##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558712 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38469197645235 34.0 31.0 34.0 31.0 34.0 2 32.604737757841086 34.0 31.0 34.0 31.0 34.0 3 32.70357962214957 34.0 31.0 34.0 31.0 34.0 4 36.192824588506404 37.0 35.0 37.0 35.0 37.0 5 36.15490802662711 37.0 35.0 37.0 35.0 37.0 6 36.20719542802006 37.0 37.0 37.0 35.0 37.0 7 36.16070127130605 37.0 36.0 37.0 35.0 37.0 8 36.14091249698469 37.0 36.0 37.0 35.0 37.0 9 37.8529670651153 39.0 38.0 39.0 35.0 39.0 10-11 37.763071048404065 39.0 38.0 39.0 35.0 39.0 12-13 37.670790370511035 39.0 37.0 39.0 35.0 39.0 14-15 39.00003881409779 40.0 38.0 41.0 35.5 41.0 16-17 39.116312699202936 40.0 38.5 41.0 36.0 41.0 18-19 39.19199858601204 40.5 39.0 41.0 36.0 41.0 20-21 39.17091515302378 41.0 39.0 41.0 36.0 41.0 22-23 39.092298641442426 40.0 39.0 41.0 35.0 41.0 24-25 39.00333833318791 40.0 38.5 41.0 35.0 41.0 26-27 38.82301573350304 40.0 38.0 41.0 35.0 41.0 28-29 38.65139262416662 40.0 38.0 41.0 34.5 41.0 30-31 38.420772406961646 40.0 38.0 41.0 34.0 41.0 32-33 38.26298700465513 40.0 38.0 41.0 34.0 41.0 34-35 38.126198746144254 40.0 38.0 41.0 34.0 41.0 36-37 37.93712661479478 40.0 37.0 41.0 33.0 41.0 38-39 37.77462898854952 40.0 37.0 41.0 33.0 41.0 40-41 37.547245097234125 40.0 37.0 41.0 33.0 41.0 42-43 37.317164107288576 40.0 37.0 41.0 32.5 41.0 44-45 36.98485416164115 40.0 36.0 41.0 31.5 41.0 46-47 36.56513165998594 40.0 36.0 41.0 30.5 41.0 48-49 36.12673444484933 39.0 35.5 41.0 29.5 41.0 50-51 35.823265940083864 39.0 35.0 40.5 28.5 41.0 52-53 35.512258839349414 39.0 35.0 40.0 28.0 41.0 54-55 35.245011586489355 38.5 35.0 40.0 27.0 41.0 56-57 34.9928838682194 38.0 34.5 40.0 26.5 41.0 58-59 34.643996132704444 38.0 34.0 40.0 26.0 41.0 60-61 34.23072799850132 37.5 34.0 40.0 25.0 41.0 62-63 33.81053074589789 37.0 33.0 40.0 23.5 41.0 64-65 33.31618573540205 36.0 33.0 39.0 22.0 40.5 66-67 32.86254003305294 36.0 32.0 39.0 21.5 40.0 68-69 32.35797408373067 35.0 32.0 38.5 19.5 40.0 70-71 31.818994464660566 35.0 31.0 38.0 18.0 40.0 72-73 31.194616452558265 35.0 31.0 37.0 13.0 39.0 74-75 30.628141696477606 34.0 30.0 36.5 8.0 39.0 76-77 28.63090840386165 32.5 27.0 34.5 7.0 36.5 78-79 29.621510901308262 34.0 29.0 35.0 4.5 37.0 80-81 29.62984534667084 34.0 29.0 35.0 2.0 37.0 82-83 29.21350544552169 34.0 29.0 35.0 2.0 37.0 84-85 28.69834356827945 34.0 29.0 35.0 2.0 36.0 86-87 27.914997446609767 33.0 27.0 35.0 2.0 36.0 88-89 27.158791361072474 33.0 25.5 35.0 2.0 35.0 90-91 26.51660056508194 32.0 25.0 34.0 2.0 35.0 92-93 25.991579265444805 32.0 24.0 34.0 2.0 35.0 94-95 25.345404410821242 32.0 21.5 34.0 2.0 35.0 96-97 24.385914780921684 31.0 12.5 34.0 2.0 35.0 98-99 22.996409535565263 31.0 2.0 34.0 2.0 35.0 100-101 17.25883453774655 23.0 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2962320005791099 1101 2 0.23737092086130218 1101 3 0.18911539034832714 1101 4 0.13518267194583444 1101 5 0.19803107371910755 1101 6 0.20072700508956132 1101 7 0.13199518990785464 1101 8 0.2352536187003409 1101 9 0.26487062580852694 1101 10-11 0.2905517769933894 1101 12-13 0.28131426904965195 1101 14-15 0.2785117956329941 1101 16-17 0.26656597936828774 1101 18-19 0.28798510908040953 1101 20-21 0.3506984453802531 1101 22-23 0.3512016478533724 1101 24-25 0.24495520374613733 1101 26-27 0.2919210238873404 1101 28-29 0.3989707127799136 1101 30-31 0.4227046866189639 1101 32-33 0.4526351004943834 1101 34-35 0.6465206660584357 1101 36-37 0.6408239458608946 1101 38-39 0.6973827338273537 1101 40-41 0.6487551060800314 1101 42-43 0.7564105027998167 1101 44-45 0.704695933693614 1101 46-47 0.8408770741404794 1101 48-49 0.6705557423430193 1101 50-51 0.5960280291849998 1101 52-53 0.6632706376760567 1101 54-55 0.7020919628843316 1101 56-57 0.644115356311012 1101 58-59 0.4987979108087899 1101 60-61 0.5910008896769412 1101 62-63 0.5791258251757867 1101 64-65 0.5177359547156968 1101 66-67 0.7973399678009159 1101 68-69 0.7999477391721328 1101 70-71 0.8269990427653191 1101 72-73 0.6691126631168842 1101 74-75 0.423527368412163 1101 76-77 0.4712234143814804 1101 78-79 0.3618238722937086 1101 80-81 0.5759064586634146 1101 82-83 0.46209044935721266 1101 84-85 0.3183496946153994 1101 86-87 0.5196125055565339 1101 88-89 0.29752371875610706 1101 90-91 0.425521551396546 1101 92-93 0.16925753923029774 1101 94-95 0.08929385401422962 1101 96-97 0.22327811196645087 1101 98-99 0.03984998092034786 1101 100-101 -0.6678568360586219 1102 1 0.059111628212306755 1102 2 0.11401090491816745 1102 3 0.16408489178191132 1102 4 0.10741222833595288 1102 5 0.13096800544229126 1102 6 0.1435360708341662 1102 7 0.12817716909733434 1102 8 0.19238110693017774 1102 9 0.12951779103222094 1102 10-11 0.1932499501194087 1102 12-13 0.19795396036531798 1102 14-15 0.31439285938695605 1102 16-17 0.23311494906000263 1102 18-19 0.17106539954723843 1102 20-21 0.2691249839463694 1102 22-23 0.0938165473671404 1102 24-25 0.20307660464789734 1102 26-27 0.21792899658134246 1102 28-29 0.2955033058533161 1102 30-31 0.22051509503234712 1102 32-33 0.2654138731543796 1102 34-35 0.2750301123103043 1102 36-37 0.4315556468617885 1102 38-39 0.4109039432088011 1102 40-41 0.541482345029479 1102 42-43 0.6616251243220361 1102 44-45 0.4746990243651368 1102 46-47 0.26756961087245656 1102 48-49 0.33174129916773865 1102 50-51 0.3010662178375654 1102 52-53 0.22778376120778177 1102 54-55 0.10596701193255598 1102 56-57 0.2187919476549851 1102 58-59 -0.008208174267437585 1102 60-61 -0.09702256114507435 1102 62-63 -0.20485706522697456 1102 64-65 -0.012548685435000095 1102 66-67 0.2978553612028705 1102 68-69 0.261109144847822 1102 70-71 0.12231918060138369 1102 72-73 0.22466312072204886 1102 74-75 0.199006126941768 1102 76-77 0.07377769394820177 1102 78-79 0.2944644035514301 1102 80-81 0.3798520470816733 1102 82-83 0.12032494969583851 1102 84-85 0.2718007474422386 1102 86-87 0.4053359112851389 1102 88-89 0.2978859859916696 1102 90-91 0.49450826713864515 1102 92-93 0.3002358634332971 1102 94-95 0.3843257543606171 1102 96-97 0.10408825072014949 1102 98-99 0.3864020806910524 1102 100-101 -0.4257300262002275 1103 1 0.07639598609455334 1103 2 0.03192018132689611 1103 3 0.10058582689839568 1103 4 0.05329306017726765 1103 5 0.06842294559749718 1103 6 0.08186094865713756 1103 7 0.0561564395734635 1103 8 0.08841956509202475 1103 9 0.012475791931422009 1103 10-11 0.09661477324538481 1103 12-13 0.09603876731630123 1103 14-15 0.005775421792350244 1103 16-17 0.09941989546705798 1103 18-19 0.1756781974742836 1103 20-21 0.13642596907762083 1103 22-23 0.11614388336260362 1103 24-25 0.03657638782856765 1103 26-27 0.05421125011895356 1103 28-29 0.10497551706460939 1103 30-31 0.07173872754088251 1103 32-33 0.0559669353615746 1103 34-35 0.11898510966324238 1103 36-37 0.06503787166751351 1103 38-39 0.11998907206333342 1103 40-41 0.00881445637085676 1103 42-43 0.01655783051987214 1103 44-45 0.017420944932112548 1103 46-47 0.11048680185170667 1103 48-49 -0.006228420616558594 1103 50-51 -0.06856284818990588 1103 52-53 -0.0365583717091198 1103 54-55 -0.19684844688639913 1103 56-57 -0.15345464641932693 1103 58-59 -0.3040683883199904 1103 60-61 -0.10276015921258619 1103 62-63 -0.2003224818792262 1103 64-65 -0.16251849035080568 1103 66-67 -0.3661505022302194 1103 68-69 -0.31743345682448165 1103 70-71 -0.6333355472571043 1103 72-73 -0.3627009681446154 1103 74-75 -0.6623228824200389 1103 76-77 -0.5649584755060779 1103 78-79 -0.45479641546694793 1103 80-81 -0.34327951569350645 1103 82-83 -0.357298231615669 1103 84-85 -0.7017268098951668 1103 86-87 -0.5422041869885526 1103 88-89 -0.3538095943525299 1103 90-91 -0.09694027219078194 1103 92-93 -0.1304106838186847 1103 94-95 -0.22407512994298884 1103 96-97 -0.3374663039489114 1103 98-99 -0.44582072976628595 1103 100-101 -1.2096284643029627 1104 1 0.012827586558671555 1104 2 0.0957108208871702 1104 3 0.14286983414445587 1104 4 0.11400283279471779 1104 5 0.05342949650984252 1104 6 0.10782685290269711 1104 7 0.14714770760785 1104 8 0.06692171901133293 1104 9 0.11438007883812418 1104 10-11 0.21182064244938204 1104 12-13 0.1393052822050933 1104 14-15 0.02721098898248897 1104 16-17 0.16100363990136657 1104 18-19 0.22842169889171515 1104 20-21 0.20185007281719436 1104 22-23 0.18566986606406743 1104 24-25 0.17914491398958177 1104 26-27 0.09501348492329242 1104 28-29 0.16872041652683833 1104 30-31 0.18134414813012967 1104 32-33 0.23142879406344008 1104 34-35 0.20393661740676094 1104 36-37 0.27614392925868714 1104 38-39 0.3331177722951324 1104 40-41 0.16757394356491773 1104 42-43 0.11022088880103098 1104 44-45 0.09104002036192327 1104 46-47 0.18467801613502388 1104 48-49 0.2030082365833792 1104 50-51 0.11220615397822087 1104 52-53 0.144478869000082 1104 54-55 0.08137409807198281 1104 56-57 0.14515299398598103 1104 58-59 0.0023078330310752904 1104 60-61 0.20336953076257913 1104 62-63 9.210285828515907E-4 1104 64-65 0.1557628190047211 1104 66-67 0.11542554132882543 1104 68-69 0.03017397426172863 1104 70-71 0.18696607897135564 1104 72-73 -0.03988260284920386 1104 74-75 -0.0933455224764792 1104 76-77 -0.2535048448534951 1104 78-79 0.07263575610235051 1104 80-81 0.07978331362873803 1104 82-83 0.23111531055437595 1104 84-85 0.03186244402439442 1104 86-87 0.06174961809660928 1104 88-89 0.38925690451430484 1104 90-91 0.3931622941646644 1104 92-93 0.043823686376345705 1104 94-95 0.0655454856618789 1104 96-97 -0.25198400433318113 1104 98-99 -0.1550935655748411 1104 100-101 -0.7877295525038299 1105 1 0.034082914900615435 1105 2 0.08797818323326823 1105 3 0.09643382707852766 1105 4 0.11329941476846273 1105 5 0.07726683530781031 1105 6 0.05621491940100043 1105 7 0.13866154951539045 1105 8 0.05539320354581179 1105 9 0.16464123146148069 1105 10-11 0.24351190642410359 1105 12-13 0.19258026855094101 1105 14-15 0.1712777472224758 1105 16-17 0.23154535611237037 1105 18-19 0.2673041472229798 1105 20-21 0.3641952824064987 1105 22-23 0.35045967652316534 1105 24-25 0.30232814347201753 1105 26-27 0.380847995167251 1105 28-29 0.36092343943852256 1105 30-31 0.3168235513260669 1105 32-33 0.5419898369079448 1105 34-35 0.6075090682233082 1105 36-37 0.6947490880213323 1105 38-39 0.6384093807363556 1105 40-41 0.6506281667766274 1105 42-43 0.6873907573094797 1105 44-45 0.7314460780908192 1105 46-47 0.9172792093661428 1105 48-49 0.8291036067732378 1105 50-51 0.68628753120921 1105 52-53 0.8234972684909252 1105 54-55 0.5980383588307276 1105 56-57 0.6947772010905027 1105 58-59 0.690932750002446 1105 60-61 0.6745246790830848 1105 62-63 0.5914283080665115 1105 64-65 0.7436412100327203 1105 66-67 0.937278725386733 1105 68-69 0.781101985032258 1105 70-71 0.7077801588405528 1105 72-73 0.48458944918190383 1105 74-75 0.5711005159853109 1105 76-77 0.4528771607411102 1105 78-79 0.40907047836384436 1105 80-81 0.7272384335248034 1105 82-83 0.7292573047593613 1105 84-85 0.6716159302345766 1105 86-87 0.6916812952723816 1105 88-89 0.5058985447779953 1105 90-91 0.5013532448753004 1105 92-93 0.3192609539395974 1105 94-95 0.4466802408123769 1105 96-97 0.2850959973137499 1105 98-99 0.26245249764051337 1105 100-101 -0.4305350693250922 1106 1 -0.013252974793054761 1106 2 0.08975367095803932 1106 3 0.0784951312782809 1106 4 0.020625415308991535 1106 5 0.08133174273118016 1106 6 0.09215928365242831 1106 7 0.13032226701572824 1106 8 0.0769731216301821 1106 9 0.09087774371581503 1106 10-11 0.07769498495262894 1106 12-13 0.15742817651057806 1106 14-15 0.0548367688897855 1106 16-17 -0.004281022277410784 1106 18-19 0.01995039815572852 1106 20-21 0.0834452172150435 1106 22-23 0.1584934972369041 1106 24-25 0.08994908016766345 1106 26-27 0.1023066025065944 1106 28-29 0.050304521897054144 1106 30-31 0.11947628578140268 1106 32-33 -0.04513564466579112 1106 34-35 0.02577725996647473 1106 36-37 -0.05121058494314212 1106 38-39 0.1484799775101706 1106 40-41 0.08730647528494728 1106 42-43 0.05549429607786038 1106 44-45 0.10375820674290281 1106 46-47 0.20397202861550312 1106 48-49 0.042747313220232286 1106 50-51 0.13645987891410982 1106 52-53 0.15394127816728798 1106 54-55 -0.0834665498845979 1106 56-57 0.047165678564653035 1106 58-59 0.028897170900386016 1106 60-61 0.055000583890716825 1106 62-63 0.05705423873698834 1106 64-65 0.17979846181710712 1106 66-67 0.06293153061459122 1106 68-69 -0.3623294466102749 1106 70-71 -0.4206246998590295 1106 72-73 -0.28860244361245435 1106 74-75 -0.3866922269342332 1106 76-77 -0.36319622798478335 1106 78-79 -0.19366533067765346 1106 80-81 -0.01774894854277065 1106 82-83 -0.23101223878639487 1106 84-85 -0.31596088050878635 1106 86-87 -0.4997358032728876 1106 88-89 -0.6251296469081815 1106 90-91 -0.27818181293254796 1106 92-93 -0.30581617148022744 1106 94-95 -0.4333280428970703 1106 96-97 -0.7077547909448505 1106 98-99 -0.7343292527395953 1106 100-101 -1.0594647846153293 1107 1 0.13287135851664544 1107 2 0.039518739236299893 1107 3 0.05543433171797574 1107 4 0.0641272905456276 1107 5 0.06837168645322578 1107 6 0.05063436615310479 1107 7 0.04425601900438636 1107 8 0.08993781212693364 1107 9 0.10825374708211655 1107 10-11 0.11574956770841993 1107 12-13 0.19991932862834005 1107 14-15 0.20229761089953513 1107 16-17 0.19594086411842682 1107 18-19 0.2120299621313464 1107 20-21 0.12855817861524343 1107 22-23 0.12392013963880544 1107 24-25 0.09741781888623535 1107 26-27 0.02266222318837663 1107 28-29 0.04377722472638368 1107 30-31 0.04301237082117382 1107 32-33 0.0875367100410287 1107 34-35 -0.001047452097921564 1107 36-37 0.12955360700434682 1107 38-39 -0.06506644647172521 1107 40-41 0.12037510204079638 1107 42-43 0.011185139927384569 1107 44-45 0.06737078419808284 1107 46-47 0.00746910280146551 1107 48-49 -0.10271594877857382 1107 50-51 -0.18090092421454784 1107 52-53 -0.0749036288957754 1107 54-55 -0.31074138362041737 1107 56-57 -0.17511578442114484 1107 58-59 -0.1831695470207393 1107 60-61 -0.1537327404522344 1107 62-63 -0.2152596406092755 1107 64-65 -0.295374869551317 1107 66-67 -0.3782305738953795 1107 68-69 -0.6551553051804078 1107 70-71 -0.6079034448755074 1107 72-73 -0.5191770728401934 1107 74-75 -0.6393924703697458 1107 76-77 -0.8161153455739125 1107 78-79 -0.5321945501222771 1107 80-81 -0.5783615181197739 1107 82-83 -0.8218413051627742 1107 84-85 -0.6549342898247694 1107 86-87 -0.5275543235575562 1107 88-89 -0.6352493904875907 1107 90-91 -0.4922361472942143 1107 92-93 -0.2861967427959904 1107 94-95 -0.23306769248524972 1107 96-97 -0.16159495951919567 1107 98-99 -0.3995220061189819 1107 100-101 -0.9223329211635125 1108 1 0.024594136181065096 1108 2 -0.056414730390415 1108 3 0.08577227795544218 1108 4 0.04334188327246835 1108 5 0.07014136664388104 1108 6 0.05607276541269357 1108 7 0.03137082684005321 1108 8 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64-65 -0.420616026294347 2316 66-67 -0.4633128310651742 2316 68-69 -0.22671440607590654 2316 70-71 -0.1972569711342338 2316 72-73 0.035964822253038875 2316 74-75 -0.18933657562385164 2316 76-77 -0.20126572183070124 2316 78-79 -0.11081881434340346 2316 80-81 -0.25185644881197433 2316 82-83 -0.39825810629719527 2316 84-85 -0.4094160704981711 2316 86-87 -0.4440101414165625 2316 88-89 -0.5357041359768075 2316 90-91 -0.974519789102473 2316 92-93 -0.9060816147944912 2316 94-95 -0.7594866012646904 2316 96-97 -0.7423025886883288 2316 98-99 -0.5786376045468451 2316 100-101 0.49583801390584625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 13.0 5 38.0 6 89.0 7 190.0 8 442.0 9 879.0 10 1494.0 11 2498.0 12 3796.0 13 5411.0 14 6880.0 15 9425.0 16 12770.0 17 19099.0 18 18551.0 19 11855.0 20 10481.0 21 10884.0 22 11515.0 23 12525.0 24 14350.0 25 16717.0 26 19462.0 27 23058.0 28 27591.0 29 33455.0 30 40818.0 31 50014.0 32 62635.0 33 80768.0 34 112327.0 35 165488.0 36 249039.0 37 338373.0 38 179919.0 39 5854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22245036939869 19.862935590551487 15.333895576171127 21.58071846387869 2 15.140385138498965 21.61874676014556 40.308793414049546 22.932074687305928 3 19.381001750162955 26.33802780757446 30.60257443325002 23.67839600901257 4 12.697534887779142 17.60594644809304 37.576601707050436 32.11991695707738 5 13.019659821698943 38.59147809216841 33.81432875348365 14.57453333264901 6 29.940810104753158 37.214315409132666 17.644696390353058 15.200178095761116 7 27.133492267975097 34.553208033299285 20.786136245823474 17.52716345290214 8 25.52479226438239 34.986322040248616 19.94005306945735 19.54883262591165 9 26.507398416128186 17.74696031082073 20.964488629073237 34.78115264397785 10-11 24.581962543433296 27.289197747884153 26.895411082996727 21.233428625685825 12-13 26.420211046043146 24.886733405529693 27.61440214741402 21.078653401013145 14-15 23.099866362394646 25.903208649811095 27.251646090666988 23.74527889712727 16-17 20.92476800763727 29.365831938983955 27.968039459231964 21.741360594146812 18-19 20.803682784247506 27.822233998326823 30.899582475787703 20.474500741637968 20-21 22.084580089201854 26.542555648509797 30.522219627487313 20.850644634801043 22-23 21.54631516277542 26.524207165916476 30.429611114817874 21.49986655649023 24-25 21.537981852318406 26.76685197998475 30.710173114139465 20.98499305355738 26-27 21.551007890797877 26.618819735784342 30.998023290653265 20.83214908276452 28-29 20.868679033932835 27.003938830245367 31.262199579588657 20.865182556233144 30-31 21.103706136861717 27.046753986624854 30.759627179363473 21.089912697149956 32-33 20.938922648956318 27.100901256935213 30.59686459076468 21.363311503343787 34-35 21.568155656763594 26.734609940545624 30.49481362279003 21.202420779900752 36-37 21.77595597731484 26.461772939845822 30.723053351836548 21.039217731002783 38-39 21.58343921251556 26.67625996226851 30.768907455177814 20.971393370038115 40-41 21.33618338908971 26.926514501150418 30.835652773126064 20.901649336633803 42-43 21.108242734589766 27.311827887998362 30.052359010667818 21.527570366744055 44-45 21.29788332241039 27.397249727407548 29.673103986762868 21.6317629634192 46-47 21.565979938041405 27.482393897427954 29.17029567473566 21.78133048979499 48-49 21.77240463180951 27.391410487172767 29.058134361227122 21.7780505197906 50-51 21.746472132323387 27.890644522522507 28.917688594283746 21.445194750870357 52-53 21.695573011563393 28.05361093004994 28.717299924553092 21.533516133833576 54-55 21.19047649597873 27.69254358726949 28.984539799526786 22.13244011722499 56-57 21.687150472409574 28.154417335862775 28.315736528452035 21.842695663275617 58-59 21.800458785491962 27.536368101095764 28.589117526009982 22.074055587402295 60-61 21.68084759659863 27.502107571474582 29.10232211391023 21.71472271801656 62-63 21.876239027588692 27.479306234369805 28.92928127738692 21.71517346065458 64-65 21.561444305186605 27.606382279131523 28.989590093282082 21.842583322399793 66-67 21.32703823891333 27.74184980310187 28.709210521250757 22.221901436734047 68-69 21.28654445483461 27.90455736037841 28.77929851969595 22.02959966509103 70-71 21.40880690366194 27.754186317009783 28.5593718904555 22.27763488887278 72-73 21.529378101920045 27.503477229918033 28.385198805167345 22.581945862994576 74-75 21.28568330775666 28.15677302798721 28.1775273430884 22.380016321167734 76-77 21.49410698328982 27.47760518794817 28.570735947193228 22.45755188156878 78-79 21.351847336473185 27.655441910604058 28.598135338731822 22.394575414190932 80-81 21.21928139064159 28.160395969731283 28.397868743604466 22.22245389602266 82-83 21.299701291835824 27.870190259650272 28.241650798864704 22.588457649649197 84-85 21.216459487063677 27.613728514311813 28.18265978577184 22.987152212852664 86-87 21.347610328707844 27.532902574470313 28.395148150693245 22.724338946128597 88-89 21.450289135376682 27.216216059526392 28.33143410760332 23.0020606974936 90-91 21.10459385810641 27.331909630288152 28.579904528148738 22.9835919834567 92-93 21.09751512787481 27.610713204235292 28.419201237945174 22.872570429944723 94-95 20.92998637977149 27.424070273450308 28.30579241437316 23.340150932405045 96-97 20.800475007570356 27.143596764508132 28.303400499899915 23.752527728021597 98-99 20.88134588476218 27.174510668137113 28.024734603745284 23.919408843355423 100-101 21.3635862054807 26.153617167060368 28.951162053652197 23.531634573806734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1615.0 1 1063.5 2 662.0 3 859.5 4 748.0 5 711.0 6 1257.5 7 2611.0 8 4108.0 9 4517.5 10 3747.0 11 3029.5 12 2938.5 13 2973.5 14 3103.5 15 3237.0 16 3223.0 17 3305.5 18 3463.0 19 3646.0 20 4016.5 21 4581.0 22 5277.5 23 6217.0 24 7306.0 25 8621.5 26 10776.5 27 13405.0 28 15420.5 29 17602.0 30 20149.0 31 22797.0 32 26292.0 33 29564.0 34 32982.0 35 36049.0 36 39235.5 37 42748.0 38 44106.5 39 44149.5 40 44498.0 41 45875.0 42 47540.0 43 49587.0 44 51851.5 45 53338.5 46 54017.5 47 58264.0 48 76217.0 49 76671.0 50 59402.0 51 52192.0 52 46913.0 53 44753.0 54 43425.0 55 40231.0 56 36476.5 57 32765.0 58 29610.5 59 26458.0 60 22670.0 61 18970.5 62 15654.0 63 12061.0 64 8781.5 65 6710.5 66 5109.5 67 4135.5 68 3193.5 69 2277.5 70 1789.0 71 1351.0 72 1067.0 73 949.0 74 800.0 75 660.5 76 471.0 77 271.0 78 169.0 79 104.0 80 55.0 81 27.5 82 18.0 83 11.0 84 3.5 85 1.5 86 1.5 87 1.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42310574371660703 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012189551373185039 16-17 0.0025662213417231663 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.207776677153958E-5 26-27 0.003913487546127829 28-29 3.207776677153958E-5 30-31 0.0 32-33 0.0 34-35 0.013216039909874307 36-37 0.027234023989037102 38-39 0.02129963713630228 40-41 0.009366707897289557 42-43 0.009334630130518017 44-45 0.004266342980614764 46-47 0.005806075785648663 48-49 0.0035606321116408934 50-51 9.944107699177268E-4 52-53 0.0 54-55 0.0 56-57 6.415553354307915E-5 58-59 0.0020208993066069937 60-61 0.0029511545429816415 62-63 0.0019246660062923746 64-65 0.0033681655110116555 66-67 0.0024379102746370077 68-69 0.005100364916674793 70-71 1.9246660062923748E-4 72-73 0.0 74-75 0.0 76-77 0.09543135614533026 78-79 0.01616719445285595 80-81 4.490887348015541E-4 82-83 0.0 84-85 0.0 86-87 0.10361118667207284 88-89 0.4057837496599757 90-91 0.4120068364136543 92-93 0.0 94-95 6.415553354307915E-5 96-97 0.0 98-99 3.207776677153958E-4 100-101 3.8493320125847496E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558712.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.84130808438402 #Duplication Level Percentage of deduplicated Percentage of total 1 70.22454428004505 32.89409513707058 2 14.974691056771466 14.028682345174046 3 5.963469583494814 8.380101480370012 4 2.964692769599288 5.554803495853839 5 1.6475056390159775 3.8585659603953673 6 1.0060336583534322 2.8274359520515824 7 0.661313708539141 2.1683759413475903 8 0.4613764444660641 1.7289180942490077 9 0.3262607486928242 1.3754232220826128 >10 1.5906252400931427 13.383167881131882 >50 0.11882174728544065 3.788197282833994 >100 0.054228992270738796 4.541840228541129 >500 0.003149596203706644 1.0554895637790944 >1k 0.002738779307570995 2.433880036951319 >5k 4.108168961356493E-4 1.2851892373064682 >10k+ 1.3693896537854975E-4 0.6958341408614778 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10848 0.6959592278753227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8605 0.5520583661381961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5982 0.3837784016546995 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5449 0.3495835022762383 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4475 0.2870960126052792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4302 0.2759971053023265 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2945 0.1889380462843681 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2124 0.13626635324550013 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2086 0.13382844297086313 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2069 0.13273779890063078 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1992 0.12779782281781368 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1991 0.1277336672842706 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1889 0.12118980286287652 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1661 0.10656234121505448 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.10341872007144359 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1574 0.1009808097968066 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.415553354307915E-5 0.0 10-11 0.0 0.0 0.0 9.623330031461873E-5 0.0 12-13 0.0 0.0 0.0 2.566221341723166E-4 0.0 14-15 0.0 0.0 0.0 2.886999009438562E-4 0.0 16-17 0.0 0.0 0.0 3.207776677153958E-4 0.0 18-19 0.0 0.0 0.0 3.8493320125847496E-4 0.0 20-21 0.0 0.0 0.0 6.736331022023311E-4 0.0 22-23 0.0 0.0 0.0 0.0010906440702323458 0.0 24-25 0.0 0.0 0.0 0.0017001216388915978 0.0 26-27 0.0 0.0 0.0 0.002822843475895483 0.0 28-29 0.0 0.0 0.0 0.006640097721708692 0.0 30-31 0.0 0.0 0.0 0.01757861619080369 0.0 32-33 0.0 0.0 0.0 0.03672904295341282 0.0 34-35 0.0 0.0 0.0 0.06226294530355832 0.0 36-37 0.0 0.0 0.0 0.10078834319617735 0.0 38-39 0.0 0.0 0.0 0.15657157961188467 0.0 40-41 0.0 0.0 0.0 0.22114412412299383 0.0 42-43 0.0 0.0 0.0 0.2854279687331592 0.0 44-45 0.0 0.0 0.0 0.36279954218611266 0.0 46-47 0.0 0.0 0.0 0.46063673083930834 0.0 48-49 0.0 0.0 0.0 0.5714012595014346 0.0 50-51 0.0 0.0 0.0 0.6920778180959664 0.0 52-53 0.0 0.0 0.0 0.8115354215531798 0.0 54-55 0.0 0.0 0.0 0.9470639861629345 0.0 56-57 0.0 0.0 0.0 1.1018071330688413 0.0 58-59 0.0 0.0 0.0 1.2774329061430207 6.415553354307915E-5 60-61 0.0 0.0 0.0 1.4858100790909416 6.415553354307915E-5 62-63 0.0 0.0 0.0 1.6950854295084659 6.415553354307915E-5 64-65 0.0 0.0 0.0 1.9100706224113242 6.415553354307915E-5 66-67 0.0 0.0 0.0 2.1614320028331084 6.415553354307915E-5 68-69 0.0 0.0 0.0 2.440123640544244 6.415553354307915E-5 70-71 0.0 0.0 0.0 2.7595219642884636 6.415553354307915E-5 72-73 0.0 0.0 0.0 3.090532439603981 6.415553354307915E-5 74-75 0.0 0.0 0.0 3.42388459189382 6.415553354307915E-5 76-77 0.0 0.0 0.0 3.7811667581952277 6.415553354307915E-5 78-79 0.0 0.0 0.0 4.188939329395039 6.415553354307915E-5 80-81 0.0 0.0 0.0 4.648613727231201 6.415553354307915E-5 82-83 0.0 0.0 0.0 5.116147177926391 6.415553354307915E-5 84-85 0.0 0.0 0.0 5.610433486109043 6.415553354307915E-5 86-87 0.0 0.0 0.0 6.134487961855686 6.415553354307915E-5 88-89 0.0 0.0 0.0 6.681638429677836 6.415553354307915E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8105 0.0 23.187943 12-13 GTACATG 9055 0.0 22.836235 1 AGTACTT 8485 0.0 22.233372 12-13 TACATGG 9205 0.0 21.9609 2 ACATGGG 9055 0.0 21.224178 3 GTACTTT 8940 0.0 21.128351 14-15 GACCGTC 245 1.7753882E-7 19.368599 7 CTACCGT 150 7.7742507E-4 18.981228 6 TACTTTT 10050 0.0 18.841995 14-15 ATGGGAT 2125 0.0 18.53461 5 CATGGGA 5600 0.0 18.472801 4 ACTTTTT 10270 0.0 18.390682 16-17 GTAAGCG 260 0.0 18.251766 94-95 TACCGTG 185 1.5333357E-4 17.955217 7 CATGGGG 3305 0.0 17.660295 4 TATACAG 1030 0.0 17.04625 5 GTATCAA 20980 0.0 16.987103 1 ATGGGGA 1820 0.0 16.426062 5 ACCGTCC 175 0.00218458 16.269623 8 AGTACAT 7215 0.0 16.179426 2 >>END_MODULE