
ATGGGGA19200.019.7397125
ACCGCGC1200.004852532419.7289378
CATGGGA60800.018.5455364
TGGACCG1801.2587821E-418.423735
ACTTTTT97250.018.28483616-17
GTATCAA201550.017.860191
CCGTCTA1600.001216905417.7560449
TACTTTT96400.017.43873814-15
GTATTAC3401.7553248E-816.761081
CCATGCG1700.001826313816.711579
GGTATCA164700.016.5795961
GTAGGAC2901.0581261E-616.3368383
Produced by FastQC (version 0.11.5)