##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558712 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32443453312735 33.0 31.0 34.0 31.0 34.0 2 32.421381884530305 34.0 31.0 34.0 31.0 34.0 3 32.47967873475023 34.0 31.0 34.0 31.0 34.0 4 35.928092553338914 37.0 35.0 37.0 35.0 37.0 5 35.97236885325833 37.0 35.0 37.0 35.0 37.0 6 36.07376795713384 37.0 37.0 37.0 35.0 37.0 7 36.019272963831675 37.0 37.0 37.0 35.0 37.0 8 36.02111358608903 37.0 37.0 37.0 35.0 37.0 9 37.731539245222976 39.0 38.0 39.0 35.0 39.0 10-11 37.64567508301726 39.0 38.0 39.0 35.0 39.0 12-13 37.561229078880515 39.0 38.0 39.0 35.0 39.0 14-15 38.8562826230888 40.0 38.0 41.0 35.5 41.0 16-17 38.7855517889129 40.0 38.0 41.0 35.0 41.0 18-19 38.73700337201484 40.0 38.0 41.0 35.0 41.0 20-21 38.744596820965 40.0 38.5 41.0 35.0 41.0 22-23 38.78361942424258 40.0 39.0 41.0 35.0 41.0 24-25 38.733943794620174 40.5 38.5 41.0 35.0 41.0 26-27 38.61732026185722 40.0 38.0 41.0 35.0 41.0 28-29 38.47668844533179 40.0 38.0 41.0 34.0 41.0 30-31 38.25558409763959 40.0 38.0 41.0 34.0 41.0 32-33 38.02875707635535 40.0 38.0 41.0 34.0 41.0 34-35 37.719158831137506 40.0 37.5 41.0 33.0 41.0 36-37 37.445826425920885 40.0 37.0 41.0 33.0 41.0 38-39 37.24830565235912 40.0 37.0 41.0 32.5 41.0 40-41 37.04579325751005 40.0 36.5 41.0 31.5 41.0 42-43 36.82954420059639 40.0 36.0 41.0 31.0 41.0 44-45 36.52255227392873 40.0 36.0 41.0 30.5 41.0 46-47 36.26259597667818 40.0 36.0 41.0 30.0 41.0 48-49 36.08296914375459 40.0 36.0 41.0 29.0 41.0 50-51 34.942604535026355 38.5 34.5 40.0 26.5 40.5 52-53 35.07852444839072 38.5 35.0 39.5 27.5 40.5 54-55 35.599954321260114 39.0 35.0 41.0 28.0 41.0 56-57 35.526522218344375 39.0 35.0 41.0 27.5 41.0 58-59 35.33905140911214 39.0 35.0 41.0 27.0 41.0 60-61 35.045987327999015 39.0 35.0 41.0 26.0 41.0 62-63 34.6791697247471 38.0 35.0 40.0 26.0 41.0 64-65 34.30739193641929 37.5 34.0 40.0 25.0 41.0 66-67 33.921720304969746 37.0 34.0 40.0 23.5 41.0 68-69 33.52222026904265 36.5 34.0 39.0 22.5 41.0 70-71 33.086333459933584 36.0 33.5 39.0 21.5 41.0 72-73 32.638318367985875 35.0 33.0 38.5 20.0 40.0 74-75 32.19584054013826 35.0 33.0 37.5 18.5 39.5 76-77 31.751973103434118 35.0 32.0 37.0 17.0 39.0 78-79 31.328497182288967 35.0 32.0 36.5 12.0 39.0 80-81 30.888234324236933 35.0 31.5 36.0 7.0 38.5 82-83 30.491403479282894 35.0 31.0 35.5 4.0 37.0 84-85 30.107222180877546 35.0 31.0 35.0 2.0 37.0 86-87 29.700242572072327 34.0 30.5 35.0 2.0 36.0 88-89 29.265651704740836 34.0 30.0 35.0 2.0 36.0 90-91 28.85327148312196 34.0 29.5 35.0 2.0 36.0 92-93 28.45060954172419 34.0 29.0 35.0 2.0 35.0 94-95 27.98499209603827 34.0 29.0 35.0 2.0 35.0 96-97 27.390130761808464 34.0 27.0 35.0 2.0 35.0 98-99 26.69640222183444 34.0 25.5 35.0 2.0 35.0 100-101 23.7486536961286 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19473952433111918 1101 2 0.3434252039464809 1101 3 0.30476031858032826 1101 4 0.25712427272725336 1101 5 0.27745710695973 1101 6 0.2511500094824015 1101 7 0.29176259850924424 1101 8 0.2577486486534397 1101 9 0.30388846171100425 1101 10-11 0.3030478352237367 1101 12-13 0.3628784812418715 1101 14-15 0.4512889256712853 1101 16-17 0.43325419212510496 1101 18-19 0.4734119316798413 1101 20-21 0.5093718672078538 1101 22-23 0.5276342331648962 1101 24-25 0.5366695587733332 1101 26-27 0.5203925819457496 1101 28-29 0.6103104709326246 1101 30-31 0.558635287737701 1101 32-33 0.48308124207398606 1101 34-35 0.4759234082318855 1101 36-37 0.434831476995889 1101 38-39 0.20142529903817064 1101 40-41 0.22409946841445816 1101 42-43 0.21279815742451547 1101 44-45 0.3319038519045776 1101 46-47 0.28703629888289584 1101 48-49 0.21694049878822597 1101 50-51 0.1974167139732259 1101 52-53 0.06323119082740902 1101 54-55 0.028353147380336452 1101 56-57 0.048014684119301876 1101 58-59 0.09949931105688847 1101 60-61 0.07751787748652106 1101 62-63 0.1832845421893552 1101 64-65 0.05082257776862775 1101 66-67 0.100258873970553 1101 68-69 0.021122070728104347 1101 70-71 0.051183118378965275 1101 72-73 -0.16550265331120784 1101 74-75 -0.2059163051596613 1101 76-77 -0.2626985602289409 1101 78-79 -0.3085401675746198 1101 80-81 -0.35797614359143637 1101 82-83 -0.4187356550091508 1101 84-85 -0.4729488287239647 1101 86-87 -0.6309920033941303 1101 88-89 -0.597278592835444 1101 90-91 -0.6813368238864435 1101 92-93 -0.6601207628032668 1101 94-95 -0.6798658301413809 1101 96-97 -0.6156179889449795 1101 98-99 -0.7665621373583882 1101 100-101 -0.9614622365629621 1102 1 0.13117640229125271 1102 2 0.2416300811274752 1102 3 0.23045472041153658 1102 4 0.13658583158540694 1102 5 0.18115088164921644 1102 6 0.21672026652606746 1102 7 0.2865050791418824 1102 8 0.32726398744789975 1102 9 0.2728246795960274 1102 10-11 0.3976266059696627 1102 12-13 0.3720716808637903 1102 14-15 0.439223484501845 1102 16-17 0.31334936679308356 1102 18-19 0.38642163210989366 1102 20-21 0.383096261500981 1102 22-23 0.4826849477630546 1102 24-25 0.47890411991205184 1102 26-27 0.4248984319845661 1102 28-29 0.4533094243828302 1102 30-31 0.6052568572471273 1102 32-33 0.6666060487944279 1102 34-35 0.6058945901125625 1102 36-37 0.6760451594944072 1102 38-39 0.5960286150078176 1102 40-41 0.610081274548854 1102 42-43 0.5571310537102434 1102 44-45 0.578573217044621 1102 46-47 0.634862104357488 1102 48-49 0.6033901278794929 1102 50-51 0.5601858318032455 1102 52-53 0.5142108323658121 1102 54-55 0.459462979938003 1102 56-57 0.5749328004647865 1102 58-59 0.47687959257259394 1102 60-61 0.41855405748408003 1102 62-63 0.3708517108133549 1102 64-65 0.3552050261321682 1102 66-67 0.15025259692365722 1102 68-69 0.33228992233983945 1102 70-71 0.28147502076328834 1102 72-73 0.28704709439654863 1102 74-75 0.40375327446960085 1102 76-77 0.1136683863621144 1102 78-79 0.09128041584733282 1102 80-81 0.2643941819086315 1102 82-83 0.07771345052573864 1102 84-85 0.027795127999880265 1102 86-87 0.009457343089287917 1102 88-89 0.22305628057932125 1102 90-91 0.24858656236361298 1102 92-93 0.043595023772635244 1102 94-95 0.04554855347231879 1102 96-97 -0.03408956461886348 1102 98-99 -0.15748310680465138 1102 100-101 -0.3127181807574786 1103 1 0.01526612026650298 1103 2 0.07022522490662197 1103 3 0.02715374592172992 1103 4 -0.0021923715543508138 1103 5 0.025407563808045097 1103 6 -0.022839284298775908 1103 7 0.04443680898766189 1103 8 0.09802024793969366 1103 9 0.13855046566585116 1103 10-11 0.02025748902290303 1103 12-13 0.17437236005365975 1103 14-15 0.20982783844676334 1103 16-17 0.24673156206881686 1103 18-19 0.21668083096913193 1103 20-21 0.13880845313201462 1103 22-23 0.07029804631076075 1103 24-25 0.21712284937739668 1103 26-27 0.2678278606190787 1103 28-29 0.26584849841798786 1103 30-31 0.24434791870410066 1103 32-33 0.34128341365479287 1103 34-35 0.4310706394249948 1103 36-37 0.4058430519573406 1103 38-39 0.2705623204368166 1103 40-41 0.30546342193810716 1103 42-43 0.24967752354575623 1103 44-45 0.21890001564739947 1103 46-47 0.2666688345358139 1103 48-49 0.20209878921302504 1103 50-51 0.16124199465983935 1103 52-53 0.18107764677112215 1103 54-55 0.14046535031861396 1103 56-57 0.21859409409135822 1103 58-59 0.13701410036611605 1103 60-61 0.2208353160471077 1103 62-63 0.08376855594583077 1103 64-65 -0.031343928479429906 1103 66-67 0.007327956936855173 1103 68-69 -0.09197415561934719 1103 70-71 -0.014634202970320587 1103 72-73 0.024153793448654426 1103 74-75 -0.011745981959826679 1103 76-77 -0.12711679224113936 1103 78-79 -0.24650201460420718 1103 80-81 -0.18799575235387422 1103 82-83 -0.35177131758399227 1103 84-85 -0.1392134835849248 1103 86-87 -0.09987253887054592 1103 88-89 -0.16886739901757863 1103 90-91 -0.2610471326899777 1103 92-93 -0.26918188643223573 1103 94-95 -0.5691342385221283 1103 96-97 -0.5994899886802187 1103 98-99 -0.7527904980823941 1103 100-101 -0.8758384103997443 1104 1 0.14238844789481675 1104 2 0.14878242199475977 1104 3 0.212997056367783 1104 4 0.2152157412402147 1104 5 0.1999621146893844 1104 6 0.22419549109666548 1104 7 0.24481926856452674 1104 8 0.09533579517990631 1104 9 0.18092388575893636 1104 10-11 0.2429520717930984 1104 12-13 0.3299778478317492 1104 14-15 0.3707471885755851 1104 16-17 0.36747999411375787 1104 18-19 0.30298671076828754 1104 20-21 0.3334095710510212 1104 22-23 0.39974614163622135 1104 24-25 0.3277976062700745 1104 26-27 0.28071933254577175 1104 28-29 0.30602096701365866 1104 30-31 0.3706634633601382 1104 32-33 0.3928849631329214 1104 34-35 0.29560604148561964 1104 36-37 0.2572600905314957 1104 38-39 0.264685105466107 1104 40-41 0.3844490771197897 1104 42-43 0.5328717845847564 1104 44-45 0.39824047961221964 1104 46-47 0.6838981216363322 1104 48-49 0.5738998895595699 1104 50-51 0.48488445697594074 1104 52-53 0.41502946203281255 1104 54-55 0.49544649867933543 1104 56-57 0.4092419237624725 1104 58-59 0.4968573953070532 1104 60-61 0.5531379035928836 1104 62-63 0.5421267190028161 1104 64-65 0.5467682717226552 1104 66-67 0.438573870692224 1104 68-69 0.41163776161433674 1104 70-71 0.22179481330747564 1104 72-73 0.2584069248364429 1104 74-75 0.2556129020851543 1104 76-77 0.2756609496451574 1104 78-79 0.3041563068634012 1104 80-81 0.30232233514150053 1104 82-83 0.22687348342073577 1104 84-85 0.30020853730047037 1104 86-87 0.26360205470154696 1104 88-89 0.32095555837693723 1104 90-91 0.19720843816970302 1104 92-93 0.08133301269404569 1104 94-95 0.1814155471196166 1104 96-97 0.17249639782619397 1104 98-99 0.04586003981599163 1104 100-101 -0.15612907967815914 1105 1 0.16958661682527065 1105 2 0.2370752740671378 1105 3 0.2587395666772281 1105 4 0.2261345946787543 1105 5 0.2355864180302234 1105 6 0.24444182630363542 1105 7 0.26886445796404956 1105 8 0.1933361136526699 1105 9 0.3047175213635924 1105 10-11 0.21528239451711784 1105 12-13 0.26544941322457305 1105 14-15 0.3780336171218508 1105 16-17 0.33651918362538424 1105 18-19 0.4629282447255676 1105 20-21 0.42407931132706267 1105 22-23 0.37209184232838055 1105 24-25 0.39352229797901117 1105 26-27 0.5002455323869341 1105 28-29 0.41114847765297924 1105 30-31 0.46500184116307963 1105 32-33 0.44714314731236016 1105 34-35 0.4582042434538991 1105 36-37 0.49656359755153545 1105 38-39 0.5374133703203583 1105 40-41 0.5189013808634328 1105 42-43 0.49211793674429316 1105 44-45 0.4942084259738664 1105 46-47 0.4444477798237898 1105 48-49 0.6038402974527344 1105 50-51 0.5073643180384764 1105 52-53 0.6233957215801738 1105 54-55 0.6298221606140544 1105 56-57 0.5271775456351051 1105 58-59 0.50988675085609 1105 60-61 0.598356074795305 1105 62-63 0.3725724667713948 1105 64-65 0.4757662085946066 1105 66-67 0.44819948661704956 1105 68-69 0.49509325058163967 1105 70-71 0.369744260469524 1105 72-73 0.5079603436811979 1105 74-75 0.528045017036952 1105 76-77 0.5157839535476896 1105 78-79 0.5972633558759632 1105 80-81 0.8059701380019071 1105 82-83 0.7560638433197582 1105 84-85 0.6745777305673002 1105 86-87 0.7560348542209603 1105 88-89 0.9213032492758124 1105 90-91 0.9888729345586071 1105 92-93 0.6401978904565624 1105 94-95 0.6723214632223851 1105 96-97 0.8706514385009179 1105 98-99 0.8396938218387469 1105 100-101 0.6972251280067567 1106 1 0.06105387361123604 1106 2 0.09519015068784142 1106 3 0.177034357487436 1106 4 0.15780396637553906 1106 5 0.05974046082717166 1106 6 0.15502003777286433 1106 7 0.028966047400793116 1106 8 0.012487156929829268 1106 9 3.553218677438963E-4 1106 10-11 0.11808251630284872 1106 12-13 0.013485000876336528 1106 14-15 0.10436294112081868 1106 16-17 0.08941670074497665 1106 18-19 0.08880381713986907 1106 20-21 0.22399661672757532 1106 22-23 0.16395153034982712 1106 24-25 0.2128011117746169 1106 26-27 0.23165551568177278 1106 28-29 0.18439713095646226 1106 30-31 0.18039134887609265 1106 32-33 0.261917694095132 1106 34-35 0.2731965700980439 1106 36-37 0.284058188678749 1106 38-39 0.30251487188518666 1106 40-41 0.4307420980502812 1106 42-43 0.4950542837519194 1106 44-45 0.3924126089621396 1106 46-47 0.4918418549765988 1106 48-49 0.5062485579829925 1106 50-51 0.6557308265831878 1106 52-53 0.5956578819848986 1106 54-55 0.514593814748288 1106 56-57 0.6136587123081796 1106 58-59 0.5565868753492325 1106 60-61 0.6867238752947031 1106 62-63 0.6384949770393504 1106 64-65 0.6890743393119294 1106 66-67 0.49483881324267287 1106 68-69 0.5292500150331207 1106 70-71 0.4024642003176311 1106 72-73 0.4570770409130489 1106 74-75 0.5400604330962366 1106 76-77 0.5872627714281506 1106 78-79 0.5587844805225153 1106 80-81 0.4373814037517576 1106 82-83 0.5700439804664477 1106 84-85 0.44940712842617003 1106 86-87 0.36375988826539185 1106 88-89 0.37293735820944107 1106 90-91 0.4767038613888488 1106 92-93 0.39163198390573584 1106 94-95 0.2583352462889401 1106 96-97 0.29064073237643484 1106 98-99 0.3036919846031907 1106 100-101 0.2470831792356094 1107 1 0.14065770351501783 1107 2 0.18651855413922647 1107 3 0.17052606061382392 1107 4 0.07531487185091379 1107 5 0.18622312120242412 1107 6 0.09819478350486577 1107 7 0.16049629888594552 1107 8 0.18678985761351186 1107 9 0.14353090175912797 1107 10-11 0.09039537612097348 1107 12-13 0.12438371029274009 1107 14-15 0.08584807848601628 1107 16-17 0.0030670702544171036 1107 18-19 0.09669635516710429 1107 20-21 0.035846406866980374 1107 22-23 0.22966614310755062 1107 24-25 0.16227654137828296 1107 26-27 0.23531052404421615 1107 28-29 0.32198824549072214 1107 30-31 0.12013700441183062 1107 32-33 0.07118043159305643 1107 34-35 0.10985472370479243 1107 36-37 0.2714269302242016 1107 38-39 0.28708338672465317 1107 40-41 0.2760650822461983 1107 42-43 0.35969014305889146 1107 44-45 0.39461733855316083 1107 46-47 0.27014615724630175 1107 48-49 0.27980765191907153 1107 50-51 0.17567071149699842 1107 52-53 0.20221154786050732 1107 54-55 0.17790136792246614 1107 56-57 0.12514647727542894 1107 58-59 0.17599887640026424 1107 60-61 0.06325762793215262 1107 62-63 0.21813170989656072 1107 64-65 0.22939181110459117 1107 66-67 0.2764484720668463 1107 68-69 0.24188372962363047 1107 70-71 0.3680785285806607 1107 72-73 0.40305015966351476 1107 74-75 0.38238484602181444 1107 76-77 0.2320767793739833 1107 78-79 0.27740769119271036 1107 80-81 0.17658564350584882 1107 82-83 0.16290515178378584 1107 84-85 0.2356468352469534 1107 86-87 0.14650771122525796 1107 88-89 0.2499725333203493 1107 90-91 0.3323833977445254 1107 92-93 0.3506578433100245 1107 94-95 0.5211123667800202 1107 96-97 0.5396641866211702 1107 98-99 0.6117120563316298 1107 100-101 0.4924729425243477 1108 1 0.10009108579127712 1108 2 0.019011056002923965 1108 3 0.1236222007906278 1108 4 0.2017196471868843 1108 5 0.21037185435411487 1108 6 0.14934080812569306 1108 7 0.09106535708802 1108 8 0.08563669826094866 1108 9 0.2822394219624158 1108 10-11 0.16447122832133942 1108 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-1.4339750756019036 2316 98-99 -1.7141713420014746 2316 100-101 -1.4810321048639423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4000.0 3 792.0 4 816.0 5 919.0 6 1000.0 7 1196.0 8 1540.0 9 1970.0 10 2543.0 11 3502.0 12 5372.0 13 9395.0 14 8089.0 15 8511.0 16 11284.0 17 18007.0 18 14253.0 19 8337.0 20 8022.0 21 8076.0 22 8388.0 23 9290.0 24 10289.0 25 11729.0 26 13729.0 27 15783.0 28 18812.0 29 22672.0 30 27601.0 31 34500.0 32 44052.0 33 59095.0 34 82523.0 35 128731.0 36 204959.0 37 336643.0 38 348849.0 39 63436.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13484136026417 19.30243986322906 14.780935279831054 21.781783496675725 2 14.854166091312134 21.06407726085429 40.969074720730944 23.112681927102635 3 19.39286505650343 26.16987318439163 30.286611624389444 24.150650134715494 4 12.393331213607356 17.03469606020022 38.05126525158899 32.520707474603434 5 13.095927861076817 38.30247678853835 33.93613973511891 14.665455615265929 6 30.025602604943074 36.72221173636764 18.12161108949741 15.130574569191873 7 27.26241939822909 34.4118117268474 20.95450217431363 17.371266700609887 8 25.48712690627787 34.47996245387792 20.373586482882423 19.659324156961794 9 26.799241633864273 17.646008915232102 21.16555456326213 34.389194887641494 10-11 24.54595736170707 26.99422601768565 27.169304127111715 21.290512493495562 12-13 26.401069383098342 24.73702812812694 27.671732261296466 21.19017022747825 14-15 23.235026458593143 25.699872722691154 27.25089037940836 23.81421043930734 16-17 21.093925049740207 29.170649842417113 27.950979492075273 21.784445615767407 18-19 20.973583776578668 27.745766803728138 30.714943381882716 20.565706037810475 20-21 22.204786644061766 26.568311171992953 30.333882404230682 20.893019779714596 22-23 21.643651512977065 26.517881250989266 30.17773535510952 21.66073188092415 24-25 21.636578713642876 26.72864714348007 30.466709522546537 21.16806462033051 26-27 21.64803195622716 26.58342591819565 30.722996795386383 21.045545330190798 28-29 21.0101134642195 26.99098592309041 31.007313622705944 20.991586989984146 30-31 21.219963707953042 27.073147866333287 30.55912996687998 21.1477584588337 32-33 20.94090716459695 27.223606313166986 30.349593548138543 21.485892974097524 34-35 21.662973494448977 26.814425739881592 30.184465503840418 21.338135261829013 36-37 21.902158823664088 26.50554466869198 30.43190984322594 21.16038666441799 38-39 21.665136850073782 26.735971065993613 30.4860045156188 21.112887568313806 40-41 21.41157875160712 26.902720527351764 30.683456472110297 21.002244248930815 42-43 21.18988304091682 27.32867930088278 29.829482896865994 21.65195476133441 44-45 21.325375598321394 27.386409620218068 29.541188855946615 21.747025925513924 46-47 21.58562823898724 27.4462343836199 29.02077756006746 21.947359817325392 48-49 21.76147358163672 27.320617315757957 28.993855024759508 21.92405407784581 50-51 21.816094696688776 27.854588604023512 28.870301521634683 21.459015177653026 52-53 21.71252333676197 28.030683609935092 28.733124175756853 21.523668877546083 54-55 21.15147198557081 27.700289957314077 28.956621186390674 22.191616870724435 56-57 21.612651289203683 28.253791638073388 28.274743031163744 21.85881404155918 58-59 21.73911221795321 27.5736039431196 28.630128200376383 22.057155638550807 60-61 21.62749225112601 27.45887829935168 29.141983338068666 21.771646111453645 62-63 21.904108555670824 27.40790840389611 28.952670661122653 21.73531237931041 64-65 21.60185635738233 27.5354733940274 29.07355938663589 21.78911086195438 66-67 21.39206958670959 27.64279923194559 28.785044739749654 22.180086441595165 68-69 21.369020509955657 27.815651662329522 28.868243177862613 21.94708464985221 70-71 21.469117884212224 27.709046927914855 28.676890824060635 22.14494436381229 72-73 21.557575034425376 27.473580336094887 28.498086045709165 22.47075858377057 74-75 21.412886541251762 28.091999454334545 28.337806908602005 22.157307095811696 76-77 21.61544942217172 27.503613542619494 28.543133753148048 22.33780328206074 78-79 21.51631034951808 27.6054301431221 28.641890467530473 22.23636903982934 80-81 21.40028689779669 28.066373321728477 28.40978031365343 22.123559466821398 82-83 21.473489004301815 27.813920888231763 28.344950032449812 22.367640075016613 84-85 21.507409772730863 27.518079948625317 28.276546406625325 22.697963872018498 86-87 21.65136833852161 27.48927741512845 28.583071340329557 22.27628290602038 88-89 21.87390780064097 27.097087253428942 28.39324980493561 22.635755140994473 90-91 21.531444404206283 27.2084543568632 28.68511609713184 22.574985141798678 92-93 21.672027714477927 27.444963528319814 28.585167129886134 22.297841627316124 94-95 21.595474101303843 27.256298072597424 28.43575867592374 22.712469150174996 96-97 21.490839948710935 26.926629292040648 28.420152370829214 23.162378388419196 98-99 21.658995686363543 27.001506676848773 28.122516821118243 23.216980815669437 100-101 22.18020446872726 26.305270281554773 28.746914091731394 22.767611157986575 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2773.0 1 1663.5 2 724.0 3 927.0 4 810.0 5 769.5 6 1362.0 7 2878.5 8 4589.0 9 4995.0 10 4015.0 11 3226.5 12 3260.0 13 3368.0 14 3447.0 15 3566.0 16 3671.5 17 3685.5 18 3660.0 19 3810.0 20 4046.5 21 4581.0 22 5262.0 23 6092.0 24 7177.0 25 8522.5 26 10569.0 27 13240.0 28 15354.0 29 17218.5 30 19576.5 31 22334.0 32 25852.0 33 29376.5 34 32815.5 35 35715.0 36 38925.5 37 42389.0 38 43735.0 39 43934.0 40 44433.0 41 45775.0 42 47207.0 43 48997.0 44 51204.0 45 52809.5 46 53532.0 47 57583.0 48 76710.0 49 76735.0 50 58096.5 51 51442.5 52 46569.5 53 44938.0 54 43847.0 55 40661.5 56 36789.5 57 32960.5 58 29825.0 59 26621.0 60 22849.5 61 19182.5 62 15839.0 63 12263.5 64 9035.0 65 6826.0 66 5197.0 67 4255.5 68 3292.0 69 2369.0 70 1878.5 71 1420.0 72 1112.5 73 987.0 74 825.0 75 646.0 76 468.5 77 304.0 78 209.5 79 153.0 80 99.0 81 60.0 82 41.0 83 31.0 84 20.0 85 16.0 86 17.0 87 11.0 88 8.0 89 7.0 90 7.0 91 4.5 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38769188920082737 2 0.10912856255677764 3 0.11079660642889771 4 0.12234460246665195 5 0.1243334240064874 6 0.06787655448857774 7 0.07756404005358271 8 0.0744845744435149 9 0.07397133017517027 10-11 0.07195043086856327 12-13 0.0756072962805188 14-15 0.06989745379518475 16-17 0.07179004203470558 18-19 0.08083597226427974 20-21 0.07907169509184507 22-23 0.08779684765370382 24-25 0.09976185465948809 26-27 0.10149405406515123 28-29 0.09501434517730023 30-31 0.08635334814898454 32-33 0.08545517067938144 34-35 0.33165203065094767 36-37 0.5240544757466421 38-39 0.5050644378178907 40-41 0.47561704792161735 42-43 0.5049361267508045 44-45 0.5144953012487233 46-47 0.5117366133063709 48-49 0.5193711217979974 50-51 0.5181842444274504 52-53 0.516259578421158 54-55 0.51109505797094 56-57 0.48132689040695137 58-59 0.47163940484194644 60-61 0.4202829002407115 62-63 0.49585811875445884 64-65 0.5227713650757806 66-67 0.5325550839411001 68-69 0.5477599453908099 70-71 0.5428841248415358 72-73 0.5297322404652046 74-75 0.5332287170433024 76-77 0.5099402583671647 78-79 0.4823854567104122 80-81 0.4899558096684955 82-83 0.5039737937476583 84-85 0.49742992932626423 86-87 0.49913005096515584 88-89 0.5095232473991347 90-91 0.5274547190244253 92-93 0.5344797499473924 94-95 0.5297643182319761 96-97 0.5314644398708678 98-99 0.5312078177366955 100-101 0.5076306591596138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558712.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.617947296994274 #Duplication Level Percentage of deduplicated Percentage of total 1 70.68174457888688 33.65719588217046 2 14.930320132453806 14.219023943888756 3 5.83560216662582 8.336381892498416 4 2.925730887669668 5.572691968569701 5 1.5682815470666418 3.733917402753399 6 0.9574608930551158 2.7355393406658948 7 0.6393846562247886 2.131232940383315 8 0.43931115770439366 1.6735276443639486 9 0.3130595626383353 1.3416528379079093 >10 1.541272317263902 13.26059654053685 >50 0.11105082438865085 3.650449939019803 >100 0.050712133379741245 4.378664897493058 >500 0.0031020062394132887 1.0725757582083582 >1k 0.002427657056932139 2.307248469516405 >5k 4.046095094886898E-4 1.2305624874576582 >10k+ 1.3486983649622995E-4 0.6987380545660102 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10880 0.6980122049487013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8015 0.5142066013477794 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5610 0.3599125431766741 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5536 0.3551650336944862 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4497 0.28850743434322695 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3911 0.2509122916869826 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3352 0.2150493484364013 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2267 0.14544059454216046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2125 0.1363305087790432 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2073 0.13299442103480308 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2055 0.13183962143102768 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1890 0.12125395839641961 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1847 0.11849527045406721 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.11240049476747468 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.10162236513223738 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 6.415553354307915E-5 0.0 9 0.0 0.0 0.0 6.415553354307915E-5 0.0 10-11 0.0 0.0 0.0 9.623330031461873E-5 0.0 12-13 0.0 0.0 0.0 2.566221341723166E-4 0.0 14-15 0.0 0.0 0.0 2.886999009438562E-4 0.0 16-17 0.0 0.0 0.0 3.207776677153958E-4 0.0 18-19 0.0 0.0 0.0 3.8493320125847496E-4 0.0 20-21 0.0 0.0 0.0 6.09477568659252E-4 0.0 22-23 0.0 0.0 0.0 0.0010264885366892665 0.0 24-25 0.0 0.0 0.0 0.0015718105718054392 0.0 26-27 0.0 0.0 0.0 0.002630376875266245 0.0 28-29 0.0 0.0 0.0 0.0064155533543079155 0.0 30-31 0.0 0.0 0.0 0.017129527456002137 0.0 32-33 0.0 0.0 0.0 0.036279954218611264 0.0 34-35 0.0 0.0 0.0 0.061878012102299845 0.0 36-37 0.0 0.0 0.0 0.09989016572657425 0.0 38-39 0.0 0.0 0.0 0.15487145797299307 0.0 40-41 0.0 0.0 0.0 0.2190590692828438 0.0 42-43 0.0 0.0 0.0 0.2827655140911214 0.0 44-45 0.0 0.0 0.0 0.3598163098763595 0.0 46-47 0.0 0.0 0.0 0.4580063539640421 0.0 48-49 0.0 0.0 0.0 0.5685142604919959 0.0 50-51 0.0 0.0 0.0 0.6881643305498386 0.0 52-53 0.0 0.0 0.0 0.8073011563393366 0.0 54-55 0.0 0.0 0.0 0.9423806322142898 0.0 56-57 0.0 0.0 0.0 1.096610534851852 0.0 58-59 0.0 0.0 0.0 1.2713060526896567 0.0 60-61 0.0 0.0 0.0 1.4779189484651432 0.0 62-63 0.0 0.0 0.0 1.6861998881127493 0.0 64-65 0.0 0.0 0.0 1.9006397589804915 0.0 66-67 0.0 0.0 0.0 2.150653873197871 0.0 68-69 0.0 0.0 0.0 2.4291851220751495 0.0 70-71 0.0 0.0 0.0 2.7490325345541704 0.0 72-73 0.0 0.0 0.0 3.078246654930481 0.0 74-75 0.0 0.0 0.0 3.411278029552605 0.0 76-77 0.0 0.0 0.0 3.7691375956559003 0.0 78-79 0.0 0.0 0.0 4.178097044226259 0.0 80-81 0.0 0.0 0.0 4.6387337750655675 0.0 82-83 6.415553354307915E-5 0.0 0.0 5.107967347399648 0.0 84-85 6.415553354307915E-5 0.0 0.0 5.606038832061342 0.0 86-87 6.415553354307915E-5 0.0 0.0 6.134616272922772 0.0 88-89 6.415553354307915E-5 0.0 0.0 6.6885672273004895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9915 0.0 24.714775 1 TACATGG 10030 0.0 23.94744 2 GAGTACT 7640 0.0 23.18078 12-13 ACATGGG 9895 0.0 22.359756 3 CATGGGG 3550 0.0 21.753304 4 AGTACTT 8010 0.0 21.105007 12-13 GTACTTT 8445 0.0 20.831615 14-15 GTACGTG 160 4.9990544E-5 20.776756 1 ATGGGGA 1920 0.0 19.739712 5 ACCGCGC 120 0.0048525324 19.728937 8 CATGGGA 6080 0.0 18.545536 4 TGGACCG 180 1.2587821E-4 18.42373 5 ACTTTTT 9725 0.0 18.284836 16-17 GTATCAA 20155 0.0 17.86019 1 CCGTCTA 160 0.0012169054 17.756044 9 TACTTTT 9640 0.0 17.438738 14-15 GTATTAC 340 1.7553248E-8 16.76108 1 CCATGCG 170 0.0018263138 16.71157 9 GGTATCA 16470 0.0 16.579596 1 GTAGGAC 290 1.0581261E-6 16.336838 3 >>END_MODULE