FastQCFastQC Report
Fri 27 May 2016
SRR939208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558712
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108480.6959592278753227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86050.5520583661381961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59820.3837784016546995No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54490.3495835022762383No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44750.2870960126052792No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43020.2759971053023265No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29450.1889380462843681No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21240.13626635324550013No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20860.13382844297086313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20690.13273779890063078No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.12779782281781368No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19910.1277336672842706No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT18890.12118980286287652No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16610.10656234121505448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.10341872007144359No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC15740.1009808097968066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT81050.023.18794312-13
GTACATG90550.022.8362351
AGTACTT84850.022.23337212-13
TACATGG92050.021.96092
ACATGGG90550.021.2241783
GTACTTT89400.021.12835114-15
GACCGTC2451.7753882E-719.3685997
CTACCGT1507.7742507E-418.9812286
TACTTTT100500.018.84199514-15
ATGGGAT21250.018.534615
CATGGGA56000.018.4728014
ACTTTTT102700.018.39068216-17
GTAAGCG2600.018.25176694-95
TACCGTG1851.5333357E-417.9552177
CATGGGG33050.017.6602954
TATACAG10300.017.046255
GTATCAA209800.016.9871031
ATGGGGA18200.016.4260625
ACCGTCC1750.0021845816.2696238
AGTACAT72150.016.1794262
Produced by FastQC (version 0.11.5)